John Corthell

Graduate Student

Florida State University

Good antibody for immunohistochemistry and immunoblotting
February 23, 2015
I used this antibody for immunoblotting and immunohistochemistry on brain tissue samples. The antibody worked well for both ... read more
Great imager with good support
February 19, 2015
This imager works well for fluorescent immunoblotting. The software was more user-friendly than a lot of the software available ... read more
Anti-Connexin43 Antibody Works Well for Chemiluminescence
February 18, 2015
I wanted to examine connexin43 phosphorylation levels via immunoblot in brain tissue. This antibody worked well for ... read more
GAD67 Antibody Works Well for Immunohistochemistry
February 12, 2015
I wanted to determine GAD67 location in fixed brain tissue. This antibody had been left in the refrigerator for years before I ... read more
Great immunoblotting antibody
February 12, 2015
I wanted to quantify GluR4 protein expression in brain tissue homogenates. I initially tried the rabbit polyclonal anti-GluR4 ... read more
Good confocal microscope, good software
February 09, 2015
I wanted to use confocal microscopy to examine brain tissue for different antigens. The software is cumbersome but useful once ... read more
Great anti-parvalbumin immunohistochemistry antibody
February 06, 2015
In these experiments, I wanted to investigate whether a novel receptor (for olfactory bulb tissue) was co-expressed with known ... read more
Great antibody for immunohistochemistry, lasts a long time
February 04, 2015
I wanted to detect calretinin in fixed brain tissue via immunohistochemistry. I pulled this antibody out of a -20 degrees C ... read more
Anti-Connexin45, noisy but effective
February 03, 2015
In these experiments, I wanted to investigate whether a novel receptor (for olfactory bulb tissue) was co-expressed with known ... read more
Great loading control antibody
February 03, 2015
I wanted to investigate whether the target proteins had any diurnal variations in olfactory bulb tissue and investigated whether ... read more
Good anti-Connexin43 antibody for immunoblotting
January 29, 2015
In these experiments, I wanted to investigate whether a novel receptor (for olfactory bulb tissue) was co-expressed with known ... read more
Vectashield with DAPI does exactly what you want, no fuss
January 12, 2015
You drip a bit of this onto your tissue on the slide, coverslip, and you will have strong DAPI staining with low aberrant staining... read more
Great primers at good prices
October 09, 2014
I have had a great experience ordering primers from IDT. They have always given me high-quality primers, shipped very quickly, ... read more
Solid workhorse machine
October 07, 2014
We wanted to perform qPCR testing to determine if treatment of a G-protein-coupled receptor with an agonist would affect the ... read more
Kit for isolation of DNA, total RNA and protein
August 21, 2014
This kit was used to collect DNA, RNA, and protein for PCR, qPCR, and Western blotting for a circadian rhythms experiment. We ... read more

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Excellent Astrocyte Labeling with Low Background in Mouse Hippocampus

Ksenia Novototskaya

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We used this antibody to immunofluorescently label astrocytes in the mouse dorsal hippocampus following astrocyte-specific MOR knockdown. The signal was bright,... read more

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