psiCHECK-2 Vector from Promega

Product Specs
ItempsiCHECK-2 Vector
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberC8021
Hostmammalian cell line of choice
PromoterSV40 early enhancer/promoter
ResistanceAmp
Quantity20microg
Molecular Weight~78kDa
Nucleic Acid TypeExpression Vectors
Expression System (standardized)Mammalian
Selectable Marker (standardized)Ampicillin
Vector NamepsiCHECK-2

Specifications/Features

Vector Size:
~6.3 kb

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(41) E2F1-driven EXOSC10 transcription promotes hepatocellular carcinoma growth and stemness: a potential therapeutic targetHereditasApril 12, 2025Haoyue Deng, Dingyong Wu, Yongpeng He, Xiaolei Yu, Jifei Liu, Yanrui Zhang, Bing Leng, Xiaofeng Yuan, Liguo Xiaomutated (mut) sequences were amplified and inserted into the NotI and XhoI of psiCHECK-2 vectors (#C8021, Promega, Beijing, China) to establish the EXOSC10-wt/mut vectors. Two hours before transfection, Huh-7

(42) Protocol for extracellular vesicle secretion-related gene screening via ExoScreen techniqueSTAR protocolsMarch 21, 2025Yamamoto T, Urabe F, Yoshioka Y, Yamamoto Y, Ochiya T.this study,N/A ,, Recombinant DNA,Recombinant DNA,Recombinant DNA ,, psiCHECK-2 plasmid,Promega,C8021 ,, Software and algorithms,Software and algorithms,Software and algorithms ,, GraphPad Prism,GraphPad

(43) A double-agent microRNA regulates viral cross-kingdom infection in animals and plantsThe EMBO JournalMarch 5, 2025Wan Zhao, Hong, Lu, Jiaming Zhu, Lan Luo, Feng Cuiwere PCR amplified and cloned into the XhoI and NotI restriction sites of psiCHECK2 vector (Promega; C8021). For transfection, Drosophila S2 cells seeded in 24-well plates for 16 h were cotransfected with 1

(44) PIWI-interacting RNA MIABEPIR regulates cerebral endothelial cell function via DAPK2 pathway in offspring following maternal immune activationClin Transl MedFebruary 25, 2025Shan-Shan Li, Miao Guo, Yao Long, Yuang Cai, Ying Zhao, Shaoyuan Huang, Houzhi Yang, Yonggang Fan, Xu Chen, Xin Jininteraction between piRNA MIABEPIR and the DAPK2 3′‐UTR. We utilized the pSI‐Check2 vector (Promega, C8021) for the assay. The 3′‐UTR of DAPK2 was cloned downstream of the Renilla luciferase gene using the XhoI

(45) A general framework to over-express tRNA-derived fragments from their parental tRNAs in mammalian cellsMethods in EnzymologyFebruary 14, 2025Fatemeh Esmaeili, Kumarjeet Banerjee, Zhangli Su, Anindya Duttapipet tips (10 μL, 20 μL, 200 μL and 1000 μL)Vectors; pcDNA3.1 Vector (Addgene) and psiCHECK-2 (Promega#C8021)Restriction enzymes; BamHI-HF (NEB) and XhoI (NEB)10X r CutSmart buffer (NEB)Forward and reverse primers

(46) NE-MTOC Formation in Skeletal Muscle Is Mbnl2-Dependent and Occurs in a Sequential and Gradual MannerCellsFebruary 7, 2025Payel Das, Robert Becker, Silvia Vergarajauregui, Felix B. Engelamplification, the 3′ UTR fragment was cloned into the psiCHECK-2 vector (Promega GmbH (Walldorf, Germany) C8021) between the Renilla luciferase ORF (hRluc) and the synthetic poly(A) signal using NEBuilder HiFi DNA Assembly Master Mix

(47) Characterization and molecular targeting of CFIm25 (NUDT21/CPSF5) mRNA using miRNAsFASEB JJanuary 31, 2025Naazneen Khan, Mahesh Gupta, Chioniso Patience Masamhaassays The full‐length 3′UTR of NUDT21 was cloned into the psiCHECK2 dual luciferase vector (Cat# C8021, Promega, Madison, WI, USA) downstream of the synthetic Renilla luciferase gene (Rluc) between Xho1

(48) Characterization and molecular targeting of CFIm25 (NUDT21/CPSF5) mRNA using miRNAsFASEB JJanuary 15, 2025Naazneen Khan, Mahesh Gupta, Chioniso Patience Masamhaassays The full‐length 3′UTR of NUDT21 was cloned into the psiCHECK2 dual luciferase vector (Cat# C8021, Promega, Madison, WI, USA) downstream of the synthetic Renilla luciferase gene (Rluc) between Xho1

(49) Small interfering RNAs generated from the terminal panhandle structure of negative-strand RNA virus promote viral infectionPLoS PathogJanuary 3, 2025Wan Zhao, Qiong Li, Mengqi Sun, Lan Luo, Xiaoming Zhang, Feng CuiRdRP coding region) were cloned and inserted into the luciferase reporter vector psiCHECK2 (Promega; C8021). For cellular target validation, the putative target of vsiR-8401 (131 bp sequence from the open reading

(50) miRNA‑22‑3p inhibits cell viability and metastasis of nasopharyngeal carcinoma by targeting FOXP1Oncology LettersDecember 10, 2024Ying Jin, Zhijun Wang, Yuanshan Liang, Yiting Jiang, Fayang Yuan, Tian Zhangregion (3′UTR) sequences were cloned into the luciferase reporter plasmid psiCHECK-2 vector (cat. no. C8021; Promega Corporation). Subsequently, the luciferase reporter gene plasmid and either miRNA-22-3p mimic

(51) miR-10a regulates cell death and inflammation in adipose tissue of male mice with diet-induced obesityMolecular metabolismDecember 1, 2024Lee S, Cho YK, Kim H, Choi C, Kim S, Lee YH.downstream of the stop codon of the hRluc (Renilla luciferase) gene in the psiCHECK2 vector (Promega, cat#C8021). Putative miRNA target sequences used for plasmids construction for the dual luciferase assay are indicated

(52) SCAR-6 elncRNA locus epigenetically regulates PROZ and modulates coagulation and vascular functionEMBO reportsNovember 1, 2024Ranjan G, Sehgal P, Scaria V, Sivasubbu S.DNA Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP,Addgene,118156 E1b-GFP-Tol2,Addgene,37845 TOPO pCR2.1-luciferase -PAR2-GFP,This study, psi-CHEK-2,Promega,C8021 Antibodies,Antibodies,Antibodies CTCF,Abcam,ab70303 IgG,Abcam,ab231712 Suz12,Cell Signaling Technology,ab3737 PRDM14,Abcam,ab187881 NF-kβ

(53) Stage-specific expression patterns and co-targeting relationships among miRNAs in the developing mouse cerebral cortexCommunications BiologyOctober 22, 2024Hristo Todorov, Stephan Weißbach, Laura Schlichtholz, Hanna Mueller, Dewi Hartwich, Susanne Gerber, Jennifer Wintersites downstream of the synthetic Renilla luciferase gene of the psiCHECK-2 vector (Promega; CatNo. C8021). Mutations in the miRNA binding sites were introduced using the QuikChange II XL system (Agilent Technologies;

(54) Possible involvement of a MEG3-miR-21-SPRY1-NF-κB feedback loop in spermatogenic cells proliferation, autophagy, and apoptosisiScienceOctober 18, 2024Xingyu Fang, Xiaotong Lu, Yujie Ma, Ning Sun, Yunyun Jiao, Hui Meng, Mengjiao Song, Haixia Jin, Guidong Yao, Ning Song, et al.DNA,Recombinant DNA,Recombinant DNA ,, pcDNA3.1-MEG3,Synbio technologies,N/A psiCHECK™ -2,Promega,C8021 pRL-TK,Promega,E2241 pGL3-basic,Promega,N/A pcDNA3.1-SPRY1,pcDNA3.1-MEG3,N/A ,, Software and algorithms,Software

(55) MiR-34b promotes oxidative stress and induces cellular senescence through TWIST1 in human cervical cancerTranslational OncologyOctober 1, 2024K.J. Sindhu, Venkatesan Nalini, G.K. Suraishkumar, Devarajan Karunagaranbinding sites for miR-34b was amplified from genomic DNA and cloned into psiCheck2 vector (Cat No- C8021, Promega). The mutant (MUT) miR-34b binding site in TWIST1 3′ UTR was generated via site-directed mutagenesis

(56) Physical exercise‐induced circAnks1b upregulation promotes protective endoplasmic reticulum stress and suppresses apoptosis via miR‐130b‐5p/Pak2 signaling in an ischemic stroke modelCNS Neuroscience & TherapeuticsSeptember 27, 2024Xiaofeng Yang, Yating Mu, Yifeng Feng, Mingyue Li, Haojie Hu, Xiaoya Zhang, Zejie Zuo, Rui Wu, Jinghui Xu, Fang Zheng, et al.amplified separately by PCR and inserted into the XhoI/NotI‐digested dual reporter vector pSI‐Check2 (C8021‐1, Promega). Human embryonic kidney 293 T (HEK293T) cells were seeded in 96‐well culture plates for

(57) HAND2-AS1 plays a tumor-suppressive role in hepatoblastoma through the negative regulation of CDK1HeliyonSeptember 15, 2024Keke Chen, Yalan You, Wenfang Tang, Xin Tian, Chengguang Zhu, Zexi Yin, Minhui Zeng, Xiangling Hewas synthesized and cloned into the downstream of Renilla of psiCHECK-2 luciferase reporter plasmid (C8021, Promega, Madison, USA) according to manufacturer's guidance and previous research [22]. Then, HuH-6

(58) Deletion of miR-146a enhances therapeutic protein restoration in model of dystrophin exon skippingMolecular Therapy - Nucleic AcidsSeptember 10, 2024Nikki M. McCormack, Kelsey A. Calabrese, Christina M. Sun, Christopher B. Tully, Christopher R. Heier, Alyson A. Fiorillowas gel purified, digested with XhoI and NotI, and ligated into the pSiCheck2 plasmid (Promega Cat #C8021) where Renilla luciferase is upstream of the 3′UTR and firefly luciferase is driven by a separate promoter

(59) miR-497 target gene regulatory network in angiosarcomaMol Cancer ResSeptember 4, 2024Annaleigh Benton, Noah M. Moriarty, Emma Terwilliger, Bozhi Liu, Ant Murphy, Hannah Maluvac, Mae Shu, Lauren E. Gartenhaus, Nimod D. Janson, Claire M. Pfeffer, et al.-293T cells with 100 ng pCMV6-empty or pCMV6-miR-497/195 with 10 ng of psiCHECK2 reporter plasmids (Cat# C8021, Promega) using FuGENE6 (Cat# E2691; Promega) and performing the Dual-Luciferase Reporter Assay System

(60) miR-497 Target Gene Regulatory Network in AngiosarcomaMolecular Cancer ResearchSeptember 4, 2024Annaleigh Benton, Noah M. Moriarty, Emma Terwilliger, Bozhi Liu, Ant Murphy, Hannah Maluvac, Mae Shu, Lauren E. Gartenhaus, Nimod D. Janson, Claire M. Pfeffer, et al.-293T cells with 100 ng pCMV6-empty or pCMV6-miR-497/195 with 10 ng of psiCHECK2 reporter plasmids (Cat# C8021, Promega) using FuGENE6 (Cat# E2691; Promega) and performing the Dual-Luciferase Reporter Assay System

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