Santa Cruz Biotechnology
IP3R-1 Antibody (E-8)
sc-271197
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Browse Similar Products: IP3RI Immunofluorescence Antibodies
The interaction between the ER and mitochondria has been shown to be involved in the development and progression of obesity and obesity-related disorders such as non-alcoholic fatty liver disease. IP3R is a protein associated with the ER and has been shown to interact with proteins of the mitochondria. This experiment was performed to identify IP3R using immunofluorescence in liver cells.
Immunofluorescence
H4IIE rat heptoma cells
1:50 antibody in 1% BSA in PBST for 1 hr. at room temperature
1% BSA, 22.52 mg/mL glycine in PBST (PBS+ 0.1% Tween 20) for 30 min at room temperature
1:200 Alexa Fluor dye 555 in 1% BSA in PBST for 1 hr. at room temperature in the dark
N/A
Olympus BX63 with Cy3 filter
IP3R seems to appear in the nucleus. It still appears outside of the nucleus but in considerably lower quantities. Not sure if this antibody is interacting with the DAPI nuclear stain or if too much antibody was used. Will have to continue to optimize or get a different antibody from somewhere else because this is not ideal and this protein should not be present in the nucleus.
None
Antibody is easily viewable
A lot of non-specific binding to nuclear area and/or interacts with DAPI.
Consider increased experiments for optimization or choose an antibody from a different company.