Maxwell® RSC 48 Instrument from Promega

Product Specs
ItemMaxwell® RSC 48 Instrument
CompanyPromega
Price Supplier Page
Catalog NumberAS8500
QuantityEA

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(81) Unravelling turbot (Scophthalmus maximus) resistance to Aeromonas salmonicida: transcriptomic insights from two full-sibling families with divergent susceptibilityFrontiers in ImmunologyDecember 6, 2024Patricia Pereiro, Ricardo Tur, Miguel García, Antonio Figueras, Beatriz Novoavalidation RNA was isolated with the Maxwell RSC simplyRNA Tissue Kit (Promega) with an automated Maxwell RSC 48 Instrument following the guidelines outlined by the manufacturer. The quantity of RNA was measured with a NanoDrop

(82) Oxidative Stress and Inflammation-Related mRNAs Are Elevated in Serum of a Finnish Wet AMD CohortInvestigative Ophthalmology & Visual ScienceNovember 15, 2024Mikko Liukkonen, Hanna Heloterä, Leea Siintamo, Bishwa Ghimire, Pirkko Mattila, Niko Kivinen, Joanna Kostanek, Cezary Watala, Maria Hytti, Juha Hyttinen, et al.of Life Science and Biocenter Finland. Total RNA was extracted from the serum samples (1 mL) using Maxwell RSC 48 Instrument and Maxwell RSC miRNA Plasma and Serum Kit (Promega, Madison, WI) according to the manufacturer's instructions,

(83) Effect of nicotinamide riboside on airway inflammation in COPD: a randomized, placebo-controlled trialNature AgingNovember 15, 2024Kristoffer L. Norheim, Michael Ben Ezra, Indra Heckenbach, Louise Munkholm Andreasson, Lise Lotte Eriksen, Nanna Dyhre-Petersen, Mads Vargas Damgaard, Magnus Berglind, Luca Pricolo, Dayle Sampson, et al.AutoAnnotate. DNA methylation PBMCs were shipped on dry ice and analyzed by Tempus Labs. DNA was isolated on a Maxwell RSC 48 instrument using the Maxwell RSC Blood DNA Kit (Promega, AS1400) according to the manufacturer’s protocol. In brief,

(84) Comprehensive in silico CpG methylation analysis in hepatocellular carcinoma identifies tissue- and tumor-type specific marks disconnected from gene expressionJournal of Physiology and BiochemistryNovember 1, 2024Idoia Bilbao, Miriam Recalde, Fabrice Daian, José Maria Herranz, María Elizalde, Mercedes Iñarrairaegui, Matteo Canale, Maite G. Fernández-Barrena, Andrea Casadei-Gardini, Bruno Sangro, et al.from frozen tissues was isolated using the Maxwell® RSC Cultured Cells DNA Purification Kit with a Maxwell® RSC 48 instrument (Promega, Madison, WI, USA; AS1620). DNA purity and concentration were measured using a NanoDrop spectrophotometer

(85) Rethinking asexuality: the enigmatic case of functional sexual genes in Lepraria (Stereocaulaceae)BMC GenomicsOctober 26, 2024Meredith M. Doellman, Yukun Sun, Alejandrina Barcenas-Peña, H. Thorsten Lumbsch, Felix Grewethe Qiagen TissueLyser II (Qiagen, Hilden, Germany). The extraction was completed according to the Maxwell® RSC 48 Instrument (Promega, Madison, WI) protocol. Stranded poly(a) selected libraries were then prepared with the NEXTFLEX®

(86) Impaired neutrophil-mediated cell death drives Ewing’s Sarcoma in the background of Down syndromeFrontiers in OncologyOctober 3, 2024Serena Peirone, Elisa Tirtei, Anna Campello, Caterina Parlato, Simonetta Guarrera, Katia Mareschi, Elena Marini, Sebastian Dorin Asaftei, Luca Bertero, Mauro Papotti, et al.μm-thick FFPE sections (3–6 sections per sample) using Maxwell® RSC DNA FFPE Kit (Promega Corporation) on Maxwell® RSC 48 Instrument (Promega Corporation), following the manufacturer’s protocol. Peripheral blood was used as a matching

(87) A preliminary indication that HLA-A*03:01 may be associated with visceral leishmaniasis development in people living with HIV in EthiopiaPLOS Neglected Tropical DiseasesSeptember 30, 2024Nicky de Vrij, Romi Vandoren, Kadrie Ramadan, Anke Van Hul, Ann Ceulemans, Mekibib Kassa, Roma Melkamu, Arega Yeshanew, Tadfe Bogale, Hailemariam Beyene, et al.extraction and quantification DNA was extracted from the PBMC samples of all participants using the Maxwell RSC 48 instrument (Promega, Wisconsin, U.S.A) using the Maxwell RSC Whole Blood DNA kit (Promega, Wisconsin, U.S.A.) according

(88) Comparison of commercial DNA kits for allergen detection of celery in food matricesHeliyonSeptember 15, 2024Marleen M. Voorhuijzen-Harink, Bas J. Fronen, Linda Willemsen, Andries Koops, Elise F. Hoek-van den Hil, Nathalie G.E. Smitsof the supernatant was transferred into a Maxwell cartridge, and samples were further purified in a Maxwell® RSC 48 instrument (Promega, Wisconsin, USA) using the PureFood GMO and authentication method. DNA was eluted in 100 μL

(89) A comparative field efficacy trial of three treatment programs against endo- and ectoparasites in naturally infected dogsFrontiers in Veterinary ScienceSeptember 5, 2024Cameron Raw, Rebecca J. Traub, Anke Wiethoelter(Catalog no. AS1600, Promega Corporation, Madison, USA) with the Maxwell® RSC 48 Instrument (Catalog no. AS8500, Promega Corporation, Madison, USA) using a modified method as described by Massetti et al. (26). Extracted

(90) Selection for high and low antibody responses to sheep red blood cells influences cytokine and chemokine expression in chicken peripheral blood leukocytes and splenic tissuePoultry ScienceSeptember 1, 2024Christina L. Swaggerty, Paul B. Siegel, Christa F. Honaker, Michael H. Kogut, Robin C. Anderson, Christopher M. Ashwell, Robert L. Taylorthen loaded into the Maxwell cartridge for RNA isolation. All RNA extractions were carried out on the Maxwell® RSC 48 instrument (Promega Corp.). Upon completion, the purified RNA samples were stored at -80°C. Quantitative Real-Time RT-PCR

(91) Lipid-encapsulated mRNA encoding an extended serum half-life interleukin-22 ameliorates metabolic disease in miceMolecular metabolismAugust 1, 2024Canali S, Fischer AW, Nguyen M, Anderson K, Wu L, Graham AR, Hsiao CJ, Bankar C, Dussault N, Ritchie V, et al.Maxwell RSC simplyRNA Tissue Kit (Promega, cat no. AS1340) and automated Maxwell RSC 48 instrument (Promega, cat no. AS8500). Total liver RNA was isolated using QIAshredder columns (Qiagen, cat no. 79656) and RNeasy mini purification

(92) A GWAS study highlights significant associations between a series of indels in a FLOWERING LOCUS T gene promoter and flowering time in white lupin (Lupinus albus L.)BMC Plant BiologyJuly 29, 2024Sandra Rychel-Bielska, Wojciech Bielski, Anna Surma, Paolo Annicchiarico, Jolanta Belter, Bartosz Kozak, Renata Galek, Nathalie Harzic, Michał KsiążkiewiczMaxwell® RSC PureFood GMO and Authentication Kit (Promega, Mannheim, Germany) harnessing automated station Maxwell® RSC 48 Instrument (Promega) with standard protocol, yielding on average 100 ± 29 µg DNA per sample. DNA concentration

(93) The Influence of β-Carotene and Its Liposomal Form on the Expression of EMT Markers and Androgen-Dependent Pathways in Different Prostate Cell LinesAntioxidants (Basel)July 25, 2024Joanna Dulińska-Litewka, Kacper Dykas, Stanisław Boznański, Przemysław Hałubiec, Marta Kaczor-Kamińska, Jacek Zagajewski, Torsten Bohn, Gracjan Wątor(Promega, Madison, WI, USA). The procedure applied paramagnetic particles (as a mobile solid phase) and the Maxwell RSC 48 Instrument (Promega, Madison, WI, USA). The concentration and purity of RNA were determined with the use of Nanodrop

(94) A multicenter genomic epidemiological investigation in Brazil, Chile, and Mexico reveals the diversity and persistence of Salmonella populations in surface watersmBioJuly 17, 2024Zhao Chen, Andrea I. Moreno-Switt, Angelica Reyes-Jara, Enrique Delgado Suarez, Aiko D. Adell, Celso José Bruno Oliveira, Raquel Regina Bonelli, Xinyang Huang, Eric Brown, Marc Allard, et al.individual colonies were grown overnight in TSB broth (BD, Sparks, MD, USA) at 37°C. DNA was purified on a Maxwell RSC-48 Instrument using the Cultured Cells DNA Kit (Promega, Madison, WI, USA). Libraries were prepared with the Illumina

(95) Cerebrospinal Fluid cfDNA Sequencing for Classification of Central Nervous System GliomaClinical Cancer ResearchJuly 15, 2024Florian Iser, Felix Hinz, Dirk C. Hoffmann, Niklas Grassl, Cansu Güngoör, Jochen Meyer, Laura Dörner, Lea Hofmann, Vanessa Kelbch, Kirsten Göbel, et al.formalin-fixed paraffin-embedded (FFPE) tissue using the Maxwell RSC Buffy Coat DNA Kit and Maxwell RSC DNA FFPE Kit on a Maxwell RSC 48 instrument (Promega) according to the manufacturer’s recommendations. Preparation of NGS libraries For gDNA derived

(96) SGLT2 inhibition eliminates senescent cells and alleviates pathological agingNat AgingJuly 1, 2024Katsuumi G, Shimizu I, Suda M, Yoshida Y, Furihata T, Joki Y, Hsiao CL, Jiaqi L, Fujiki S, Abe M, et al.and QUantiStudio6 Pro. RNA sequencing analysis Total RNA was extracted from mouse gWAT using the Maxwell RSC 48 Instrument (Promega) and Maxwell RSC simplyRNA Tissue Kit (Promega, cat. no. AS1340) in accordance with the manufacturer’s

(97) Detection of hidden antibiotic resistance through real-time genomicsNature CommunicationsJune 28, 2024Ela Sauerborn, Nancy Carolina Corredor, Tim Reska, Albert Perlas, Samir Vargas da Fonseca Atum, Nick Goldman, Nina Wantia, Clarissa Prazeres da Costa, Ebenezer Foster-Nyarko, Lara Urbanthrough the Maxwell® RSC Blood DNA extraction protocol for Gram-negative bacteria with the Promega Maxwell®RSC 48 Instrument (Promega Corporation, Madison, USA). DNA concentrations were measured using the QubitTM (Thermo Fischer

(98) Latin American Trans‐ancestry INitiative for OCD genomics (LATINO): Study protocolAmerican Journal of Medical Genetics Part B: Neuropsychiatric GeneticsJune 1, 2024James J. Crowley, Carolina Cappi, Marcos E. Ochoa‐Panaifo, Renee M. Frederick, Minjee Kook, Andrew D. Wiese, Diana Rancourt, Elizabeth G. Atkinson, Paola Giusti‐Rodriguez, Jacey L. Anderberg, et al.of saliva is extracted in an automated fashion using Maxwell® RSC Stabilized Saliva DNA Kits and a Maxwell® RSC 48 Instrument from Promega (Madison, Wisconsin, USA). The remaining saliva solution is frozen at −80 degrees C. 2.1.5

(99) Immunogenicity and Cross-Protection Efficacy of a Genotype F-Derived Attenuated Virus Vaccine Candidate against Mumps Virus in MiceVaccines (Basel)May 30, 2024Seo-Yeon Kim, Hyeran Won, Yun-Ho Hwang, Se-Eun Kim, Jung-Ah Lee, Dokeun Kim, You-Jin Kim, Tae-Young Leeresidual viral RNA was extracted using a Maxwell RSC 48 Instrument (Promega, Madison, WI, USA; Cat#: AS8500) and a Maxwell RSC Viral Total Nucleic Acid Purification Kit (Promega, Madison, WI, USA; Cat#: AS1330)

(100) Unveiling the genomic landscape of Salmonella enterica serotypes Typhimurium, Newport, and Infantis in Latin American surface waters: a comparative analysisMicrobiology SpectrumMay 2, 2024Zhao Chen, Magaly Toro, Andrea I. Moreno-Switt, Aiko D. Adell, Enrique J. Delgado-Suárez, Raquel R. Bonelli, Celso J. B. Oliveira, Angélica Reyes-Jara, Xinyang Huang, Brett Albee, et al.was extracted using the Maxwell RSC cultured cells DNA kit (Promega Corporation, Madison, WI) on the Maxwell RSC 48 instrument (Promega Corporation). Once extracted, the genomic DNA samples were stored at 4°C until they were ready

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