Abcam
Anti-EIF2S1
ab32157
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Browse Similar Products: EIF2S1 Western Blot Antibodies
eIF2a is a well known substrate of at least 4 kinases, the most well known being PERK. When, phosphorylated, it has been demonstrated to reduce global protein translation, a critical step in reversing ER stress. Our goal was to use this antibody for western blot to determine the amount of phosphorylated eIF2a in a variety of samples treated with various ER stress inducers.
Western Blot
Total cell lysates of H4IIE rat hepatoma cells
1:500 antibody in 1:3 Odyssey Block: TBST overnight, at 4 degrees C.
1:3 Odyssey Block: TBS for one hour at room temperature
1:15000 IRDye800CW in 1:3 Odyssey Block: TBST
N/A
Licor Odyssey Clx
This antibody works well and shows up at around 36kDa. There is an obvious difference between the treatments. These were compared to total eIF2a.
Minimal background, and strong antibody signal using abcam's dilution recommendation
N/A.
Order this antibody if you want clear, distinct p-eIF2a results with minimal background