ID7000™ Spectral Cell Analyzer from Sony Corporation

Product Specs
ItemID7000™ Spectral Cell Analyzer
CompanySony Corporation
Price Inquire
Catalog NumberID7000
Detection32-channels (PMT arrays); can be configured with 186 detectors to detect 44 colors or more
Throughput40,000 events per second
Lasers3 to 7 lasers
Excitation320 nm, 355 nm, 405 nm, 488 nm, 561 nm, 637 nm, 808 nm
Parameters186 fluorescence channels
CapacityTubes (5 mL), Microplates using autosampler (96-well, 384-well)
Data Throughput40,000 events/sec
Dimensions(WxDxH) 106 x 71.9 x 76 cm
Flow Rate33 to 200 µL/min

(41) Palmitic Acid Promotes Antiviral Innate Immunity via ZDHHC20‐Mediated CMPK2 PalmitoylationAdvanced ScienceApril 21, 2026Yujia Wang, Zenghui Cui, Yunkai Zhang, Zhiqing Li, Xuetao Caospleens were stained with fluorescent‐conjugated primary antibodies. Cells were detected using a SONY ID7000, and the data were analyzed using FlowJo software (Tree Star). 4.10 Immunoblot Assay and Co‐Immunoprecipitation

(42) Breast tumor microbiome regulates anti-tumor immunity and T cell-associated metabolitesScientific ReportsApril 16, 2026Chin-Chih Liu, Dennis Grencewicz, Karthik Chakravarthy, Lin Li, Ruth Liepold, Matthew Wolf, Lynn M. Marcho, Naseer Sangwan, Alice Tzeng, Rebecca Hoyd, et al.room temperature with antibodies targeting intracellular markers. Samples were analyzed using a SONY ID7000™ spectral cell analyzer. The resulting FCS data files were then analyzed by FlowJo™ Software v10.8.0 (BD Life Sciences) with

(43) Hypoxia responses in arginase 2 deficient mice enhance cardiovascular healthbioRxivApril 11, 2026Weiling Xu, Kewal Asosingh, Allison J. Janocha, Evan Madden, Nicholas Wanner, Dylan Trotter, Michael V. Novotny, Anny Mulya, Samar Farha, Serpil C. Erzurumresuspended in 500 μL of 1% BSA with 7-AAD (BD 559925; 1.25 μg/500μL). Data were acquired using the Sony ID7000 spectral flow cytometer equipped with 6 lasers (320nm, 355nm, 405nm, 488nm, 561nm, 637nm) collecting

(44) Impaired removal of dying brain cells by microglia in Gpr34 deficient miceJournal of NeuroinflammationApril 10, 2026Diana G. Bohannon, Ana Geller, Sean K. Simmons, John A. Cintron, William E. Martenis, Katherine Stalnacker, Autumn Masse, Min Jee Kwon, Yan-Ling Zhang, Joshua Z. Levin, et al.basic protein (MBP), and Snap25 (Supplemental Table 1b). Samples were then washed and imaged on a Sony ID7000 along with appropriate single color, unstained, and fluorescent minus one (FMO) controls. Unmixing was

(45) Associations between food consumption with T cell activation and antibody responses following SARS-CoV-2 mRNA vaccinationGut Microbes ReportsMarch 30, 2026Hiroki Negishi, Gaku Nakato, Rie Kadowaki, Hiroki Kono, Mami Minakata, Ayumi Ichikawa, Takayuki Toshimitsu, Seiya Makino, Hiroshi Kano, Sho Nakamura, et al.780 (Invitrogen, USA). Stained cells were fixed with Cytofix/Cytoperm™ (BD) and acquired using the ID7000™ Spectral Cell Analyzer (Sony, Japan). Data were analyzed using FlowJo software. Flow cytometry gating strategy is detailed

(46) Tracking spatio-temporal dynamics of early immune responses to an intranasal OMV-based pneumococcal vaccine candidate in micenpj VaccinesMarch 30, 2026Sajida Kanwal, Shaina Vivienne To, Rienke Uijen, Rona Roverts, Bram van Cranenbroek, Fred J. van Opzeeland, Ben Joosten, Bart van den Berg van Saparoea, Christa E. van der Gaast-de Jongh, Esther van Rijssen, et al.After washing the cells twice, the samples were resuspended in the buffer and data was acquired on an ID7000 5 L spectral flow cytometer (Sony). 100,000 events were included per sample. Identical protocol was

(47) Circulating exhausted CD8+ effector memory cells differentiate immune checkpoint inhibitor-induced liver injury from other acute immune-mediated liver injuriesJ Immunother CancerMarch 27, 2026Stuart Astbury, Edmond Atallah, Jane I Grove, Amber G Bozward, Scott P Davies, Mark J Sheehan, Steven W Kumpf, Jessie Qian, Natalia M Krajewska, Grace E Wootton, et al.antibodies and matched isotype controls (online supplemental table 3) and data were acquired on an ID7000 spectral flow cytometer (Sony Biotechnology). Data were analyzed in FlowJo V.10 software. Fluorescence

(48) CBX2 phase-separation contributes to homologous recombination repair and drug resistance in ovarian cancerCell Death & DiseaseMarch 26, 2026Si Sun, Lin Huang, Yujia Ma, Zheng Wei, Mengna Zhu, Mengqing Chen, Feiquan Ying, Xiaoling Zhou, Ping Yang, Yiping Wen, et al.or 556547) according to the manufacturer’s instructions. Flow cytometry analysis was conducted on an ID7000 Spectral Cell Analyzer (Sony Biotechnology). Western blot Cells were harvested and lysed using the Mammalian Cell & Tissue

(49) Genetically engineered eye-colonizing microbes that deliver the anti-inflammatory cytokine interleukin-10 enhance corneal tissue repairCell ReportsMarch 24, 2026Jackie Shane, Matthew Evans, Yannis Rigas, Robert M.Q. Shanks, Anthony J. St. Legerpanels used are detailed in Table S2. Samples were analyzed on a Beckman Coulter Cytoflex LX or a Sony ID7000 spectral cytometer. mIL-10 effect on in-vivo corneal wounds To evaluate the effect of the mIL-10 producing

(50) Application of spectral flow cytometry for comprehensive detection of immune metabolism in patient-derived microsamplesCell Rep MethodsMarch 23, 2026Yang Bai, Yuqing Wang, Yicheng Fu, Zhengyang Guo, Zhaoyuan Liang, Liu Yang, Jiawei Ribaudo, Dan Liu, Yanfang Li, Ting Zhang, et al.probes for 30 min at 37°C. Cells were washed and then acquired by full spectrum flow cytometry (SONY ID7000). The working concentration of all fluorescent probes are listed in Table 1. Negative and positive controls

(51) Systematic profiling of SARS-CoV-2 structural protein-specific T cell epitopes in Omicron infections following inactivated vaccinationiScienceMarch 20, 2026Zhiqing Li, Mengmeng Cui, Jian Wu, Tianju Hu, Junyan Dan, Xiaosu Chen, Qicong Shen, Jin Hou, Zhongfang Wang, Yizhi Yu, et al.Cells were washed with Perm/Wash™ Buffer and acquired within 24 hours on BD LSRFortessa™ X-20 or Sony ID7000™ spectral cytometers. Flow cytometry data were analyzed using FlowJo™ v10.10.0 . Systematic high-throughput screening

(52) Flow cytometric evaluation of monoclonal antibodies for cross-reactivity with feline leukocytesFrontiers in Veterinary ScienceMarch 18, 2026H. Krüger, A. Moritz, B. Roshani, S. Neumanndiscarded, and the cells were resuspended in 150 μL PBS/5% BSA for analysis. Cells were acquired using the ID7000™ spectral analyzer (Sony Biotechnology, San Jose, CA, USA). Unmixing was calculated using single-stained

(53) The MEK1/2-IRF4 axis fosters T follicular helper cell differentiationand antitumor humoral immune responseJournal of Translational MedicineMarch 16, 2026Shuan Ran, Song Wang, Ran Li, Longyong Lai, Jizhang Yu, Xi Zhang, Yuan Li, Weicong Ye, Junjie Zong, Xiaohan Li, et al.(Biolegend, #406421) at 37 °C for 30 min. After staining as described above, the cells were processed with an ID7000 flow cytometer (Sony) or an LSR Fortessa X-20 flow cytometer (BD Biosciences), and the data were analyzed

(54) BZW1 Drives Immune Evasion in Lung Adenocarcinoma via Ferroptosis SuppressionAdvanced ScienceMarch 15, 2026Linyao Zhao, Yue Peng, Qing Liang, Shi Liu, Yang Li, Lei Ma, Menghan Hu, Sujuan Zheng, Zhihua Liu, Shugeng Gaocold PBS and resuspended in 200 µL PBS for immediate analysis. Flow cytometry was performed using an ID7000 Spectral Cell Analyzer (Sony Biotechnology, Japan), with the non‐oxidized form detected in the PE channel (excitation/emission:

(55) Establishment of a humanized patient‐derived xenograft mouse model of high‐grade serous ovarian cancer for preclinical evaluation of combination immunotherapyMolecular OncologyMarch 14, 2026Luka Tandaric, Line Bjørge, Martine Rott Lode, Cecilie Fredvik Torkildsen, Pia Aehnlich, Rammah Elnour, Daniela Elena Costea, Lars Andreas Akslen, Liv Cecilie Vestrheim Thomsen, Emmet McCormack, et al.defined in Table S1. Stained cells were washed twice with a mix of 2% V/V FBS in PBS and acquired on an ID7000 Spectral Cell Analyzer (LE‐ID7000C; Sony Biotechnology) equipped with ID7000 Software (v2.0.2.17121; Sony Biotechnology). Single‐cell

(56) Ageing promotes metastasis via activation of the integrated stress responseNatureMarch 11, 2026Angana A. H. Patel, Jozefina J. Dzanan, Kevin X. Ali, Ella A. Eklund, Samantha W. Alvarez, Dorota Raj, Martin Dankis, Ilayda Altinönder, Maria Schwarz, Kristell Le Gal, et al.suspensions were prepared from spheroids on days 0, 2, 4, 6 and 8, flow cytometry data were collected on Sony ID7000 Spectral Cell Analyzer and analysed using Flow Jo software v.10.8.1. Lentiviral production and transduction Lentiviruses

(57) Multifunctional copper-based nanoparticles potentiate colorectal cancer immunotherapy via synergistic metabolic remodeling and cGAS-STING pathway activationJournal of NanobiotechnologyMarch 8, 2026Maopu Tu, Xiaoyu Deng, Zhiyong Zhou, Xiaodong Li, Shengxun Mao, Bin Lai, Lisong Pang, Qilin Zhong, Jiaqing Cao, Haipeng Liu, et al.(CLSM, Leica Stellaris STED). Parallel samples were analyzed by flow cytometry (SONY Group Corporation ID7000). For intracellular copper analysis, cells were collected after 6 h incubation with formulations, washed

(58) NCOA2 promotes the return of hematopoietic stem cells to quiescence after irradiation stress by regulating FOXO3a-dependent mitophagyHemasphereMarch 7, 2026Fang Chen, Naicheng Chen, Linjie Wang, Hao Zeng, Yukai Lu, Yanying Liu, Jing Chen, Yang Xu, Mo Chen, Song Wang, et al.Alexa Fluor 488 (#A‐11034, Invitrogen, Carlsbad, CA, USA). Flow cytometric analysis was performed by ID7000 (Sony Biotechnology, Japan), FACSverse (BD Biosciences), or FACSymphonyTM A1 (BD Biosciences), and the

(59) aGPCR-HEK: A Stable High-Expression Inducible Mammalian Cell Expression System for Adhesion GPCR Structural Biology ApplicationsBio ProtocMarch 5, 2026David M. Favara, Christopher G. TateInvitrogen Bigfoot Cell Sorter (Invitrogen, catalog number: PL00304) 9. Flow cytometer (Sony, model: ID7000 Spectral Cell Analyzer) 10. FSEC system: Shimadzu HPLC system comprising the following components: Shimadzu Degasser Module

(60) Purification of the Active-State G Protein-Coupled Receptor ADGRL4 for Cryo-Electron Microscopy Using a Modular Tag System and a Tethered mini-G_qBIO-PROTOCOLMarch 5, 2026David M. Favara, Christopher G. Tate300 mesh Au grids (Quantifoil, catalog number: Q350AR13A) Equipment 1. Flow cytometer (Sony, model: ID7000 Spectral Cell Analyzer) 2. Temperature-controlled floor-standing centrifuge for 1 L bottles (Beckman Coulter, Avanti JXN-26

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ID7000™ Spectral Cell Analyzer

ID7000™ Spectral Cell Analyzer from Sony Corporation

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ID7000™ Spectral Cell Analyzer from Sony Corporation

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