Elena Seranova

Graduate Student

University of Birmingham

Refrigerated Centrifuge from Eppendorf
November 11, 2019
Very good centrifuge we use routinely in the lab. Care needs to be taken in order to maintain it in good condition. It reaches the... read more
Excellent Antibody for Tubulin b3/Tuj1 from BioLegend
November 08, 2019
Would recommend for characterizing neuronal cultures. Can be combined with other assays and fluorescent labels. read more
Potent Human Recombinant Cytokine BMP-4
November 04, 2019
This BMP-4 human recombinant cytokine worked very well in our protocol and allowed the production of hematopoietic cells in ... read more
MAP2 Antibody of Good Quality (Sigma)
October 18, 2019
Would recommend this antibody as the staining worked well, with no background staining, and no other cells labeled other than ... read more
Dissociation Reagent for hPSCs
October 11, 2019
Stable at RT, reproducible results. read more
Dissociation Reagents for hPSCs
October 10, 2019
Working well with all stem cell lines we have in the lab, when cultured on feeder mouse embryonic fibroblasts (MEFs). Remains ... read more
Reliable Selection Antibiotic for Various Cell Lines
October 09, 2019
In stable cell lines, we perform a couple of passages adding Geneticin into the cultures to ensure the population remains ... read more
Good iNOS Antibody from Proteintech
September 27, 2019
Proteintech does usually have good antibodies but this one did not produce results as expected. Not sure whether to blame it on ... read more
Good Glycine Reagent from Sigma
September 25, 2019
Would recommend this product as its easy to use, stored in room temperature and can be stored over a long period of time. The ... read more
Great, Easy-to-Use PCR Master Mix
September 23, 2019
We were quite satisfied with this product and would definitely recommend! Once all DNA samples are diluted to the desired ... read more
Excellent Antibody for LC3B Detection by Immunoblotting from Novus
May 10, 2019
Would definitely recommend this antibody for western blotting. So far it has worked in all of the cell lines we tried it on in our... read more
Cytokine/Growth Factor for the Maintenance of Human Monocytes and Macrophages
May 09, 2019
Reagent of good quality. Cells present a healthy phenotype and functional characterization experiments confirm it (phagocytosis, ... read more
Excellent Kit for ADP/ATP Ratio Detection from BioVision
May 08, 2019
Very sensitive kit, does not need lots of cells to detect ATP and ADP. read more
Dead/Alive Staining Kit from Invitrogen
May 07, 2019
Very easy-to-use kit with just a drop or two needing to be added to the cells. read more
Sensitive Kit, Good Results
April 29, 2019
Good kit to use for detection of NAD and NADH (after denaturing the NAD sample). The plate needs to be set up in advance and ... read more

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Reliable and Reproducible MTT Assay for Cell Viability Studies

Gherardo Baudo

MTT was prepared at 5 mg/mL in PBS, and 20 µL was added to each well containing 200 µL of medium (220 µL total volume). Cells were incubated for 4 hours at 37°C... read more

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