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Author Profile
Bethany Cragoe
Babraham Institute
Articles by Bethany Cragoe
Highly Recommended Antibody From Cell Signaling for Western Blot
October 12, 2023
Clean blots, sensitivity is good, great for blotting for tubulin as a loading control. They are used in MCF10a cells.
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Antibody IP'd PANK4 From Bethyl Laboratories
October 04, 2023
Antibody successfully IP'd PANK4 from MCF10a cells, but quite a messy blot and needs a lot of starting material.
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Great Antibody From Cell Signalling for Western Blot
October 04, 2023
Very clean blot from MCF10a cells, and easy to optimise. Would definitely buy again to detect pMTORC2.
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Great Anti-Succinyllysine Antibody for Western Blotting
June 12, 2023
Great antibody for detecting overall succinyllysine levels in MCF10a cells. A lot of these antibodies used to detect overall ...
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Great OGDH Antibody for Western Blotting
June 07, 2023
A great antibody for detecting levels of OGDH in MCF10a cells. Very clear bands with no background noise.
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Great PDPK1(Tyr373 + Tyr376) Antibody for Western Blotting
May 05, 2023
Good antibody for western blotting - very little background noise, clear signal. Used BSA to block and added 1:1000 dilution of ...
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Great ZNF518B Antibody for Western Blotting
May 03, 2023
Good antibody for detecting ZNF518b in MCF10a cells. Little background noise and can reuse multiple times.
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Good GPR143 Antibody but Some Background Noise
May 02, 2023
Good antibody - tried first time to check GPR143 levels in MCF10a cells. A lot of background came up with 1:500 dilution but you ...
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Great GAMT Monoclonal Antibody for Western Blot
March 29, 2023
Great antibody for western blotting. There were a few non specific bands but used for the first time and could clearly see the ...
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Great Anti-Histone H3 Antibody, Strong Signal
February 27, 2023
Great antibody for western blotting. Would recommend diluting slightly (1:2000-5000) as very strong signal.
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Great Anti-acetyl-Histone H3 Antibody for Western Blot
February 24, 2023
Very good antibody - clean bands with little background. Easy to use. One of the best I have used for acetylated-H3.
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Great Phospho-ATP-Citrate Lyase Antibody, Very Clean Bands
February 23, 2023
Used this antibody to check pACLY downstream of EGF stimulation. Clear bands with little background.
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Great Hoechst 33342 Solution for Live Cell Staining
January 23, 2023
Used this product at a final concentration of 1mg/ml, to stain the nuclei of living cells during imaging.
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Great Phospho-p44/42 MAPK Antibody for Western Blotting
January 20, 2023
Used this antibody as a MAPK signalling downstream of the EGF receptor in MCF10a cells. The antibody has very high signal so use ...
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Great p44/42 MAPK (Erk1/2) Antibody for Western Blotting
December 15, 2022
Very strong antibody for western blotting total Erk in MCF10a cell lysates. Strong signal so only small amount of antibody needed.
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