Fixation Buffer from BioLegend

Product Specs
ItemFixation Buffer
CompanyBioLegend
Price $59.00Supplier Page View Company Product Page
Catalog Number420801
ApplicationsICFC - Quality tested, ICC
Quantity100 mL

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(161) The m6A Reader HNRNPC Is a Key Regulator in DSS-Induced Colitis by Modulating Macrophage PhenotypeiScienceMarch 21, 2025Xiaohui Fang, Yu Zhang, Ziliang Ke, Yang Zhang, Yiken Lin, Yibo Huang, Jianhua Zhou, Huiting Su, Jun Xu, Yulan LiuDNase I,Sigma Aldrich,Cat#10104159001 collagenase IV,Sigma Aldrich,Cat# C4-22 fixation buffer,Biolegend,Cat#420801 permeabilization wash buffer,Biolegend,Cat#421002 Mycoplasma Elimination Reagent,Yeasen,Cat#40607ES03 Actinomycin D

(162) An mRNA Vaccine for Herpes Zoster and Its Efficacy Evaluation in Naïve/Primed Murine ModelsVaccines (Basel)March 19, 2025Linglei Jiang, Wenshuo Zhou, Fei Liu, Wenhui Li, Yan Xu, Zhenwei Liang, Man Cao, Li Hou, Pengxuan Liu, Feifei Wu, et al.BV605 anti-Rat CD8 (B.D., 740370) at 4 °C for 30 min. Cells were fixed with Fixation Buffer (BioLegend, 420801) at room temperature for 20 min and washed with PBS (Adamas life, C8020). Cells were resuspended with

(163) An epitope-directed mRNA vaccine inhibits tumor metastasis through the blockade of MICA/B α1/2 sheddingCell Reports MedicineMarch 18, 2025Rui Wang, Jingni Wu, Yifeng Lin, Yufei Xiao, Bin Yang, Sheng Yao, Tianhui Pan, Zhixuan Fu, Shuyu Li, Caihua Wang, et al.(BioLegend, 420601) at 37°C in the presence of 5% CO2 for 6 h, fixed with Fixation Buffer (BioLegend, 420801) at room temperature for 20 min, permeabilized with 1× Intracellular Staining Perm Wash Buffer (BioLegend,

(164) Single-cell and chromatin accessibility profiling reveals regulatory programs of pathogenic Th2 cells in allergic asthmaNat CommunMarch 15, 2025Matarr Khan, Marlis Alteneder, Wolfgang Reiter, Thomas Krausgruber, Lina Dobnikar, Moritz Madern, Monika Waldherr, Christoph Bock, Markus Hartl, Wilfried Ellmeier, et al.requiring intracellular cytokine staining, cells were fixed with 100 µl fixation buffer (BioLegend, 420801) for 15 min, then washed with FACS buffer and permeabilised with 1X permeabilization buffer (BioLegend,

(165) The endogenous antigen-specific CD8+ T cell repertoire is composed of unbiased and biased clonotypes with differential fate commitmentsImmunityMarch 11, 2025Leena Abdullah, Francesco E. Emiliani, Chinmay M. Vaidya, Hannah Stuart, Shawn C. Musial, Fred W. Kolling, Joshua J. Obar, Pamela C. Rosato, Margaret E. Ackerman, Li Song, et al.stains for 30mins at RT followed by fixation for 10mins at RT with fixation buffer (BioLegend, Cat#420801). Flow cytometry was performed on Cytek Aurora (Cytek Biosciences) and the data was analyzed using FlowJo™

(166) Interleukin-16 enhances anti-tumor immune responses by establishing a Th1 cell-macrophage crosstalk through reprogramming glutamine metabolism in miceNature communicationsMarch 10, 2025Wen Z, Liu T, Xu X, Acharya N, Shen Z, Lu Y, Xu J, Guo K, Shen S, Zhao Y, et al.For intracellular staining, cells after surface staining were fixed in Fixation Buffer (Biolegend, #420801) at room temperature for 30 min. Thereafter, cells were permeabilized and stained in Intracellular Staining

(167) A metabolic synthetic lethality of phosphoinositide 3-kinase-driven cancerNat CommunMarch 4, 2025Guillaume P. Andrieu, Mathieu Simonin, Aurélie Cabannes-Hamy, Etienne Lengliné, Ambroise Marçais, Alexandre Théron, Grégoire Huré, Jérome Doss, Ivan Nemazanyy, Marie Émilie Dourthe, et al.(BioLegend) on ice for 15 min in ice-cold PBS and washed, fixed for 10 min at 4 °C and (Fixation Buffer #420801, BioLegend) and washed, then permeabilized 30 min at 4 °C (PermBuffer III®, BD Biosciences). After two

(168) BT1549 coordinates the in vitro IL-10 inducing activity of Bacteroides thetaiotaomicronMicrobiology SpectrumMarch 4, 2025Morgan J. Engelhart, Orion D. Brock, Jessica M. Till, Robert W. P. Glowacki, Jason W. Cantwell, David J. Clarke, Darryl A. Wesener, Philip P. Aherntimes with PBS/0.1% BSA and fixed overnight using a paraformaldehyde-based fixative (BioLegend, Cat. #420801). Fixative was washed off using PBS/0.1% BSA and cells were resuspended in PBS/BSA for acquisition on

(169) UNC119 regulates T-cell receptor signalling in primary T cells and T acute lymphocytic leukaemiaLife science allianceMarch 1, 2025Samarakoon Y, Yelland T, Garcia-Gonzalez E, da Silva Justo Junior A, Mahmood M, Manoharan A, Patterson S, Serafin V, Gammage PA, Marmiroli S, et al.20 min at 4°C. Cells were then washed and either acquired directly or fixed in fixation buffer (Cat #420801; BioLegend), and kept at 4°C until acquisition by flow cytometry. For intracellular staining, samples

(170) Dual functional POGases from bacteria encompassing broader O-glycanase and adhesin activitiesNat CommunFebruary 25, 2025Linjiao Zhou, Uriel Ortega-Rodriguez, Matthew J. Flores, Yasuyuki Matsumoto, John Q. Bettinger, Wells W. Wu, Yaqin Zhang, Su-Ryun Kim, Thomas G. Biel, Jordan D. Pritts, et al.pan-neuraminidase (Cat# P0720L, New England Biolabs) in PBS for 2 h at 37 °C, then fixed with fixation buffer (Cat# 420801, BioLegend) for 10 min at RT. After blocking with 5% (w/v) BSA (Cat# A30075, Fraction V, Research Product

(171) Relationship Between Iron Deficiency and Severity of Tuberculosis: Influence on T Cell SubsetsiScienceFebruary 21, 2025Zheyue Wang, Zhenpeng Guo, Qiang Zhang, Chenchen Yang, Xinling Shi, Qin Wen, Yuan Xue, Zhixin Zhang, Jianming WangHuman TruStain FcX,Biolegend,Cat# 422302 Brilliant Stain Buffer,BD,Cat# 563794 Fixation Buffer,Biolegend,Cat# 420801 Intracellular Staining Perm Wash Buffer,Biolegend,Cat# 421002 ESAT6-peptide-1:MTEQQWNFAGIEAAA,Sangon,NA ESAT6-peptide-2:QWNFAGIEAAASAIQ,Sangon,NA ESAT6-peptide-3:AGIEAAASAIQGNVT,Sangon,NA ESAT6-peptide-4:AAASAIQGNVTSIHS,Sangon,NA ESAT6-peptide-5:AIQGNVTSIHSLLDE,Sangon,NA ESAT6-peptide-6:NVTSIHSLLDEGKQS,Sangon,NA ESAT6-peptide-7:IHSLLDEGKQSLTKL,Sangon,NA ESAT6-peptide-8:LDEGKQSLTKLAAAW,Sangon,NA ESAT6-peptide-9:KQSLTKLAAAWGGSG,Sangon,NA ESAT6-peptide-10:TKLAAAWGGSGSEAY,Sangon,NA ESAT6-peptide-11:AAWGGSGSEAYQGVQ,Sangon,NA ESAT6-peptide-12:GSGSEAYQGVQQKWD,Sangon,NA ESAT6-peptide-13:EAYQGVQQKWDATAT,Sangon,NA ESAT6-peptide-14:GVQQKWDATATELNN,Sangon,NA ESAT6-peptide-15:KWDATATELNNALQ,Sangon,NA CFP10-peptide-1:MAEMKTDAATLAQEA,Sangon,NA CFP10-peptide-2:KTDAATLAQEAGNFE,Sangon,NA CFP10-peptide-3:ATLAQEAGNFERISG,Sangon,NA CFP10-peptide-4:QEAGNFERISGDLKT,Sangon,NA CFP10-peptide-5:NFERISGDLKTQIDQ,Sangon,NA CFP10-peptide-6:ISGDLKTQIDQVEST,Sangon,NA CFP10-peptide-7:LKTQIDQVESTAGSL,Sangon,NA CFP10-peptide-8:IDQVESTAGSLQGQW,Sangon,NA CFP10-peptide-9:ESTAGSLQGQWRGAA,Sangon,NA CFP10-peptide-10:GSLQGQWRGAAGTAA,Sangon,NA CFP10-peptide-11:GQWRGAAGTAAQAAV,Sangon,NA CFP10-peptide-12:GAAGTAAQAAVVRFQ,Sangon,NA CFP10-peptide-13:TAAQAAVVRFQEAAN,Sangon,NA CFP10-peptide-14:AAVVRFQEAANKQKQ,Sangon,NA CFP10-peptide-15:RFQEAANKQKQELDE,Sangon,NA CFP10-peptide-16:AANKQKQELDEISTN,Sangon,NA CFP10-peptide-17:QKQELDEISTNIRQA,Sangon,NA CFP10-peptide-18:LDEISTNIRQAGVQY,Sangon,NA CFP10-peptide-19:STNIRQAGVQYSRAD,Sangon,NA CFP10-peptide-20:RQAGVQYSRADEEQQ,Sangon,NA CFP10-peptide-21:VQYSRADEEQQQALS,Sangon,NA CFP10-peptide-22:RADEEQQQALSSQMGF,Sangon,NA ,, Critical

(172) Recognition of MR1-antigen complexes by TCR Vγ9Vδ2Frontiers in ImmunologyFebruary 18, 2025José Pedro Loureiro, Alessandro Vacchini, Giuliano Berloffa, Jan Devan, Verena Schaefer, Vladimir Nosi, Rodrigo Colombo, Aisha Beshirova, Giulia Montanelli, Benedikt Meyer, et al.staining. For intracellular staining, stained cells were fixed with a Fixation Buffer (Biolegend, #420801) for 15 min at RT. Cells were permeabilized using Intracellular Staining Permeabilization Wash Buffer

(173) Nutrient-driven histone code determines exhausted CD8 + T cell fatesScienceFebruary 7, 2025Shixin Ma, Michael S. Dahabieh, Thomas H. Mann, Steven Zhao, Bryan McDonald, Won-Suk Song, H. Kay Chung, Yagmur Farsakoglu, Lizmarie Garcia-Rivera, Filipe Araujo Hoffmann, et al.Intracellular staining of cytokines and effector molecules was performed using fixation buffer (BioLegend; 420801) after stimulation with ionomycine (Sigma-Aldrich) and phorbol 12-myristate 13-acetate (PMA; Sigma-Aldrich)

(174) Chiral polypeptide hydrogels regulating local immune microenvironment and anti-tumor immune responseNature CommunicationsJanuary 31, 2025Junfeng Ding, Tianran Wang, Zhiqiang Lin, Zhenyu Li, Jiaxuan Yang, Fujiang Li, Yan Rong, Xuesi Chen, Chaoliang Heantibodies for 30 min on ice. Subsequently, the cells were fixed by fixation buffer (Biolegend Cat. 420801) and washed twice by intracellular staining perm wash buffer (Biolegend Cat. 421002). Cells were then

(175) Rescue of in vitro models of CSF1R-related adult-onset leukodystrophy by iluzanebart: mechanisms and therapeutic implications of TREM2 agonismJournal of neuroinflammationJanuary 31, 2025Larson KC, Gergits FW, Renoux AJ, Weisman EJ, Dejanovic B, Huang L, Pandya B, McLaren DG, Lynch BA, Fisher R, et al.transcardially perfused with cold HBSS followed by perfusion with cold fixation buffer (Biolegend #420801). Whole brains, excluding cerebellum, were removed and placed in paraformaldehyde quench solution (HBSS,

(176) Pathogenic role for CD101-negative neutrophils in the type I interferon-mediated immunopathogenesis of tuberculosisCell reportsJanuary 28, 2025Saqib M, Das S, Nafiz TN, McDonough E, Sankar P, Mishra LK, Zhang X, Cai Y, Subbian S, Mishra BB.1119,Sigma-Aldrich,Cat#111916X100mL Fixable viability dye; eFluor 780 conjugated,eBioscience,Cat# 65-0864-14 Fixation buffer,Biolegend,Cat# 420801 T-PER buffer,ThermoFisher,Cat#78510 ,, Critical commercial assays,Critical commercial assays,Critical

(177) The TLR7/9 adaptors TASL and TASL2 mediate IRF5-dependent antiviral responses and autoimmunity in mouseNature communicationsJanuary 24, 2025Drobek A, Bernaleau L, Delacrétaz M, Calderon Copete S, Royer-Chardon C, Longepierre M, Monguió-Tortajada M, Korzeniowski J, Rotman S, Marquis J, et al.For the phospho-specific staining, cells were fixed and permeabilized using Fix/perm kit (Biolegend, 420801, 421002). Samples were analysed on BD LSRII or Cytoflex S (Beckman Coulter) flow cytometer and data

(178) The direct and indirect inhibition of proinflammatory adipose tissue macrophages by acarbose in diet-induced obesityCell reports. MedicineJanuary 21, 2025Li X, Zheng S, Xu H, Zhang Z, Han X, Wei Y, Jin H, Du X, Xu H, Li M, et al.00-5123-43 Fixation/Permeabilization Diluent,Thermo Fisher Scientific,Cat#: 00-5223-56 Fixation Buffer,BioLegend,Cat#: 420801 Dimethyl sulfoxide (DMSO),Sigma-Aldrich,Cat#: 67-68-5 "Granulocyte-MacrophageColony-Stimulating Factor,

(179) The FBXO45–GEF-H1 Axis Controls Germinal Center Formation and B-cell LymphomagenesisCancer DiscovJanuary 17, 2025Anagh A. Sahasrabuddhe, Xiaofei Chen, Kaiyu Ma, Rui Wu, Huan-Chang Liang, Richa Kapoor, Rishi R. Chhipa, Ozlem Onder, Courtney McFetridge, John S. Van Arnam, et al.were washed with cell staining buffer and subsequently fixed using fixation buffer (BioLegend, Cat. # 420801) for 20 minutes at room temperature in the dark. Cells were washed and resuspended in cell staining

(180) Mannose metabolism reshapes T cell differentiation to enhance anti-tumor immunityCancer CellJanuary 13, 2025Yajing Qiu, Yapeng Su, Ermei Xie, Hongcheng Cheng, Jing Du, Yue Xu, Xiaoli Pan, Zhe Wang, Daniel G. Chen, Hong Zhu, et al.assays Ficoll Paque PLUS,Cytiva,Cat# 17144002 RNAiso Plus,Takara,Cat# 9108 Fixation Buffer,BioLegend,Cat# 420801 Intracellular Staining Permeabilization Wash Buffer,BioLegend,Cat# 421002 MojoSort™ Mouse CD8 Naïve T Cell Isolation Kit

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Fixation Buffer from BioLegend

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Fixation Buffer from BioLegend

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