Cell Signaling
IRP2
37135S
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I used this as a marker for the labile iron pool within cells. Cellular iron levels modulate IRP2 and IRP1 levels - though usually only one so it is important to check both. Decreased iron leads to the stabilization of these proteins, while increased iron leads to the degradation.
Western Blot
human astrocytes
1:1000 overnight BSA
BSA
1 hr, milk
None
ECL
This allowed me to show that my treatment did not change IRP2 levels. I did check IRP1 and saw changes, emphasizing the importance of checking both proteins.
Works well
Nothing
I recommend