DAPI from Cell Signaling Technology

Product Specs
ItemDAPI
CompanyCell Signaling Technology
Price $74.00 Inquire
Catalog Number4083S
Quantity1 mg
Research AreaRelated Products
ApplicationsIF-IC, FC-FP

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(161) FAM222A, Part of the BET-Regulated Basal Endothelial Transcriptome, Is a Novel Determinant of Endothelial Biology and Angiogenesis—Brief ReportArteriosclerosis, Thrombosis, and Vascular BiologyJanuary 1, 2024Aspasia Tzani, Stefan Haemmig, Henry S. Cheng, Daniel Pérez-Cremades, Marina Augusto Augusto Heuschkel, Anurag Jamaiyar, Sasha A. Singh, Masanori Aikawa, Paul B. Yu, Tianxi Wang, et al.Thermo Fisher). Cell nuclei were stained with 4′,6-diamidino- 2-phenylindole (DAPI, 0.5 ug/ml, Cat#: 4083, Cell Signaling). Retinas were imaged using a Zeiss AxioObserver.Z1 microscope and AxioCam MRm camera.

(162) Identification of a novel aromatic-turmerone analog that activates chaperone-mediated autophagy through the persistent activation of p38Frontiers in cell and developmental biologyJanuary 1, 2024Motomura K, Ueda E, Boateng A, Sugiura M, Kadoyama K, Hitora-Imamura N, Kurauchi Y, Katsuki H, Seki T.from Biotex Laboratories (München, Germany). 4′, 6-Diamidino-2-phenylindole, dihydrochloride (DAPI, #4083) was purchased from Cell Signaling Technology (Danvers, MA, United States). 2.2 Synthesis of ar-turmerone

(163) Wilms' tumor 1 (WT1) antigen is overexpressed in Kaposi Sarcoma and is regulated by KSHV vFLIPPLoS pathogensJanuary 1, 2024Morales AE, Gumenick R, Genovese CM, Jang YY, Ouedraogo A, Ibáñez de Garayo M, Pannellini T, Patel S, Bott ME, Alvarez J, et al.apoptosis detection, cells were stained using Annexin V (Biolegend 640920) and DAPI (Cell Signaling Technology, 4083S). Statistics Statistical analyses were performed using GraphPad Prism 9.4.1 (GraphPad Software) and

(164) Distinguished biomimetic dECM system facilitates early detection of metastatic breast cancer cellsBioengineering & Translational MedicineJanuary 1, 2024Bowen Weng, Mei Li, Weilai Zhu, Jing Peng, Xufeng Mao, Yanan Zheng, Chi Zhang, Senhao Pan, Haijiao Mao, Jiyuan Zhaostaining, the samples were fixed with 4% formaldehyde for 15 min and rinsed with PBS. DAPI solution (#4083, Cell Signaling Technology) at the concentration of 1 μg/mL was added, and the samples were incubated

(165) Chemokine‑like receptor 1‑positive cells are present in the odontoblast layer in tooth tissue in rats and humansExperimental and Therapeutic MedicineDecember 27, 2023Daisuke Yokokawa, Naoki Umemura, Yuka Miyamoto, Nobuo Kondoh, Satoshi KawanoCell Signaling Technology, Inc.) were added, incubated in the dark for 1 h and counterstained with DAPI (cat. no. 4083, 1:2,000; Cell Signaling Technology, Inc.) for 5 min in the dark at room temperature. After dehydration

(166) AMPK activation induces immunogenic cell death in AMLBlood advancesDecember 26, 2023Mondesir J, Ghisi M, Poillet L, Bossong RA, Kepp O, Kroemer G, Sarry JE, Tamburini J, Lane AA.(ThermoFisher Scientific, no. BDB556463); 4′,6-diamidino-2-phenylindole (Cell Signaling Technology, no. 4083S); Zombie Green (BioLegend, no. 423111); Zombie Aqua (BioLegend, no. 423101); anti-CALR antibody, ER

(167) Differential LRRK2 Signalling and Gene Expression in WT-LRRK2 and G2019S-LRRK2 Mouse Microglia Treated with Zymosan and MLi2CellsDecember 26, 2023Iqra Nazish, Adamantios Mamais, Anna Mallach, Conceicao Bettencourt, Alice Kaganovich, Thomas Warner, John Hardy, Patrick A. Lewis, Jennifer Pocock, Mark R. Cookson, et al.Clara, USA,#GA524), and anti- 4′,6-diamidino-2-phenylindole (DAPI) (Cell Signaling, Danfoss, MA, USA, #4083). The primary microglial yield was approximately 5 × 105 cells/flask with minimal astrocyte contamination.

(168) Natural Fatty Acid Guards against Brain Endothelial Cell Death and Microvascular Pathology following Ischemic Insult in the Presence of Acute HyperglycemiaBiomedicinesDecember 18, 2023Zaib Ali Shaheryar, Mahtab Ahmad Khan, Huma Hameed, Muhammad Naveed Mushtaq, Sajjad Muhammad, Gamal A. Shazly, Ali Irfan, Yousef A. Bin JardanMA, USA; and Goat anti-rabbit (1:1000), Proteintech, respectively. To stain the nuclei, we used DAPI #4083; 1:500; Cell Signaling Technology (Cambridge, UK). For 4-HNE staining, we incubated brain slices with

(169) Integration of xeno-free single-cell cloning in CRISPR-mediated DNA editing of human iPSCs improves homogeneity and methodological efficiency of cellular disease modelingStem Cell ReportsDecember 12, 2023Atefeh Namipashaki, Kealan Pugsley, Xiaodong Liu, Kirra Abrehart, Sue Mei Lim, Guizhi Sun, Marco J. Herold, Jose M. Polo, Mark A. Bellgrove, Ziarih Hawipenicillin-streptomycin (Gibco, cat. no. 15140122), 5 μL of DAPI (Cell Signaling Technology, cat. no. 4083S), and 2 μL of 0.5 M EDTA (Invitrogen, cat. no. 15575020) and passed through a Falcon 5 mL tube with

(170) KCNQ potassium channels modulate Wnt activity in gastro-oesophageal adenocarcinomasLife science allianceDecember 1, 2023Shorthouse D, Zhuang L, Rahrmann EP, Kosmidou C, Wickham Rahrmann K, Hall M, Greenwood BM, Devonshire G, Gilbertson RJ, Fitzgerald RC, et al.for non-specific staining of each antibody. Sections were then counterstained using DAPI (1:10,000, 4083; Cell Signaling) and mounted using ProLong Gold Antifade Mountant (P36930; Thermo Fisher Scientific).

(171) Sorting nexin 3 exacerbates doxorubicin-induced cardiomyopathy via regulation of TFRC-dependent ferroptosisActa Pharmaceutica Sinica BDecember 1, 2023Wenjing Yu, Yuehuai Hu, Zhiping Liu, Kaiteng Guo, Dinghu Ma, Mingxia Peng, Yuemei Wang, Jing Zhang, Xiaolei Zhang, Panxia Wang, et al.with fluorescent secondary antibodies (4412S, 8890S; CST) for 1 h at room temperature. Finally, DAPI (4083; CST) was used to label the nuclei. Fluorescence was detected below the FV3000 fluorescent microscope

(172) Epigenetically Downregulated Breast Cancer Gene 2 through Acetyltransferase Lysine Acetyltransferase 2B Increases the Sensitivity of Colorectal Cancer to OlaparibCancers (Basel)November 25, 2023Siche Chen, Heike Allgayerh at room temperature in the dark, followed by setting with DAPI (Cell Signaling, Danvers, MA, USA, 4083S) for 20 min. Coverslips were washed and mounted in an anti-fade mounting medium. The fluorescence images

(173) Activation of KrasG12D in Subset of Alveolar Type II Cells Enhances Cellular Plasticity in Lung AdenocarcinomaCancer Res CommunNovember 24, 2023Chaudhary P, Xu X, Wang G, Hoj JP, Rampersad RR, Asselin-Labat ML, Ting S, Kim W, Tamayo P, Pendergast AM, et al.Fluor-coupled secondary antibodies (Invitrogen) were used at 1:500 dilution. DAPI (Cell Signaling Technology, 4083) was used to stain nuclei at a concentration of 0.1 mmol/L. Images were captured on a Leica Sp2 laser

(174) 5′-Methylthioadenosine strongly suppresses RANKL-induced osteoclast differentiation and function via inhibition of RANK-NFATc1 signalling pathwaysHeliyonNovember 18, 2023Purithat Rattajak, Aratee Aroonkesorn, Carl Smythe, Rapepun Wititsuwannakul, Thanawat PitakpornpreechaSanta Cruz Biotechnology, Dallas, TX, USA) was added and incubated for 3 h and counterstained with DAPI (1 μg/mL) (No. 4083, Cell Signaling Technology, Danvers, MA) for 5 min. Lastly, a mounting medium was carefully added to

(175) Regulation of Drp1 and enhancement of mitochondrial fission by the deubiquitinating enzyme PSMD14 facilitates the proliferation of bladder cancer cellsOncology ReportsNovember 16, 2023Wei Song, Zhuo Li, Ming Xia, Wei XiaoCell Signaling Technology, Inc.), or anti-TOM20 (cat. no. 42406S; 1:200; Cell Signaling Technology, Inc.). DAPI (cat. no. 4083S; Cell Signaling Technology, Inc. 1:200) was used to counterstain the nuclei. The secondary antibodies

(176) Canonical Wnt and TGF-β/BMP signaling enhance melanocyte regeneration but suppress invasiveness, migration, and proliferation of melanoma cellsFrontiers in Cell and Developmental BiologyNovember 13, 2023Esra Katkat, Yeliz Demirci, Guillaume Heger, Doga Karagulle, Irene Papatheodorou, Alvis Brazma, Gunes OzhanJackson Immunoresearch Laboratories, PA, United States). Nuclear staining was carried out using 4′,6-diamidino-2-phenylindole (DAPI; 4083S, Cell Signaling Technology, MA, United States). Larvae were imaged using fluorescence confocal microscopy. Confocal

(177) NUAK1 promotes tumor metastasis through upregulating slug transcription in esophageal squamous cell carcinomaCancer Cell InternationalNovember 2, 2023Huiru Yang, Zhen Wei, Yifan Song, Kexin Du, Nannan Yin, Hong Lu, Bingbing Li, Lili Hou, Panfei Xing, Liang Chen, et al.room temperature. After washing thrice with PBS, the cells were by incubation for 5 min with DAPI (#4083, 0.5 µg/mL, CST) to label the nuclei. Images were captured using a confocal scanning microscope (Leica

(178) Comparative membrane lipidomics of hepatocellular carcinoma cells reveals diacylglycerol and ceramide as key regulators of Wnt/β‐catenin signaling and tumor growthMolecular OncologyNovember 1, 2023Yagmur Azbazdar, Yeliz Demirci, Guillaume Heger, Dogac Ipekgil, Mustafa Karabicici, Gunes Ozhan5 min each, their nuclei were counterstained with 4′,6‐diamidino‐2‐phenylindole (DAPI, 0.5 μg·mL−1, 4083S, Cell Signaling Technology, MA, USA) and imaged by using an LSM 880 laser scanning confocal microscope

(179) Addition of exogenous diacylglycerol enhances Wnt/β-catenin signaling through stimulation of macropinocytosisiScienceOctober 20, 2023Azbazdar Y, Tejeda-Munoz N, Monka JC, Dayrit A, Binder G, Ozhan G, De Robertis EM.times and were mounted using mounting medium including 4′,6-diamidino-2-phenylindole (DAPI, 0.5 ug/ml, 4083S, Cell Signaling Technology, MA, USA). Immunostainings were imaged with a Carl Zeiss Axio Observer Z1

(180) Plasma 8-OHdG act as a biomarker for steroid-induced osteonecrosis of the femoral headBMC Musculoskeletal DisordersOctober 12, 2023Peng Peng, Mincong He, Weihua Fang, Mengqi Lai, Fangjun Xiao, Wei He, Huan Xiao, Qiushi Wei37°C for 2 h. The slices were stained with DAPI nuclear staining (Cell Signaling Technology, cat. #4083) for 1 min. Use the same antibody as for Immunoshistochemistry. Measurement of 8-OHdG concentrations Plasma

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