Dead/Alive Staining Kit from Invitrogen

May 07, 2019

BlueskyReddit
University of Birmingham
ICGS
Graduate Student

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Company:

Invitrogen

Product Name:

Ready Probes Cell Viability imaging kit

Catalog Number:

R37610

My general research aim is to investigate the role of autophagy in neurodegenerative and infectious diseases, using stem cells and stem cell-derived human cellular platforms. The product is a kit for evaluating the percentage of dead and alive cells in a population via fluorescence imaging.

Experimental Design and Results Summary

Application

Cell death measurement in live cells

Starting Material

Cell lines of interest, fixative and mounting reagents

Tips

If fluorescent microscope is available, make sure the reagents already worked.

Results Summary

We imaged the cells after staining them with both reagents from the kit. The incubation time that was suggested by the manufacturer was not enough to stain the cells with Hoechst and therefore we needed to prolong the incubation to 30 minutes.

DOI or PMID #

N/A

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Summary

The Good

Very easy-to-use kit with just a drop or two needing to be added to the cells.

The Bad

15 minutes might not be enough to see good staining

The Bottom Line

If you don't have a fluorescent microscope in the lab, it might be a good idea to prolong the incubation (asked the manufacturer and they confirmed that incubation is in RT) up to 30 mins at least in 1 sample. In our lab, even after 30 mins some cells were not stained properly though.

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