Fixation Buffer from BioLegend

Product Specs
ItemFixation Buffer
CompanyBioLegend
Price $59.00Supplier Page View Company Product Page
Catalog Number420801
ApplicationsICFC,ICC
Quantity100ML

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(121) Optimization of the Blocking and Signal Preservation Protocol in High‐Parameter Flow CytometryCurrent ProtocolsSeptember 25, 2025Oliver T. Burton, James Dooley, Adrian Listonsolution (BioLegend, cat No. 420601; various sources are acceptable)Fixation Buffer (BioLegend, cat. no. 420801), Cyto‐Fast Fix/Perm Buffer Set (BioLegend, cat. no. 426803) or IC Fixation Buffer (Thermo Fisher, cat.

(122) Engineered T cells stimulate dendritic cell recruitment and antigen spreading for potent anti-tumor immunityCell Reports MedicineSeptember 16, 2025Zhen Xiao, Jiajia Wang, Shidian He, Lin Wang, Jingxing Yang, Wenhui Li, Kaili Ma, Yabo Zhou, Xiaowei Liu, Shiyou Wang, et al.System,Vazyme,Cat#DD1204-01 Chromium Single Cell 30 v2 reagent kit,10× Genomics,N/A Fixation Buffer,BioLegend,Cat#420801 Intracellular Staining Permeabilization Wash Buffer,BioLegend,Cat#421002 eBioscience™ Permeabilization

(123) Surface-Engineered HA-PEG-ICG/PLGA Nanoprobes with Vessels Targeting for Lymphatic System VisualizationACS Applied Bio MaterialsSeptember 15, 2025Hao-Han Chiang, Yu-Teng Chang, Wei-Ren Huang, Min-Xuan Cai, Chin-Hsing Feng, Jia-Ning Syu, Chih-Sheng Lai, Yi-Hsin ChienFixation was performed in the dark for 20 min at room temperature using a fixation buffer (BioLegend, Cat# 420801). To permeabilize the cells, 0.1% Triton X-100 in PBS was applied for 3–5 min, followed by 2–3 washes

(124) Epigenetic silencing of interleukin-10 by host-derived oxidized phospholipids supports a lethal inflammatory response to infectionsImmunitySeptember 9, 2025Marco Di Gioia, Valentina Poli, Piao J. Tan, Roberto Spreafico, Anne Chu, Alex G. Cuenca, Zhongyang Wu, Mehdi Benamar, Laura Pandolfi, Federica Meloni, et al.107622) and BV785 CD11c (BioLegend, Cat# 117336). Cells were fixed (Fixation buffer, BioLeged, Cat# 420801), permeabilized (Intracellular Staining Permeabilization Wash Buffer, BioLegend, Cat# 421002) and incubated

(125) Bronchial pyroptosis promotes Th17 inflammation in steroid-insensitive asthma mouseInnate ImmunAugust 28, 2025Yun Lin, Jianhua Yin, Xia Yang, Jianghong Wei, Yaxi Liang, Chengfeng Zhou, Dongfang Zou, Shuyuan Chucocktail (00-4975-93, eBioscience, CA, USA) at 37°C for 5 h, and then incubated with IC fixation buffer (420801, Biolegend) for 30 min without light according to manufacturer's protocol. The cells were stained with

(126) The bispecific innate cell engager AFM28 eliminates CD123(+) leukemic stem and progenitor cells in AML and MDSNature communicationsAugust 21, 2025Schmitt N, Siegler JJ, Beck A, Müller T, Kozlowska I, Sarlang S, Reusch U, Knackmuss S, Medina-Echeverz J, Koch J, et al.non-targeting RSV/CD16A, followed by fixation and cell lysis (Fixation buffer, BioLegend, cat. no. 420801; True-Phos Perm Buffer, BioLegend, cat. no. 425401). Analyses were performed using BD FACSCelesta (Becton Dickinson

(127) Isolation and Ex Vivo Testing of CD8+ T-Cell Division and Activation Using Mouse SplenocytesBio ProtocAugust 20, 2025Melissa Dolan, Yuhao Shi, Amber McKenery, Stephanie Tzetzo, Kohei Chida, Kazuaki Takabe, Scott I. Abrams, John M.L. Ebos1) 13. Flow cytometry staining antibodies (see Table 1) 14. Fixation buffer (BioLegend, catalog number: 420801) 15. Intracellular staining permeabilization wash buffer (10×) (BioLegend, catalog number: 421002) 16.

(128) Isolation and Ex Vivo Testing of CD8+ T-Cell Division and Activation Using Mouse SplenocytesBio ProtocAugust 20, 2025Melissa Dolan, Yuhao Shi, Amber McKenery, Stephanie Tzetzo, Kohei Chida, Kazuaki Takabe, Scott I. Abrams, John M.L. Ebos1) 13. Flow cytometry staining antibodies (see Table 1) 14. Fixation buffer (BioLegend, catalog number: 420801) 15. Intracellular staining permeabilization wash buffer (10×) (BioLegend, catalog number: 421002) 16.

(129) BRD9 inhibition overcomes oncolytic virus therapy resistance in glioblastomaCell Reports MedicineAugust 19, 2025Chen Guo, Zhilin Long, Peng Lin, Yinan Shen, Yiye Zhong, Jingjing Qian, Jichuan Yu, Weixi Zhao, Fuyi Liu, Yiling Ma, et al.staining at 4°C for 45 min and the intracellular staining was performed with fixation buffer (Biolegend, #420801) and intracellular staining buffer (Biolegend, #421002) according to the manufacturer’s instructions.

(130) Rewired type I IFN signaling is linked to age-dependent differences in COVID-19Cell Reports MedicineAugust 19, 2025Lev Petrov, Sophia Brumhard, Sebastian Wisniewski, Philipp Georg, David Hillus, Anna Hiller, Rosario Astaburuaga-García, Nils Blüthgen, Emanuel Wyler, Katrin Vogt, et al.(37°C) commercially available fixation buffer, according to manufacturer`s protocol (BioLegend Cat 420801). After 15 min of fixation at 37°C, cells were washed in FACS buffer (2 times, 500xg, 5 min, at room

(131) Early-life human CD8 + T cells exhibit rapid, short-lived effector responses and a unique transcription factor landscapeProceedings of the National Academy of SciencesAugust 5, 2025Nina N. Brodsky, Monisha Chakder, Dinesh Babu Uthaya Kumar, Anis Barmada, Julia Wang, Weihong Gu, Oluwabunmi Olaloye, Anjali Ramaswamy, Aanchal Wats, Liza Konnikova, et al.albumin--BSA, 2 mL EDTA, 2mM, pH 8.5) and then fixed with 4% paraformaldehyde (BioLegend, catalog no. 420801). Cells were then permeabilized using perm/wash buffer (BioLegend, catalog no. 421002). Intracellular

(132) Spermidine potentiates anti-tumor immune responses and immunotherapy sensitivity in breast cancerJournal of CancerJuly 28, 2025Xinyu Yang, Yaxin Feng, Ruxin Wang, Xin Zeng, Boying Gao, Penghan Huang, Huiping Chen, Wenfeng Zengwithout light exposure. For intracellular molecules, cells were fixed using fixation buffer (Biolegend, 420801) for 15min at room temperature, followed by two washes with perm/wash buffer (BioLegend, 421002). Subsequently,

(133) Dysregulation of decidual NK cell proliferation by impaired decidual cells: a potential contributor to excessive trophoblast invasion in placenta accreta spectrumFrontiers in Cell and Developmental BiologyJuly 21, 2025You-Zhen Liu, Hsin-Hung Lin, Meng-Shiue Wu, Jin-Chung Shih, Thai-Yen Lingperiod, cells were washed with PBS (pH 7.4) and fixed with 4% paraformaldehyde solution (BioLegend, 420801) in PBS for 10 min at room temperature. After fixation, cells underwent three PBS washes. They were

(134) Erythroblasts Promote the Development of a Suppressive Lymphocyte Phenotype via Treg Induction and PD1 Upregulation on the Surfaces of B-Cells: A Study on the Subpopulation-Specific Features of ErythroblastsCurr Issues Mol BiolJuly 15, 2025Kirill Nazarov, Roman Perik-Zavodskii, Julia Shevchenko, Sergey Sennikovfixation/permeabilization buffers. Next, the cells were fixed in 0.5 mL per sample of Fix Buffer (cat#420801, BioLegend, San Diego, CA, USA) containing 4% formaldehyde for 20 min. After fixation and washing out

(135) IFNγ augments TKI efficacy by alleviating protein unfolding stress to promote GSDME-mediated pyroptosis in hepatocellular carcinomaCell Death DisJuly 11, 2025Xiaoxiao Li, Fujia Lu, Jie Zhou, Xiong Li, Yan Li, Weijie Ye, Jing Li, Liguo Yang, Shi Tang, Yuhan Zhou, et al.for 20 min. Then, cells were washed and permeabilized in 200 μL Fixation/perm solution (Biolegend, 420801) at room temperature for 20 min, washed with the Perm/Wash buffer (Biolegend, 421002), and then stained

(136) Transferrin receptor 1 nuclear translocation facilitates tumor progression via p53-mediated chromatin interactions and genome-wide alterationsSignal Transduct Target TherJuly 8, 2025Yaxin Hou, Guoheng Tang, Qizhi Wang, Meng Zhou, Ran Xu, Xuehui Chen, Guizhi Shi, Zhuoran Wang, Xiyun Yan, Jie Zhuang, et al.cells per well). Subsequently, the cells were harvested, fixed with the Fixation Buffer (Biolegend, 420801), permeabilized with the Intracellular Staining Perm Wash Buffer (Biolegend, 421002), and incubated

(137) Glycoprotein NMB mediates bidirectional GSC-TAM interactions to promote tumor progressionJCI InsightJuly 8, 2025Yang Liu, Lizhi Pang, Fatima Khan, Junyan Wu, Fei Zhou, Craig Horbinski, Shideng Bao, Jennifer S. Yu, Justin D. Lathia, Peiwen Chensurface marker staining. After washing with PBS, cells were incubated with fixation buffer (BioLegend, 420801) for 20 minutes. Cells were then permeabilized by permeabilization buffer (0.1% Triton X-100 in PBS).

(138) Bioengineered immunocompetent preclinical trial-on-chip tool enables screening of CAR T cell therapy for leukaemiaNature Biomedical EngineeringJuly 1, 2025Chao Ma, Huishu Wang, Lunan Liu, Ruiqi Chen, Nandana Mukherjee, Jie Tong, Shadab Kazmi, Xiangyi Fang, Matthew T. Witkowski, Iannis Aifantis, et al.of ECMs during the 7-day culture, leukaemia chips were fixed with fixation buffer (catalogue number 420801, BioLegend) each day and stained with DyLight488-Laminin (catalogue number PA5-22901, Thermo Fisher Scientific

(139) Heterogeneity of IL-15-expressing mesenchymal stromal cells controls natural killer cell development and immune cell homeostasisNat CommunJuly 1, 2025Carmen Stecher, Romana Bischl, Anna Schmid-Böse, Stefanie Ferstl, Elisabeth Potzmann, Magdalena Frank, Nina Braun, Matthias Farlik, Richard A. Flavell, Dietmar Herndler-BrandstetterBrefeldin A (BioLegend, Cat# 420601) for 3 hours and then fixed in Fixation Buffer (BioLegend, Cat# 420801) prior to permeabilization using Permeabilization Wash Buffer and intracellular cytokine staining. Elisa ACK-lysed

(140) KMT2D coordinates antiviral CD4+ T cell responses through opposing effects on T follicular helper and cytotoxic gene expressionCell ReportsJune 24, 2025Jonathan A. Cohen, Ashlyn A. Buzzelli, Gabrielle Quickstad, Lixin Yang, Ichiro Misumi, Karl B. Shpargel, Jason K. WhitmireBovine Serum,VWR,Cat# 97068-085 Ficoll-Paque PLUS,Cytiva,Cat# 17144002 Fixation Buffer,Biolegend,Cat# 420801 FoxP3 Transcription Factor Staining Kit,eBioscience,Cat# 00-5523-00 KAPA pure SPRI beads,Rocke,Cat#

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Fixation Buffer from BioLegend

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Fixation Buffer from BioLegend

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