ID7000™ Spectral Cell Analyzer from Sony Corporation

Product Specs
ItemID7000™ Spectral Cell Analyzer
CompanySony Corporation
Price Inquire
Catalog NumberID7000
Detection32-channels (PMT arrays); can be configured with 186 detectors to detect 44 colors or more
Throughput40,000 events per second
Lasers3 to 7 lasers
Excitation320 nm, 355 nm, 405 nm, 488 nm, 561 nm, 637 nm, 808 nm
Parameters186 fluorescence channels
CapacityTubes (5 mL), Microplates using autosampler (96-well, 384-well)
Data Throughput40,000 events/sec
Dimensions(WxDxH) 106 x 71.9 x 76 cm
Flow Rate33 to 200 µL/min

(21) PEBP4 alleviates muscle wasting in lung cancer cachexia via KEAP1–NRF2-mediated redox homeostasisCell Death & DiseaseJune 3, 2026Yaru Xia, Yuqi Han, Weiquan Li, Tiexi Yu, Diaoyi Tan, Daojia Miao, Wen Li, Jiaming Wu, Qianqian Luo, Jiemin Li, et al.serum-free medium containing DHE for 1 h at 37 °C, then collected, washed, and analyzed by flow cytometry (ID7000, Sony, Japan; 10,000 events/sample). ROS fluorescence intensity was quantified using FlowJo software

(22) Mechanostimulatory Cues Determine Intestinal Fibroblast Fate and Profibrotic Remodeling in a Physiodynamic Human Gut‐on‐a‐ChipAdvanced ScienceJune 1, 2026Soyoun Min, Nam Than, Yong Cheol Shin, Elif G. Ertugral, Chandrasekhar R. Kothapalli, Olumuyiwa Awoniyi, Hyun Jung Kimcat. no. IC1918P). After staining, cells were washed, resuspended in buffer, and analyzed on a Sony ID7000 Spectral Cell Analyzer (Sony Biotechnology). Data were processed using FlowJo software (Version 10; BD Biosciences). Fluorescence‐minus‐one

(23) Attenuated FTO induces necroptosis of alveolar epithelium via the m6A/CYP1B1 /ROS/MLKL axis to promote the aggravation of pulmonary emphysemaRedox BiologyMay 27, 2026Mindan Wu, Wenjing Wang, Zemin Chen, Yuanlan Yang, Jiawei Yan, Yachao Cui, Peiyu Huang, Feng Zhang, Wei Tu, Wensheng Chen, et al.Finally, immunophenotyping and population quantification were conducted using flow cytometry (Sony ID7000). 5.5 Histology and MLI After euthanasia, mouse lungs were inflation-fixed via tracheal cannulation

(24) Pneumococcal membrane particles promote serotype-independent cellular and humoral immunity and protect against pneumococcal colonizationProc Natl Acad Sci U S AMay 26, 2026Rebecca Dookie, Ana Rita Narciso, Meztlli O. Gaytán, Anandi Narayana Moorthy, Sayoni Chakraborty, Sigrun Thorsdottir, John Boss, Francesco Righetti, Jens Karlsson, Max Heurgren, et al.then stained for flow cytometry as described above. Samples were acquired on either BD LSR II or Sony ID7000 Spectral Cell Analyzer and analyzed with FlowJo Software. Unsupervised clustering analysis was carried out on live, single,

(25) Tumor implantation site dictates the immune landscape of syngeneic mouse models of head and neck cancerScientific ReportsMay 20, 2026Klara Rasmussen Bollerup Lanng, Anders Etzerodt, Michael Robert Horsman, Brita Singers Sørensen, Martin Roelsgaard Jakobsensubsequently treated as either the TAM panel or the TIL panel. The samples were analyzed on a 5-laser SONY ID7000 spectral flow cytometer (SONY Biotechnologies, USA), available at the FACS core facility at the Department

(26) Q1020R in the spike proteins of MERS-CoV from Arabian camels confers resistance against soluble human DPP4Journal of VirologyMay 19, 2026Nianzhen Chen, Hannah Kleine-Weber, Khaled Alkharsah, Michael Winkler, Asisa Volz, Marcel A. Müller, Victor M. Corman, Christian Drosten, Markus Hoffmann, Stefan Pöhlmannthe cells were washed with PBS-B, resuspended in PBS-B, and then analyzed via flow cytometry using an ID7000 Spectral Cell Analyzer (Sony Biotechnology, San Jose, CA, USA). Camel and human plasma samples No sampling of camels was

(27) Nano-granulated zoledronate sensitizes innate immune metabolism to enhance vaccine-induced and antitumor immunityCell Reports MedicineMay 19, 2026Meifang Chen, Xiaojia Jiao, Zhicheng Yan, Yueheng Wang, Jing Zhang, Qinghua Wu, Minghui Li, Shumin Fan, Yuan Wang, Wenbing Dai, et al.different antibodies. Finally, the stained cells were filtered through a strainer, acquired on the ID7000 spectral flow cytometer(Sony, Japan), and analyzed using Flowjo. Tetramer staining H2-Kb/OVA tetramer

(28) GLP1-E2 therapy delays autoimmune diabetes in late-stage prediabetic NOD mice and potentiates low-dose anti-CD3 therapy for enhanced disease protectionDiabetologiaMay 18, 2026Laure Degroote, Jędrzej Chrzanowski, Pierre Lemaitre, Amber Wouters, Stephanie Bourgeois, Annelore Van Mulders, Julie Pierreux, Sophie Coenen, Lien Willems, Gunter Leuckx, et al.FoxP3 APC (eBioscience, clone FjK-16S, 17-5773-82, 1:300). Sample acquisition was performed on a Sony ID7000 Spectral Cell Analyzer (Sony Biotechnology, San Jose, CA). Data were analysed with FlowJo (v.10.8.1, FlowJo). Insulitis grading FFPE

(29) Natural senolytic activity of Rhodiola rosea extract alleviates age-associated phenotypes via paraptosisiScienceMay 15, 2026Ryo Furuuchi, Yohko Yoshida, Goro Katsuumi, Takaaki Furihata, Yusuke Joki, Chieh-Lun Hsiao, Masayoshi Suda, Hana Saito, Tamano Kumazaki, Hidefumi Makabe, et al.following the manufacturers’ protocols. Stained cells were analyzed using the Spectral Cell Analyzer ID7000 (Sony), and the proportions of PI-positive cells were quantified. Systemic metabolic parameter For

(30) Feeder-free generation of functional dendritic cells from human pluripotent stem cellsJ Immunother CancerMay 13, 2026Wei-Hung Jung, Andrew Khalil, Yoav Binenbaum, Joshua M Price, Kyle H Vining, Tenzin Lungjangwa, Miguel C Sobral, Rudolf Jaenisch, David J Mooneybuffer, and analyzed on a CytoFLEX LX (Beckman Coulter), Cytek Aurora (Cytek Biosciences), or Sony ID7000 (Sony Biotechnology) flow cytometer. For MART-1 and BMLF-1 tetramer assembly, phycoerythrin (PE)-conjugated

(31) Engineered virus-hunter vaccine overcomes HBV immune toleranceGutMay 12, 2026Xiaoqing Chen, Xue Liu, Tao Xu, Yalin Wang, Peng Wang, Han Liu, Yichao Jiang, Xiaojing Qin, Liang Zhang, Yueting Xiong, et al.(BioLegend, 652406) and anti-mouse FoxP3-PE-CF594 (BD, 567373). Spectral flow data were acquired on an ID7000 Spectral Cell Analyzer (Sony) and analysed via FlowJo software. ELISpot assay For the HBsAg-specific B-cell ELISpot assay,

(32) A RIG-I targeting nanozyme induces PANoptosis for cancer immunotherapyNature CommunicationsMay 11, 2026Shuohui Dong, Ye Yuan, Haolin Cao, Mingguang Zhang, Shuo Liang, Mingxiao Zhang, Xiangsheng Su, Li Luo, Xiang Zhao, Linchuan Li, et al.(Biolegend) for 30 min on ice, protected from light. The stained cells were then analyzed using an ID7000™ spectral cell analyzer flow cytometer (Sony) and the data were processed with FlowJo software (version 10). RNA extraction

(33) ERα blockade in dendritic cells enhances antigen cross-presentation and induces antitumor CD8+ T cell immunityNature CommunicationsMay 5, 2026Yaxin Liu, Zhongfei Ma, Xuelian Li, Yuanyuan Ding, Zhiqing Li, Bin Zhang, Yumei Zhou, Jun Zhou, Shuo Liu, Shuxun Liu, et al.BV510-anti-human CD40 antibody and PE-Cy7-anti-human CD274 antibody for 30 min. Stained cells were analyzed by ID7000 (SONY) BD LSRFortessa or BD FACSymphony A1 flow cytometer and analyzed using FlowJo version 10 software.

(34) Matrix Viscoelasticity Regulates Dendritic Cell Migration and Immune PrimingAdvanced MaterialsMay 5, 2026Wei‐Hung Jung, Emie Humann, Joshua M. Price, Yoav Binenbaum, Azra Haseki, Sanjana Iyer, David J. Mooneythe cells were washed twice with FACS buffer, resuspended in FACS buffer, and analyzed using a Sony ID7000. Gating was performed using fluorescence-minus-one controls. The gating strategy for CD4+ and CD8+ T

(35) Allogeneic CRISPR-Engineered CAR-T Cells Drive Potent Antitumor Activity in Solid TumorsbioRxivMay 5, 2026Mingyu Huo, Dan Li, Nan Li, Alex Quan, Tianyuzhou Liang, Dan Henderson, Jason Sagert, Minh Pham, Luke Hanley, Kelly Maeng, et al.cryopreserved. Flow cytometry All flow cytometry experiments were conducted and analyzed using the SONY ID7000 (Sony Biotechnology) and the FlowJo software (BD Biosciences). The anti-EGFR human monoclonal antibody

(36) Nasal CD4+ tissue-resident memory T cells provide cross-protective immunity to influenzaJ Exp MedMay 4, 2026Nimitha R. Mathew, Romain Gailleton, Lydia Scharf, Karin Schön, Josue Enriquez, Hannes Axelsson, Anneli Strömberg, Nils Lycke, Mats Bemark, Ka-Wei Tang, et al.cells were washed and suspended in FACS buffer for data acquisition at BD LSRFortessa X-20 or Sony ID7000 spectral analyzer. Flow cytometry of murine cells All fluorochrome-conjugated antibodies are titrated

(37) CD163 and Tim-4 identify resident intestinal macrophages that are spatially regulated by TGF-βJ Exp MedMay 4, 2026Vignesh Jayaraman, Ian E. Prise, Verena Kästele, Sabrina Tamburrano, Kelly Wemyss, Hayley M. Bridgeman, Rufus H. Daw, Patrick Strangward, Alfie Sanderson, Sheena M. Cruickshank, et al.Scientific. Cell acquisition was performed on an LSRFortessa running FACSDIVA software (BD) or SONY ID7000 (SONY). For each intestinal sample, typically 10,000–20,000 macrophages were collected. Data were analyzed

(38) YY1 Lactylation Elicits CARD9 Deficiency in Dendritic Cells Promoting Pancreatic Cancer Immune EscapeInt J Biol SciMay 1, 2026Lingyan Ding, Cheng Tian, Yiting Feng, Ruochen Xu, Senlin Li, Mengzhu Zheng, Xiaoyan Wang, Qianqian Xu, Ming Xiangstatus of T cells was detected on the machine. Flow cytometry Flow cytometry was performed using Sony ID7000 (Sony, Tokyo, Japan) or C6 flow cytometer (BD Accuri), and the data were processed via FlowJo 10. Samples

(39) Single-cell lineage tracing identifies hemogenic endothelial cells in the adult mouse bone marrowElifeApril 27, 2026Jing-Xin Feng, Mei-Ting Yang, Lili Li, Caiyi C Li, Ferenc Livak, Jack Chen, Yongmei Zhao, Dunrui Wang, Avinash Bhandoola, Naomi Taylor, et al.acquired with BD FACSCanto-II, BD LSRFortessa, BD FACSymphony A5 (BD Biosciences), Sony SA3800 or Sony ID7000 cell analyzers. Cell sorting was performed with BD FACS Aria III, BD FACS Aria Fusion, or Sony SH800S

(40) Single-cell lineage tracing identifies hemogenic endothelial cells in the adult mouse bone marroweLifeApril 27, 2026Jing-Xin Feng, Mei-Ting Yang, Lili Li, Caiyi C Li, Ferenc Livák, Jack Chen, Yongmei Zhao, Dunrui Wang, Avinash Bhandoola, Naomi Taylor, et al.acquired with BD FACSCanto-II, BD LSRFortessa, BD FACSymphony A5 (BD Biosciences), Sony SA3800 or Sony ID7000 cell analyzers. Cell sorting was performed with BD FACS Aria III, BD FACS Aria Fusion, or Sony SH800S

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ID7000™ Spectral Cell Analyzer

ID7000™ Spectral Cell Analyzer from Sony Corporation

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ID7000™ Spectral Cell Analyzer from Sony Corporation

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