Maxwell® RSC 48 Instrument from Promega

Product Specs
ItemMaxwell® RSC 48 Instrument
CompanyPromega
Price Supplier Page
Catalog NumberAS8500
QuantityEA

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(101) QTLs and Candidate Genes for Seed Protein Content in Two Recombinant Inbred Line Populations of SoybeanPlants (Basel)October 16, 2023Hye Rang Park, Jeong Hyun Seo, Beom Kyu Kang, Jun Hoi Kim, Su Vin Heo, Man Soo Choi, Jee Yeon Ko, Choon Song Kimextracted from dry seeds of each line of the mapping populations and the three parental lines using a Maxwell RSC 48 instrument (Promega Madison, WI, USA), following the manufacturer’s instructions. The DNA quality was assessed

(102) Wolbachia-mediated resistance to Zika virus infection in Aedes aegypti is dominated by diverse transcriptional regulation and weak evolutionary pressuresPLOS Neglected Tropical DiseasesOctober 2, 2023Emma C. Boehm, Anna S. Jaeger, Hunter J. Ries, David Castañeda, Andrea M. Weiler, Corina C. Valencia, James Weger-Lucarelli, Gregory D. Ebel, Shelby L. O’Connor, Thomas C. Friedrich, et al.RNA isolation. RNA was isolated with the Maxwell RSC viral total nucleic acid purification kit on a Maxwell RSC 48 instrument (Promega). Isolated ZIKV RNA was titrated by qRT-PCR using TaqMan Fast virus 1-step master mix (ThermoFisher)

(103) Independent COL17A1 Variants in Cats with Junctional Epidermolysis BullosaGenes (Basel)September 22, 2023Sarah Kiener, Heather Troyer, Daniel Ruvolo, Paula Grest, Sara Soto, Anna Letko, Vidhya Jagannathan, Tosso Leeb, Elizabeth A. Mauldin, Ching Yang, et al.mammary carcinoma. 2.4. DNA Isolation and Whole Genome Sequencing DNA was isolated from EDTA blood on a Maxwell RSC 48 instrument using the Maxwell RSC Whole Blood DNA Kit (Promega, Dübendorf, Switzerland). The genomes of both affected

(104) Identification of novel genetic risk factors of dilated cardiomyopathy: from canine to humanGenome MedicineSeptember 18, 2023Julia E. Niskanen, Åsa Ohlsson, Ingrid Ljungvall, Michaela Drögemüller, Robert F. Ernst, Dennis Dooijes, Hanneke W. M. van Deutekom, J. Peter van Tintelen, Christian J. B. Snijders Blok, Marion van Vugt, et al.a semi-automated Chemagen extraction robot (PerkinElmer Chemagen Technologie, Waltham, MA, USA), a Maxwell RSC 48 instrument with the Maxwell RSC Whole Blood DNA Kit (Promega, Madison, WI, USA), a Nucleon BACC2 kit (GE Healthcare,

(105) An Integrated Coproscopic and Molecular Method Provides Insights into the Epidemiology of Zoonotic Intestinal Helminths of Dogs across CambodiaTransboundary and Emerging DiseasesAugust 22, 2023Patsy A. Zendejas-Heredia, Vito Colella, Lucas G. Huggins, Roland Schaper, Bettina Schunack, Rebecca J. TraubInstrument (MP Biomedicals). After bead-beating and cell lysis, DNA purification was performed using a Maxwell RSC 48 Instrument (Promega). Final eluted DNA (100 μL) was stored at −20°C until further analyses. Extracted DNA was subjected

(106) CD137 (4-1BB) requires physically associated cIAPs for signal transduction and antitumor effectsScience AdvancesAugust 18, 2023Javier Glez-Vaz, Arantza Azpilikueta, María C. Ochoa, Irene Olivera, Gabriel Gomis, Asunta Cirella, Carlos Luri-Rey, Maite Álvarez, Jose L. Pérez-Gracia, Sergio Ciordia, et al.Jurkat cells and activated human CD8 T cells using a Maxwell RSC simplyRNA tissue kit (Promega) in a Maxwell RSC 48 instrument. M-MLV reverse transcriptase (Invitrogen) and random primers (Invitrogen) were used to obtain cDNA.

(107) Einkorn genomics sheds light on history of the oldest domesticated wheatNatureAugust 2, 2023Hanin Ibrahim Ahmed, Matthias Heuberger, Adam Schoen, Dal-Hoe Koo, Jesus Quiroz-Chavez, Laxman Adhikari, John Raupp, Stéphane Cauet, Nathalie Rodde, Charlotte Cravero, et al.glumes and grains were used for RNA isolation using the Maxwell RSC Plant RNA Kit (AS1500) and the Maxwell RSC 48 instrument according to the kit protocol (Promega). For RNA-seq, around 10 Gb of Illumina 150 bp paired-end reads

(108) bettercallsal: better calling of Salmonella serotypes from enrichment cultures using shotgun metagenomic profiling and its application in an outbreak settingFront MicrobiolAugust 2, 2023Kranti Konganti, Elizabeth Reed, Mark Mammel, Tunc Kayikcioglu, Rachel Binet, Karen Jarvis, Christina M. Ferreira, Rebecca L. Bell, Jie Zheng, Amanda M. Windsor, et al.Maxwell® RSC Cultured Cells DNA Kit (Promega, WI, USA, AS1620) according to the manufacturer's specifications on the Promega Maxwell® RSC 48 instrument. Shotgun metagenomic profiling was performed on the DNA extracted from the selective enrichments as

(109) APC-Related Phenotypes and Intellectual Disability in 5q Interstitial Deletions: A New Case and Review of the LiteratureGenes (Basel)July 23, 2023Flavia Privitera, Flavia Piccini, Maria Paola Recalcati, Silvia Presi, Silvia Mazzola, Paola Carreradeclaration of Helsinki. Genomic DNA was isolated and extracted from EDTA peripheral blood samples using a Maxwell® RSC 48 Instrument and the Maxwell® RSC Whole Blood DNA kit (Promega, Madison, WI, USA), according to the manufacturer’s

(110) A Comparative Analysis of NOX4 Protein Expression in Malignant and Non-Malignant Thyroid TumorsCurrent Issues in Molecular BiologyJuly 13, 2023Salma Fenniche, Mohamed Oukabli, Yassire Oubaddou, Hafsa Chahdi, Amal Damiri, Abir Alghuzlan, Abdelilah Laraqui, Nadia Dakka, Youssef Bakri, Corinne Dupuy, et al.extracted using the ‘Maxwell® RSC DNA FFPE Kit’ from Promega/‘Maxwell® RSC Instruments (Cat.# AS4500, AS8500)’ according to the manufacturer’s protocols (ASB1450). The extracted genomic DNA was sequenced using

(111) An efficient method for high molecular weight bacterial DNA extraction suitable for shotgun metagenomics from skin swabsMicrobial GenomicsJuly 10, 2023Iliana R. Serghiou, Dave Baker, Rhiannon Evans, Matthew J. Dalby, Raymond Kiu, Eleftheria Trampari, Sarah Phillips, Rachel Watt, Thomas Atkinson, Barry Murphy, et al.diluents and lysis procedures were evaluated for effectiveness before DNA was extracted using a Promega Maxwell RSC 48 Instrument and RSC Blood DNA Kit (see File S1, available in the online version of this article for protocol). This

(112) SOAT1 missense variant in two cats with sebaceous gland dysplasiaMolecular Genetics and GenomicsJuly 1, 2023Sarah Kiener, Barbara G. McMahill, Verena K. Affolter, Monika Welle, Julie A. Yager, Vidhya Jagannathan, Tosso LeebMaxwell® RSC PureFood GMO and Authentication Kit, or Maxwell® RSC DNA FFPE Kit, respectively, on a Maxwell® RSC 48 instrument (Promega, Dübendorf, Switzerland). Whole genome sequencing and variant calling The genome of both

(113) Wolbachia -mediated resistance to Zika virus infection in Aedes aegypti is dominated by diverse transcriptional regulation and weak evolutionary pressuresbioRxivJuly 1, 2023Emma C. Boehm, Anna S. Jaeger, Hunter J. Ries, David Castañeda, Andrea M. Weiler, Corina C. Valencia, James Weger-Lucarelli, Gregory D. Ebel, Shelby L. O’Connor, Thomas C. Friedrich, et al.RNA isolation. RNA was isolated with the Maxwell RSC viral total nucleic acid purification kit on a Maxwell RSC 48 instrument (Promega). Isolated ZIKV RNA was titrated by qRT-PCR using TaqMan Fast virus 1-step master mix (ThermoFisher)

(114) Epidemiology of soil-transmitted helminths using quantitative PCR and risk factors for hookworm and Necator americanus infection in school children in Dak Lak province, VietnamParasites & VectorsJune 27, 2023Angus Hughes, Dinh Ng-Nguyen, Naomi E. Clarke, Clare E. F. Dyer, Sze Fui Hii, Archie C. A. Clements, Roy M. Anderson, Darren J. Gray, Luc E. Coffeng, John M. Kaldor, et al.27, 28]. DNA extraction was performed using a Maxwell RSC PureFood GMO and Authentication Kit and a Maxwell RSC 48 Instrument (Promega Corporation, USA). Two probe-based real-time multiplex qPCR assays were used to detect infection

(115) Antisense oligonucleotides targeting hepatic angiotensinogen reduce atherosclerosis and liver steatosis in hypercholesterolemic miceGlobal Translational MedicineJune 9, 2023Dien Ye, Congqing Wu, Lei Cai, Deborah A. Howatt, Ching-Ling Liang, Yuriko Katsumata, Adam E. Mullick, Ryan E. Temel, A.H. Jan Danser, Alan Daugherty, et al.extracted from liver and kidney samples using a commercial kit (Cat # AS1280, Promega) and the automated Maxwell® RSC 48 Instrument (Promega). To quantify mRNA abundance, total RNA was reversely transcribed with iScript™ cDNA Synthesis kit

(116) Whole-genome sequencing and phylogenetic analysis capture the emergence of a multi-drug resistant Salmonella enterica serovar Infantis clone from diagnostic animal samples in the United StatesFrontiers in MicrobiologyJune 2, 2023Mariela E. Srednik, Brenda R. Morningstar-Shaw, Jessica A. Hicks, Christopher Tong, Tonya A. Mackie, Linda K. SchlaterSystem (Thermo Fisher Scientific, Waltham, MA, USA); and nucleic acid was extracted using the Promega Maxwell® RSC 48 Instrument (Madison, WI). Isolates were sequenced with a targeted depth of 40-80X using 2 × 250 paired end chemistry

(117) Comparison of NTRK fusion detection methods in microsatellite-instability-high metastatic colorectal cancerVirchows ArchivJune 1, 2023Suzanna J. Schraa, Ellen Stelloo, Miangela M. Laclé, Joost F. Swennenhuis, Lodewijk A. A. Brosens, Remond J. A. Fijneman, Harma Feitsma, Miriam Koopman, Wendy W. de Leng, Geraldine R. Vink, et al.assess NTRK1, NTRK2, and NTRK3 rearrangements (Supplementary Table S1). RNA was isolated using the Maxwell® RSC 48 instrument and the Maxwell® RSC RNA FFPE kit (Promega, Madison, USA) from four FFPE sections of 10 µm with a minimum

(118) Linking Pregnancy and Long-Term Health: The Impact of Cardiovascular Risk on Telomere Shortening in Pregnant WomenMedicina (Kaunas)May 24, 2023Simona-Alina Abu-Awwad, Marius Craina, Adrian Gluhovschi, Paula Diana Ciordas, Catalin Marian, Lioara Boscu, Elena Bernad, Mircea Iurciuc, Ahmed Abu-Awwad, Stela Iurciuc, et al.which was carried out using the Maxwell® RSC Buffy Coat DNA Kit (Promega Corp, Madison, WI, USA) in a Maxwell® RSC 48 Instrument (Promega Corp, Madison, WI, USA) according to the manufacturer’s instructions. The telomere length was

(119) A draft human pangenome referenceNatureMay 11, 2023Wen-Wei Liao, Mobin Asri, Jana Ebler, Daniel Doerr, Marina Haukness, Glenn Hickey, Shuangjia Lu, Julian K. Lucas, Jean Monlong, Haley J. Abel, et al.from frozen cell pellets (3 × 106 to 7 × 106 cells) using a Maxwell RSC Cultured Cells DNA kit on a Maxwell RSC 48 instrument (Promega). DNA was genotyped at the Children’s Hospital of Philadelphia’s Center for Applied Genomics

(120) The Inhibition and Variability of Two Different RT-qPCR Assays Used for Quantifying SARS-CoV-2 RNA in WastewaterFood and Environmental VirologyMarch 1, 2023George Scott, Nicholas Evens, Jonathan Porter, David I. Walkerinto a Maxwell® RSC PureFood GMO and Authentication Kit cartridge (Promega, US) and extracted on a Maxwell® RSC 48 instrument (Promega, US) following the manufacturer’s guidelines and then eluted into 100 μl elution buffer. EC

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