DAPI from Cell Signaling Technology

Product Specs
ItemDAPI
CompanyCell Signaling Technology
Price $74.00 Inquire
Catalog Number4083S
Quantity1 mg
Research AreaRelated Products
ApplicationsIF-IC, FC-FP

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(121) Baicalein suppresses inflammation and attenuates acute lung injury by inhibiting glycolysis via HIF‑1α signalingMolecular Medicine ReportsNovember 5, 2024Zhongyou Liu, Xiaona Zheng, Ning Li, Zongyao WangCell Signaling Technology, Inc.) was added and incubated at 37°C for 1 h. The sections were incubated with DAPI (cat. no. 4083; Cell Signaling Technology, Inc.) in the dark at room temperature for 5 min for nuclear counterstaining.

(122) Lentiviral vector gene therapy and CFTR modulators show comparable effectiveness in cystic fibrosis rat airway modelsGene therapyNovember 1, 2024McCarron A, Ling KM, Montgomery ST, Martinovich KM, Cmielewski P, Rout-Pitt N, Kicic A, Parsons D, Donnelley M.antibody dilution buffer. The secondary antibody was washed and a DAPI nuclear counterstain was applied (4083S, Cell Signalling Technology). Slides were washed and mounted with ProLong Diamond Antifade mountant

(123) Dietary pro-oxidant therapy by a vitamin K precursor targets PI 3-kinase VPS34 functionScienceOctober 25, 2024Manojit Mosur Swamynathan, Shan Kuang, Kaitlin E. Watrud, Mary R. Doherty, Charlotte Gineste, Grinu Mathew, Grace Q. Gong, Hilary Cox, Eileen Cheng, David Reiss, et al.200 ml of 106 mM MgCl2, 10 ml of 500 mM EDTA at pH 8.0, and 10 mg of DAPI (Cell Signaling Technology, 4083S). Nuclei were prepared by gently centrifuging the cell lysis solution at 1,000 rpm for 5 minutes to

(124) Lysosomal Ca(2+) release-facilitated TFEB nuclear translocation alleviates ischemic brain injury by attenuating autophagic/lysosomal dysfunction in neuronsScientific reportsOctober 22, 2024Lei Q, Chen X, Xiong Y, Li S, Wang J, He H, Deng Y.were labeled for 2 h in the dark and then counterstained with DPAI (1:1000; Cell Signaling Technology, 4083, Danvers, MA, USA). Finally, the reactions were observed and photographed with a fluorescence microscope

(125) Leucine restriction ameliorates Fusobacterium nucleatum-driven malignant progression and radioresistance in nasopharyngeal carcinomaCell Reports MedicineOctober 15, 2024Songhe Guo, Shan Xing, ZhenYu Wu, Fangfang Chen, Xiaoyun Pan, Qifan Li, Wanli Liu, Ge ZhangSR305376 Neomycin,MedChemExpress,Cat# HY-150520; ACS: 1404-04-2 DAPI,Cell Signaling Technology,Cat# 4083 Ci [3 H]-L-leucine,Perkin Elmer,Cat# NET116600 XF 1.0 M Glucose Solution,Agilent Technology,Cat# 103577-100 Seahorse

(126) NPM1 inhibits tumoral antigen presentation to promote immune evasion and tumor progressionJournal of Hematology & OncologyOctober 14, 2024Xin Wang, Yangyang Chai, Yuan Quan, Jiaming Wang, Jiaying Song, Wenkai Zhou, Xiaoqing Xu, Henan Xu, Bingjing Wang, Xuetao CaoFluo 594 anti-rabbit secondary antibody (Cat#A-11012) was from Thermo Fisher Scientific. DAPI dye (Cat#4083) was from Cell Signaling Technology. RNAi and plasmids 10 pmol siRNA mixture was transfected into

(127) Investigation of the biocompatibility of various pulp capping materials on zebrafish modelPLOS ONESeptember 20, 2024Meltem Karahan, Bahar Basak Kiziltan Eliacik, Umut Cagiral, Evin Iscan, Gunes Ozhanin PBDX_GS) was added. Nuclear staining was carried out using 4′,6-diamidino-2-phenylindole (DAPI; 4083S, Cell Signaling Technology, MA, United States). Then secondary antibody dilution was removed and washed

(128) Chemoproteomics reveals immunogenic and tumor-associated cell surface substrates of ectokinase CK2αCell Chemical BiologySeptember 19, 2024Corleone S. Delaveris, Sophie Kong, Jeff Glasgow, Rita P. Loudermilk, Lisa L. Kirkemo, Fangzhu Zhao, Fernando Salangsang, Paul Phojanakong, Juan Antonio Camara Serrano, Veronica Steri, et al.Cells were then washed three times with PBS, stained with 1 μg/mL DAPI (Cell Signaling Technologies, 4083S) in PBS for 5 min, and then washed three times with PBS before being immersed in Ibidi mounting medium

(129) Targeting PRMT3 impairs methylation and oligomerization of HSP60 to boost anti-tumor immunity by activating cGAS/STING signalingNature communicationsSeptember 10, 2024Shi Y, Wu Z, Liu S, Zuo D, Niu Y, Qiu Y, Qiao L, He W, Qiu J, Yuan Y, et al.antibodies and secondary antibodies. The slides were counter-stained with DAPI (Cell Signaling Technology, 4083) for 5 min and enclosed in Antifade Mounting Medium (NobleRyder, I0052). Axioscan7 (ZEISS, Germany)

(130) Antidepressant Effects of Ginsenoside Rc on L-Alpha-Aminoadipic Acid-Induced Astrocytic Ablation and Neuroinflammation in MiceInternational journal of molecular sciencesSeptember 6, 2024Kwon D, Kim Y, Cho SH.for 24 h. Samples were then treated with diluted secondary antibodies, washed, and treated with DAPI (4083S, Cell Signaling Technology, Danvers, MA, USA) for 1 min, followed by a rinse. The samples were then

(131) GABAergic neuronal lineage development determines clinically actionable targets in diffuse hemispheric glioma, H3G34-mutantCancer cellAugust 27, 2024Liu I, Alencastro Veiga Cruzeiro G, Bjerke L, Rogers RF, Grabovska Y, Beck A, Mackay A, Barron T, Hack OA, Quezada MA, et al.stained for Dylight 554 Phalloidin (cytoskeleton) (CST, Cat# 13054, 1:200) and DAPI (nucleus) (CST #4083, final concentration 0.5 μg/ml). Imaging was performed on a Nikon Eclipse Ni C2 Si laser scanning upright

(132) Effect of Mifepristone on Migration and Proliferation of Oral Cancer CellsInternational Journal of Molecular SciencesAugust 12, 2024Anem Iftikhar, Simon Shepherd, Sarah Jones, Ian Ellisone hour at room temperature. This was followed by 3x PBS washes and incubation with 1 µg/mL DAPI (#4083 Cell Signaling Technology, Danvers, MA, USA) for 5 min, to stain the nuclei. DAPI was then removed,

(133) Dedifferentiation-like reprogramming of degenerative nucleus pulposus cells into notochordal-like cells by defined factorsMolecular TherapyAugust 7, 2024Yuang Zhang, Chengzhen Liang, Haibin Xu, Yi Li, Kaishun Xia, Liyin Wang, Xianpeng Huang, Jiangjie Chen, Jiawei Shu, Feng Cheng, et al.with anti-α-tubulin-antibody (Abcam). After nuclei staining using DAPI (Cell Signaling Technology, 4083s), samples were imaged on a laser confocal fluorescence microscopy (Leica, STELL ARI S5, Germany). Cell

(134) Promoting Glutathione Synthesis: A Possibility for Treating Cardiomyopathy Induced by a Maternal Western DietNutrientsAugust 1, 2024Jialing Zhang, Jiayu Wang, Da Xu, Yiting Gui, Fan Bai, Yu Huo, Li Cao, Yonghao Guithe sections were carefully rinsed and subsequently incubated with DAPI solution (1:1000 dilution, #4083, Cell Signaling Technology, Danvers, MA, USA) for an additional 5 min. Once thoroughly rinsed, the sections

(135) In vivo monitoring of active subretinal fibrosis in mice using collagen hybridizing peptidesLab Anim (NY)August 1, 2024Markus Linder, Lucas Bennink, Richard H. Foxton, Mike Kirkness, Peter D. Westenskowday, slides were washed with 1× PBS and incubated with 4′,6-diamidino-2-phenylindole (DAPI; 1:1,000, #4083; Cell Signaling Technology) or, for R-CHP and fibronectin co-staining, with DAPI and donkey anti-rabbit

(136) Modulation of naïve mesenchymal stromal cells by extracellular vesicles derived from insulin-producing cells: an in vitro studyScientific reportsAugust 1, 2024Gabr MM, El-Halawani SM, Refaie AF, Khater SM, Ismail AM, Karras MS, Magar RW, Sayed SE, Kloc M, Uosef A, et al.IgG (H + L) (Cat # 4413, Cell Signaling Technology). The nuclei were counterstained with DAPI (Cat # 4083, Cell Signaling Technology). The cells were plated on chamber slides, and fixed with 4% paraformaldehyde

(137) The in vitro dynamics of pseudo-vascular network formationBritish journal of cancerAugust 1, 2024Oraiopoulou ME, Couturier DL, Bunce EV, Cannell IG, Sweeney PW, Naylor H, Golinska M, Hannon GJ, Sakkalis V, Bohndiek SE.(53-3249-82, Invitrogen, UK), phalloidin conjugated to Alexa Fluor 568 (A12380, Invitrogen, UK), and DAPI (4083, Cell Signalling Technology, UK), as per provider’s recommendations. Images were acquired using a 25x/0.95

(138) TGF-β1 promotes osteogenesis of mesenchymal stem cells via integrin mediated mechanical positive autoregulationiScienceJuly 19, 2024Wan W, Zhang H, Niu L, Zhang M, Xu F, Li A, Pei D, Lin M, Cheng B.,Cell Signaling Technology,Cat# 8890 Alexa Fluor 488,Abcam,ab150077 DAPI,Cell Signaling Technology,Cat# 4083 Lipofectamine® 2000 reagent,Life Technologies,11668019 Opti-MEM medium,Life Technologies,31985070 verteporfin,Sigma,SML0534 ,, Critical

(139) Gastrodin Improves the Activity of the Ubiquitin–Proteasome System and the Autophagy–Lysosome Pathway to Degrade Mutant HuntingtinInternational Journal of Molecular SciencesJuly 14, 2024He Sun, Miao Li, Yunling Li, Na Zheng, Jiaxin Li, Xiang Li, Yingying Liu, Qianyun Ji, Liping Zhou, Jingwen Su, et al.overnight and then incubated with secondary antibodies (Table 2) at 37 °C for 1 h and DAPI (1:10,000, 4083S, Cell Signaling Technology, Danvers, MA, USA) at 37 °C for 10 min. Finally, specimens were examined

(140) Dominant activities of fear engram cells in the dorsal dentate gyrus underlie fear generalization in micePLOS BiologyJuly 12, 2024Kun Cui, Xuetao Qi, Zilong Liu, Weiqi Sun, Peijie Jiao, Chang Liu, Jifu Tong, Xiaoyan Sun, Haojie Sun, Su Fu, et al.Technology,N/A AAV9-TRE3G-mCherry,BrainVTA,N/A AAV9-TRE3G-ChR2-mCherry,BrainVTA,N/A AAV2/9-TRE3G-NpHR-EYFP,BrainVTA,N/A AAV-cfos-tTA-TRE3G-mCherry,BrainVTA,N/A AAV-TRE3G-hM3D(Gq)-mCherry,BrainVTA,N/A AAV2/retro-hSyn-EGFP,BrainVTA,N/A AAV2/5-hSyn-DIO-hM3D(Gq)-EGFP,BrainVTA,N/A AAV2/5-hSyn-DIO-hM4D(Gi)-EGFP,BrainVTA,N/A Chemicals,Chemicals,Chemicals Clozapine-N-oxide,Tocris,CAT # 4936/50 DAPI,Cell Signaling Technology,CAT # 4083S 4-Hydroxytamoxifen (4-OHT),Sigma,CAT # H6278 Experimental models: Organisms/strains,Experimental models:

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DAPI

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DAPI from Cell Signaling Technology

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