Use Of TNF alpha Monoclonal Antibody (MP6-XT22), EFluor 450 To Phenotype Polarized CD4+ T Cells

February 05, 2018

Pharmacology and Experimental Neuroscience
University of Nebraska Medical Center
Graduate Research Assistant

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Company:

Invitrogen Antibodies

Product Name:

TNF alpha Monoclonal Antibody (MP6-XT22), eFluor 450

Catalog Number:

48-7321-82

T cells were isolated from C57Bl/6 wild type mice and polarized to a Th17 phenotype using cytokines and antibodies and verified via flow cytometric analysis for intracellular TNF alpha production.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

CD4+ T cells from mouse

Primary Incubation

0.1ug/1.0x105 cells for 1 hour at 4 degrees celcius

Blocking Agent

20ug/ml gamma globulin

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

BD LSRII Flow Cytometer

Results Summary

This intracellular antibody demonstrated consistent specific antibody signal over many experiments with little non-specific background signal.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

This antibody in consistent and has little bleed over from other channels.

The Bad

This antibody is relatively expensive

The Bottom Line

While being on the expensive side, this intracellular antibody produces reliable, consistent data over a long period of time.

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