Maxwell® RSC 48 Instrument from Promega

Product Specs
ItemMaxwell® RSC 48 Instrument
CompanyPromega
Price Supplier Page
Catalog NumberAS8500
QuantityEA

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(61) Mutant KRAS and GATA6 Stratify Survival in Patients Treated with Chemotherapy for Pancreatic Adenocarcinoma: A Prospective Cohort StudyCancers (Basel)March 5, 2025Jung Won Chun, Dong-Eun Lee, Nayoung Han, SooBeen Heo, Hyeji Kim, Mi Rim Lee, Hyeong Min Park, Sung-Sik Han, Sang-Jae Park, Tae Hyun Kim, et al.were performed from FFPE samples using a Maxwell RSC RNA FFPE kit (Promega, Madison, WI, USA) on a Maxwell RSC 48 Instrument (Promega), according to the manufacturer’s protocol. The RNA was eluted in 40 µL of nuclease-free water.

(62) Intronic SYNE1 Gene Novel Variant Associated with Myocardial Infarction in Young People with a Family History of Premature Atherosclerosis: A Case–Control Study in the Polish Population †Int J Mol SciMarch 3, 2025Michał Ambroziak, Jakub Franke, Anna Wójcicka, Monika Kolanowska, Tomasz Jaxa-Chamiec, Andrzej BudajGenotyping Total DNA was extracted from whole peripheral blood samples. The extraction was performed on a Maxwell® RSC 48 Instrument (Promega, Madison, WI, USA) using the Maxwell® RSC Blood DNA Kit, according to the protocol. The targeted

(63) Machine learning-optimized targeted detection of alternative splicingNucleic Acids ResearchJanuary 24, 2025Kevin Yang, Nathaniel Islas, San Jewell, Di Wu, Anupama Jha, Caleb M Radens, Jeffrey A Pleiss, Kristen W Lynch, Yoseph Barash, Peter S Choiindependent days. RNA isolation and processing Total RNA was purified from cell pellets with the Maxwell RSC 48 instrument using the Maxwell RSC simplyRNA Cells Kit (#AS1340, Promega). Poly-A selection was subsequently performed

(64) Development and validation of PCR marker array for molecular selection towards spring, vernalization-independent and winter, vernalization-responsive ecotypes of white lupin (Lupinus albus L.)Sci RepJanuary 21, 2025Anna Surma, Michał Książkiewicz, Wojciech Bielski, Bartosz Kozak, Renata Galek, Sandra Rychel-BielskaMaxwell® RSC PureFood GMO and Authentication Kit (Promega, Mannheim, Germany)131 and automated isolation station Maxwell® RSC 48 Instrument (Promega). No changes to the standard protocol were made. DNA concentration and quality were estimated

(65) FORCE platform overcomes barriers of oligonucleotide delivery to muscle and corrects myotonic dystrophy features in preclinical modelsCommun Med (Lond)January 18, 2025Weeden T, Picariello T, Quinn B, Spring S, Shen PY, Qiu Q, Vieira BF, Schlaefke L, Russo RJ, Chang YA, et al.controls were subjected to total RNA extraction with the Maxwell RSC simplyRNA Tissue Kit using the Maxwell RSC 48 instrument (Promega) in accordance with the manufacturer’s protocol. Purified total RNA was reverse-transcribed

(66) Unraveling Effects of miRNAs Associated with APR Leaf Rust Resistance Genes in Hybrid Forms of Common Wheat (Triticum aestivum L.)Int J Mol SciJanuary 14, 2025Julia Spychała, Aleksandra Noweiska, Agnieszka Tomkowiak, Roksana Bobrowska, Katarzyna Szewczyk, Michał Tomasz Kwiatekusing the Maxwell RSC Plant RNA Isolation Kit (Promega, Madison, WI, USA) harnessing automated station Maxwell® RSC 48 Instrument (Promega). During RNA isolation, samples were treated with DNAase according to the manufacturer’s recommendations.

(67) Oral administration of Lactobacillus casei DG® after ileostomy closure in restorative proctocolectomy: a randomized placebo-controlled trial (microbiota and immune microenvironment in pouchitis -MEP1)Gut MicrobesDecember 31, 2024Imerio Angriman, Melania Scarpa, Edoardo Savarino, Ilaria Patuzzi, Alessandra Rigo, Andromachi Kotsafti, Astghik Stepanyan, Elisa Sciuto, Francesco Celotto, Silvia Negro, et al.Maxwell® RSC Fecal Microbiome DNA Kit following the manufacturer’s instructions (Promega, Madison, Wisconsin) on a Maxwell® RSC 48 Instrument (Promega). The sequencing protocol was performed at BMR Genomics Srl. Briefly: V3–V4 regions of 16S

(68) Performance evaluation of nine reference centers and comparison of DNA extraction protocols for effective surveillance of Leishmania-infected Phlebotomine sand flies: Basis for technical recommendationsPLOS Neglected Tropical DiseasesDecember 23, 2024Jorian Prudhomme, Aymeric Delabarre, Bulent Alten, Umut Berberoglu, Eduardo Berriatua, Gioia Bongiorno, José Manuel Cristovao, Maya Davidovich-Cohen, Trentina Di Muccio, Ozge Erisoz Kasap, et al.RSC Viral TNA (Promega, Southampton, England) and RSC Blood DNA (Promega, Southampton, England) using Maxwell RSC 48 instrument (Promega). Amplifications were realized using a QuantStudio 5 Real-Time PCR System (Applied Biosystems,

(69) Unravelling turbot (Scophthalmus maximus) resistance to Aeromonas salmonicida: transcriptomic insights from two full-sibling families with divergent susceptibilityFrontiers in ImmunologyDecember 6, 2024Patricia Pereiro, Ricardo Tur, Miguel García, Antonio Figueras, Beatriz Novoavalidation RNA was isolated with the Maxwell RSC simplyRNA Tissue Kit (Promega) with an automated Maxwell RSC 48 Instrument following the guidelines outlined by the manufacturer. The quantity of RNA was measured with a NanoDrop

(70) Oxidative Stress and Inflammation-Related mRNAs Are Elevated in Serum of a Finnish Wet AMD CohortInvestigative Ophthalmology & Visual ScienceNovember 15, 2024Mikko Liukkonen, Hanna Heloterä, Leea Siintamo, Bishwa Ghimire, Pirkko Mattila, Niko Kivinen, Joanna Kostanek, Cezary Watala, Maria Hytti, Juha Hyttinen, et al.of Life Science and Biocenter Finland. Total RNA was extracted from the serum samples (1 mL) using Maxwell RSC 48 Instrument and Maxwell RSC miRNA Plasma and Serum Kit (Promega, Madison, WI) according to the manufacturer's instructions,

(71) Effect of nicotinamide riboside on airway inflammation in COPD: a randomized, placebo-controlled trialNature AgingNovember 15, 2024Kristoffer L. Norheim, Michael Ben Ezra, Indra Heckenbach, Louise Munkholm Andreasson, Lise Lotte Eriksen, Nanna Dyhre-Petersen, Mads Vargas Damgaard, Magnus Berglind, Luca Pricolo, Dayle Sampson, et al.AutoAnnotate. DNA methylation PBMCs were shipped on dry ice and analyzed by Tempus Labs. DNA was isolated on a Maxwell RSC 48 instrument using the Maxwell RSC Blood DNA Kit (Promega, AS1400) according to the manufacturer’s protocol. In brief,

(72) Comprehensive in silico CpG methylation analysis in hepatocellular carcinoma identifies tissue- and tumor-type specific marks disconnected from gene expressionJournal of Physiology and BiochemistryNovember 1, 2024Idoia Bilbao, Miriam Recalde, Fabrice Daian, José Maria Herranz, María Elizalde, Mercedes Iñarrairaegui, Matteo Canale, Maite G. Fernández-Barrena, Andrea Casadei-Gardini, Bruno Sangro, et al.from frozen tissues was isolated using the Maxwell® RSC Cultured Cells DNA Purification Kit with a Maxwell® RSC 48 instrument (Promega, Madison, WI, USA; AS1620). DNA purity and concentration were measured using a NanoDrop spectrophotometer

(73) Rethinking asexuality: the enigmatic case of functional sexual genes in Lepraria (Stereocaulaceae)BMC GenomicsOctober 26, 2024Meredith M. Doellman, Yukun Sun, Alejandrina Barcenas-Peña, H. Thorsten Lumbsch, Felix Grewethe Qiagen TissueLyser II (Qiagen, Hilden, Germany). The extraction was completed according to the Maxwell® RSC 48 Instrument (Promega, Madison, WI) protocol. Stranded poly(a) selected libraries were then prepared with the NEXTFLEX®

(74) Impaired neutrophil-mediated cell death drives Ewing’s Sarcoma in the background of Down syndromeFrontiers in OncologyOctober 3, 2024Serena Peirone, Elisa Tirtei, Anna Campello, Caterina Parlato, Simonetta Guarrera, Katia Mareschi, Elena Marini, Sebastian Dorin Asaftei, Luca Bertero, Mauro Papotti, et al.μm-thick FFPE sections (3–6 sections per sample) using Maxwell® RSC DNA FFPE Kit (Promega Corporation) on Maxwell® RSC 48 Instrument (Promega Corporation), following the manufacturer’s protocol. Peripheral blood was used as a matching

(75) A preliminary indication that HLA-A*03:01 may be associated with visceral leishmaniasis development in people living with HIV in EthiopiaPLOS Neglected Tropical DiseasesSeptember 30, 2024Nicky de Vrij, Romi Vandoren, Kadrie Ramadan, Anke Van Hul, Ann Ceulemans, Mekibib Kassa, Roma Melkamu, Arega Yeshanew, Tadfe Bogale, Hailemariam Beyene, et al.extraction and quantification DNA was extracted from the PBMC samples of all participants using the Maxwell RSC 48 instrument (Promega, Wisconsin, U.S.A) using the Maxwell RSC Whole Blood DNA kit (Promega, Wisconsin, U.S.A.) according

(76) Comparison of commercial DNA kits for allergen detection of celery in food matricesHeliyonSeptember 15, 2024Marleen M. Voorhuijzen-Harink, Bas J. Fronen, Linda Willemsen, Andries Koops, Elise F. Hoek-van den Hil, Nathalie G.E. Smitsof the supernatant was transferred into a Maxwell cartridge, and samples were further purified in a Maxwell® RSC 48 instrument (Promega, Wisconsin, USA) using the PureFood GMO and authentication method. DNA was eluted in 100 μL

(77) A comparative field efficacy trial of three treatment programs against endo- and ectoparasites in naturally infected dogsFrontiers in Veterinary ScienceSeptember 5, 2024Cameron Raw, Rebecca J. Traub, Anke Wiethoelter(Catalog no. AS1600, Promega Corporation, Madison, USA) with the Maxwell® RSC 48 Instrument (Catalog no. AS8500, Promega Corporation, Madison, USA) using a modified method as described by Massetti et al. (26). Extracted

(78) Selection for high and low antibody responses to sheep red blood cells influences cytokine and chemokine expression in chicken peripheral blood leukocytes and splenic tissuePoultry ScienceSeptember 1, 2024Christina L. Swaggerty, Paul B. Siegel, Christa F. Honaker, Michael H. Kogut, Robin C. Anderson, Christopher M. Ashwell, Robert L. Taylorthen loaded into the Maxwell cartridge for RNA isolation. All RNA extractions were carried out on the Maxwell® RSC 48 instrument (Promega Corp.). Upon completion, the purified RNA samples were stored at -80°C. Quantitative Real-Time RT-PCR

(79) Lipid-encapsulated mRNA encoding an extended serum half-life interleukin-22 ameliorates metabolic disease in miceMolecular metabolismAugust 1, 2024Canali S, Fischer AW, Nguyen M, Anderson K, Wu L, Graham AR, Hsiao CJ, Bankar C, Dussault N, Ritchie V, et al.Maxwell RSC simplyRNA Tissue Kit (Promega, cat no. AS1340) and automated Maxwell RSC 48 instrument (Promega, cat no. AS8500). Total liver RNA was isolated using QIAshredder columns (Qiagen, cat no. 79656) and RNeasy mini purification

(80) A GWAS study highlights significant associations between a series of indels in a FLOWERING LOCUS T gene promoter and flowering time in white lupin (Lupinus albus L.)BMC Plant BiologyJuly 29, 2024Sandra Rychel-Bielska, Wojciech Bielski, Anna Surma, Paolo Annicchiarico, Jolanta Belter, Bartosz Kozak, Renata Galek, Nathalie Harzic, Michał KsiążkiewiczMaxwell® RSC PureFood GMO and Authentication Kit (Promega, Mannheim, Germany) harnessing automated station Maxwell® RSC 48 Instrument (Promega) with standard protocol, yielding on average 100 ± 29 µg DNA per sample. DNA concentration

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