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Author Profile
John Corthell
Graduate Student
Florida State University
Articles by John Corthell
Antibody User Review
Good antibody for immunohistochemistry and immunoblotting
February 23, 2015
I used this antibody for immunoblotting and immunohistochemistry on brain tissue samples. The antibody worked well for both ...
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Equipment User Review
Great imager with good support
February 19, 2015
This imager works well for fluorescent immunoblotting. The software was more user-friendly than a lot of the software available ...
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Antibody User Review
Anti-Connexin43 Antibody Works Well for Chemiluminescence
February 18, 2015
I wanted to examine connexin43 phosphorylation levels via immunoblot in brain tissue. This antibody worked well for ...
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Antibody User Review
GAD67 Antibody Works Well for Immunohistochemistry
February 12, 2015
I wanted to determine GAD67 location in fixed brain tissue. This antibody had been left in the refrigerator for years before I ...
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Antibody User Review
Great immunoblotting antibody
February 12, 2015
I wanted to quantify GluR4 protein expression in brain tissue homogenates. I initially tried the rabbit polyclonal anti-GluR4 ...
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Equipment User Review
Good confocal microscope, good software
February 09, 2015
I wanted to use confocal microscopy to examine brain tissue for different antigens. The software is cumbersome but useful once ...
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Antibody User Review
Great anti-parvalbumin immunohistochemistry antibody
February 06, 2015
In these experiments, I wanted to investigate whether a novel receptor (for olfactory bulb tissue) was co-expressed with known ...
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Antibody User Review
Great antibody for immunohistochemistry, lasts a long time
February 04, 2015
I wanted to detect calretinin in fixed brain tissue via immunohistochemistry. I pulled this antibody out of a -20 degrees C ...
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Antibody User Review
Anti-Connexin45, noisy but effective
February 03, 2015
In these experiments, I wanted to investigate whether a novel receptor (for olfactory bulb tissue) was co-expressed with known ...
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Antibody User Review
Great loading control antibody
February 03, 2015
I wanted to investigate whether the target proteins had any diurnal variations in olfactory bulb tissue and investigated whether ...
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Antibody User Review
Good anti-Connexin43 antibody for immunoblotting
January 29, 2015
In these experiments, I wanted to investigate whether a novel receptor (for olfactory bulb tissue) was co-expressed with known ...
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Assay Kit/Reagent User Review
Vectashield with DAPI does exactly what you want, no fuss
January 12, 2015
You drip a bit of this onto your tissue on the slide, coverslip, and you will have strong DAPI staining with low aberrant staining...
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Equipment User Review
Solid workhorse machine
October 07, 2014
We wanted to perform qPCR testing to determine if treatment of a G-protein-coupled receptor with an agonist would affect the ...
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Assay Kit/Reagent User Review
Kit for isolation of DNA, total RNA and protein
August 21, 2014
This kit was used to collect DNA, RNA, and protein for PCR, qPCR, and Western blotting for a circadian rhythms experiment. We ...
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Assay Kit/Reagent User Review
Great Kit for RNA Extraction
August 06, 2014
Extraction of total RNA from brain tissue for use in qPCR experiments. Selected this product in order to purify RNA extracted ...
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Efficient and Cost Friendly SYBR Green Mastermix for qPCR
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I study the transcription of endogenous genes regulated by transcription factors, and it's crucial to quantify their expression levels accurately. To achieve ...
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