Seth Tompkins

Research Intern

Penn State College of Medicine

Antibody User Review
Great FTH Antibody for Western Blotting
May 11, 2023
I used this antibody to indirectly measure intracellular iron stores. When cells take up iron, it can enter the labile iron pool ... read more
Antibody User Review
Great FTL Antibody for Western Blotting
May 11, 2023
I used this antibody to indirectly iron storage. Once in the cell, iron is stored in ferritin which is made up of h-ferritin and l... read more
Antibody User Review
Great IRP1 Antibody for Western Blotting
May 10, 2023
I used this antibody to indirectly measure the labile iron pool. When intracellular iron increases, IRP1 is degraded. read more
Antibody User Review
CD10 Polyclonal Antibody from ProteinTech Works Fine
May 01, 2023
I used this to measure the levels of CD10. This protein is also known by NEP-1 and is an Amyloid-beta degrading enzyme. I used ... read more
Antibody User Review
Good IRP2 Antibody to Measure Labile Iron Pool
May 01, 2023
I used this as a marker for the labile iron pool within cells. Cellular iron levels modulate IRP2 and IRP1 levels - though usually... read more
Antibody User Review
Good APP Antibody for Western Blot
April 28, 2023
I used this to determine if my treatment changed APP levels. APP is the precursor to amyloid beta and plays a vital role in iron ... read more
Antibody User Review
Good Actin Antibody for Western Blot Control
April 27, 2023
I used this as a loading control for western blots. It worked very well since I was looking at proteins of higher and lower ... read more
Antibody User Review
Good Mitochondrial Marker from Proteintech
April 27, 2023
I used this to detect mitochondria in a sample. TOM20 is a protein on the outer membrane of mitochondria. It is a marker of ... read more
Assay Kit/Reagent User Review
Key DMEM/F-12 Medium in iPSC Culture
April 26, 2023
This medium is used for both the coating of Matrigel plates and the differentiation of induced pluripotent stem cells into ... read more
Antibody User Review
Anti-Ferritin Heavy Chain Antibody Did Not Work for Me
April 26, 2023
Ferritin is an iron storage protein. It is often used as a marker of cellular iron content. This antibody however did not work in ... read more
Assay Kit/Reagent User Review
Excellent Human Endothelial SFM for Endothelial Cell Culture
April 25, 2023
This medium is used to maintain endothelial cells. Specifically, it is used in the protocol to differentiate endothelial cells ... read more
Assay Kit/Reagent User Review
Good Medium for iPSC Differnetiation
April 24, 2023
This medium is used in the differentiation of induced pluripotent stem cells to endothelial cells. The medium is used to prime the... read more
Assay Kit/Reagent User Review
TeSR E5/E6 Medium Works Well for iPSC Differentiation
April 24, 2023
This medium is used in the differentiation of induced pluripotent stem cells to endothelial cells. The medium is used to prime the... read more
Antibody User Review
Great Cytochrome C Antibody for Western Blotting
April 24, 2023
Cytochrome C is an iron rich complex in mitochondria. This antibody works beautifully to detect the small protein. read more
Assay Kit/Reagent User Review
Good Essential 8™ Medium for iPSC Culture
April 21, 2023
This media is used for induced pluripotent stem cell maintenance and culture. I was this medium for that exact purpose. read more

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Flow Staining of In Vitro Translation Activity of GM-CSF mRNA Vaccine

Haoyu Sun

(1)
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This is used for in vitro translation activity detection. Cells transfected for 24h were collected and the samples were stained with this antibody, and then a ... read more

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