Maxwell® RSC 48 Instrument from Promega

Product Specs
ItemMaxwell® RSC 48 Instrument
CompanyPromega
Price Supplier Page
Catalog NumberAS8500
QuantityEA

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(161) Viral Sequencing to Investigate Sources of SARS-CoV-2 Infection in US Healthcare PersonnelClinical Infectious DiseasesSeptember 15, 2021Katarina M Braun, Gage K Moreno, Ashley Buys, Elizabeth D Somsen, Max Bobholz, Molly A Accola, Laura Anderson, William M Rehrauer, David A Baker, Nasia Safdar, et al.Briefly, viral RNA was extracted using the Viral Total Nucleic Acid Purification kit (Promega) on a Maxwell RSC 48 instrument. Complementary DNA was synthesized using SuperScript IV reverse-transcriptase [24, 25]. SARS-CoV-2–specific

(162) Pathogenicity of H9N2 low pathogenic avian influenza viruses of different lineages isolated from live bird markets tested in three animal models: SPF chickens, Korean native chickens, and ducksPoultry ScienceSeptember 1, 2021Soo-Jeong Kye, Min-Ji Park, Na-Young Kim, Yu-Na Lee, Gyeong-Beom Heo, Yoon-Ki Baek, Jae-In Shin, Myoung-Heon Lee, Youn-Jeong Leesuspension (supernatant) was subjected to RNA extraction using the Maxwell RSC simplyRNA Tissue kit and a Maxwell RSC 48 instrument (Promega, Germany). The cycle threshold (Ct) value was used to calculate the viral load after real-time

(163) Acute SARS-CoV-2 infections harbor limited within-host diversity and transmit via tight transmission bottlenecksPLOS PathogensAugust 23, 2021Katarina M. Braun, Gage K. Moreno, Cassia Wagner, Molly A. Accola, William M. Rehrauer, David A. Baker, Katia Koelle, David H. O’Connor, Trevor Bedford, Thomas C. Friedrich, et al.100 μl of VTM using the Viral Total Nucleic Acid Purification kit (Promega, Madison, WI, USA) on a Maxwell RSC 48 instrument and eluted in 50 μL of nuclease-free H2O. Complementary DNA (cDNA) generation and PCR Complementary

(164) Zoonotic Soil-Transmitted Helminths in Free-Roaming Dogs, KiribatiEmerging Infectious DiseasesAugust 1, 2021Patsy A. Zendejas-Heredia, Allison Crawley, Helen Byrnes, Rebecca J. Traub, Vito Colellahttps://www.mpbio.com). After bead-beating and cell lysis, we proceeded with DNA purification in a Maxwell RSC 48 Instrument (Promega). We stored the final eluted sample (100 μL) at −20°C for further analyses. We subjected the

(165) Previous exposure to dengue virus is associated with increased Zika virus burden at the maternal-fetal interface in rhesus macaquesPLOS Neglected Tropical DiseasesJuly 30, 2021Chelsea M. Crooks et al.plasma aliquot using the Viral Total Nucleic Acid Kit (Promega, Madison, WI) on a Maxwell 16 MDx or Maxwell RSC 48 instrument (Promega, Madison, WI). Viral RNA isolation from tissues Tissue samples, cut to 0.5 cm thickness on

(166) African-Lineage Zika Virus Replication Dynamics and Maternal-Fetal Interface Infection in Pregnant Rhesus MacaquesJournal of VirologyJuly 26, 2021Chelsea M. Crooks et al.plasma aliquot using the Viral Total Nucleic Acid kit (Promega, Madison, WI) on a Maxwell 16 MDx or Maxwell RSC 48 instrument (Promega, Madison, WI). Viral RNA isolation from tissues. Tissue samples, cut to a 0.5-cm thickness

(167) Severe Acute Respiratory Syndrome Coronavirus 2 Transmission in Intercollegiate Athletics Not Fully Mitigated With Daily Antigen TestingClinical Infectious DiseasesJuly 15, 2021Gage K Moreno, Katarina M Braun, Ian W Pray, Hannah E Segaloff, Ailam Lim, Keith Poulsen, Jonathan Meiman, James Borcher, Ryan P Westergaard, Michael K Moll, et al.100 μL of viral transport medium using the Viral Total Nucleic Acid Purification kit (Promega) on a Maxwell RSC 48 instrument, according to manufacturer guidelines, and was eluted in 50 μL of nuclease-free water. Library Preparation

(168) Expanding spectrum of “spitzoid” lesions: a small series of 4 cases with MAP2K1 mutationsVirchows ArchivJuly 1, 2021K. G. P. Kerckhoffs, T. Aallali, C. A. Ambarus, V. Sigurdsson, A. M. L. Jansen, W. A. M. Blokxformalin-fixed paraffin-embedded (FFPE) specimens using the cobas® DNA Sample Preparation Kit (Roche) (cases 1–3) or the Maxwell RSC 48 instrument (Promega) (case 4) using the Maxwell® RSC FFPE Plus DNA Purification Kit (Promega), according to the

(169) African-Lineage Zika Virus Replication Dynamics and Maternal-Fetal Interface Infection in Pregnant Rhesus MacaquesJ VirolJune 2, 2021Chelsea M. Crooks, Andrea M. Weiler, Sierra L. Rybarczyk, Mason Bliss, Anna S. Jaeger, Megan E. Murphy, Heather A. Simmons, Andres Mejia, Michael K. Fritsch, Jennifer M. Hayes, et al.plasma aliquot using the Viral Total Nucleic Acid kit (Promega, Madison, WI) on a Maxwell 16 MDx or Maxwell RSC 48 instrument (Promega, Madison, WI). Viral RNA isolation from tissues. Tissue samples, cut to a 0.5-cm thickness

(170) BED domain‐containing NLR from wild barley confers resistance to leaf rustPlant Biotechnology JournalJune 1, 2021Chunhong Chen, Matthias Jost, Bethany Clark, Matthew Martin, Oadi Matny, Brian J. Steffenson, Jerome D. Franckowiak, Martin Mascher, Davinder Singh, Dragan Perovic, et al.sampled at the 2nd‐3rd leaf stage for RNA extraction. RNA was extracted using Plant RNA Kit (Promega) on Maxwell® RSC 48 Instrument (Promega) as per manufacturer’s instructions. cDNA was synthesized from 1 µg of total RNA using SuperScript™ III Reverse Transcriptase

(171) Comparison of the egg recovery rates and limit of detection for soil-transmitted helminths using the Kato-Katz thick smear, faecal flotation and quantitative real-time PCR in human stoolPLOS Neglected Tropical DiseasesMay 26, 2021Patsy A. Zendejas-Heredia, Vito Colella, Sze Fui Hii, Rebecca J. TraubZirconia/Silica beads (Daintree Scientific, AUS). Following beadbeating, cell lysis was proceeded in a Maxwell RSC 48 Instrument, Promega. The final eluted sample (100 μL) was stored at -20˚C for further downstream analyses. DNA

(172) Rapid Evolution of Enhanced Zika Virus Virulence during Direct Vertebrate Transmission ChainsJournal of VirologyMarch 25, 2021Kasen K. Riemersma, Anna S. Jaeger, Chelsea M. Crooks, Katarina M. Braun, James Weger-Lucarelli, Gregory D. Ebel, Thomas C. Friedrich, Matthew T. AliotaRNA isolation. RNA was isolated with the Maxwell RSC viral total nucleic acid purification kit on a Maxwell RSC 48 instrument (Promega). Isolated ZIKV RNA was titrated by qRT-PCR using TaqMan Fast virus 1-step master mix (ThermoFisher)

(173) A Non-Toxic Concentration of Telomerase Inhibitor BIBR1532 Fails to Reduce TERT Expression in a Feeder-Free Induced Pluripotent Stem Cell Model of Human Motor NeurogenesisInternational Journal of Molecular SciencesMarch 23, 2021Virenkumar A. Pandya, Hamish Crerar, Jamie S. Mitchell, Rickie Patanidirectly from cells using the Maxwell RSC simplyRNA cells kit (Promega AS1390, Madison, WI, USA) and Maxwell RSC 48 Instrument, as per the manufacturer’s instructions. cDNA synthesis was performed using the RevertAid cDNA synthesis

(174) Rapid Evolution of Enhanced Zika Virus Virulence during Direct Vertebrate Transmission ChainsJ VirolFebruary 3, 2021Kasen K. Riemersma, Anna S. Jaeger, Chelsea M. Crooks, Katarina M. Braun, James Weger-Lucarelli, Gregory D. Ebel, Thomas C. Friedrich, Matthew T. AliotaRNA isolation. RNA was isolated with the Maxwell RSC viral total nucleic acid purification kit on a Maxwell RSC 48 instrument (Promega). Isolated ZIKV RNA was titrated by qRT-PCR using TaqMan Fast virus 1-step master mix (ThermoFisher)

(175) The secondary KIT mutation p.Ala510Val in a cutaneous mast cell tumour carrying the activating mutation p.Asn508Ile confers resistance to masitinib in dogsBMC Veterinary ResearchDecember 1, 2020Fabio Gentilini, Maria Elena Turba, Claire Dally, Masamine Takanosu, Sena Kurita, Makoto Bonkobaraspecimens of both cutaneous samples and renal masses using a Maxwell® FFPE Plus DNA Kit run on the Maxwell® RSC 48 Instrument (Promega, Milan, Italy) according to the manufacturer’s instructions. The renal sample was examined

(176) Evaluation of the Ion AmpliSeq™ PhenoTrivium Panel: MPS-Based Assay for Ancestry and Phenotype Predictions Challenged by Casework SamplesGenes (Basel)November 25, 2020Marta Diepenbroek, Birgit Bayer, Kristina Schwender, Roberta Schiller, Jessica Lim, Robert Lagacé, Katja AnslingerPreparation and Sequencing For all samples used in the study, genomic DNA (gDNA) were extracted on the Maxwell® RSC 48 Instrument using the Maxwell® FSC DNA IQ™ Casework Kit as recommended by the manufacturer (Promega). The extracts

(177) Revealing fine-scale spatiotemporal differences in SARS-CoV-2 introduction and spreadNature CommunicationsNovember 3, 2020Gage K. Moreno, Katarina M. Braun, Kasen K. Riemersma, Michael A. Martin, Peter J. Halfmann, Chelsea M. Crooks, Trent Prall, David Baker, John J. Baczenas, Anna S. Heffron, et al.100 μL of VTM using the Viral Total Nucleic Acid Purification kit (Promega, Madison, WI, USA) on a Maxwell RSC 48 instrument and was eluted in 50 μL of nuclease free H2O. Complementary DNA (cDNA) generation Complementary DNA

(178) Microbiota modulate sympathetic neurons via a gut-brain circuitNatureJuly 1, 2020Muller PA, Schneeberger M, Matheis F, Wang P, Kerner Z, Ilanges A, Pellegrino K, Del Mármol J, Castro TBR, Furuichi M, et al.to calculate significance. 16S sample processing. 16s samples were processed utilizing a Promega Maxwell® RSC 48 Instrument. Following DNA extraction from all samples, DNA samples were quantified using a ThermoFisher Quant-It

(179) Bacterial metabolism of bile acids promotes generation of peripheral regulatory T cellsNatureMay 1, 2020Campbell C, McKenney PT, Konstantinovsky D, Isaeva OI, Schizas M, Verter J, Mai C, Jin WB, Guo CJ, Violante S, et al.transferred to a MaxPrep Liquid Handler tube rack (Promega) and the Maxwell® RSC48 instrument (Promega, AS8500) was loaded with proteinase K tubes, lysis buffer, elution buffer, pipetting tips, 96-sample deep-well

(180) ATP2A2 SINE Insertion in an Irish Terrier with Darier Disease and Associated Infundibular Cyst FormationGenes (Basel)April 28, 2020Monika Linek, Maren Doelle, Tosso Leeb, Anina Bauer, Fabienne Leuthard, Jan Henkel, Danika Bannasch, Vidhya Jagannathan, Monika M. WelleWhole Genome Sequencing Genomic DNA was isolated from EDTA blood of the affected dog with the Maxwell RSC Whole Blood Kit using a Maxwell RSC 48 instrument (Promega, Madison, WI, USA). An Illumina TruSeq PCR-free DNA library (Illumina, San Diego, CA, USA)

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