Purity Check Of Primary Microglial Cells Utilizing The CD45 Monoclonal Antibody

February 12, 2018

Pharmacology and Experimental Neuroscience
University of Nebraska Medical Center
Graduate Research Assistant

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Company:

Invitrogen Antibodies

Product Name:

CD45 Monoclonal Antibody (30-F11), APC

Catalog Number:

17-0451-82

Primary microglial cells were isolated from the brains of 1-3 day old pups after 7 days in culture with M-CSF. The purity of the microglial culture was checked using flow cytometry.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Enriched primary microglia from mouse brain cells

Primary Incubation

0.1ug/1.0x105 cells for 30 minutes at 4 degrees celcius

Blocking Agent

Gamma globulin

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

BD LSRII Flow Cytometer

Results Summary

The CD45 Monoclonal Antibody (30-F11), APC antibody shows high affinity for CD45 with little to no background signal present.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

This antibody works well and is relatively inexpensive

The Bad

N/A

The Bottom Line

This antibody is an inexpensive option to use when looking to investigate extracellular CD45 expression.

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