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Author Profile
Rebecca Wilshusen
Graduate Research Assistant
University of Nebraska Medical Center
Articles by Rebecca Wilshusen
Antibody User Review
Phenotyping CD4+ Effector T Cells With CD196 Antibody
March 01, 2018
This CD196-eFlour 660 antibody was used to investigate the phenotype of CD4+ effector T cells after enrichment from the spleen and...
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Antibody User Review
Use Of Mouse IgG1 Kappa Isotype Control, EFluor 660 To Control For Background Signal
February 23, 2018
In this experiment polarized CD4+ T cells were stained with an eFluor 660 primary antibody as well as the isotype for that ...
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Antibody User Review
Use Of Isotype Rat IgG1 Kappa Isotype Control in Flow Analysis
February 22, 2018
In this experiment control cells were treated with permeabilization buffer and labeled with isotype antibody in order to ...
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Antibody User Review
Phenotyping Polarized T Cells Using An Antibody Against The FoxP3 Transcription Factor
February 21, 2018
In this study T cells were isolated and polarized to a Th17 phenotype before testing for the expression of intracellular ...
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Antibody User Review
Characterizing Polarized T Cells Using T-Bet Monoclonal Antibody
February 20, 2018
T cells were isolated from C57Bl/6 wildtype mice and polarized for 5 days before testing the phenotype via T-bet Monoclonal ...
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Antibody User Review
Use Of ROR Gamma (T) Monoclonal Antibody To Characterize T Cell Polarizations
February 16, 2018
T cells were isolated from mice and polarized to a Th17 phenotype. This phenotype was then tested via intracellular staining for ...
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Antibody User Review
IgG2b Kappa Isotype Control
February 13, 2018
Microglial cells from the brains of 1-3 day old pups were isolated and cultured for 7 days in the presence of M-CSF to enrich for ...
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Antibody User Review
Purity Check Of Primary Microglial Cells Utilizing The CD45 Monoclonal Antibody
February 12, 2018
Primary microglial cells were isolated from the brains of 1-3 day old pups after 7 days in culture with M-CSF. The purity of the ...
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Assay Kit/Reagent User Review
Compensation Control In Flow Cytometric Analysis
February 07, 2018
The OneComp eBeads™ Compensation Beads were used as a single-color compensation controls for the BD LSR II instrument when running...
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Antibody User Review
Use Of CD11b Monoclonal Antibody (M1/70), PE To Purity Check Primary Microglial Cultures
February 06, 2018
Single cell suspensions were isolated from brain tissue of day 1-3 C57Bl/6 pups, incubated for 7 days with human M-CSF to enrich ...
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Antibody User Review
Use Of TNF alpha Monoclonal Antibody (MP6-XT22), EFluor 450 To Phenotype Polarized CD4+ T Cells
February 05, 2018
T cells were isolated from C57Bl/6 wild type mice and polarized to a Th17 phenotype using cytokines and antibodies and verified ...
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Assay Kit/Reagent User Review
Using Brefeldin A Solution (1000X) To Study Intracellular Cytokine Production
January 30, 2018
Cells were incubated for 5 hours in a stimulation medium consisting on PMA and Ionomycin in order to stimulate cytokine production...
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Antibody User Review
Alexa Fluor® 647 Anti-Mouse IL-17A Antibody To Phenotype CD4+ Th17 Cells After Polarization
January 29, 2018
CD4+ T cells were extracted from the spleens and lymph nodes of mice, selected for CD4 using a negative selection column, and then...
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Antibody User Review
Armenian Hamster IgG Isotype Control, PE In Flow Cytometry To Control For Background Signal To Noise Ratio
January 26, 2018
In this experiment teh Armenian Hamster IgG Isotype Control, PE was used in order to control for non-specific background signal ...
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Antibody User Review
Controling For Background Signal In Flow Cytometry Utilizing APC-H7 Rat IgG2b, kappa Isotype Control
January 19, 2018
In this experiment T cells isolated from the spleen and negatively selected for CD4+ using R&D columns were incubated with ...
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Efficient and Cost Friendly SYBR Green Mastermix for qPCR
Parijat Sarkar
I study the transcription of endogenous genes regulated by transcription factors, and it's crucial to quantify their expression levels accurately. To achieve ...
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