Maxwell® RSC 48 Instrument from Promega

Product Specs
ItemMaxwell® RSC 48 Instrument
CompanyPromega
Price Supplier Page
Catalog NumberAS8500
QuantityEA

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(81) The Influence of β-Carotene and Its Liposomal Form on the Expression of EMT Markers and Androgen-Dependent Pathways in Different Prostate Cell LinesAntioxidants (Basel)July 25, 2024Joanna Dulińska-Litewka, Kacper Dykas, Stanisław Boznański, Przemysław Hałubiec, Marta Kaczor-Kamińska, Jacek Zagajewski, Torsten Bohn, Gracjan Wątor(Promega, Madison, WI, USA). The procedure applied paramagnetic particles (as a mobile solid phase) and the Maxwell RSC 48 Instrument (Promega, Madison, WI, USA). The concentration and purity of RNA were determined with the use of Nanodrop

(82) A multicenter genomic epidemiological investigation in Brazil, Chile, and Mexico reveals the diversity and persistence of Salmonella populations in surface watersmBioJuly 17, 2024Zhao Chen, Andrea I. Moreno-Switt, Angelica Reyes-Jara, Enrique Delgado Suarez, Aiko D. Adell, Celso José Bruno Oliveira, Raquel Regina Bonelli, Xinyang Huang, Eric Brown, Marc Allard, et al.individual colonies were grown overnight in TSB broth (BD, Sparks, MD, USA) at 37°C. DNA was purified on a Maxwell RSC-48 Instrument using the Cultured Cells DNA Kit (Promega, Madison, WI, USA). Libraries were prepared with the Illumina

(83) Cerebrospinal Fluid cfDNA Sequencing for Classification of Central Nervous System GliomaClinical Cancer ResearchJuly 15, 2024Florian Iser, Felix Hinz, Dirk C. Hoffmann, Niklas Grassl, Cansu Güngoör, Jochen Meyer, Laura Dörner, Lea Hofmann, Vanessa Kelbch, Kirsten Göbel, et al.formalin-fixed paraffin-embedded (FFPE) tissue using the Maxwell RSC Buffy Coat DNA Kit and Maxwell RSC DNA FFPE Kit on a Maxwell RSC 48 instrument (Promega) according to the manufacturer’s recommendations. Preparation of NGS libraries For gDNA derived

(84) SGLT2 inhibition eliminates senescent cells and alleviates pathological agingNat AgingJuly 1, 2024Katsuumi G, Shimizu I, Suda M, Yoshida Y, Furihata T, Joki Y, Hsiao CL, Jiaqi L, Fujiki S, Abe M, et al.and QUantiStudio6 Pro. RNA sequencing analysis Total RNA was extracted from mouse gWAT using the Maxwell RSC 48 Instrument (Promega) and Maxwell RSC simplyRNA Tissue Kit (Promega, cat. no. AS1340) in accordance with the manufacturer’s

(85) Detection of hidden antibiotic resistance through real-time genomicsNature CommunicationsJune 28, 2024Ela Sauerborn, Nancy Carolina Corredor, Tim Reska, Albert Perlas, Samir Vargas da Fonseca Atum, Nick Goldman, Nina Wantia, Clarissa Prazeres da Costa, Ebenezer Foster-Nyarko, Lara Urbanthrough the Maxwell® RSC Blood DNA extraction protocol for Gram-negative bacteria with the Promega Maxwell®RSC 48 Instrument (Promega Corporation, Madison, USA). DNA concentrations were measured using the QubitTM (Thermo Fischer

(86) Latin American Trans‐ancestry INitiative for OCD genomics (LATINO): Study protocolAmerican Journal of Medical Genetics Part B: Neuropsychiatric GeneticsJune 1, 2024James J. Crowley, Carolina Cappi, Marcos E. Ochoa‐Panaifo, Renee M. Frederick, Minjee Kook, Andrew D. Wiese, Diana Rancourt, Elizabeth G. Atkinson, Paola Giusti‐Rodriguez, Jacey L. Anderberg, et al.of saliva is extracted in an automated fashion using Maxwell® RSC Stabilized Saliva DNA Kits and a Maxwell® RSC 48 Instrument from Promega (Madison, Wisconsin, USA). The remaining saliva solution is frozen at −80 degrees C. 2.1.5

(87) Immunogenicity and Cross-Protection Efficacy of a Genotype F-Derived Attenuated Virus Vaccine Candidate against Mumps Virus in MiceVaccines (Basel)May 30, 2024Seo-Yeon Kim, Hyeran Won, Yun-Ho Hwang, Se-Eun Kim, Jung-Ah Lee, Dokeun Kim, You-Jin Kim, Tae-Young Leeresidual viral RNA was extracted using a Maxwell RSC 48 Instrument (Promega, Madison, WI, USA; Cat#: AS8500) and a Maxwell RSC Viral Total Nucleic Acid Purification Kit (Promega, Madison, WI, USA; Cat#: AS1330)

(88) Unveiling the genomic landscape of Salmonella enterica serotypes Typhimurium, Newport, and Infantis in Latin American surface waters: a comparative analysisMicrobiology SpectrumMay 2, 2024Zhao Chen, Magaly Toro, Andrea I. Moreno-Switt, Aiko D. Adell, Enrique J. Delgado-Suárez, Raquel R. Bonelli, Celso J. B. Oliveira, Angélica Reyes-Jara, Xinyang Huang, Brett Albee, et al.was extracted using the Maxwell RSC cultured cells DNA kit (Promega Corporation, Madison, WI) on the Maxwell RSC 48 instrument (Promega Corporation). Once extracted, the genomic DNA samples were stored at 4°C until they were ready

(89) Head and neck cancer of unknown primary: unveiling primary tumor sites through machine learning on DNA methylation profilesClin EpigeneticsMarch 25, 2024Leonhard Stark, Atsuko Kasajima, Fabian Stögbauer, Benedikt Schmidl, Jakob Rinecker, Katharina Holzmann, Sarah Färber, Nicole Pfarr, Katja Steiger, Barbara Wollenberg, et al.macrodissecting these marked areas, DNA was extracted using the Maxwell RSC Blood DNA Kit with the Maxwell RSC 48 instrument (Promega). For DNA quality control, fragmentation and integrity were evaluated using the 4200 TapeStation

(90) CA-CAS-01-A: A Permissive Cell Line for Isolation and Live Attenuated Vaccine Development Against African Swine Fever VirusJournal of MicrobiologyMarch 13, 2024Seung-Chul Lee, Yongkwan Kim, Ji-Won Cha, Kiramage Chathuranga, Niranjan Dodantenna, Hyeok-Il Kwon, Min Ho Kim, Weonhwa Jheong, In-Joong Yoon, Joo Young Lee, et al.Next, the mixture was heated at 56 °C for 30 min. Viral DNA was extracted in 50 μl increments using the Maxwell® RSC 48 Instrument in the Maxwell RSC Whole Blood DNA Kit (Promega, AS1520) according to the manufacturer's instructions. Real-Time Polymerase Chain Reaction

(91) Disease Course of Korean African Swine Fever Virus in Domestic Pigs Exposed Intraorally, Intranasally, Intramuscularly, and by Direct Contact with Infected PigsVirusesMarch 11, 2024Ki-Hyun Cho, Seong-Keun Hong, Da-Young Kim, Hyun-Joo Sohn, Dae-Sung Yoo, Hae-Eun Kang, Yeon-Hee KimAntigen and Antibody Detection Viral nucleic acids were extracted from all collected samples using the Maxwell® RSC 48 instrument (Promega, Madison, WI, USA) according to the manufacturer’s instructions. The Maxwell® RSC Whole Blood DNA kit

(92) Histone butyrylation in the mouse intestine is mediated by the microbiota and associated with regulation of gene expressionNature MetabolismFebruary 27, 2024Leah A. Gates, Bernardo Sgarbi Reis, Peder J. Lund, Matthew R. Paul, Marylene Leboeuf, Annaelle M. Djomo, Zara Nadeem, Mariana Lopes, Francisca N. Vitorino, Gokhan Unlu, et al.Weill Cornell Medicine23. Cecal contents were subject to DNA extraction using the Max Prep and the Maxwell RSC 48 Instrument (Promega). Primers targeting the V4 and V5 regions (515F-926R) were used for amplification according

(93) Antimicrobial peptide glatiramer acetate targets Pseudomonas aeruginosa lipopolysaccharides to breach membranes without altering lipopolysaccharide modificationnpj Antimicrobials and ResistanceFebruary 20, 2024Ronan A. Murphy, Jade Pizzato, Leah Cuthbertson, Akshay Sabnis, Andrew M. Edwards, Laura M. Nolan, Thomas Vorup-Jensen, Gerald Larrouy-Maumus, Jane C. Daviessequencing were performed using Maxwell™ RSC Cell DNA Purification Kit and automated extraction on the Maxwell RSC 48 Instrument (Promega). The DNA concentration was evaluated using a Qubit dsDNA BR assay kit and Qubit Fluorometer

(94) Cerebrospinal Fluid cfDNA Sequencing for Classification of Central Nervous System GliomaClin Cancer ResJanuary 31, 2024Florian Iser, Felix Hinz, Dirk C. Hoffmann, Niklas Grassl, Cansu Güngoör, Jochen Meyer, Laura Dörner, Lea Hofmann, Vanessa Kelbch, Kirsten Göbel, et al.formalin-fixed paraffin-embedded (FFPE) tissue using the Maxwell RSC Buffy Coat DNA Kit and Maxwell RSC DNA FFPE Kit on a Maxwell RSC 48 instrument (Promega) according to the manufacturer’s recommendations. Preparation of NGS libraries For gDNA derived

(95) Community-wide versus school-based targeted deworming for soil-transmitted helminth control in school-aged children in Vietnam: the CoDe-STH cluster-randomised controlled trialThe Lancet Regional Health - Western PacificDecember 1, 2023Clare E.F. Dyer, Dinh Ng-Nguyen, Naomi E. Clarke, Sze Fui Hii, Hieu Quoc Nguyen, Van-Anh Thi Nguyen, Thai Van Nguyen, Trong Van Nguyen, Tam Thi Ngo, H.M.P. Dilrukshi Herath, et al.steps, DNA extraction was performed using the Maxwell® RSC PureFood GMO and Authentication Kit on a Maxwell® RSC 48 Instrument (Promega Corporation). Multiplex PCR assays were then used to detect and quantify N. americanus, A.

(96) APOBEC3G Is a p53-Dependent Restriction Factor in Respiratory Syncytial Virus Infection of Human Cells Included in the p53/Immune AxisInternational Journal of Molecular SciencesNovember 27, 2023Wesley Gladwell, Oriana Yost, Heather Li, Whitney J. Bell, Shih-Heng Chen, James M. Ward, Steven R. Kleeberger, Michael A. Resnick, Daniel MenendezDigital PCR (ddPCR) Total RNA was isolated with Promega Maxwell RSC simplyRNA cell kits using a Promega Maxwell RSC 48 instrument following the manufacturer’s recommendations. Then, 500 ng total RNA was reverse-transcribed using an

(97) Efficient multiple gene knockout in Colletotrichum higginsianum via CRISPR/Cas9 ribonucleoprotein and URA3‐based marker recyclingMolecular Plant PathologyNovember 1, 2023Katsuma Yonehara, Naoyoshi Kumakura, Takayuki Motoyama, Nobuaki Ishihama, Jean‐Félix Dallery, Richard O'Connell, Ken Shirasuhigginsianum genomic DNA used as PCR templates was isolated by the Maxwell RSC Plant DNA Kit with a Maxwell RSC 48 instrument (Promega) following the manufacturer's protocol. PCRs were performed using KOD One PCR Master Mix ‐Blue‐

(98) Rapid and strain-specific resistance evolution of Staphylococcus aureus against inhibitory molecules secreted by Pseudomonas aeruginosamBioOctober 31, 2023Selina Niggli, Lukas Schwyter, Lucy Poveda, Jonas Grossmann, Rolf KümmerliWe used the Maxwell RSC Cultured Cells DNA Kit (Promega, Dübendorf, Switzerland) together with the Maxwell RSC 48 instrument (Promega) following the manufacturer’s protocol. To lyse the gram-positive SA cells, we added lysostaphin

(99) Assessing residential activity in a home plumbing system simulator: monitoring the occurrence and relationship of major opportunistic pathogens and phagocytic amoebasFrontiers in MicrobiologyOctober 17, 2023Vicente Gomez-Alvarez, Hodon Ryu, Min Tang, Morgan McNeely, Christy Muhlen, Megan Urbanic, Daniel Williams, Darren Lytle, Laura BoczekDNA was extracted using the PureFood GMO and Authentication cartridge-based purification kit with the Maxwell® RSC 48 Instrument (Promega, Madison, WI) following the manufacturer’s instructions. Briefly, Filters and swabs were resuspended

(100) Molecular landscape and functional characterization of centrosome amplification in ovarian cancerNature CommunicationsOctober 16, 2023Carolin M. Sauer, James A. Hall, Dominique-Laurent Couturier, Thomas Bradley, Anna M. Piskorz, Jacob Griffiths, Ashley Sawle, Matthew D. Eldridge, Philip Smith, Karen Hosking, et al.and RNA Kit (Promega, AS1620 and AS1390, respectively) with the Maxwell® RSC 48 Instrument (Promega, AS8500). DNA sequencing Tagged-Amplicon Sequencing (TAmSeq) Extracted DNA samples were diluted to a final

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