Maxwell® RSC 48 Instrument from Promega

Product Specs
ItemMaxwell® RSC 48 Instrument
CompanyPromega
Price Supplier Page
Catalog NumberAS8500
QuantityEA

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(121) Latin American Trans-ancestry INitiative for OCD genomics (LATINO): Study ProtocolmedRxivMarch 1, 2023James J Crowley, Carolina Cappi, Marcos E Ochoa-Panaifo, Renee M Frederick, Minjee Kook, Andrew D Wiese, Diana Rancourt, Elizabeth G Atkinson, Paola Giusti-Rodriguez, Jacey L Anderberg, et al.of saliva is extracted in an automated fashion using Maxwell® RSC Stabilized Saliva DNA Kits and a Maxwell® RSC 48 Instrument from Promega (Madison, Wisconsin, USA). The remaining saliva solution is frozen at −80 degrees C. 2.1.5

(122) Wastewater surveillance of SARS-CoV-2 and influenza in preK-12 schools shows school, community, and citywide infectionsWater ResearchMarch 1, 2023Madeline Wolken, Thomas Sun, Camille McCall, Rebecca Schneider, Kelsey Caton, Courtney Hundley, Loren Hopkins, Katherine Ensor, Kaavya Domakonda, Prashant Kalvapalle, et al.2020 and January 25, 2021, RNA extraction was performed using the Maxwell 48 RSC automated platform (AS8500, Promega) and the modified protocol for the Maxwell RSC PureFood GMO and Authentication Kit (AS1600,

(123) A parasitic fungus employs mutated eIF4A to survive on rocaglate-synthesizing Aglaia plantsElifeFebruary 28, 2023Mingming Chen, Naoyoshi Kumakura, Hironori Saito, Ryan Muller, Madoka Nishimoto, Mari Mito, Pamela Gan, Nicholas T Ingolia, Ken Shirasu, Takuhiro Ito, et al.beads Other,Shakemaster NEO,BMS,Cat. #:BMS-mini16,Bead mill homogenizer Other,Maxwell RSC 48 Instrument,Promega,Cat. #:AS8500,Automated nucleic acid purification platform Other,MX3000P Real-Time qPCR System,Stratagene,Cat. #:401511,Real-time

(124) A parasitic fungus employs mutated eIF4A to survive on rocaglate-synthesizing Aglaia plantseLifeFebruary 28, 2023Mingming Chen, Naoyoshi Kumakura, Hironori Saito, Ryan Muller, Madoka Nishimoto, Mari Mito, Pamela Gan, Nicholas T Ingolia, Ken Shirasu, Takuhiro Ito, et al.beads Other,Shakemaster NEO,BMS,Cat. #:BMS-mini16,Bead mill homogenizer Other,Maxwell RSC 48 Instrument,Promega,Cat. #:AS8500,Automated nucleic acid purification platform Other,MX3000P Real-Time qPCR System,Stratagene,Cat. #:401511,Real-time

(125) Implementation of Exome Sequencing in Prenatal Diagnostics: Chances and ChallengesDiagnostics (Basel)February 23, 2023Ewa Janicki, Marjan De Rademaeker, Colombine Meunier, Nele Boeckx, Bettina Blaumeiser, Katrien Janssenseither amniotic fluid, chorion villi or cultured amniocytes using the Maxwell RSC Blood DNA kit on a Maxwell RSC 48 Instrument (Promega, Madison, MI, USA). Library prep on 50ng of genomic DNA was performed using the Twist Human Core Exome kit

(126) SARS-CoV-2 transmission in intercollegiate athletics not fully mitigated with daily antigen testingmedRxivFebruary 6, 2023Gage K. Moreno, Katarina M. Braun, Ian W. Pray, Hannah E. Segaloff, Ailam Lim, Keith Poulson, Jonathan Meiman, James Borcher, Ryan P. Westergaard, Michael K. Moll, et al.100 μl of VTM using the Viral Total Nucleic Acid Purification kit (Promega, Madison, WI, USA) on a Maxwell RSC 48 instrument, using manufacturer guidelines, and was eluted in 50 μL of nuclease free H2O. Complementary DNA (cDNA)

(127) Case Report: Disease progression of renal cell carcinoma containing a novel putative pathogenic KAT6A::NRG1 fusion on Ipilimumab- Nivolumab immunotherapy. A case study and review of the literatureFrontiers in OncologyFebruary 2, 2023Almas Dawood, Suzanne MacMahon, My-Anh Tran Dang, Maxine G. B. Tran, Axel Bex, Ekaterini Boleti, Soha El Sheikhwas isolated from formalin-fixed paraffin embedded (FFPE) tissue sections, using RSC RNA FFPE Kit and Maxwell® RSC 48 Instrument (Promega, WI, USA). Quantifying the RNA concentration with the Qubit 3 Fluorometer (Thermo Fisher Scientific,

(128) An environmentally relevant concentration of antibiotics impairs the immune system of zebrafish (Danio rerio) and increases susceptibility to virus infectionFrontiers in ImmunologyJanuary 12, 2023Patricia Pereiro, Magalí Rey-Campos, Antonio Figueras, Beatriz Novoaor antibiotics was extracted using the Maxwell RSC simplyRNA Tissue kit (Promega) with an automated Maxwell® RSC 48 Instrument in accordance with the manufacturer’s instructions. For the samples obtained from the sublethal infection,

(129) Mucociliary transport deficiency and disease progression in Syrian hamsters with SARS-CoV-2 infectionJCI InsightJanuary 10, 2023Qian Li, Kadambari Vijaykumar, Scott E. Phillips, Shah S. Hussain, Nha V. Huynh, Courtney M. Fernandez-Petty, Jacelyn E. Peabody Lever, Jeremy B. Foote, Janna Ren, Javier Campos-Gómez, et al.temperature for 10 minutes and at 56°C for an additional 10 minutes, RNA was extracted utilizing the Maxwell RSC 48 Instrument (Promega Corporation), and qRT2-PCR was performed utilizing a QuantStudio 5 Real-Time PCR instrument

(130) Citywide wastewater SARS-CoV-2 levels strongly correlated with multiple disease surveillance indicators and outcomes over three COVID-19 wavesScience of The Total EnvironmentJanuary 10, 2023Loren Hopkins, David Persse, Kelsey Caton, Katherine Ensor, Rebecca Schneider, Camille McCall, Lauren B. Stadlerand January 25, 2021, nucleic acid extraction was performed on a Maxwell 48 RSC automated platform (AS8500, Promega) using a modified protocol for the Maxwell RSC PureFood GMO and Authentication Kit (AS1600,

(131) Monitoring monkeypox virus in saliva and air samples in Spain: a cross-sectional studyThe Lancet MicrobeJanuary 1, 2023Bruno Hernaez, Ana Muñoz-Gómez, Africa Sanchiz, Eva Orviz, Adrian Valls-Carbo, Iñigo Sagastagoitia, Oskar Ayerdi, Rocío Martín, Teresa Puerta, Mar Vera, et al.cell lysis buffer, we used the Viral Total Nucleic Acid Purification kit (Promega) in combination with a Maxwell RSC 48 Instrument (Promega). To enhance the recovery of DNA, 100 ng of human DNA (from HeLa cells) were added to each

(132) Microbiota manipulation to increase macrophage IL-10 improves colitis and limits colitis-associated colorectal cancerGut MicrobesDecember 31, 2022Daniel F. Zegarra Ruiz, Dasom V. Kim, Kendra Norwood, Fatima B. Saldana-Morales, Myunghoo Kim, Charles Ng, Ryann Callaghan, Maisha Uddin, Lin-Chun Chang, Randy S. Longman, et al.were collected sterilely and stored at −80 C. DNA was extracted utilizing Promega Max Prep & Promega Maxwell RSC 48 Instrument. The V4 and V5 regions of the 16S rDNA were PCR amplified, normalized, pooled, and sequenced using the

(133) Strigol induces germination of the facultative parasitic plant Phtheirospermum japonicum in the absence of nitrate ionsPlant Signaling & BehaviorDecember 31, 2022Satoshi Ogawa, Ken ShirasuMaxwell RSC Plant RNA Kit (Promega, Cat. No. AS1500) and a Maxwell RSC 48 instrument (Promega, Cat. No. AS8500). Reverse transcription and RT-qPCR were performed as previously described.23 Polyubiquitin C 2 (UBC2)

(134) Fast Air-to-Liquid Sampler Detects Surges in SARS-CoV-2 Aerosol Levels in Hospital RoomsInternational Journal of Environmental Research and Public HealthDecember 29, 2022Cristina del Álamo, Ángela Vázquez-Calvo, África Sanchiz, Gil Rodríguez-Caravaca, Rocío Martín, Bruno Hernáez, Pablo Méndez-Vigo-Carranza, Juan Sánchez García-Casarrubios, Antonio Alcamí, José Luis Pérez-Díaz600 µL, after adding 30 µL of proteinase K, following Maxwell® RSC Viral TNA kit instructions, in a Maxwell® RSC 48 Instrument (Promega) isolation. RNA was eluted in 50 µL of nuclease free water. In every RNA extraction, a blank

(135) Distinct patterns of SARS-CoV-2 transmission in two nearby communities in Wisconsin, USAmedRxivDecember 6, 2022Gage Kahl Moreno, Katarina M. Braun, Kasen K. Riemersma, Michael A. Martin, Peter J. Halfmann, Chelsea M Crooks, Trent Prall, David Baker, John J. Baczenas, Anna S. Heffron, et al.100 μl of VTM using the Viral Total Nucleic Acid Purification kit (Promega, Madison, WI, USA) on a Maxwell RSC 48 instrument and was eluted in 50 μL of nuclease free H2O. vRNA isolation for index Dane County Sample Approximately

(136) RT-qPCR-based tests for SARS-CoV-2 detection in pooled saliva samples for massive population screening to monitor epidemicsScientific ReportsDecember 1, 2022Michał Różański et al.instructions of isolation RNA using the Maxwell RSC Viral Total Nucleic Acid Purification Kit with the Maxwell RSC 48 Instrument (Promega, Madison, WI, USA). Briefly, 300 μl saliva was mixed with 330 µl lysis buffer containing proteinase

(137) A single intranasal administration of AdCOVID protects against SARS-CoV-2 infection in the upper and lower respiratory tractsHuman Vaccines & ImmunotherapeuticsNovember 30, 2022Michael D. Schultz, John J. Suschak, Davide Botta, Aaron Silva-Sanchez, R. Glenn King, Thomas W. Detchemendy, Chetan D. Meshram, Jeremy B. Foote, Fen Zhou, Jennifer L. Tipper, et al.#AS1330). The samples were mixed and incubated at 56ºC for 10 minutes. RNA was extracted utilizing the Maxwell RSC 48 Instrument (Promega, Madison, WI) and qRT-PCR performed utilizing QuantStudio™ 5 Real-Time PCR instrument (ThermoFisher,

(138) Monitoring monkeypox virus in saliva and air samples in Spain: a cross-sectional studyLancet MicrobeNovember 24, 2022Bruno Hernaez, Ana Muñoz-Gómez, Africa Sanchiz, Eva Orviz, Adrian Valls-Carbo, Iñigo Sagastagoitia, Oskar Ayerdi, Rocío Martín, Teresa Puerta, Mar Vera, et al.cell lysis buffer, we used the Viral Total Nucleic Acid Purification kit (Promega) in combination with a Maxwell RSC 48 Instrument (Promega). To enhance the recovery of DNA, 100 ng of human DNA (from HeLa cells) were added to each

(139) Protein aggregation and calcium dysregulation are hallmarks of familial Parkinson’s disease in midbrain dopaminergic neuronsnpj Parkinson's DiseaseNovember 24, 2022Gurvir S. Virdi, Minee L. Choi, James R. Evans, Zhi Yao, Dilan Athauda, Stephanie Strohbuecker, Raja S. Nirujogi, Anna I. Wernick, Noelia Pelegrina-Hidalgo, Craig Leighton, et al.snap-frozen cell pellets using the Maxwell® RSC simply RNA Cells kit (Promega), and the accompanying Maxwell® RSC 48 instrument. After RNA extraction, the RNA concentration and quality using the 260/280 ratio were assessed using

(140) Genetic Characterization of African Swine Fever Virus from Pig Farms in South Korea during Outbreaks in 2019–2021VirusesNovember 24, 2022Ki-Hyun Cho, Da-Young Kim, Min-Kyung Jang, Seong-Keun Hong, Ji-Hyoung Ryu, Hae-Eun Kang, Jee-Yong ParkMaxwell® RSC viral total nucleic acid purification kit (Promega, Madison, Wisconsin, USA) for tissue homogenates, processed through the Maxwell® RSC 48 instrument, according to the manufacturer’s instructions. To genetically characterize the ASFV isolates, 12 gene

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