Western Blot Using Raptor-Specific Antibody

November 19, 2018

Laboratory of t-RNA Transcription
Centre for Cellular and Molecular Biology
Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-Raptor antibody [EP539Y]

Catalog Number:

ab40768

The aim of our experiment was to check the levels of raptor protein in whole-cell lysates of HeLa cells transfected with scramble and raptor-specific siRNA. Samples were loaded on SDS-PAGE and transferred onto PVDF membrane. Raptor-specific antibody obtained from Abcam was successfully used to analyze the protein levels.

Experimental Design and Results Summary

Applications

Western Blot

Sample

HeLa whole-cell lysate

Primary Incubation

1:2000, Overnight at 4°C

Blocking Agent

5% skimmed milk, 1 hr at room temperature

Secondary Incubation

1:10000, anti-rabbit HRP conjugated antibody, 1 hr at room temperature

Tertiary Incubation

N/A

Detection

ECL reagent, Chemi doc

Results Summary

A specific band at 150 KD corresponding to raptor protein was obtained in scramble siRNA treated lysates and a reduction in protein levels were observed in raptor siRNA treated cell lysates.

DOI or PMID #

29143563

Additional Notes

N/A

Related Categories

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Summary

The Good

Good antibody for performing western blots

The Bad

N/A

The Bottom Line

Good antibody and easy to optimize for western blots

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