Great HIF-1a Antibody for Western Blot

May 23, 2022

BlueskyReddit
NanoEngineering
UC San Diego NanoEngineering Department
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling

Product Name:

HIF-1α (D2U3T) Rabbit mAb

Catalog Number:

14179

Clone Number:

N/A

I exposed my endothelial cells for 24 hours in hypoxia chamber (O = 1% and CO2 = 5%). I diluted the primary antibody up to 1:1000 and I got great results, level of hif1a was increased.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Endothelial cells

Primary Incubation

1:1000, 4 °C, overnight

Blocking Agent

BSA 5% TBST

Secondary Incubation

1:10000

Tertiary Incubation

N/A

Detection

Chemiluminescence

Results Summary

I exposed my endothelial cells for 24 hours in hypoxia chamber (O = 1% and CO2 = 5%). I diluted primary antibody up to 1:1000 and I got great results, level of hif1a was increased.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Band is clear.

The Bad

N/A

The Bottom Line

Antibody has great signal, highly recommended.

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