Good SirT3 Antibody from Cell Signaling

May 24, 2022

NanoEngineering
UC San Diego NanoEngineering Department
Graduate Student

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Company:

Cell Signaling

Product Name:

SirT3 (D22A3) Rabbit mAb

Catalog Number:

5490S

Clone Number:

N/A

Info:Supplier Page View Product Specs

Browse Similar Products: SIRT3 Antibodies

I seed epithelial cells (lung cancer cells) for 24 hours and I treated with eIF4E siRNA for 24 hours. I evaluated SirT3 expression with western blot diluting it up to 1:1000 and incubating the membrane overnight at 4 °C.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Epithelial cells

Primary Incubation

1:1000, 4 °C, overnight

Blocking Agent

Milk 5% TBST

Secondary Incubation

1:5000

Tertiary Incubation

N/A

Detection

Chemiluminescence

Results Summary

I seed epithelial cells (lung cancer cells) for 24 hours and I treated with eIF4E siRNA for 24 hours. I evaluated SirT3 expression with western blot diluting it up to 1:1000 and incubating the membrane overnight at 4 °C.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

I was able to get some results

The Bad

Band isn't so clear

The Bottom Line

Expensive but great, would buy again

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