Fixation Buffer from BioLegend

Product Specs
ItemFixation Buffer
CompanyBioLegend
Price $59.00Supplier Page View Company Product Page
Catalog Number420801
ApplicationsICFC,ICC
Quantity100ML

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(161) Bezafibrate-driven mitochondrial targeting enhances antitumor immunity and prevents lung cancer via CD8+ T cell infiltration and MDSC reductionFront ImmunolApril 15, 2025Jing Pan, Jiaheng Li, Qi Zhang, Mofei Huang, Yian Wang, Ming Youstaining, cells were first stained with surface markers, then fixed with fixation buffer (Biolegend, 420801), permeabilized with FoxP3/Transcription Factor Staining Buffer Set (eBioscience, 00-5523-00), and stained

(162) FTO controls CD8+ T cell survival and effector response by modulating m6A methylation of FasCell Death DisApril 15, 2025Lina Sun, Tianzhe Zhang, Yao Ge, Zhihong Yao, Yanhong Su, Qianhao Wang, Yang Chen, Boxiao He, Renyi Ding, Cangang Zhang, et al.The stimulated cells were fixed and permeabilized using a Fixation/Permeabilization Solution Kit (#420801 and #421002, Biolegend). For nuclear protein staining, such as Ki67, T-bet, Eomes, and Bcl-2, cells

(163) Inhibition of the CXCR4/PLC Signaling Increases Dexamethasone-Induced Sensitivity by Activating the Mitochondrial Apoptotic Pathway in B-Cell Acute Lymphoblastic LeukemiaInt J Mol SciApril 8, 2025Souleymane Abdoul-Azize, Jean-Pierre Vannier, Pascale Schneiderassessed by intracellular staining flow cytometry [35]. After fixation with buffer (Biolegend, cat. no. 420801) and permeabilized with True-Phos Perm Buffer (Biolegend, cat. no. 425401), cells were stained PE anti-BIM

(164) Double-negative T cells in combination with ursodeoxycholic acid ameliorates immune-mediated cholangitis in miceBMC MedApril 7, 2025Chunpan Zhang, Guangyong Sun, Hua Jin, Yunxiong Wei, Shimeng Zheng, Xiyu Wang, Xinyan Zhao, Dong Zhang, Jidong Jia°C. For intracellular staining, the cells were first fixed with Intracellular Fixation Buffer (Cat#: 420801, Biolegend, USA) for 40 min at 4 °C. Next, the cells were permeabilized with Permeabilization Buffer

(165) The crosstalk of monocyte-neutrophil in hair follicles regulates neutrophil transepidermal migration in contact dermatitisCommun BiolApril 4, 2025Zhan Fan, Yilun Xu, Yafang Lu, Xinlin Li, Mengli Xu, Jinxin Liu, Zhenzhen Cai, Jiayang Liu, Jingping Su, Jialu Wang, et al.markers, followed by fixation and permeabilization at 4 °C for 20 min using Fixation Buffer (Cat. No. 420801, Biolegend, USA). After washing twice with diluted Intracellular Staining Permeabilization Wash Buffer

(166) HIF regulates multiple translated endogenous retroviruses: Implications for cancer immunotherapyCellApril 3, 2025Qinqin Jiang, David A. Braun, Karl R. Clauser, Vijyendra Ramesh, Nitin H. Shirole, Joseph E. Duke-Cohan, Nancy Nabilsi, Nicholas J. Kramer, Cleo Forman, Isabelle E. Lippincott, et al.450×g for 5 min at RT. The cell pellets were next resuspended in 500 μL of fixation buffer (Biolegend 420801) and analyzed at the Dana-Farber Cancer Institute Flow Cytometry Core Facility using BD LSRFortessa

(167) The FBXO45–GEF-H1 Axis Controls Germinal Center Formation and B-cell LymphomagenesisCancer DiscoveryApril 2, 2025Anagh A. Sahasrabuddhe, Xiaofei Chen, Kaiyu Ma, Rui Wu, Huan-Chang Liang, Richa Kapoor, Rishi R. Chhipa, Ozlem Onder, Courtney McFetridge, John S. Van Arnam, et al.were washed with cell staining buffer and subsequently fixed using fixation buffer (BioLegend, Cat. # 420801) for 20 minutes at room temperature in the dark. Cells were washed and resuspended in cell staining

(168) Functional profiling of p53 and RB cell cycle regulatory proficiency suggests mechanism-driven molecular stratification in endometrial carcinomaCancer Research CommunicationsApril 1, 2025Zelei Yang, Saie Mogre, Hyeji Jun, Ruiyang He, Sneha Ghosh Chaudhary, Upendra Raj. Bhattarai, Shannan J. Ho Sui, Ursula A. Matulonis, Suzan Lazo, Aniket Shetty, et al.were then washed in Cell Staining Buffer, and were then incubated in Fixation Buffer (BioLegend Cat. #420801) for 20 minutes at room temperature in the dark. If the cells were from a 45 minute or 4 hour timepoint,

(169) Functional Profiling of p53 and RB Cell Cycle Regulatory Proficiency Suggests Mechanism-Driven Molecular Stratification in Endometrial CarcinomaCancer Res CommunApril 1, 2025Zelei Yang, Saie Mogre, Hyeji Jun, Ruiyang He, Sneha Ghosh Chaudhary, Upendra Raj Bhattarai, Shannan J. Ho Sui, Ursula A. Matulonis, Suzan Lazo, Aniket Shetty, et al.were then washed in Cell Staining Buffer, and were then incubated in Fixation Buffer (BioLegend Cat. #420801) for 20 minutes at room temperature in the dark. If the cells were from a 45 minute or 4 hour timepoint,

(170) Targeting legumain-mediated cell-cell interaction sensitizes glioblastoma to immunotherapy in preclinical modelsJ Clin InvestMarch 25, 2025Lizhi Pang, Songlin Guo, Yuyun Huang, Fatima Khan, Yang Liu, Fei Zhou, Justin D. Lathia, Peiwen Chenwere pelleted by centrifuging at 300g for 5 minutes and resuspended in the fixation buffer (BioLegend, 420801) overnight at 4°C. A BD FACSymphony or BD LSRFortessa flow cytometer was used to analyze IF intensity

(171) Ancient genomic linkage of α-globin and Nprl3 couples metabolism with erythropoiesisNat CommunMarch 24, 2025Alexandra E. Preston, Joe N. Frost, Megan R. Teh, Mohsin Badat, Andrew E. Armitage, Ruggiero Norfo, Sarah K. Wideman, Muhammad Hanifi, Natasha White, Noémi BA. Roy, et al.antigens of interest, cells were fixed for 20 min in 150 μl 4% paraformaldehyde (BioLegend; Cat. No., 420801) at 4 °C in darkness before a 30-min permeabilisation step (eBioscience; Cat. No. 008333). Staining

(172) The m6A Reader HNRNPC Is a Key Regulator in DSS-Induced Colitis by Modulating Macrophage PhenotypeiScienceMarch 21, 2025Xiaohui Fang, Yu Zhang, Ziliang Ke, Yang Zhang, Yiken Lin, Yibo Huang, Jianhua Zhou, Huiting Su, Jun Xu, Yulan LiuDNase I,Sigma Aldrich,Cat#10104159001 collagenase IV,Sigma Aldrich,Cat# C4-22 fixation buffer,Biolegend,Cat#420801 permeabilization wash buffer,Biolegend,Cat#421002 Mycoplasma Elimination Reagent,Yeasen,Cat#40607ES03 Actinomycin D

(173) An mRNA Vaccine for Herpes Zoster and Its Efficacy Evaluation in Naïve/Primed Murine ModelsVaccines (Basel)March 19, 2025Linglei Jiang, Wenshuo Zhou, Fei Liu, Wenhui Li, Yan Xu, Zhenwei Liang, Man Cao, Li Hou, Pengxuan Liu, Feifei Wu, et al.BV605 anti-Rat CD8 (B.D., 740370) at 4 °C for 30 min. Cells were fixed with Fixation Buffer (BioLegend, 420801) at room temperature for 20 min and washed with PBS (Adamas life, C8020). Cells were resuspended with

(174) An epitope-directed mRNA vaccine inhibits tumor metastasis through the blockade of MICA/B α1/2 sheddingCell Reports MedicineMarch 18, 2025Rui Wang, Jingni Wu, Yifeng Lin, Yufei Xiao, Bin Yang, Sheng Yao, Tianhui Pan, Zhixuan Fu, Shuyu Li, Caihua Wang, et al.(BioLegend, 420601) at 37°C in the presence of 5% CO2 for 6 h, fixed with Fixation Buffer (BioLegend, 420801) at room temperature for 20 min, permeabilized with 1× Intracellular Staining Perm Wash Buffer (BioLegend,

(175) Single-cell and chromatin accessibility profiling reveals regulatory programs of pathogenic Th2 cells in allergic asthmaNat CommunMarch 15, 2025Matarr Khan, Marlis Alteneder, Wolfgang Reiter, Thomas Krausgruber, Lina Dobnikar, Moritz Madern, Monika Waldherr, Christoph Bock, Markus Hartl, Wilfried Ellmeier, et al.requiring intracellular cytokine staining, cells were fixed with 100 µl fixation buffer (BioLegend, 420801) for 15 min, then washed with FACS buffer and permeabilised with 1X permeabilization buffer (BioLegend,

(176) The endogenous antigen-specific CD8+ T cell repertoire is composed of unbiased and biased clonotypes with differential fate commitmentsImmunityMarch 11, 2025Leena Abdullah, Francesco E. Emiliani, Chinmay M. Vaidya, Hannah Stuart, Shawn C. Musial, Fred W. Kolling, Joshua J. Obar, Pamela C. Rosato, Margaret E. Ackerman, Li Song, et al.stains for 30mins at RT followed by fixation for 10mins at RT with fixation buffer (BioLegend, Cat#420801). Flow cytometry was performed on Cytek Aurora (Cytek Biosciences) and the data was analyzed using FlowJo™

(177) Interleukin-16 enhances anti-tumor immune responses by establishing a Th1 cell-macrophage crosstalk through reprogramming glutamine metabolism in miceNature communicationsMarch 10, 2025Wen Z, Liu T, Xu X, Acharya N, Shen Z, Lu Y, Xu J, Guo K, Shen S, Zhao Y, et al.For intracellular staining, cells after surface staining were fixed in Fixation Buffer (Biolegend, #420801) at room temperature for 30 min. Thereafter, cells were permeabilized and stained in Intracellular Staining

(178) BT1549 coordinates the in vitro IL-10 inducing activity of Bacteroides thetaiotaomicronMicrobiology SpectrumMarch 4, 2025Morgan J. Engelhart, Orion D. Brock, Jessica M. Till, Robert W. P. Glowacki, Jason W. Cantwell, David J. Clarke, Darryl A. Wesener, Philip P. Aherntimes with PBS/0.1% BSA and fixed overnight using a paraformaldehyde-based fixative (BioLegend, Cat. #420801). Fixative was washed off using PBS/0.1% BSA and cells were resuspended in PBS/BSA for acquisition on

(179) A metabolic synthetic lethality of phosphoinositide 3-kinase-driven cancerNat CommunMarch 4, 2025Guillaume P. Andrieu, Mathieu Simonin, Aurélie Cabannes-Hamy, Etienne Lengliné, Ambroise Marçais, Alexandre Théron, Grégoire Huré, Jérome Doss, Ivan Nemazanyy, Marie Émilie Dourthe, et al.(BioLegend) on ice for 15 min in ice-cold PBS and washed, fixed for 10 min at 4 °C and (Fixation Buffer #420801, BioLegend) and washed, then permeabilized 30 min at 4 °C (PermBuffer III®, BD Biosciences). After two

(180) UNC119 regulates T-cell receptor signalling in primary T cells and T acute lymphocytic leukaemiaLife science allianceMarch 1, 2025Samarakoon Y, Yelland T, Garcia-Gonzalez E, da Silva Justo Junior A, Mahmood M, Manoharan A, Patterson S, Serafin V, Gammage PA, Marmiroli S, et al.20 min at 4°C. Cells were then washed and either acquired directly or fixed in fixation buffer (Cat #420801; BioLegend), and kept at 4°C until acquisition by flow cytometry. For intracellular staining, samples

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Fixation Buffer from BioLegend

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Fixation Buffer from BioLegend

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