psiCHECK-2 Vector from Promega

Product Specs
ItempsiCHECK-2 Vector
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberC8021
Hostmammalian cell line of choice
PromoterSV40 early enhancer/promoter
ResistanceAmp
Quantity20microg
Molecular Weight~78kDa
Nucleic Acid TypeExpression Vectors
Expression System (standardized)Mammalian
Selectable Marker (standardized)Ampicillin
Vector NamepsiCHECK-2

Specifications/Features

Vector Size:
~6.3 kb

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(21) A portable poison exon for small-molecule control of mammalian gene expressionNature MethodsNovember 3, 2025Qian Hou, Maxim Oleynikov, Xiaohan Mei, Linghao Dong, Timo Hagen, Samie R. JaffreyExtraction Kit (catalog number 28706). The psiCHECK-2 vector was purchased from Promega (catalog number C8021). pINDUCER20 (Plasmid #44012), pcDNA3-EGFP (Plasmid #13031), pcDNA3-mCherry (Plasmid #30125), pCMV-Cre

(22) An interplay of non-coding RNAs regulates CDH13 expression and affects endothelial function and coronary artery disease riskResearch SquareOctober 31, 2025Shuangyue Li, Xiaoning Song, Anastasiia Diagel, Ling Li, Aldo Moggio, Yifan Chen, Zhaolong Li, Tan Dang, Miaomiao Li, Rui Shen, et al.via GenScript Biotech (Netherlands) B.V. and inserted into the psiCHECK™−2 Vector backbone (Promega, C8021) via restriction enzyme cloning. For the assay, HEK.293T cells were seeded in 12-well plates and incubated

(23) A microRNA CRISPR screen reveals microRNA-483-3p as an apoptotic regulator in prostate cancer cellsCell Death DisOctober 24, 2025Jonathan Tak-Sum Chow, Ayeisha Desjardins, Daniel K. C. Lee, Iulia A. Grigore, Linda Lee, Norman J. Fu, Stephanie Chau, Byeong Yeop Lee, Martino Marco Gabra, Leonardo SalmenaLSB-hsa-miR-483-3p (Addgene #103550) and all 3’UTR sequences were cloned into the psiCHECK-2 vector (Promega, C8021). Lentivirus was made using the pMDG.2 (Addgene #12259) and psPAX2 (Addgene #12260) vectors. Plasmid cloning

(24) Promoting ethical and reproducible cell culture: implementing animal-free alternatives to teaching in molecular and cell biologyFront ToxicolOctober 1, 2025Alexandra Nessar, Viola Röhrs, Mathias Ziersch, Ahmed S. M. Ali, Julia Moradi, Anke Kurreck, Johanna Berg, Jens Kurreckdual-luciferase reporter assay. For this purpose, a reporter construct based on the psiCHECK™-2 vector (C8021, Promega, Madison, WI, United States) was used, in which the Renilla luciferase (RLuc) gene is fused

(25) miR-199a-5p/GPR89A axis modulates sorafenib resistance in hepatocellular carcinoma through glutamate metabolic reprogrammingScience ProgressSeptember 23, 2025Yao Li, Yi Cai, Guangliang Duan, Yulin Du, Junjie Hu, Xue Han, Xiaolong Tang, Liangxian Jiang, Junqi Ren, Qi Huomutant GPR89A 3′-untranslated region (3′-UTR) sequences were generated using the psiCHECK2 vector (C8021, Promega). These plasmids were co-transfected with miR-199a-5p mimics into Huh7/SR and Hep3B/SR cells

(26) Half of annotated human microRNAs are expressed at levels of questionable biological significanceiScienceSeptember 19, 2025Saba Ataei Kachooei, Julie M. Bracken, Katherine A. Pillman, Philip A. Gregory, Cameron P. Brackendetails Reporters construct design and cloning The PsiCHECK2 dual luciferase reporters (Promega, #C8021) were designed to incorporate a single copy of a target site that perfectly complements small RNA sequences

(27) Cancer Cell-Secreted miR-33a Reduces Stress Granule Formation by Targeting Polyamine Metabolism in Stroma to Promote TumourigenesisJournal of extracellular vesiclesSeptember 1, 2025Hu S, Li X, Hu Q, Wang C, Hua A, Deng G, Huang W, Fu X, Zhou H, Zhang X, et al.were cloned by PCR and inserted into the XhoI/NotI sites of psiCHECK‐2 reporter vector (Promega, Cat# C8021) downstream of the Renilla luciferase (R‐Luc) gene. Site‐directed mutagenesis was used to mutate the

(28) CircYAP1 inhibits osteosarcoma progression through the miR-135b-5p/FRK axisiScienceJuly 18, 2025Mingrui Du, Xuesan Zhang, Menglin Cong, Chengkui Cai, Qingxiang Zhang, Haixia Xin, Lanbo Yang, Qingqing Geng, Mingfu Shang, Zheng Zhang, et al.paper,N/A ,, Recombinant DNA,Recombinant DNA,Recombinant DNA ,, psiCHECK™-2 Vector,Promega,Cat# C8021 pcDNA3.1-circYAP1,Hanbio,N/A pcDNA3.1,Hanbio,N/A ,, Software and algorithms,Software and algorithms,Software

(29) MicroRNA Signatures in Lung Adenocarcinoma Metastases: Exploring the Oncogenic Targets of Tumor-Suppressive miR-195-5p and miR-195-3pCancers (Basel)July 15, 2025Yuya Tomioka, Naohiko Seki, Keiko Mizuno, Takayuki Suetsugu, Kentaro Tsuruzono, Yoko Hagihara, Mayuko Kato, Chikashi Minemura, Hajime Yonezawa, Kentaro Tanaka, et al.-Luciferase Reporter Assay The binding sequences of miR-195-5p and miR-195-3p, which were cloned into the psiCHEK2 vector (C8021; Promega, Madison, WI, USA), are shown in Figures S1 and S2. The procedures for the transfection and

(30) Hypoxia-inducible factor 1α in Schwann cells promotes peripheral nerve myelinationJ Biol ChemJuly 1, 2025Yuka Kobayashi-Ujiie, Shuji Wakatsuki, Yurika Numata-Uematsu, Megumi Shibata, Akihito Harada, Yasuyuki Ohkawa, Nobuhito Goda, Toshiyuki Arakidescribed. T7 and SV40-renilla luciferase expressing psiCHECK-2 vectors were purchased from Promega (C8021). The integrity of the plasmids was confirmed by sequence analysis. Gene transfection by electroporation

(31) Glomerular mesangial derived extracellular vesicles deteriorate diabetic kidney disease via miR-3147/PRKAR2B axisRen FailJune 26, 2025Jiayan Zhang, Fan Zhao, Yiying Tao, Miao Hu, Yujie Bai, Jianzhong Li, Lei Shen, Guoyuan Lu, Zhen Weng, Xiahong Shen, et al.(3’UTR) of target gene was synthesized by Genewiz. Co.Ltd (Suzhou, China), cloned into psiCHECK2 vector (C8021; Promega, Madison, WI, USA) and co-transfected into MCs with miRNA mimics in a 96-well plate. A Duo-Lite

(32) Glomerular mesangial derived extracellular vesicles deteriorate diabetic kidney disease via miR-3147/PRKAR2B axisRen FailJune 26, 2025Jiayan Zhang, Fan Zhao, Yiying Tao, Miao Hu, Yujie Bai, Jianzhong Li, Lei Shen, Guoyuan Lu, Zhen Weng, Xiahong Shen, et al.(3’UTR) of target gene was synthesized by Genewiz. Co.Ltd (Suzhou, China), cloned into psiCHECK2 vector (C8021; Promega, Madison, WI, USA) and co-transfected into MCs with miRNA mimics in a 96-well plate. A Duo-Lite

(33) 5′-tiRNA-Lys maintains intestinal epithelial homeostasis by EWSR1-dependent suppression of miR-125a and autophagy activationActa Biochimica et Biophysica SinicaJune 23, 2025Ningqin Xu, Ju Huang, Yiming Wang, Yaxin Liu, Yangwei Jiang, Wei Zhou, Jinghao Sheng, Lahong Zhanginserted into the XhoI and NotI restriction sites of the psiCHECK-2 dual-luciferase reporter vector (#C8021; Promega, Madison, USA). A mutant construct (mut), in which the miR-125a seed sequence was disrupted,

(34) Systematic analysis of siRNA and mRNA features impacting fully chemically modified siRNA efficacyNucleic Acids ResJune 23, 2025Sarah M Davis, Samuel Hildebrand, Hannah J MacMillan, Kathryn R Monopoli, Julianna Buchwald, Jacquelyn Sousa, David Cooper, Socheata Ly, Dimas Echeverria, Nicholas McHugh, et al.target mRNAs (see Supplementary Table S4) were cloned into psiCHECK-2 vectors (Promega, Madison, WI; C8021) according to the manufacturer’s protocol. HeLa cells were plated (36 or 66 cells/10-cm dish) and grown

(35) miR-1236-3p targets Toll-like receptor 4 to suppress the anti-Mycobacterium tuberculosis activity of macrophageiScienceJune 20, 2025Zhang Y, Han J, Yang Q, Cui X, Duan H, Wu T, Wu C, Xing L, Liu Q, Dong L.AGTGCAGGGTCCGAGGTATT,This paper,N/A ,, Recombinant DNA,Recombinant DNA,Recombinant DNA ,, psiCHECK™-2 Vectors,Promega,C8021 ,, Software and algorithms,Software and algorithms,Software and algorithms ,, EpiData 3.1,"EpiData

(36) Upstream open reading frames buffer translational variability during Drosophila evolution and developmentElifeJune 6, 2025Yuanqiang Sun, Yuange Duan, Peixiang Gao, Chenlu Liu, Kaichun Jin, Shengqian Dou, Wenxiong Tang, Hong Zhang, Jian Lureagent,pU6B,Tsinghua Fly Center,N/A,sgRNA-expression vector Recombinant DNA reagent,psiCHECK-2,Promega,C8021,Used for dual-luciferase reporter assay Commercial assay or kit,PrimeScript II 1st Strand cDNA Synthesis

(37) Upstream open reading frames buffer translational variability during Drosophila evolution and developmentElifeJune 6, 2025Yuanqiang Sun, Yuange Duan, Peixiang Gao, Chenlu Liu, Kaichun Jin, Shengqian Dou, Wenxiong Tang, Hong Zhang, Jian Lureagent,pU6B,Tsinghua Fly Center,N/A,sgRNA-expression vector Recombinant DNA reagent,psiCHECK-2,Promega,C8021,Used for dual-luciferase reporter assay Commercial assay or kit,PrimeScript II 1st Strand cDNA Synthesis

(38) miR-29a-3p/Vegfa axis modulates high phosphate-induced vascular smooth muscle cell calcificationRen FailApril 22, 2025Chen Fu, Qiaojing Liang, Lili Ma, Wenhu Liu, Weikang Guo, Gang Wangcontaining the predicted miR-29a-3p binding site, was cloned into the psiCHECK-2 vector (Promega, USA; Cat. #C8021). A negative control plasmid was constructed by mutagenizing the putative miR-29a-3p binding site using

(39) miR-29a-3p/Vegfa axis modulates high phosphate-induced vascular smooth muscle cell calcificationRen FailApril 22, 2025Chen Fu, Qiaojing Liang, Lili Ma, Wenhu Liu, Weikang Guo, Gang Wangcontaining the predicted miR-29a-3p binding site, was cloned into the psiCHECK-2 vector (Promega, USA; Cat. #C8021). A negative control plasmid was constructed by mutagenizing the putative miR-29a-3p binding site using

(40) "Chasing non-existent ""microRNAs"" in cancer"OncogenesisApril 18, 2025Orang A, Warnock NI, Migault M, Dredge BK, Bert AG, Bracken JM, Gregory PA, Pillman KA, Goodall GJ, Bracken CP.output kit. Reporter construct design and cloning PsiCHECK2 dual luciferase reporters (Promega, #C8021) were constructed to contain a single copy of a perfectly complementary target site of the small RNA

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