Fixation Buffer from BioLegend

Product Specs
ItemFixation Buffer
CompanyBioLegend
Price $59.00Supplier Page View Company Product Page
Catalog Number420801
ApplicationsICFC,ICC
Quantity100ML

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(141) Ultrabright ratiometric Raman-guided epilepsy surgery by intraoperatively visualizing proinflammatory microgliaCell Reports MedicineJune 17, 2025Cong Wang, Zhi Li, Xiao Zhu, Wanbing Sun, Yue Ding, Wenjia Duan, Difei Wang, Yiqing Jiang, Ming Chen, Yuncan Chen, et al.buffer,Biolegend,Cat. #421002 Cell staining buffer,Biolegend,Cat. #420201 Fixation buffer,Biolegend,Cat. #420801 RIPA lysis buffer,Beyotime,Cat. #P0013B QuickBlock™ blocking buffer,Beyotime,Cat. #P0220 18F-DPA714,Huashan

(142) Immunomodulatory Effects of Pulmonarom®: In Vitro Induction of TLR and Cytokine Expression in Human Dendritic CellsPharmaceuticals (Basel)June 13, 2025Juan A. Hernández-Aceves, Sandra Georgina Solano-Gálvez, Arturo A. Wilkins-Rodríguez, José Delgado-Domínguez, Alberto Garcia Lozano, Carlos Cabello-Gutierrez, Lidia Flor Estela Huerta, Gladis Fragoso, Laila Gutiérrez-Kobeh, Rosalino Vázquez-Lópezthe cells were stained extracellularly, they were fixed with a Fixation Buffer reagent (Biolgend Cat. 420801) for 20 min at room temperature. Thereafter, they were washed with a wash buffer (Biolgend Cat. 421002),

(143) Protocol for phenotyping and isolation of dendritic cell subsets from blood and lymphoid organs of non-human primates and humans by flow cytometrySTAR ProtocJune 13, 2025Juliette Pons, Margaux Gardet, Florian Meurisse, Francis Relouzat, Olivier Lambotte, Benoit Favier254134 KHCO3,Sigma,Cat# 60339 EDTA tetrasodium salt,Sigma,Cat# E5391 4% PFA fixation buffer,BioLegend,Cat# 420801

(144) Brown adipose tissue secretes OLFM4 to coordinate sensory and sympathetic innervation via Schwann cellsNat CommunJune 4, 2025Mingqiang Lai, Wu Zhou, Wenchong Zou, Lianlian Qiu, Zhaoyu Liang, Wanyi Chen, Yiqing Wang, Bin Guo, Chaoran Zhao, Sheng Zhang, et al.staining for the cell surface antigens, the SVF cells were fixed in Fixation Buffer (BioLegend Cat. No. 420801) in the dark for 20 min at room temperature. The Fixation Buffer was removed by centrifuging the samples

(145) IFN-γ–resistant CD28 CAR T cells demonstrate increased survival, efficacy, and durability in multiple murine tumor modelsScience Translational MedicineJune 4, 2025Stefanie R. Bailey, Hana N. Takei, Giulia Escobar, Michael C. Kann, Amanda A. Bouffard, Tamina Kienka, Valentina M. Supper, Alexander Armstrong, Diego Salas-Benito, Merle K. Phillips, et al.FACS Buffer (PBS + 2% FBS) prior to acquisition. Cells were fixed with Fixation Buffer (BioLegend Cat. 420801) and incubated in Intracellular Staining Permeabilization Wash Buffer (10X) (BioLegend Cat. 421002)

(146) Potentiating the effect of immunotherapy in pancreatic cancer using gas-entrapping materialsBiomaterialsJune 1, 2025Jianling Bi, Emily Witt, Megan K. McGovern, Arielle B. Cafi, Sri Naga Swetha Swetha Tunuguntla, Alicia T. Cotoia, Juan Antonio Raygoza Raygoza Garay, Kyle R. Balk, Lilly Boge, Samual Hatfield, et al.200 μl ice-cold FACS buffer. Stained cells were fixed with True Nuclear Fixation Buffer (Biolegend, 420801) and 1x Tandem Stabilizer (Biolegend, 421802). UltraComp eBeads (Invitrogen, #01–2222-41) were used

(147) Cbl-b inhibitor NX-1607 activates MAPK/ERK signaling pathway and enhances T-cell activationJ Immunother CancerMay 30, 2025Wenting Zhu, Shan Lu, Li Jia, Benjin Liu, Shanshan Song, Xubin Bao, Ting Yu, Yongliang Zhang, Zehong Miao, Jinxue Heµg/mL plate-bound anti-CD28 for 1 hour at 37°C. The cells were then fixed with warm fixation buffer (#420801, BioLegend) for 15 min at 37°C and permeabilized with chill True-Phos Perm Buffer (#425401, BioLegend)

(148) Sulfasalazine combined with anti-IL-1β mAb induces ferroptosis and immune modulation in oral squamous cell carcinomaCell Mol Life SciMay 28, 2025Rui Zhou, Jiaying Zhou, Yuwen Xiong, Kai Su, Changlin Liu, Bin Cheng, Tong Wuthen washed once with FACS buffer. Following the washing step, cells were fixed with Fixation Buffer (420801, BioLegend, USA) for 30 min in the dark at 4 °C and then washed twice with Intracellular Staining Permeabilization

(149) Adoptively transferred macrophages for cancer immunotherapyJ Immunother CancerMay 24, 2025Kyung Soo Park, Alexander P Gottlieb, Morgan E Janes, Supriya Prakash, Neha Kapate, Vineeth Chandran Suja, Lily Li-Wen Wang, Jennifer L Guerriero, Samir Mitragotrifixation, cells were washed two times with staining buffer, resuspended with 100 µL of fixation buffer (420801; BioLegend), and incubated for 10 min at room temperature (RT). After fixation, samples were washed

(150) Celluloepidemiology-A paradigm for quantifying infectious disease dynamics on a population levelSci AdvMay 16, 2025My K. Ha, Anna Postovskaya, Maria Kuznetsova, Pieter Meysman, Vincent Van Deuren, Sabrina Van Ierssel, Hans De Reu, Jolien Schippers, Karin Peeters, Hajar Besbassi, et al.stain for 15 min. Then, they were fixed for 10 min in 0.4% paraformaldehyde (BioLegend, catalog no. 420801) at room temperature. Afterward, they were stained with a panel of 12 fluorescent antibodies (table

(151) Epigenomic landscapes define differential Janus kinases inhibitor sensitivity in IFN-γ-primed human macrophagesiScienceMay 16, 2025Geunho Kwon, Yebin Park, Keunsoo Kang, Kyung-Hyun Park-Min, Kyuho KangInterferon-γ, human (hIFN-γ)",Roche,Cat# 11040596001 Deoxyribonuclease I,Worthington,Cat# LS002004 Fixation buffer,BioLegend,Cat# 420801 True-Phos™ Perm,BioLegend,Cat# 425401 Cell staining buffer,BioLegend,Cat# 420201 Dulbecco’s phosphate

(152) Targeting legumain-mediated cell-cell interaction sensitizes glioblastoma to immunotherapy in preclinical modelsJournal of Clinical InvestigationMay 15, 2025Lizhi Pang, Songlin Guo, Yuyun Huang, Fatima Khan, Yang Liu, Fei Zhou, Justin D. Lathia, Peiwen Chenwere pelleted by centrifuging at 300g for 5 minutes and resuspended in the fixation buffer (BioLegend, 420801) overnight at 4°C. A BD FACSymphony or BD LSRFortessa flow cytometer was used to analyze IF intensity

(153) Malnutrition exacerbates pathogenesis of Lutzomyia longipalpis sand fly-transmitted Leishmania donovaniCommun BiolMay 13, 2025Eva Iniguez, Johannes Doehl, Pedro Cecilio, Tiago Donatelli Serafim, Caroline Percopo, Yvonne Rangel-Gonzalez, Somaditya Dey, Elvia J. Osorio, Patrick Huffcutt, Sofia Roitman, et al.710-Ly6G (clone: 1A; eBioscience, Cat No. 46966882) for 30 min. Cells were fixed (Biolegend, Cat No. 420801) and permeabilized (Biolegend, Cat No. 421002), followed by intracellular staining with APC IL1-β pro-form

(154) Lymph node macrophages drive immune tolerance and resistance to cancer therapy by induction of the immune-regulatory cytokine IL-33Cancer CellMay 12, 2025Sara Lamorte, Rene Quevedo, Robbie Jin, Luke Neufeld, Zhe Qi Liu, M. Teresa Ciudad, Sabelo Lukhele, Jessica Bruce, Shreya Mishra, Xin Zhang, et al."CD14 MicroBeads UltraPure, human",Miltenyi Biotec,Cat#130-118-906 Cytokine staining kit,Biolegend,"Cat#420801, #421002" OCT,VWR,Cat#95057-838 ProLong™ Diamond Antifade Mountant,ThermoFisher,Cat#P36965 FocusClear™,CelExplorer,Cat#FC-101 EasySep™ Biotin Positive Selection Kit II

(155) Comparative evaluation of immunomodulatory cytokines for oncolytic therapy based on a high-efficient platform for oHSV1 reconstructionVirology journalMay 5, 2025Gao Y, Zou Y, Wu C, Tao J, Nie Z, Yan J, Wang P, Huang X.oHSV1s at MOI = 1 for 24 h. Then the cells were harvested and fixed with Fixation Buffer (BioLegend, 420801). After washing with Cell Staining Buffer (BioLegend, 420201), cells were stained with APC anti-mouse H-2Kd/H-2Dd

(156) Therapeutic potential of voltage-dependent potassium channel subtype 1.3 blockade in alleviating macrophage-related renal inflammation and fibrogenesisCell death discoveryMay 5, 2025Li SS, Liang Y, Kong JW, Zhang Q, Qian JR, Yu LX, Liu QF.APC anti-mouse CD206, FcRblock anti-mouse CD16/32 (101319) antibodies, along with fixation buffer (420801) and intracellular staining permeabilization wash buffer (421002) were obtained from BioLegend, Inc.

(157) The Emerging Fungal Pathogen Candida auris Induces IFNγ to Colonize the SkinPLOS PathogensApril 28, 2025Diprasom Das, Shrihari M. Ganesh, Abtar Mishra, Mihai G. Netea, Shankar Thangamani(Cat# 420201), intracellular staining permeabilization wash buffer (Cat#421002), fixation buffer (Cat# 420801), cell activation cocktail (without Brefeldin) (Cat# 423302) was purchased from Biolegend. 3T3-L1 (Cat#

(158) The mechanism of YAP/TAZ transactivation and dual targeting for cancer therapyNat CommunApril 24, 2025Man Yu, Jingning Wang, Xiao Zhang, Haoran Zhang, Chaoqiang Li, Juebei Li, Jiaming Lin, Jie Zheng, Liu Huang, Yan Li, et al.temperature. Then cells were washed and permeabilized in 150 μl Fixation/perm solution (Biolegend, 420801) at 4 °C for 40 min, washed with the Perm/Wash buffer (Biolegend, 421002), and then stained with anti-IFNγ-Bv421(BioLegend,

(159) SIRT2 suppresses aging-associated cGAS activation and protects aged mice from severe COVID-19Cell ReportsApril 22, 2025Marine Barthez, Biyun Xue, Jian Zheng, Yifei Wang, Zehan Song, Wei-Chieh Mu, Chih-ling Wang, Jiayue Guo, Fanghan Yang, Yuze Ma, et al.Biosciences,Cat# 95048 BD Pharm Lyse™ Lysing Buffer,BD Biosciences,Cat# 555899 Fixation Buffer,BioLegend,Cat# 420801 Intracellular Staining Permeabilization Wash Buffer,BioLegend,Cat# 421002 Formaldehyde,Thermo Fisher

(160) Subnanomolar MAS-related G protein-coupled receptor-X2/B2 antagonists with efficacy in human mast cells and disease modelsSignal Transduct Target TherApril 21, 2025Ghazl Al Hamwi, Mohamad Wessam Alnouri, Sven Verdonck, Piotr Leonczak, Shaswati Chaki, Stefan Frischbutter, Pavel Kolkhir, Michaela Matthey, Constantin Kopp, Marek Bednarski, et al.°C. After 5 min of incubation, cells were fixed with 500 µL of 4% fixation buffer (Biolegend, Catalog 420801) for 15 min at room temperature. The reaction was stopped with cold FACS buffer [1X DPBS containing

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Fixation Buffer from BioLegend

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Fixation Buffer from BioLegend

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