Maxwell® RSC 48 Instrument from Promega

Product Specs
ItemMaxwell® RSC 48 Instrument
CompanyPromega
Price Supplier Page
Catalog NumberAS8500
QuantityEA

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(161) Technical Performance of a 430-Gene Preventative Genomics Assay to Identify Multiple Variant Types Associated with Adult-Onset Monogenic Conditions, Susceptibility Loci, and Pharmacogenetic InsightsJournal of Personalized MedicineApril 21, 2022Ari Silver, Gabriel A. Lazarin, Maxwell Silver, Meghan Miller, Michael Jansen, Christine Wechsberg, Erin Dekanek, Stav Grossfeld, Tim Herpel, Dinura Gunatilake, et al.blood specimens using the Maxwell® RSC Blood DNA Kit (Promega, Madison, Wisconsin) on the automated Maxwell® RSC 48 Instrument (Promega), as well as being sourced from the Coriell Cell Repository. The validation set included 157

(162) DNA Methylation Regulates a Set of Long Non-Coding RNAs Compromising Hepatic Identity during HepatocarcinogenesisCancers (Basel)April 19, 2022Miriam Recalde et al.frozen tissues and cultured cells was isolated using the Maxwell® RSC Cultured Cells DNA Purification Kit with a Maxwell® RSC 48 instrument (Promega, Madison, WI, USA; AS1620). DNA purity and concentration were measured using a NanoDrop spectrophotometer

(163) Molecular Detection of Equine Adenovirus 1 in Nasal Swabs from Horses in the Republic of KoreaVeterinary SciencesApril 13, 2022Sang-Kyu Lee, Jeechan Choi, Jungho Yoon, Jaemin Jung, Joon-Young Park, Jongyoung Park, Yeonjong Kim, Ji-Young Park, Dongsun Parksamples using a Maxwell® RSC Viral Total Nucleic Acid Purification Kit (Promega, Madison, WI, USA) on a Maxwell® RSC 48 Instrument (Promega) according to the manufacturer’s instructions and stored at −80 °C. For EAdV-1 DNA screening,

(164) Soluble CD137 as a dynamic biomarker to monitor agonist CD137 immunotherapiesJournal for ImmunoTherapy of CancerMarch 2, 2022Javier Glez-Vaz, Arantza Azpilikueta, Irene Olivera, Assunta Cirella, Alvaro Teijeira, Maria C Ochoa, Maite Alvarez, IÃ±aki Eguren-Santamaria, Carlos Luri-Rey, Maria E Rodriguez-Ruiz, et al.RT-qPCR Total RNA was extracted from CD8 and PBMCs using a Maxwell RSC simplyRNA tissue kit (Promega) in a Maxwell RSC 48 instrument. M-MLV reverse transcriptase (Invitrogen) and random primers (Invitrogen) were used to obtain cDNA from

(165) SARS-CoV-2 N Gene G29195T Point Mutation May Affect Diagnostic Reverse Transcription-PCR DetectionMicrobiology SpectrumFebruary 23, 2022Karrie K. K. Ko, Nurdyana Binte Abdul Rahman, Shireen Yan Ling Tan, Kenneth X. L. Chan, Sui Sin Goh, James Heng Chiak Sim, Kun Lee Lim, Wan Loo Tan, Kian Sing Chan, Lynette L. E. Oon, et al.laboratory. Total nucleic acid extraction of the chemically inactivated remnant samples was performed on a Maxwell RSC 48 instrument, using the Maxwell RSC viral total nucleic acid purification kit (Promega, USA). The resultant total

(166) A novel Betaretrovirus discovered in cattle with neurological disease and encephalitisRetrovirologyDecember 20, 2021Melanie M. Hierweger, Michel C. Koch, Ronja V. Kauer, Zoltán Bagó, Anna Oevermann, Giuseppe Bertoni, Torsten Seuberlichslaughtered control animals, fresh-frozen medulla oblongata was used. Extraction was performed on the Maxwell® RSC 48 instrument (Promega Corporation, Madison, WI, USA) using the Maxwell® RSC PureFood GMO and Authentication Kit for

(167) Single-cell RNA sequencing reveals distinct tumor microenvironmental patterns in lung adenocarcinomaOncogeneDecember 16, 2021Philip Bischoff, Alexandra Trinks, Benedikt Obermayer, Jan Patrick Pett, Jennifer Wiederspahn, Florian Uhlitz, Xizi Liang, Annika Lehmann, Philipp Jurmeister, Aron Elsner, et al.sections. DNA was isolated using the Maxwell RSC DNA FFPE Kit (Promega, Madison, Wisconsin, USA) on a Maxwell RSC 48 Instrument (Promega) and analyzed using the nNGM panel v1 (ThermoFisher, Waltham, Massachusetts, USA), an Ion 530

(168) Population genomics and haplotype analysis in spelt and bread wheat identifies a gene regulating glume colorCommunications BiologyDecember 1, 2021Michael Abrouk et al.were collected for RNA isolation. RNA extraction was done using the Maxwell® RSC Plant RNA kit using a Maxwell® RSC 48 instrument (Promega). In all, 1 µg of RNA was used for the first-strand complementary DNA (cDNA) synthesis using

(169) Molecular Prevalence of Equine Parvovirus-Hepatitis in the Sera of Clinically Healthy Horses in South KoreaVeterinary SciencesNovember 19, 2021Sang-Kyu Lee, Dongsun Park, Inhyung Leeusing the Maxwell® RSC Viral Total Nucleic Acid Purification Kit (Promega, Madison, WI, USA) on the Maxwell® RSC 48 Instrument (Promega) according to the manufacturer’s instructions and stored at –70 °C until use. For EqPV-H DNA

(170) Enhanced rapid commercial DNA extraction kit for the molecular detection of severe acute respiratory syndrome coronavirus 2: Easy adaptation to current protocolsRevista da Sociedade Brasileira de Medicina TropicalNovember 12, 2021Rômulo Pessoa-e-Silva, Priscilla Stela Santana de de Oliveira, Sayonara Maria Calado Gonçalves, Klarissa Miranda Guarines, Lidiane Vasconcelos do Nascimento Carvalho, Maria Andreza Bezerra Correia, Michelle Melgarejo da da Rosa, Moacyr Jesus Barreto de Melo Rêgo, Maira Galdino da Rocha Pitta, Michelly Cristiny Pereirawere the Maxwell® RSC Viral Total Nucleic Acid kit (AS1330), for use in robotic extraction with the Maxwell RSC 48 Instrument (Promega, Madison, WI, USA) using magnetic beads (sample volume, 200 µL; elution volume, 50 µL of RNase-free

(171) SARS-CoV-2 Infection in One Cat and Three Dogs Living in COVID-19-Positive Households in Madrid, SpainFrontiers in Veterinary ScienceNovember 10, 2021Guadalupe Miró, Javier Regidor-Cerrillo, Rocio Checa, Carlos Diezma-Díaz, Ana Montoya, Jesús García-Cantalejo, Pedro Botías, Javier Arroyo, Luis-Miguel Ortega-Moramedium using the Maxwell® RSC Viral Total Nucleic Acid Purification Kit for automated extraction in a Maxwell® RSC 48 Instrument (Promega, Madrid, Spain). Viral RNA detection was performed using the multiplex TaqPath™ COVID-19 CE-IVD

(172) Tongue nodules in an atypical canine leishmaniasis in BrazilJournal of Veterinary Medical ScienceOctober 5, 2021João José da COSTA NETO, Camila Neves MARTINS, Karen Santos MARÇO, Beatriz Furlan PAZ, Guilherme Paz MONTEIRO, Roberta Torres de de MELO, Francisco Cláudio Dantas MOTA, Aracelle Elisane ALVESqPCR was performed to confirm the molecular identity of the Leishmania spp. The DNA was extracted (Maxwell® RSC 48 Instrument) (Promega, São Paulo, Brazil) from a sample collected from the animal’s bone marrow and tested for conserved

(173) Viral Sequencing to Investigate Sources of SARS-CoV-2 Infection in US Healthcare PersonnelClinical Infectious DiseasesSeptember 15, 2021Katarina M Braun, Gage K Moreno, Ashley Buys, Elizabeth D Somsen, Max Bobholz, Molly A Accola, Laura Anderson, William M Rehrauer, David A Baker, Nasia Safdar, et al.Briefly, viral RNA was extracted using the Viral Total Nucleic Acid Purification kit (Promega) on a Maxwell RSC 48 instrument. Complementary DNA was synthesized using SuperScript IV reverse-transcriptase [24, 25]. SARS-CoV-2–specific

(174) Pathogenicity of H9N2 low pathogenic avian influenza viruses of different lineages isolated from live bird markets tested in three animal models: SPF chickens, Korean native chickens, and ducksPoultry ScienceSeptember 1, 2021Soo-Jeong Kye, Min-Ji Park, Na-Young Kim, Yu-Na Lee, Gyeong-Beom Heo, Yoon-Ki Baek, Jae-In Shin, Myoung-Heon Lee, Youn-Jeong Leesuspension (supernatant) was subjected to RNA extraction using the Maxwell RSC simplyRNA Tissue kit and a Maxwell RSC 48 instrument (Promega, Germany). The cycle threshold (Ct) value was used to calculate the viral load after real-time

(175) Acute SARS-CoV-2 infections harbor limited within-host diversity and transmit via tight transmission bottlenecksPLOS PathogensAugust 23, 2021Katarina M. Braun, Gage K. Moreno, Cassia Wagner, Molly A. Accola, William M. Rehrauer, David A. Baker, Katia Koelle, David H. O’Connor, Trevor Bedford, Thomas C. Friedrich, et al.100 μl of VTM using the Viral Total Nucleic Acid Purification kit (Promega, Madison, WI, USA) on a Maxwell RSC 48 instrument and eluted in 50 μL of nuclease-free H2O. Complementary DNA (cDNA) generation and PCR Complementary

(176) Zoonotic Soil-Transmitted Helminths in Free-Roaming Dogs, KiribatiEmerging Infectious DiseasesAugust 1, 2021Patsy A. Zendejas-Heredia, Allison Crawley, Helen Byrnes, Rebecca J. Traub, Vito Colellahttps://www.mpbio.com). After bead-beating and cell lysis, we proceeded with DNA purification in a Maxwell RSC 48 Instrument (Promega). We stored the final eluted sample (100 μL) at −20°C for further analyses. We subjected the

(177) Previous exposure to dengue virus is associated with increased Zika virus burden at the maternal-fetal interface in rhesus macaquesPLOS Neglected Tropical DiseasesJuly 30, 2021Chelsea M. Crooks et al.plasma aliquot using the Viral Total Nucleic Acid Kit (Promega, Madison, WI) on a Maxwell 16 MDx or Maxwell RSC 48 instrument (Promega, Madison, WI). Viral RNA isolation from tissues Tissue samples, cut to 0.5 cm thickness on

(178) African-Lineage Zika Virus Replication Dynamics and Maternal-Fetal Interface Infection in Pregnant Rhesus MacaquesJournal of VirologyJuly 26, 2021Chelsea M. Crooks et al.plasma aliquot using the Viral Total Nucleic Acid kit (Promega, Madison, WI) on a Maxwell 16 MDx or Maxwell RSC 48 instrument (Promega, Madison, WI). Viral RNA isolation from tissues. Tissue samples, cut to a 0.5-cm thickness

(179) Severe Acute Respiratory Syndrome Coronavirus 2 Transmission in Intercollegiate Athletics Not Fully Mitigated With Daily Antigen TestingClinical Infectious DiseasesJuly 15, 2021Gage K Moreno, Katarina M Braun, Ian W Pray, Hannah E Segaloff, Ailam Lim, Keith Poulsen, Jonathan Meiman, James Borcher, Ryan P Westergaard, Michael K Moll, et al.100 μL of viral transport medium using the Viral Total Nucleic Acid Purification kit (Promega) on a Maxwell RSC 48 instrument, according to manufacturer guidelines, and was eluted in 50 μL of nuclease-free water. Library Preparation

(180) Expanding spectrum of “spitzoid” lesions: a small series of 4 cases with MAP2K1 mutationsVirchows ArchivJuly 1, 2021K. G. P. Kerckhoffs, T. Aallali, C. A. Ambarus, V. Sigurdsson, A. M. L. Jansen, W. A. M. Blokxformalin-fixed paraffin-embedded (FFPE) specimens using the cobas® DNA Sample Preparation Kit (Roche) (cases 1–3) or the Maxwell RSC 48 instrument (Promega) (case 4) using the Maxwell® RSC FFPE Plus DNA Purification Kit (Promega), according to the

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