DAPI from Cell Signaling Technology

Product Specs
ItemDAPI
CompanyCell Signaling Technology
Price $74.00 Inquire
Catalog Number4083S
Quantity1 mg
Research AreaRelated Products
ApplicationsIF-IC, FC-FP

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(121) c-Rel drives pancreatic cancer metastasis through Fibronectin-Integrin signaling-induced isolation stress resistance and EMT activationbioRxiv : the preprint server for biologyFebruary 1, 2025Bakırdöğen D, Görgülü K, Xin J, Alcalá S, Ruiz-Cañas L, Frank K, Wu N, Diakopoulos KN, Dai C, Öztürk H, et al.PBS with 1:5000 Zombie-Red (BioLegend 423110), or 300μL FC buffer with 1:5000 DAPI (Cell Signaling, 4083S), depending on the panel. Before analyses, suspensions are passed through a 35μm strainer (Corning 352235).

(122) Bacteria in hypertrophic scars promote scar formation through HSBP1-mediated autophagyWound Repair RegenJanuary 17, 2025Bo Yuan, Jiarong Yu, Jiaoyun Dong, Zhigang Mao, Xiqiao Wangincubated with 4′,6‐diamidino‐2‐phenylindole (DAPI, 1:1000, Cell Signalling Technology, catalogue number 4083) for an appropriate duration. Following three additional washes, the slides were mounted and observed

(123) Innovative treatment of age-related hearing loss using MSCs and EVs with ApelinCell biology and toxicologyJanuary 17, 2025Xu S, Liu D, Zhang F, Tian Y.thorough wash with PBS before being affixed using an anti-fade mounting compound infused with DAPI (4083, CST). Subsequent observations and image capture were performed utilizing a laser scanning confocal

(124) AAVR Expression is Essential for AAV Vector Transduction in Sensory Hair CellsAdvanced ScienceJanuary 7, 2025Fan Wu, Guisheng Chen, Rui Hu, Peiwen Liu, Jintao Lou, Wenji Zhao, Zuhong He, Suhua Sha, Yiqing Zheng1:200 at 4 °C overnight. Nuclei were counterstained with DAPI (1:1000, Cell Signaling Technology, #4083) at room temperature for 10 min. Immunolabeled images were taken with a Zeiss LSM 880. Immuno‐Colocalization

(125) Increased in vivo transduction of AAV-9 cargo in Alport podocytesWellcome open researchJanuary 1, 2025Williams E, Fresquet M, Bolas G, Kaseda S, Goncalves KA, Steinsapir A, Adamson A, Sherwood DR, Lennon R.Abcam Cat# ab150130; RRID:AB_2927775) for 1 hour at room temperature. DAPI (1:2000, Cell Signaling 4083) was used for nuclear staining. Images were collected on a Zeiss Axioimager.D2 upright microscope using

(126) Bioengineered miR-7-5p modulates non–small cell lung cancer cell metabolism to improve therapyMolecular PharmacologyJanuary 1, 2025Gavin M. Traber, Mei-Juan Tu, Su Guan, Neelu Batra, Ai-Ming YuCat# A22286) and with 4ʹ,6-diamidino-2-phenylindole (DAPI; 1 μg/mL; Cell signaling Technology Cat# 4083S) nuclear stain prior to mounting with VECTASHIELD Antifade (20 μL/chamber slide well; Fisher Scientific

(127) In vivo hyperphosphorylation of tau is associated with synaptic loss and behavioral abnormalities in the absence of tau seedsNature NeuroscienceDecember 24, 2024Naoto Watamura, Martha S. Foiani, Sumi Bez, Mathieu Bourdenx, Alessia Santambrogio, Claire Frodsham, Elena Camporesi, Gunnar Brinkmalm, Henrik Zetterberg, Saisha Patel, et al.Finally, sections were stained with 4,6-diamidino-2-phenylindole (Cell Signaling Technology, cat. no. 4083S) diluted in TNB buffer before mounting with PermaFluor (Thermo Fisher Scientific, cat. no. TA-030-FM).

(128) BPDCN MYB fusions regulate cell cycle genes, impair differentiation, and induce myeloid-dendritic cell leukemiaJCI insightDecember 20, 2024Booth CAG, Bouyssou JM, Togami K, Armand O, Rivas HG, Yan K, Rice S, Cheng S, Lachtara EM, Bourquin JP, et al.washed twice in PBS + 2% FBS and then resuspended in PBS + 1 μg/mL DAPI (Cell Signaling Technology, 4083) + 0.1 μg/mL RNase A (Thermo Fisher Scientific, EN0531) and incubated for 1 hour at 22°C before flow cytometry analysis

(129) Protocol for isolating CD163+ Kupffer cells from human liver resectionsSTAR ProtocolsDecember 20, 2024Armando Andres Roca Suarez, Marie-Laure Plissonnier, Maud Michelet, Anaëlle Dubois, Sarah Heintz, Maria Saez-Palma, Marion Delphin, Isabelle Bordes, Jennifer Molle, Audrey Diederichs, et al.serum,Cytiva,SH30066.03 OptiPrep density gradient medium,BioVision,M1248-250 DAPI,Cell Signaling Technology,4083 Paraformaldehyde (PFA) 16% solution,Electron Microscopy Sciences,15710 Dako fluorescent mounting medium,Dako,S3023 Selgantolimod

(130) Optogenetically Induced Microtubule Acetylation Unveils the Molecular Dynamics of Actin-Microtubule Crosstalk in Directed Cell MigrationbioRxivDecember 8, 2024Abhijit Deb Deb Roy, Cristian Saez Saez Gonzalez, Farid Shahid, Eesha Yadav, Takanari Inoueincubated with secondary antibody, Phalloidin (ThermoFisher A22286) and DAPI (Cell Signaling Technology, 4083S). After that, they were washed three times in PBS and the images were captured by microscopy. For HeLa cells

(131) An In Vitro Oxidative Stress Model of the Human Inner Ear Using Human-Induced Pluripotent Stem Cell-Derived Otic Progenitor CellsAntioxidants (Basel)November 16, 2024Minjin Jeong, Sho Kurihara, Konstantina M. Stankovic1:1000). The nuclei were stained with 0.1 μg/mL DAPI (Cell Signaling Technology, Danvers, MA, USA, #4083) and Vectashield (Vector Laboratories, Burlingame, CA, USA, # H1000) was used to mount the samples.

(132) Inhibition of the Notch signal transducer CSL by Pkc53E-mediated phosphorylation to fend off parasitic immune challenge in DrosophilaeLifeNovember 6, 2024Deichsel S, Frankenreiter L, Fechner J, Gahr BM, Zimmermann M, Mastel H, Preis I, Preiss A, Nagel AC.or kit,Pap-pen,Kisker Biotech,Cat# MKP-1, "Chemical compound, drug",DAPI,Cell Signaling Techn.,Cat# 4083,(1 µg/ml) "Chemical compound, drug",DNase I,New England Biolabs GmbH,Cat# M0303,(2 U/µl) "Chemical

(133) Baicalein suppresses inflammation and attenuates acute lung injury by inhibiting glycolysis via HIF‑1α signalingMolecular Medicine ReportsNovember 5, 2024Zhongyou Liu, Xiaona Zheng, Ning Li, Zongyao WangCell Signaling Technology, Inc.) was added and incubated at 37°C for 1 h. The sections were incubated with DAPI (cat. no. 4083; Cell Signaling Technology, Inc.) in the dark at room temperature for 5 min for nuclear counterstaining.

(134) Lentiviral vector gene therapy and CFTR modulators show comparable effectiveness in cystic fibrosis rat airway modelsGene therapyNovember 1, 2024McCarron A, Ling KM, Montgomery ST, Martinovich KM, Cmielewski P, Rout-Pitt N, Kicic A, Parsons D, Donnelley M.antibody dilution buffer. The secondary antibody was washed and a DAPI nuclear counterstain was applied (4083S, Cell Signalling Technology). Slides were washed and mounted with ProLong Diamond Antifade mountant

(135) Dietary pro-oxidant therapy by a vitamin K precursor targets PI 3-kinase VPS34 functionScienceOctober 25, 2024Manojit Mosur Swamynathan, Shan Kuang, Kaitlin E. Watrud, Mary R. Doherty, Charlotte Gineste, Grinu Mathew, Grace Q. Gong, Hilary Cox, Eileen Cheng, David Reiss, et al.200 ml of 106 mM MgCl2, 10 ml of 500 mM EDTA at pH 8.0, and 10 mg of DAPI (Cell Signaling Technology, 4083S). Nuclei were prepared by gently centrifuging the cell lysis solution at 1,000 rpm for 5 minutes to

(136) Lysosomal Ca(2+) release-facilitated TFEB nuclear translocation alleviates ischemic brain injury by attenuating autophagic/lysosomal dysfunction in neuronsScientific reportsOctober 22, 2024Lei Q, Chen X, Xiong Y, Li S, Wang J, He H, Deng Y.were labeled for 2 h in the dark and then counterstained with DPAI (1:1000; Cell Signaling Technology, 4083, Danvers, MA, USA). Finally, the reactions were observed and photographed with a fluorescence microscope

(137) Leucine restriction ameliorates Fusobacterium nucleatum-driven malignant progression and radioresistance in nasopharyngeal carcinomaCell Reports MedicineOctober 15, 2024Songhe Guo, Shan Xing, ZhenYu Wu, Fangfang Chen, Xiaoyun Pan, Qifan Li, Wanli Liu, Ge ZhangSR305376 Neomycin,MedChemExpress,Cat# HY-150520; ACS: 1404-04-2 DAPI,Cell Signaling Technology,Cat# 4083 Ci [3 H]-L-leucine,Perkin Elmer,Cat# NET116600 XF 1.0 M Glucose Solution,Agilent Technology,Cat# 103577-100 Seahorse

(138) NPM1 inhibits tumoral antigen presentation to promote immune evasion and tumor progressionJournal of Hematology & OncologyOctober 14, 2024Xin Wang, Yangyang Chai, Yuan Quan, Jiaming Wang, Jiaying Song, Wenkai Zhou, Xiaoqing Xu, Henan Xu, Bingjing Wang, Xuetao CaoFluo 594 anti-rabbit secondary antibody (Cat#A-11012) was from Thermo Fisher Scientific. DAPI dye (Cat#4083) was from Cell Signaling Technology. RNAi and plasmids 10 pmol siRNA mixture was transfected into

(139) Investigation of the biocompatibility of various pulp capping materials on zebrafish modelPLOS ONESeptember 20, 2024Meltem Karahan, Bahar Basak Kiziltan Eliacik, Umut Cagiral, Evin Iscan, Gunes Ozhanin PBDX_GS) was added. Nuclear staining was carried out using 4′,6-diamidino-2-phenylindole (DAPI; 4083S, Cell Signaling Technology, MA, United States). Then secondary antibody dilution was removed and washed

(140) Chemoproteomics reveals immunogenic and tumor-associated cell surface substrates of ectokinase CK2αCell Chemical BiologySeptember 19, 2024Corleone S. Delaveris, Sophie Kong, Jeff Glasgow, Rita P. Loudermilk, Lisa L. Kirkemo, Fangzhu Zhao, Fernando Salangsang, Paul Phojanakong, Juan Antonio Camara Serrano, Veronica Steri, et al.Cells were then washed three times with PBS, stained with 1 μg/mL DAPI (Cell Signaling Technologies, 4083S) in PBS for 5 min, and then washed three times with PBS before being immersed in Ibidi mounting medium

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DAPI

DAPI from Cell Signaling Technology

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