Seth Tompkins

Research Intern

Penn State College of Medicine

Great FTH Antibody for Western Blotting
May 11, 2023
I used this antibody to indirectly measure intracellular iron stores. When cells take up iron, it can enter the labile iron pool ... read more
Great FTL Antibody for Western Blotting
May 11, 2023
I used this antibody to indirectly iron storage. Once in the cell, iron is stored in ferritin which is made up of h-ferritin and l... read more
Great IRP1 Antibody for Western Blotting
May 10, 2023
I used this antibody to indirectly measure the labile iron pool. When intracellular iron increases, IRP1 is degraded. read more
CD10 Polyclonal Antibody from ProteinTech Works Fine
May 01, 2023
I used this to measure the levels of CD10. This protein is also known by NEP-1 and is an Amyloid-beta degrading enzyme. I used ... read more
Good IRP2 Antibody to Measure Labile Iron Pool
May 01, 2023
I used this as a marker for the labile iron pool within cells. Cellular iron levels modulate IRP2 and IRP1 levels - though usually... read more
Good APP Antibody for Western Blot
April 28, 2023
I used this to determine if my treatment changed APP levels. APP is the precursor to amyloid beta and plays a vital role in iron ... read more
Good Actin Antibody for Western Blot Control
April 27, 2023
I used this as a loading control for western blots. It worked very well since I was looking at proteins of higher and lower ... read more
Good Mitochondrial Marker from Proteintech
April 27, 2023
I used this to detect mitochondria in a sample. TOM20 is a protein on the outer membrane of mitochondria. It is a marker of ... read more
Key DMEM/F-12 Medium in iPSC Culture
April 26, 2023
This medium is used for both the coating of Matrigel plates and the differentiation of induced pluripotent stem cells into ... read more
Anti-Ferritin Heavy Chain Antibody Did Not Work for Me
April 26, 2023
Ferritin is an iron storage protein. It is often used as a marker of cellular iron content. This antibody however did not work in ... read more
Excellent Human Endothelial SFM for Endothelial Cell Culture
April 25, 2023
This medium is used to maintain endothelial cells. Specifically, it is used in the protocol to differentiate endothelial cells ... read more
Good Medium for iPSC Differnetiation
April 24, 2023
This medium is used in the differentiation of induced pluripotent stem cells to endothelial cells. The medium is used to prime the... read more
TeSR E5/E6 Medium Works Well for iPSC Differentiation
April 24, 2023
This medium is used in the differentiation of induced pluripotent stem cells to endothelial cells. The medium is used to prime the... read more
Great Cytochrome C Antibody for Western Blotting
April 24, 2023
Cytochrome C is an iron rich complex in mitochondria. This antibody works beautifully to detect the small protein. read more
Good Essential 8™ Medium for iPSC Culture
April 21, 2023
This media is used for induced pluripotent stem cell maintenance and culture. I was this medium for that exact purpose. read more

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Excellent Astrocyte Labeling with Low Background in Mouse Hippocampus

Ksenia Novototskaya

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We used this antibody to immunofluorescently label astrocytes in the mouse dorsal hippocampus following astrocyte-specific MOR knockdown. The signal was bright,... read more

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