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Author Profile
Karl Wagner
PhD Candidate
University of Toronto
Articles by Karl Wagner
Suitable Agarose Beads for Extracellular Vesicle Chromatography
December 28, 2023
Extracellular vesicles (EVs) were isolated from conditioned cell culture media from various cell types via chromatography in ...
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Fast and Versatile Reagent From ThermoFisher for Embryoid Body Dissociation
December 28, 2023
Embryoid bodies (EBs) were formed from human embryonic stem cells for mesoderm differentiation. TryplE was used to dissociate EBs ...
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Robust GFP+ HUVECs for Visualizing Vascular Formation
December 27, 2023
GFP HUVECs were grown in 3D in vitro tissues. Cell culture supplements and co-cultures were used to analyze the effects on ...
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Cheap and Effective PEG From Sigma-Aldrich for Precipitation-Based Isolations
December 27, 2023
PEG buffer solution was prepared and used to isolate extracellular vesicles from conditioned cell culture media. Extracellular ...
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Efficient Labelled Primary Ki-67 Antibody for Assessing Cell Proliferation
December 13, 2023
Endothelial cells were treated with extracellular vesicles (EVs) derived from various cell types. Ki-67 staining was used to ...
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Effective Ultrafilter for Extracellular Vesicle/Exosome Concentration and Staining
July 28, 2023
Extracellular vesicles (EVs) isolated from cell culture media and resuspended in buffer were concentrated down to small volumes ...
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Cheap and Simple On-Column DNase Kit
July 28, 2023
Total RNA was isolated from lysed cells and extracellular vesicles for qPCR and miRNA sequencing. Column-based isolation was ...
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Handy Filter Sterilization of Culture Media From Millipore Sigma
July 26, 2023
Cell culture media prepared with non-sterile reagents can be filtered through the Steriflip unit for filter sterilization before ...
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Simple Centrifugal Filter Unit for Ultrafiltration of Culture Media From Millipore Sigma
July 26, 2023
Cells were cultured in monolayers for extracellular vesicle (EV) isolation. Centrifugal filter units (100kDa size cutoff) were ...
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Preferred Dissociated Reagent for Feeder-Free Stem Cell Culture
July 25, 2023
Both human iPSC and human ESC were cultured in feeder-free conditions on 1:100 matrigel-coated culture plates. ReLeSR reagent was ...
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Great, Reliable Mini Prep Kit from Zymo Research
April 05, 2023
We are interested in studying the mechanisms responsible for ensuring T cell self tolerance that rely on thyme and extra-thymic ...
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A Decent, Reliable Sanger Sequencing Service
March 27, 2023
We are interested in studying the mechanisms responsible for ensuring T cell self tolerance that rely on thyme and extra-thymic ...
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Great Service for Synthesizing Large DNA Fragments
March 23, 2023
We are interested in studying the mechanisms responsible for ensuring T cell self tolerance that rely on thyme and extra-thymic ...
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Great Post Aire Subpopulation Antibody
March 22, 2023
We are interested in studying the mechanisms responsible for ensuring T cell self tolerance that rely on thyme and extra-thymic ...
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High Quality Pre-Cast Western Blotting Gels
March 22, 2023
We are interested in studying the mechanisms responsible for ensuring T cell self tolerance that rely on thyme and extra-thymic ...
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Product Review
Excellent Astrocyte Labeling with Low Background in Mouse Hippocampus
Ksenia Novototskaya
(2)
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We used this antibody to immunofluorescently label astrocytes in the mouse dorsal hippocampus following astrocyte-specific MOR knockdown. The signal was bright,...
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