Claire Stewart

Graduate Student

Colorado State University

Antibody User Review
ATF4 To Detect The Presence Of ER Stress
September 12, 2017
PERK is a key protein that is phosphorylated and activated during ER stress, responsible for the phosphorylation eIF2alpha which ... read more
Antibody User Review
CD147 As A Measure Of ERAD
August 17, 2017
CD147 has been known to be a substrate of endoplasmic reticulum associated degradation (ERAD). The objective of this study was to ... read more
Assay Kit/Reagent User Review
Proximity Ligation Assay To Detect Protein Interactions
February 06, 2017
The purpose of this experiment was to test and quantify mitochondrial associated membrane (MAM) formation using two proteins that ... read more
Antibody User Review
Immunofluorescence Of IP3R For Proximity Ligation Assay
January 31, 2017
The mitochondrial associated membrane (MAM) is a well established point of communication between the ER and mitochondria. IP3R is ... read more
Antibody User Review
VDAC For Proximity Ligation Assay
January 30, 2017
The mitochondrial associated membrane (MAM) is now an established point of communication between the ER and mitochondria. VDAC is ... read more
Equipment User Review
DP73 Digital Camera From Olympus
January 24, 2017
This camera has been used in conjunction with the Olympus BX63 microscope to assess fixed bright field as well as fluorescent ... read more
Antibody User Review
Beta Actin As A Western Blot Loading Control
January 09, 2017
Beta actin was used as a loading control for western samples to assure that protein loading is the same across the gel. This is ... read more
Antibody User Review
Phosphorylated eIF2a to Detect ER Stress
January 06, 2017
eIF2a is a well known substrate of at least 4 kinases, the most well known being PERK. When, phosphorylated, it has been ... read more
Antibody User Review
Ubiquitin Antibody For Detection Of Poly-Ubiquitinated Proteins
December 14, 2016
The ubiquitin proteasome system is critical for maintaining cellular health and defects in the system can result in pathogenesis ... read more
Assay Kit/Reagent User Review
Proteasome Function In Mammalian Cells
November 22, 2016
The goal of this experiment was to determine the activity level of the proteasome in H4IIE rat hepatoma cells due to a variety of ... read more
Assay Kit/Reagent User Review
Pierce BCA Protein Assay Kit For Quantitative Total Protein
November 14, 2016
One of the most basic biochemical tools is the western blot and to be able to perform a reliable western blot, reliable protein ... read more
Antibody User Review
IP3R Immunofluorescence
November 08, 2016
The interaction between the ER and mitochondria has been shown to be involved in the development and progression of obesity and ... read more
Antibody User Review
VDAC Fluorescent Microscopy
November 07, 2016
The interaction and communication between the ER and mitochondria has been shown to be involved in development and progression of ... read more
Antibody User Review
P58 Antibody from Abcam
August 30, 2016
This protein is involved in the unfolded protein response and may inhibit eIF2alpha phosphorylation and therefore PERK activity. ... read more
Antibody User Review
Total PERK
August 29, 2016
PERK is a key protein that is phosphorylated and activated during ER stress, responsible for the phosphorylation eIF2alpha. ... read more

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Parijat Sarkar

I study the transcription of endogenous genes regulated by transcription factors, and it's crucial to quantify their expression levels accurately. To achieve ... read more

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