ID7000™ Spectral Cell Analyzer from Sony Corporation

Product Specs
ItemID7000™ Spectral Cell Analyzer
CompanySony Corporation
Price Inquire
Catalog NumberID7000
Detection32-channels (PMT arrays); can be configured with 186 detectors to detect 44 colors or more
Throughput40,000 events per second
Lasers3 to 7 lasers
Excitation320 nm, 355 nm, 405 nm, 488 nm, 561 nm, 637 nm, 808 nm
Parameters186 fluorescence channels
CapacityTubes (5 mL), Microplates using autosampler (96-well, 384-well)
Data Throughput40,000 events/sec
Dimensions(WxDxH) 106 x 71.9 x 76 cm
Flow Rate33 to 200 µL/min

(81) Piezo1-Fstl1 Axis in Fracture Healing: Modulation of the Chondrocyte Inflammation-ROS-Mitochondrial Damage Cascade and Application of Smart Delivery SystemInternational Journal of Biological SciencesFebruary 18, 2026Tao Zhang, Haoran Wang, Guangzhao Hou, Siming Jia, Kai Ding, Qian Xiao, Jinbo Liu, Yajiang Dai, Gang Lv, Zhiyong Hou, et al.fluorescence was detected by fluorescence microscope (Olympus) and flow cytometry respectively (Sony ID7000). Mitochondrial fluorescent staining (JC-1, Mito-Tracker, MitoSOX and mPTP) Details of the method

(82) Phenotype of circulating tumor-reactive T cells predicts immune checkpoint inhibitor response in non-small cell lung cancerNature CommunicationsFebruary 17, 2026Katsuhiro Ito, Kei Iida, Tomoko Hirano, Merrin Man Long Leong, Kenji Morii, Toshi Menju, Hiroshi Date, Hiroaki Ozasa, Hironori Yoshida, Toyohiro Hirai, et al.0.5%7-AAD (TOMBO Biosciences), 0.2% Stained samples were washed twice with 2% FCS/PBS and analyzed using an ID7000 spectral flow cytometer (Sony Biotechnology). Human samples Cryopreserved PBMCs were thawed in a 37

(83) Tryptophan Indole Metabolites Reduce Anastomotic Leakage Through Aryl Hydrocarbon Receptor-driven Interleukin-22 ProductionCellular and Molecular Gastroenterology and HepatologyFebruary 16, 2026Vasiliki Bantavi, Laura Gloeck, Patrick Leven, Konstantina Zafeiropoulou, Olaf Welting, Hilal Sengül, Patrik Efferz, Bianca Schneiker, Timothy Ryan McCulloch, Christoph Wilhelm, et al.washing, intracellular antigens were stained for 1 hour at room temperature. Data was acquired on a Sony ID7000 (Sony Biotechnology) and analysis performed using FlowJo software (v10.9, Treestar). Fluorescent in Situ Hybridization

(84) Identification of multiple Acinetobacter baumannii protein antigens as targets for potential immunotherapies using a novel protein microarray screening approachPLOS PathogensFebruary 12, 2026Samantha Palethorpe, Giuseppe Ercoli, Elisa Ramos-Sevillano, Gathoni Kamuyu, Joe Campo, Samuel Willcocks, Rie Nakajima, Philip Felgner, Brendan Wren, Ganjana Lertmemongkolchai, et al.baumannii) were performed as previously described [33,35] and analysed using a BD FACSVerse or Sony ID7000 Spectral Analyser and FlowJo software for Windows (Version 10). Animal studies Mice were obtained

(85) Lineage-matched Oropouche virus mRNA-LNP vaccines confer complete, cross-protective immunity in micemBioFebruary 11, 2026Yumiko Yamada, Inho Cha, Soowon Kang, Wan-Shan Yang, Morgan Lewis, Chloe Chung, Woo-Jin Shin, Jun Bae Park, Nam-Hyuk Cho, Young-Ki Choi, et al.fixed with 4% paraformaldehyde (PFA) at room temperature for 15 min. Prior to acquisition on the SONY ID7000 Spectral Cell Analyzer (Sony Biotechnology), cells were kept in the dark at 4°C in FACS buffer containing Tandem Stabilizer

(86) Trackable Tolerogenic Macrophages Integrate PD‐L1 and Rapamycin Signaling to Suppress Alloimmune Responses in TransplantationAdvanced ScienceFebruary 8, 2026Yihui Wang, Yuan Song, Yuji Xie, Junmin Zhang, Jing Liu, Wenyuan Wang, Ying Xu, Wenqian Wu, Wuqi Zhou, Qiaofeng Jin, et al.transferred to flow cytometry tubes equipped with a filter. Finally, analysis was conducted using a Sony ID7000 spectral flow cytometer, configured with DiI parameters set to an excitation wavelength of 549 nm and

(87) Interleukin-10 expressing B lineage cells in visceral adipose tissue protect against aging-related insulin resistance and extend lifespanNature CommunicationsFebruary 7, 2026Jielong Guo, Xue Han, Yue Qin, Kexin Hong, Yuchen Lin, Shuping Han, Ning Hou, Lihui Cao, Xiaoxiang Gao, Weidong Huang, et al.resuspended with a cell staining buffer containing precision count beads. Data were acquired with Sony ID7000 Spectral Cell Analyzer and analyzed with Flowjo V10. Bregs isolation Live Bregs (IgM+IL10+) were sorted from the single cell

(88) Succinate receptor 1 restricts hematopoiesis and prevents acute myeloid leukemia progressionNature CommunicationsFebruary 5, 2026Vincent Cuminetti, Emeline Boet, Marcel Heugel, Joanna Konieczny, Aurora Bernal, Manuel J. Gomez, Franco Grimolizzi, Nuria Vilaplana-Lopera, Marc Ferré, Alicia Villatoro, et al.Dickinson (BD) Canto or LSR Fortessa analyzer with DIVA software v6.1.3, v8.0.1 and v9.0, or a SONY ID7000 spectral flow cytometer with ID7000 software v2.02.17121. For transplant of HSPC from Sucnr1-tdTomato

(89) Altered B cell activation contributes to the immunopathogenesis of childhood arthritis-associated uveitisNat CommunFebruary 3, 2026Bethany R Jebson, Benjamin Ingledow, Vicky Alexiou, Jakub Kubiak, Persephone Jenkins, Yuxuan Meng, Melissa Kartawinata, Restuadi Restuadi, Wei-Yu Lin, Chris Wallace, et al.FACS buffer for analysis on an LSR II cell analyser (BD Biosciences) or Sony spectral cell analyser ID7000 (Sony Biotechnology). For the details of antibodies used for human standard panels, see Supplementary

(90) Transcriptomic landscape of transposable elements reveals LTR7 - PLAAT4 as a potential oncogene and therapeutic target in pancreatic adenocarcinomaGenome ResearchFebruary 3, 2026Meilong Shi, Chuanqi Teng, Shan Zhang, Xiaobo He, Lingyun Xu, Fengxian Han, Rongqi Wen, Ganjun Yu, Jingwen Liu, Yang Feng, et al.PE-labeled anti-HLA-A2.1 (BD Biosciences, #551230, RRID:AB_394106) and analyzed by flow cytometry (Sony ID7000). Fluorescence intensity (FI) was calculated; FI > 1 indicated strong binding. ELISPOT assay PBMCs

(91) Maternal-fetal immune conflict contributes to male-specific impairments in a mouse model of neurodevelopmental disordersbioRxivFebruary 3, 2026Irene Sanchez-Martin, Bharti Kukreja, Paige Henderson, Qianyu Lin, Daniel DiMartino, Valerie Bagan, Justin Park, Brian T. Kalish, Lucas Cheadlecapture beads, together with an unstained control, and acquired in parallel. Data were acquired on a Sony ID7000 spectral flow cytometer, and spectral unmixing, including autofluorescence extraction when applicable,

(92) Migraine-induced cochlear injury triggers ZBP1-mediated PANoptosis via CGRP signalingThe Journal of Headache and PainFebruary 2, 2026Wandi Xu, Ni Zhai, Jingyu Chen, Shun Zhou, E. Tian, Zhaoqi Guo, Zhanghong Zhou, Xixi Yu, Ziyu Zhai, Xin Zhang, et al.Samples were analyzed within 1 h of staining. The stained cells were analyzed by flow cytometry (Sony ID7000, Japan), and the apoptosis rate was analyzed using FlowJo software (Version 10.8.1). Western blot The

(93) Redosing of anti-CD3 antibodies in NOD mice with new-onset diabetes does not alter the effect of a single treatment courseDiabetologiaFebruary 1, 2026Amber Wouters, Pierre Lemaitre, Laure Degroote, Marijke Viaene, Marc Packbier, Nick Geukens, Chantal Mathieu, Conny GysemansAntibodies used in flow cytometry studies can be found in ESM Table 1. Samples were acquired on the Sony ID7000 Spectral Cell Analyzer (Sony Biotechnology, San Jose, CA, USA) and data were analysed via FlowJo software (v10.8.1; Becton

(94) Islet regeneration protein Reg3g promotes macrophage clearance of β cell-derived dysfunctional mitochondria-rich vesicles to mitigate T2DMRedox BiologyFebruary 1, 2026Senlin Li, Mengheng Wang, Hong Zhou, Jia Liu, Mengxuan Wang, Huimin Yuan, Mengzhu Zheng, Xin Li, Xiangling Zhu, Ming XiangAPC-CD206, APC-CD11b, which were manufactured by BD Biosciences. Flow cytometry was performed using a Sony ID7000 (Sony, Tokyo, Japan) or BD C6 flow cytometer (BD Accuri), and the data were processed via FlowJo 10

(95) BioMarrow: An accessible and reproducible 3D patient-derived bone marrow model for advancing research and clinical applicationsTranslational OncologyFebruary 1, 2026Diana Lourenço, Raquel Lopes, Joana Caetano, Filipa Barahona, Jessica Rodrigues, Ana C. Queirós, Emilie Arnault Carneiro, Cristina Joãoas negative control. Each sample was run in duplicate. The fluorescent signal was acquired using an ID7000™ Spectral Cell Analyzer (Sony, Tokyo, JPN) and quantified using the LegendPlex™ Qognit software. Statistical analysis Statistical

(96) CHB‐Induced Immune Zonation Chaos Elicited LXRα‐mediated Lipid Metabolism Disorders in Kupffer Cells to Induce Cancer Stem Cell FormationAdvanced ScienceFebruary 1, 2026Jingqi Shi, Qingyu Li, Jian Li, Jian Bai, Ji Xi, Qi He, Jianglin Zhou, Xuejun Wang, Xiang Song, Xiaoju Li, et al.Biolegend were used to detect cell stemness of HepAD38 cell line. Flow cytometry was performed using Sony ID7000 Spectral Cell Analyzer (Sony Biotechnology Inc., USA) and analyzed using FlowJo 10.5.0 software. Southern Blotting of viral

(97) Safety and biologic activity of a bispecific T cell receptor targeting HIV Gag in males living with HIV: a first-in-human trialNature CommunicationsJanuary 31, 2026Linos Vandekerckhove, Julie Fox, Borja Mora-Peris, Jordi Navarro, Sabine D. Allard, Alison J. Uriel, Santiago Moreno Guillén, Marta Boffito, Frank A. Post, Vicente Estrada, et al.and intracellular staining for 20 min at RT. Cells were fixed in 4% PFA, washed, and acquired on the ID7000 spectral analyser (Sony). Antibodies used in this panel are described in Supplementary Table 2. Flow cytometry analysis

(98) Development and computational analysis of high dimensional spectral flow cytometry data for the resolution of innate lymphoid cells in the mammary tumor microenvironmentFrontiers in ImmunologyJanuary 27, 2026Hobin Seo, Jingna Xue, Qiutong Huang, Megan Kinzel, Amisha Verma, Ngan Huynh, Zahra Jamila Ikra, Douglas J. Mahoney, Jongbok Lee, Sorana Morrissy, et al.flow cytometry buffer and acquired on a 5-laser Cytek Aurora System (Cytek Biosciences) or 6-laser Sony ID7000 Spectral Cell Analyzer (Sony Biotechnology). For direct comparison between the two instruments, we divided samples into two

(99) Mast cell-specific CysLT2 receptor signaling inhibits cysteinyl leukotriene-dependent mast cell activation and type 2 allergic lung inflammationCell ReportsJanuary 27, 2026Jun Nagai, Madeline M. Hastings, Airi Nishida, Sreyashi Majumdar, Kendall Zaleski, Sofia A. Marshall, Junrui Lin, Mira A.S. Saliman, Yumi Tani, Yamato Murakami, et al.Biosciences,https://www.flowjo.com Prism 9 statistical analysis,GraphPad,https://www.graphpad.com/features ID7000 Spectral Cell Analyzer,SONY,https://www.sonybiotechnology.com/instruments/id7000-spectral-cell-analyzer TC20™ Data Analyzer

(100) Doubling dolutegravir dosage reduces the viral reservoir in ART-treated people with HIVeLifeJanuary 23, 2026Céline Fombellida-Lopez, Aurelija Valaitienė, Lee Winchester, Nathalie Maes, Patricia Dellot, Céline Vanwinge, Aurélie Ladang, Etienne Cavalier, Fabrice Susin, Dolores Vaira, et al.from BD Biosciences). Cells were then washed, fixed in 1% paraformaldehyde, and run on a FACS SONY ID7000. Analysis was performed using FlowJo software (v10.8.1). Cells were gated according to morphological

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ID7000™ Spectral Cell Analyzer

ID7000™ Spectral Cell Analyzer from Sony Corporation

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ID7000™ Spectral Cell Analyzer from Sony Corporation

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