Alexa Fluor® 647 Anti-Mouse IL-17A Antibody To Phenotype CD4+ Th17 Cells After Polarization

January 29, 2018

Pharmacology and Experimental Neuroscience
University of Nebraska Medical Center
Graduate Research Assistant

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Biolegend

Product Name:

Alexa Fluor® 647 anti-mouse IL-17A Antibody

Catalog Number:

506911

CD4+ T cells were extracted from the spleens and lymph nodes of mice, selected for CD4 using a negative selection column, and then incubated for 5 days with cytokines and antibodies to skew the cells towards a TH17 phenotype before being tested for IL-17 production via flow cytometric analysis.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

CD3+/CD4+ T cell from mouse

Primary Incubation

0.1ug/1.0x105 cells for 1 hour

Blocking Agent

20ug/ml gamma globulin

Secondary Incubation

n/a

Tertiary Incubation

n/a

Detection

BD LSRII Flow Cytometer

Results Summary

This antibody produces phenomenal detection of intracellular IL-17 after permeabilization with little to no background signal detected.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

This antibody is inexpensive

The Bad

No drawbacks seen

The Bottom Line

This Biolegend antibody is great to use to stud intracellular cytokines produced in small amounts, such as IL-17a

Share your experience with other scientists. Write a Review! »

Join the discussion