Raghavendra Vadla

Western Blot of HeLa Whole-Cell Lysates Using Actin-Specific Antibody
February 20, 2019
Our aim was to examine the protein levels of our work under untreated and bleomycin (which induces double-stranded breaks) treated... read more
Western Blot of HeLa Cell Histone Proteins Treated with Phospho-Histone H2A.X (Ser139) Antibody
February 19, 2019
In response to strand breaks in the DNA, ATM phosphorylates H2AX on serine 139, which is required for DNA damage signaling and ... read more
Western Blot of TAP-SPT16 in S. cerevisiae Whole-Cell Lysates
February 18, 2019
SPT16 is a component of FACT complex (SPT16-Pob3) in Saccharomyces cerevisiae. FACT complex is involved in the regulation of ... read more
Western Blot Using H4K16 Acetylation-Specific Antibody
January 09, 2019
Our aim was to examine the levels of H4K16 acetylation in HeLa cells treated with an inhibitor of cell proliferation. We treated ... read more
Western Blot Using H3K9 Acetylation-Specific Antibody
December 27, 2018
Our aim was to examine the levels of H3K9 acetylation in HeLa cells treated with an inhibitor of cell growth. HeLa cells were ... read more
Sucrose Gradient Centrifugation of MNase-Digested Chromatin to Isolate Nucleosomes
December 21, 2018
The aim of our experiment is to perform sucrose gradient centrifugation to fractionate mononucleosomes from MNase-digested yeast ... read more
Western Blot of Purified H2AZ-FLAG Protein Using FLAG-Specific Antibody
November 21, 2018
Our aim was to study the interaction between H2AZ and other proteins. For that, we purified H2AZ-FLAG protein from S.cerevisiae ... read more
Western Blot Using Rictor-Specific Antibody
November 20, 2018
Our aim was to examine the levels of histone acetylation in rictor-knockdown cells. We transfected HeLa cells with the scramble ... read more
Western Blot Using Raptor-Specific Antibody
November 19, 2018
The aim of our experiment was to check the levels of raptor protein in whole-cell lysates of HeLa cells transfected with scramble ... read more
Western Blot of HeLa Whole Cell Lysates Using Hamartin/TSC1-Specific Antibody
October 10, 2018
The aim of our experiment was to study the role of TSC1/Hamartin in the regulation of mTOR signaling in HeLa cells. We used this ... read more
Western Blot of HeLa Whole Cell Lysates Using TSC2-Specific Antibody
October 08, 2018
The aim of our research was to study the role of Tuberin (TSC2) protein in regulating mTOR signaling in mammalian cells. We used ... read more
Excellent Antibody for Western Blot
October 03, 2018
The aim of our work is to check the levels of AKT phosphorylation at serine 473 (pAKT ser473) in TSC2-knockdown cells to ... read more
Excellent Antibody for Western Blot
October 01, 2018
We examined the levels of tubulin in HeLa whole cell lysates of control vs siRNA-mediated knockdown of protein X. read more
Western Blot of HeLa Cell Lysates Using H4 Specific Antibody
September 12, 2018
Our goal was to determine the levels of H4K16 acetylation in control vs protein X knockdown in whole cell lysates of HeLa cells. ... read more
Good Anti-Histone H2A.Z Antibody
September 11, 2018
Our goal was to study the stability of H2A in the nucleosomes. For that, we immunoprecipitated Flag-H2A nucleosomes from yeast ... read more

Articles by Raghavendra Vadla

Western Blot of HeLa Whole-Cell Lysates Using Actin-Specific Antibody

Western Blot of HeLa Cell Histone Proteins Treated with Phospho-Histone H2A.X (Ser139) Antibody

Western Blot of TAP-SPT16 in S. cerevisiae Whole-Cell Lysates

Western Blot Using H4K16 Acetylation-Specific Antibody

Western Blot Using H3K9 Acetylation-Specific Antibody

Sucrose Gradient Centrifugation of MNase-Digested Chromatin to Isolate Nucleosomes

Western Blot of Purified H2AZ-FLAG Protein Using FLAG-Specific Antibody

Western Blot Using Rictor-Specific Antibody

Western Blot Using Raptor-Specific Antibody

Western Blot of HeLa Whole Cell Lysates Using Hamartin/TSC1-Specific Antibody

Western Blot of HeLa Whole Cell Lysates Using TSC2-Specific Antibody

Excellent Antibody for Western Blot

Excellent Antibody for Western Blot

Western Blot of HeLa Cell Lysates Using H4 Specific Antibody

Good Anti-Histone H2A.Z Antibody

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