Maxwell® RSC 48 Instrument from Promega

Product Specs
ItemMaxwell® RSC 48 Instrument
CompanyPromega
Price Supplier Page
Catalog NumberAS8500
QuantityEA

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(101) Head and neck cancer of unknown primary: unveiling primary tumor sites through machine learning on DNA methylation profilesClin EpigeneticsMarch 25, 2024Leonhard Stark, Atsuko Kasajima, Fabian Stögbauer, Benedikt Schmidl, Jakob Rinecker, Katharina Holzmann, Sarah Färber, Nicole Pfarr, Katja Steiger, Barbara Wollenberg, et al.macrodissecting these marked areas, DNA was extracted using the Maxwell RSC Blood DNA Kit with the Maxwell RSC 48 instrument (Promega). For DNA quality control, fragmentation and integrity were evaluated using the 4200 TapeStation

(102) CA-CAS-01-A: A Permissive Cell Line for Isolation and Live Attenuated Vaccine Development Against African Swine Fever VirusJournal of MicrobiologyMarch 13, 2024Seung-Chul Lee, Yongkwan Kim, Ji-Won Cha, Kiramage Chathuranga, Niranjan Dodantenna, Hyeok-Il Kwon, Min Ho Kim, Weonhwa Jheong, In-Joong Yoon, Joo Young Lee, et al.Next, the mixture was heated at 56 °C for 30 min. Viral DNA was extracted in 50 μl increments using the Maxwell® RSC 48 Instrument in the Maxwell RSC Whole Blood DNA Kit (Promega, AS1520) according to the manufacturer's instructions. Real-Time Polymerase Chain Reaction

(103) Disease Course of Korean African Swine Fever Virus in Domestic Pigs Exposed Intraorally, Intranasally, Intramuscularly, and by Direct Contact with Infected PigsVirusesMarch 11, 2024Ki-Hyun Cho, Seong-Keun Hong, Da-Young Kim, Hyun-Joo Sohn, Dae-Sung Yoo, Hae-Eun Kang, Yeon-Hee KimAntigen and Antibody Detection Viral nucleic acids were extracted from all collected samples using the Maxwell® RSC 48 instrument (Promega, Madison, WI, USA) according to the manufacturer’s instructions. The Maxwell® RSC Whole Blood DNA kit

(104) Histone butyrylation in the mouse intestine is mediated by the microbiota and associated with regulation of gene expressionNature MetabolismFebruary 27, 2024Leah A. Gates, Bernardo Sgarbi Reis, Peder J. Lund, Matthew R. Paul, Marylene Leboeuf, Annaelle M. Djomo, Zara Nadeem, Mariana Lopes, Francisca N. Vitorino, Gokhan Unlu, et al.Weill Cornell Medicine23. Cecal contents were subject to DNA extraction using the Max Prep and the Maxwell RSC 48 Instrument (Promega). Primers targeting the V4 and V5 regions (515F-926R) were used for amplification according

(105) Antimicrobial peptide glatiramer acetate targets Pseudomonas aeruginosa lipopolysaccharides to breach membranes without altering lipopolysaccharide modificationnpj Antimicrobials and ResistanceFebruary 20, 2024Ronan A. Murphy, Jade Pizzato, Leah Cuthbertson, Akshay Sabnis, Andrew M. Edwards, Laura M. Nolan, Thomas Vorup-Jensen, Gerald Larrouy-Maumus, Jane C. Daviessequencing were performed using Maxwell™ RSC Cell DNA Purification Kit and automated extraction on the Maxwell RSC 48 Instrument (Promega). The DNA concentration was evaluated using a Qubit dsDNA BR assay kit and Qubit Fluorometer

(106) Cerebrospinal Fluid cfDNA Sequencing for Classification of Central Nervous System GliomaClin Cancer ResJanuary 31, 2024Florian Iser, Felix Hinz, Dirk C. Hoffmann, Niklas Grassl, Cansu Güngoör, Jochen Meyer, Laura Dörner, Lea Hofmann, Vanessa Kelbch, Kirsten Göbel, et al.formalin-fixed paraffin-embedded (FFPE) tissue using the Maxwell RSC Buffy Coat DNA Kit and Maxwell RSC DNA FFPE Kit on a Maxwell RSC 48 instrument (Promega) according to the manufacturer’s recommendations. Preparation of NGS libraries For gDNA derived

(107) Community-wide versus school-based targeted deworming for soil-transmitted helminth control in school-aged children in Vietnam: the CoDe-STH cluster-randomised controlled trialThe Lancet Regional Health - Western PacificDecember 1, 2023Clare E.F. Dyer, Dinh Ng-Nguyen, Naomi E. Clarke, Sze Fui Hii, Hieu Quoc Nguyen, Van-Anh Thi Nguyen, Thai Van Nguyen, Trong Van Nguyen, Tam Thi Ngo, H.M.P. Dilrukshi Herath, et al.steps, DNA extraction was performed using the Maxwell® RSC PureFood GMO and Authentication Kit on a Maxwell® RSC 48 Instrument (Promega Corporation). Multiplex PCR assays were then used to detect and quantify N. americanus, A.

(108) APOBEC3G Is a p53-Dependent Restriction Factor in Respiratory Syncytial Virus Infection of Human Cells Included in the p53/Immune AxisInternational Journal of Molecular SciencesNovember 27, 2023Wesley Gladwell, Oriana Yost, Heather Li, Whitney J. Bell, Shih-Heng Chen, James M. Ward, Steven R. Kleeberger, Michael A. Resnick, Daniel MenendezDigital PCR (ddPCR) Total RNA was isolated with Promega Maxwell RSC simplyRNA cell kits using a Promega Maxwell RSC 48 instrument following the manufacturer’s recommendations. Then, 500 ng total RNA was reverse-transcribed using an

(109) Efficient multiple gene knockout in Colletotrichum higginsianum via CRISPR/Cas9 ribonucleoprotein and URA3‐based marker recyclingMolecular Plant PathologyNovember 1, 2023Katsuma Yonehara, Naoyoshi Kumakura, Takayuki Motoyama, Nobuaki Ishihama, Jean‐Félix Dallery, Richard O'Connell, Ken Shirasuhigginsianum genomic DNA used as PCR templates was isolated by the Maxwell RSC Plant DNA Kit with a Maxwell RSC 48 instrument (Promega) following the manufacturer's protocol. PCRs were performed using KOD One PCR Master Mix ‐Blue‐

(110) Rapid and strain-specific resistance evolution of Staphylococcus aureus against inhibitory molecules secreted by Pseudomonas aeruginosamBioOctober 31, 2023Selina Niggli, Lukas Schwyter, Lucy Poveda, Jonas Grossmann, Rolf KümmerliWe used the Maxwell RSC Cultured Cells DNA Kit (Promega, Dübendorf, Switzerland) together with the Maxwell RSC 48 instrument (Promega) following the manufacturer’s protocol. To lyse the gram-positive SA cells, we added lysostaphin

(111) Assessing residential activity in a home plumbing system simulator: monitoring the occurrence and relationship of major opportunistic pathogens and phagocytic amoebasFrontiers in MicrobiologyOctober 17, 2023Vicente Gomez-Alvarez, Hodon Ryu, Min Tang, Morgan McNeely, Christy Muhlen, Megan Urbanic, Daniel Williams, Darren Lytle, Laura BoczekDNA was extracted using the PureFood GMO and Authentication cartridge-based purification kit with the Maxwell® RSC 48 Instrument (Promega, Madison, WI) following the manufacturer’s instructions. Briefly, Filters and swabs were resuspended

(112) Molecular landscape and functional characterization of centrosome amplification in ovarian cancerNature CommunicationsOctober 16, 2023Carolin M. Sauer, James A. Hall, Dominique-Laurent Couturier, Thomas Bradley, Anna M. Piskorz, Jacob Griffiths, Ashley Sawle, Matthew D. Eldridge, Philip Smith, Karen Hosking, et al.and RNA Kit (Promega, AS1620 and AS1390, respectively) with the Maxwell® RSC 48 Instrument (Promega, AS8500). DNA sequencing Tagged-Amplicon Sequencing (TAmSeq) Extracted DNA samples were diluted to a final

(113) QTLs and Candidate Genes for Seed Protein Content in Two Recombinant Inbred Line Populations of SoybeanPlants (Basel)October 16, 2023Hye Rang Park, Jeong Hyun Seo, Beom Kyu Kang, Jun Hoi Kim, Su Vin Heo, Man Soo Choi, Jee Yeon Ko, Choon Song Kimextracted from dry seeds of each line of the mapping populations and the three parental lines using a Maxwell RSC 48 instrument (Promega Madison, WI, USA), following the manufacturer’s instructions. The DNA quality was assessed

(114) Wolbachia-mediated resistance to Zika virus infection in Aedes aegypti is dominated by diverse transcriptional regulation and weak evolutionary pressuresPLOS Neglected Tropical DiseasesOctober 2, 2023Emma C. Boehm, Anna S. Jaeger, Hunter J. Ries, David Castañeda, Andrea M. Weiler, Corina C. Valencia, James Weger-Lucarelli, Gregory D. Ebel, Shelby L. O’Connor, Thomas C. Friedrich, et al.RNA isolation. RNA was isolated with the Maxwell RSC viral total nucleic acid purification kit on a Maxwell RSC 48 instrument (Promega). Isolated ZIKV RNA was titrated by qRT-PCR using TaqMan Fast virus 1-step master mix (ThermoFisher)

(115) Independent COL17A1 Variants in Cats with Junctional Epidermolysis BullosaGenes (Basel)September 22, 2023Sarah Kiener, Heather Troyer, Daniel Ruvolo, Paula Grest, Sara Soto, Anna Letko, Vidhya Jagannathan, Tosso Leeb, Elizabeth A. Mauldin, Ching Yang, et al.mammary carcinoma. 2.4. DNA Isolation and Whole Genome Sequencing DNA was isolated from EDTA blood on a Maxwell RSC 48 instrument using the Maxwell RSC Whole Blood DNA Kit (Promega, Dübendorf, Switzerland). The genomes of both affected

(116) Identification of novel genetic risk factors of dilated cardiomyopathy: from canine to humanGenome MedicineSeptember 18, 2023Julia E. Niskanen, Åsa Ohlsson, Ingrid Ljungvall, Michaela Drögemüller, Robert F. Ernst, Dennis Dooijes, Hanneke W. M. van Deutekom, J. Peter van Tintelen, Christian J. B. Snijders Blok, Marion van Vugt, et al.a semi-automated Chemagen extraction robot (PerkinElmer Chemagen Technologie, Waltham, MA, USA), a Maxwell RSC 48 instrument with the Maxwell RSC Whole Blood DNA Kit (Promega, Madison, WI, USA), a Nucleon BACC2 kit (GE Healthcare,

(117) An Integrated Coproscopic and Molecular Method Provides Insights into the Epidemiology of Zoonotic Intestinal Helminths of Dogs across CambodiaTransboundary and Emerging DiseasesAugust 22, 2023Patsy A. Zendejas-Heredia, Vito Colella, Lucas G. Huggins, Roland Schaper, Bettina Schunack, Rebecca J. TraubInstrument (MP Biomedicals). After bead-beating and cell lysis, DNA purification was performed using a Maxwell RSC 48 Instrument (Promega). Final eluted DNA (100 μL) was stored at −20°C until further analyses. Extracted DNA was subjected

(118) CD137 (4-1BB) requires physically associated cIAPs for signal transduction and antitumor effectsScience AdvancesAugust 18, 2023Javier Glez-Vaz, Arantza Azpilikueta, María C. Ochoa, Irene Olivera, Gabriel Gomis, Asunta Cirella, Carlos Luri-Rey, Maite Álvarez, Jose L. Pérez-Gracia, Sergio Ciordia, et al.Jurkat cells and activated human CD8 T cells using a Maxwell RSC simplyRNA tissue kit (Promega) in a Maxwell RSC 48 instrument. M-MLV reverse transcriptase (Invitrogen) and random primers (Invitrogen) were used to obtain cDNA.

(119) Einkorn genomics sheds light on history of the oldest domesticated wheatNatureAugust 2, 2023Hanin Ibrahim Ahmed, Matthias Heuberger, Adam Schoen, Dal-Hoe Koo, Jesus Quiroz-Chavez, Laxman Adhikari, John Raupp, Stéphane Cauet, Nathalie Rodde, Charlotte Cravero, et al.glumes and grains were used for RNA isolation using the Maxwell RSC Plant RNA Kit (AS1500) and the Maxwell RSC 48 instrument according to the kit protocol (Promega). For RNA-seq, around 10 Gb of Illumina 150 bp paired-end reads

(120) bettercallsal: better calling of Salmonella serotypes from enrichment cultures using shotgun metagenomic profiling and its application in an outbreak settingFront MicrobiolAugust 2, 2023Kranti Konganti, Elizabeth Reed, Mark Mammel, Tunc Kayikcioglu, Rachel Binet, Karen Jarvis, Christina M. Ferreira, Rebecca L. Bell, Jie Zheng, Amanda M. Windsor, et al.Maxwell® RSC Cultured Cells DNA Kit (Promega, WI, USA, AS1620) according to the manufacturer's specifications on the Promega Maxwell® RSC 48 instrument. Shotgun metagenomic profiling was performed on the DNA extracted from the selective enrichments as

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