Maxwell® RSC 48 Instrument from Promega

Product Specs
ItemMaxwell® RSC 48 Instrument
CompanyPromega
Price Supplier Page
Catalog NumberAS8500
QuantityEA

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(141) Comparison of the Virulence of Korean African Swine Fever Isolates from Pig Farms during 2019–2021VirusesNovember 13, 2022Ki-Hyun Cho, Seong-Keun Hong, Min-Kyung Jang, Ji-Hyoung Ryu, Hyun-Jeong Kim, Yu-Ran Lee, In-Soon Roh, Hyun-Joo Sohn, Hae-Eun Kang, Jee-Yong Parkwhole blood and tissue homogenate and swab samples, respectively. The extraction was conducted using a Maxwell® RSC 48 instrument according to the manufacturer’s instructions. OIE TaqMan qPCR was performed using Bio-Rad CFX-96 (Bio-Rad

(142) Enhanced exon skipping and prolonged dystrophin restoration achieved by TfR1-targeted delivery of antisense oligonucleotide using FORCE conjugation in mdx miceNucleic acids researchNovember 11, 2022Desjardins CA, Yao M, Hall J, O'Donnell E, Venkatesan R, Spring S, Wen A, Hsia N, Shen P, Russo R, et al.purchased from ThermoFisher Scientific/Invitrogen, Carlsbad, CA. The Maxwell RSC instrument (Cat. No. AS8500) and Maxwell RSC simplyTissue extraction kit (Cat. No. AS1340) were obtained from Promega, Madison,

(143) Intranasal vaccination induced cross-protective secretory IgA antibodies against SARS-CoV-2 variants with reducing the potential risk of lung eosinophilic immunopathologyVaccineSeptember 1, 2022Takuya Hemmi, Akira Ainai, Takao Hashiguchi, Minoru Tobiume, Takayuki Kanno, Naoko Iwata-Yoshikawa, Shun Iida, Yuko Sato, Sho Miyamoto, Akira Ueno, et al.from 125 µL of nasal or lung wash using ISOGEN-LS (Nippon gene, Toyko, Japan) and purified using a Maxwell RSC 48 Instrument (Promega, Madison, WI) with a Maxwell RSC miRNA Plasma and Serum Kit (Promega). Quantification of subgenomic

(144) Chronic exposure to insecticides impairs honeybee optomotor behaviourFrontiers in Insect ScienceAugust 17, 2022Rachel H. Parkinson, Caroline Fecher, John R. Graygroup. Total RNA was isolated from each sample using the Maxwell® RSC miRNA Tissue Kit (Promega) and the Maxwell® RSC 48 Instrument (Promega) according to the manufacturer’s instructions. RNA concentration was measured using the QuantiFluor® RNA System

(145) Mucociliary Transport Deficiency and Disease Progression in Syrian Hamsters with SARS-CoV-2 InfectionbioRxivJuly 14, 2022Qian Li et al.incubation at room temperature for 10min and at 56°C for additional10min, RNA was extracted utilizing the Maxwell® RSC 48 Instrument (Promega Corporation), and Qrt2-PCR was performed utilizing QuantStudio™ 5 Real-Time PCR instrument

(146) Evaluation of long-term stability of SARS-CoV-2 nucleic acid extracted from human nasopharyngeal samplesJournal of Virological MethodsJuly 1, 2022Priscilla Stela Santana de Oliveira, BÃ¡rbara de Oliveira Silva, RÃ´mulo Pessoa e Silva, LÃlia Vieira Galdino, Vanessa Mylenna FlorÃªncio de Carvalho, Anderson Rodrigues de Almeida, Michelle Melgarejo da Rosa, Moacyr Jesus Barreto de Melo RÃªgo, Maira Galdino da Rocha Pitta, Michelly Cristiny PereiraThen, there was an automated extraction of the RNA using the Maxwell® RSC Viral TNA Kit (AS1330) in the Maxwell RSC 48 Instrument (Promega, Wisconsin, USA). Moreover, the RT-qPCR was carried out in a one single step using GoTaq® Probe 1-Step RT-qPCR System

(147) Emergence of SARS-CoV-2 Spike Mutations during Prolonged Infection in Immunocompromised HostsMicrobiology SpectrumJune 29, 2022Karrie K. K. Ko, Hatairat Yingtaweesittikul, Thuan Tong Tan, Limin Wijaya, Delphine Yanhong Cao, Sui Sin Goh, Nurdyana Binte Abdul Rahman, Kenneth X. L. Chan, Hui Ming Tay, James Heng Chiak Sim, et al.nucleic acid extraction of the chemically inactivated remnant samples was performed on the Promega Maxwell RSC 48 instrument, using the Maxwell RSC viral total nucleic acid purification kit (Promega, USA). cDNA synthesis was

(148) SARS-CoV-2 RT-qPCR testing of pooled saliva samples: A case study of 824 asymptomatic individuals and a questionnaire survey in JapanPLOS ONEMay 12, 2022Junna Oba, Hiroaki Taniguchi, Masae Sato, Masaki Takanashi, Moe Yokemura, Yasunori Sato, Hiroshi Nishiharafollowed by vortexing, heating for 10 min at 56°C, and brief centrifugation. RNA was extracted using the Maxwell RSC 48 Instrument and Viral Total Nucleic Acid Purification Kit (Promega, Madison, WI, USA) and eluted into a total volume

(149) Technical Performance of a 430-Gene Preventative Genomics Assay to Identify Multiple Variant Types Associated with Adult-Onset Monogenic Conditions, Susceptibility Loci, and Pharmacogenetic InsightsJournal of Personalized MedicineApril 21, 2022Ari Silver, Gabriel A. Lazarin, Maxwell Silver, Meghan Miller, Michael Jansen, Christine Wechsberg, Erin Dekanek, Stav Grossfeld, Tim Herpel, Dinura Gunatilake, et al.blood specimens using the Maxwell® RSC Blood DNA Kit (Promega, Madison, Wisconsin) on the automated Maxwell® RSC 48 Instrument (Promega), as well as being sourced from the Coriell Cell Repository. The validation set included 157

(150) DNA Methylation Regulates a Set of Long Non-Coding RNAs Compromising Hepatic Identity during HepatocarcinogenesisCancers (Basel)April 19, 2022Miriam Recalde et al.frozen tissues and cultured cells was isolated using the Maxwell® RSC Cultured Cells DNA Purification Kit with a Maxwell® RSC 48 instrument (Promega, Madison, WI, USA; AS1620). DNA purity and concentration were measured using a NanoDrop spectrophotometer

(151) Molecular Detection of Equine Adenovirus 1 in Nasal Swabs from Horses in the Republic of KoreaVeterinary SciencesApril 13, 2022Sang-Kyu Lee, Jeechan Choi, Jungho Yoon, Jaemin Jung, Joon-Young Park, Jongyoung Park, Yeonjong Kim, Ji-Young Park, Dongsun Parksamples using a Maxwell® RSC Viral Total Nucleic Acid Purification Kit (Promega, Madison, WI, USA) on a Maxwell® RSC 48 Instrument (Promega) according to the manufacturer’s instructions and stored at −80 °C. For EAdV-1 DNA screening,

(152) Soluble CD137 as a dynamic biomarker to monitor agonist CD137 immunotherapiesJournal for ImmunoTherapy of CancerMarch 2, 2022Javier Glez-Vaz, Arantza Azpilikueta, Irene Olivera, Assunta Cirella, Alvaro Teijeira, Maria C Ochoa, Maite Alvarez, IÃ±aki Eguren-Santamaria, Carlos Luri-Rey, Maria E Rodriguez-Ruiz, et al.RT-qPCR Total RNA was extracted from CD8 and PBMCs using a Maxwell RSC simplyRNA tissue kit (Promega) in a Maxwell RSC 48 instrument. M-MLV reverse transcriptase (Invitrogen) and random primers (Invitrogen) were used to obtain cDNA from

(153) SARS-CoV-2 N Gene G29195T Point Mutation May Affect Diagnostic Reverse Transcription-PCR DetectionMicrobiology SpectrumFebruary 23, 2022Karrie K. K. Ko, Nurdyana Binte Abdul Rahman, Shireen Yan Ling Tan, Kenneth X. L. Chan, Sui Sin Goh, James Heng Chiak Sim, Kun Lee Lim, Wan Loo Tan, Kian Sing Chan, Lynette L. E. Oon, et al.laboratory. Total nucleic acid extraction of the chemically inactivated remnant samples was performed on a Maxwell RSC 48 instrument, using the Maxwell RSC viral total nucleic acid purification kit (Promega, USA). The resultant total

(154) A novel Betaretrovirus discovered in cattle with neurological disease and encephalitisRetrovirologyDecember 20, 2021Melanie M. Hierweger, Michel C. Koch, Ronja V. Kauer, Zoltán Bagó, Anna Oevermann, Giuseppe Bertoni, Torsten Seuberlichslaughtered control animals, fresh-frozen medulla oblongata was used. Extraction was performed on the Maxwell® RSC 48 instrument (Promega Corporation, Madison, WI, USA) using the Maxwell® RSC PureFood GMO and Authentication Kit for

(155) Single-cell RNA sequencing reveals distinct tumor microenvironmental patterns in lung adenocarcinomaOncogeneDecember 16, 2021Philip Bischoff, Alexandra Trinks, Benedikt Obermayer, Jan Patrick Pett, Jennifer Wiederspahn, Florian Uhlitz, Xizi Liang, Annika Lehmann, Philipp Jurmeister, Aron Elsner, et al.sections. DNA was isolated using the Maxwell RSC DNA FFPE Kit (Promega, Madison, Wisconsin, USA) on a Maxwell RSC 48 Instrument (Promega) and analyzed using the nNGM panel v1 (ThermoFisher, Waltham, Massachusetts, USA), an Ion 530

(156) Population genomics and haplotype analysis in spelt and bread wheat identifies a gene regulating glume colorCommunications BiologyDecember 1, 2021Michael Abrouk et al.were collected for RNA isolation. RNA extraction was done using the Maxwell® RSC Plant RNA kit using a Maxwell® RSC 48 instrument (Promega). In all, 1 µg of RNA was used for the first-strand complementary DNA (cDNA) synthesis using

(157) Molecular Prevalence of Equine Parvovirus-Hepatitis in the Sera of Clinically Healthy Horses in South KoreaVeterinary SciencesNovember 19, 2021Sang-Kyu Lee, Dongsun Park, Inhyung Leeusing the Maxwell® RSC Viral Total Nucleic Acid Purification Kit (Promega, Madison, WI, USA) on the Maxwell® RSC 48 Instrument (Promega) according to the manufacturer’s instructions and stored at –70 °C until use. For EqPV-H DNA

(158) Enhanced rapid commercial DNA extraction kit for the molecular detection of severe acute respiratory syndrome coronavirus 2: Easy adaptation to current protocolsRevista da Sociedade Brasileira de Medicina TropicalNovember 12, 2021Rômulo Pessoa-e-Silva, Priscilla Stela Santana de de Oliveira, Sayonara Maria Calado Gonçalves, Klarissa Miranda Guarines, Lidiane Vasconcelos do Nascimento Carvalho, Maria Andreza Bezerra Correia, Michelle Melgarejo da da Rosa, Moacyr Jesus Barreto de Melo Rêgo, Maira Galdino da Rocha Pitta, Michelly Cristiny Pereirawere the Maxwell® RSC Viral Total Nucleic Acid kit (AS1330), for use in robotic extraction with the Maxwell RSC 48 Instrument (Promega, Madison, WI, USA) using magnetic beads (sample volume, 200 µL; elution volume, 50 µL of RNase-free

(159) SARS-CoV-2 Infection in One Cat and Three Dogs Living in COVID-19-Positive Households in Madrid, SpainFrontiers in Veterinary ScienceNovember 10, 2021Guadalupe Miró, Javier Regidor-Cerrillo, Rocio Checa, Carlos Diezma-Díaz, Ana Montoya, Jesús García-Cantalejo, Pedro Botías, Javier Arroyo, Luis-Miguel Ortega-Moramedium using the Maxwell® RSC Viral Total Nucleic Acid Purification Kit for automated extraction in a Maxwell® RSC 48 Instrument (Promega, Madrid, Spain). Viral RNA detection was performed using the multiplex TaqPath™ COVID-19 CE-IVD

(160) Tongue nodules in an atypical canine leishmaniasis in BrazilJournal of Veterinary Medical ScienceOctober 5, 2021João José da COSTA NETO, Camila Neves MARTINS, Karen Santos MARÇO, Beatriz Furlan PAZ, Guilherme Paz MONTEIRO, Roberta Torres de de MELO, Francisco Cláudio Dantas MOTA, Aracelle Elisane ALVESqPCR was performed to confirm the molecular identity of the Leishmania spp. The DNA was extracted (Maxwell® RSC 48 Instrument) (Promega, São Paulo, Brazil) from a sample collected from the animal’s bone marrow and tested for conserved

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