Good Phospho-mTOR Antibody with Optimization

May 23, 2022

BlueskyReddit
NanoEngineering
UC San Diego NanoEngineering Department
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling

Product Name:

Phospho-mTOR (Ser2448) Antibody

Catalog Number:

2971

Clone Number:

N/A

I seeded epithelial cells with 10% FBS. The day after I replaced the medium (starvation medium, 0.5%) and I treated with eIF4E siRNA for 24 hours. pmTOR has a really high molecular weight, so I had difficulties detecting it.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Epithelial cells

Primary Incubation

1:1000, 4 °C, overnight

Blocking Agent

BSA 5% TBST

Secondary Incubation

1:5000

Tertiary Incubation

N/A

Detection

Chemiluminescence

Results Summary

I seeded epithelial cells with 10% FBS. The day after I replaced the medium (starvation medium, 0.5%) and I treated with eIF4E siRNA for 24 hours. pmTOR has a really high molecular weight so I had difficulties detecting it.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Band detectable.

The Bad

Some unspecific bands.

The Bottom Line

Antibody works, but you need to optimize your conditions.

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