Anti-Ferritin Heavy Chain Antibody Did Not Work for Me

April 26, 2023

Neurosurgery
Penn State College of Medicine
Research Intern

Overall

Quality of Results

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Company:

Abcam

Product Name:

Ferritin antibody

Catalog Number:

ab77127

Clone Number:

N/A

Ferritin is an iron storage protein. It is often used as a marker of cellular iron content. This antibody however did not work in my cells at all.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human astrocytes

Primary Incubation

1:1000 overnight BSA

Blocking Agent

BSA

Secondary Incubation

1 hr, milk

Tertiary Incubation

N/A

Detection

ECL

Results Summary

I tried to use this to determine if, in astrocyte, my treatment increased iron content of the cells. The antibody however, did not work.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Nothing

The Bad

Did not work for me.

The Bottom Line

I do not recommend it.

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