Excellent Antibody for Western Blot

October 01, 2018

BlueskyReddit
Laboratory of t-RNA Transcription
Centre for Cellular and Molecular Biology
Research Associate

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Cell Signaling Technology

Product Name:

α-Tubulin (11H10) Rabbit mAb

Catalog Number:

2125

We examined the levels of tubulin in HeLa whole cell lysates of control vs siRNA-mediated knockdown of protein X.

Experimental Design and Results Summary

Applications

Western Blot

Sample

HeLa whole cell lysate

Primary Incubation

1:5000, Overnight at 4°c

Blocking Agent

5% skimmed milk, 1 hr at room temperature

Secondary Incubation

1:10000, anti-rabbit HRP conjugated antibody, 1 hr at room temperature

Tertiary Incubation

N/A

Detection

ECL reagent, Chemi doc

Results Summary

Good antibody for western blot and detects a specific band at 52KD. This antibody doesn't give non-specific bands.

DOI or PMID #

29143563

Additional Notes

N/A

Image Gallery

Summary

The Good

Specific band at 52KD

The Bad

N/A

The Bottom Line

Excellent antibody for western blot

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