Western Blot of Purified H2AZ-FLAG Protein Using FLAG-Specific Antibody

November 21, 2018

Laboratory of t-RNA Transcription
Centre for Cellular and Molecular Biology
Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Sigma

Product Name:

ANTI-FLAG antibody

Catalog Number:

F7425

Our aim was to study the interaction between H2AZ and other proteins. For that, we purified H2AZ-FLAG protein from S.cerevisiae and examined the levels of H2AZ by using FLAG antibody. The antibody is excellent and gives a specific band corresponding to FLAG.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Saccharomyces cerevisiae cell lysate

Primary Incubation

1:5000, Overnight at 4°C

Blocking Agent

5% BSA

Secondary Incubation

1:10,000, anti-rabbit HRP conjugated antibody, 1 hr at room temperature

Tertiary Incubation

N/A

Detection

ECL reagent, Chemi doc

Results Summary

We observed a single band corresponding to H2AZ-FLAG protein by FLAG-specific antibody.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Excellent antibody for performing western blots

The Bad

N/A

The Bottom Line

An excellent antibody which gives a specific band corresponding to FLAG on western blot and easy to optimize. Used antibody can be reused multiple times.

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