R&D Systems
Mouse TSPAN8 Alexa Fluor® 594-conjugated Antibody
FAB6524T-100UG
N/A
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We are interested in studying the mechanisms responsible for ensuring T cell self tolerance that rely on thyme and extra-thymic Aire expressing cells.
Flow Cytometry
Mouse thymus
Live dead: Zombie NIR LD 1:4000 in 100ul FACS for 20 min at RT
2.4G2
Surface stains: 1:100 in 100uL FACS for 30 min at 4C
PFA (15 min RT) + FoxP3 Fix/Perm kit (30 min 4C), then intracellular stain in 100uL perm buffer for 1hr at RT
Cytek Aurora spectral flow cytometry
This antibody give great detection of extremely rare post-Aire mTEC populations in the thymus by spectral flow cytometry. The manufacturer's recommended protocol worked well without further titration/optimization.
Good, easy to optimize staining.
None
Great antibody for studying thymic epithelial cell populations.