DAPI from Cell Signaling Technology

Product Specs
ItemDAPI
CompanyCell Signaling Technology
Price $74.00 Inquire
Catalog Number4083S
Quantity1 mg
Research AreaRelated Products
ApplicationsIF-IC, FC-FP

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(181) Use of recombinant microRNAs as antimetabolites to inhibit human non-small cell lung cancerActa Pharmaceutica Sinica BOctober 1, 2023Yixin Chen, Mei-Juan Tu, Fangwei Han, Zhenzhen Liu, Neelu Batra, Primo N. Lara, Hong-Wu Chen, Huichang Bi, Ai-Ming YuAlexa Fluor 488-conjugated secondary antibody (Thermo Fisher Scientific Cat# A55058). DAPI (Catalog #4083, Cell Signaling Technology) was added to cells to stain the nuclei. All images were obtained by using

(182) HCN2 Channels in the Ventral Hippocampal CA1 Regulate Nociceptive Hypersensitivity in MiceInternational Journal of Molecular SciencesSeptember 7, 2023Yawen Zheng, Shan Shao, Yu Zhang, Shulu Yuan, Yuanwei Xing, Jiaxin Wang, Xuetao Qi, Kun Cui, Jifu Tong, Fengyu Liu, et al.anti-rabbit IgG (1:500, ab150075, Abcam, Cambridge, UK). The brain slices were stained with DAPI (1:1000, 4083S, Cell signaling technology, Danvers, MA, USA). Fluorescence images were captured using a laser scanning

(183) IRP1 mediated ferroptosis reverses temozolomide resistance in glioblastoma via affecting LCN2/FPN1 signaling axis depended on NFKB2iScienceAugust 18, 2023Lan Y, Yang T, Yue Q, Wang Z, Zhong X, Luo X, Zuo B, Zhang M, Zeng T, Liu B, et al.h at room temperature. Nuclear counterstaining was conducted with DAPI (Cell Signaling Technology, #4083, USA). Cells were stained with phalloidin (dilution 1:200; Cell Signaling Technology, #13054, USA) to

(184) Effect of thymosin β4 on lipopolysaccharide‑stimulated brain microvascular endothelial cell remodeling: A possible role in blood‑brain barrier injuryExperimental and Therapeutic MedicineAugust 17, 2023William Stewart, Christina Hejl, Rakeshwar S. Guleria, Sudhiranjan GuptaAnti-ZO3 (cat. no. 3704), anti-occludin (cat. no. 91131), anti-p65 (cat. no. 8242) and DAPI (cat. no. 4083) were purchased from Cell Signaling Technology, Inc. Immunofluorescence-positive cells were evaluated

(185) An original donor-dependent spheroid system for the prediction of idiosyncratic drug-induced liver injury riskIn vitro modelsAugust 15, 2023Sara Cherradi, Nicolas Taulet, Hong Tuan Duong#4413, Cell Signaling) was added for 3 h at room temperature followed by nuclei staining with DAPI (#4083, Cell Signaling). Spheroids were transferred into μ-Slide 8 Well (Ibidi, CliniSciences, Nanterre, France),

(186) RXR Agonists Enhance Lenalidomide Anti-Myeloma Activity and T Cell Functions while Retaining Glucose-Lowering EffectCellsAugust 3, 2023Wu J, Wang X, Zhang M, Mathews P, Kang Y.Thermo Fisher Scientific) for 1 h, labeled with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI; 4083; Cell Signaling) for 5 min, and mounted with the antifade mounting medium (Vector, H-1000). Images were

(187) Inhibition of TANK‐binding kinase1 attenuates the astrocyte‐mediated neuroinflammatory response through YAP signaling after spinal cord injuryCNS Neuroscience & TherapeuticsAugust 1, 2023Wenbin Zhang, Mengxian Jia, Jiashu Lian, Sheng Lu, Jian Zhou, Ziwei Fan, Zhoule Zhu, Yaozhi He, Changgang Huang, Mingyu Zhu, et al.mouse anti‐Iba1 (1:100, GB12105, Servicebio). Meanwhile, the nucleus was marked with DAPI (1:1000, #4083, CST). Images were captured using a confocal laser scanning microscope (FV3000RS, Olympus) or a fluorescence microscope

(188) Colitis Is Associated with Loss of the Histidine Phosphatase LHPP and Upregulation of Histidine Phosphorylation in Intestinal Epithelial CellsBiomedicinesAugust 1, 2023Linder M, Liko D, Kancherla V, Piscuoglio S, Hall MN.(Alexa Fluor 488 anti-rabbit and Alexa Fluor 568 anti-rat; Invitrogen, Waltham, MA, USA) and DAPI (4083; CST, Danvers, MA, USA). Finally, the stained sections were washed and mounted with a water-based mounting

(189) LncRNA LINC01871 sponging miR-142-3p to modulate ZYG11B promotes the chemoresistance of colorectal cancer cells by inducing autophagyAnti-Cancer DrugsAugust 1, 2023Bensong Duan, Haibin Zhang, Zhen Zhu, Xiaohan Yan, Zhonghua Ji, Jingze Lifor 2 h away from light. Finally, the nucleus was stained using 4',6-diamidino-2-phenylindole (DAPI; 4083S, CST), and the images of cells were captured by the fluorescence microscope (Olympus BX51). The amount

(190) Study of the antioxidant and anti-pancreatic cancer activities of Anchusa strigosa aqueous extracts obtained by maceration and ultrasonic extraction techniquesFrontiers in PharmacologyAugust 1, 2023Ziad Chebaro, Rola Abdallah, Adnan Badran, Kamar Hamade, Akram Hijazi, Marc Maresca, Joelle Edward Mesmar, Elias Baydounand ASU for 24 h. Then the cells were fixed with 4% formaldehyde, stained with DAPI (Cell Signaling #4083) according to the manufacturer’s instructions, and visualized by fluorescence microscopy. 2.9 Wound-healing

(191) Prenatal Exposure to General Anesthesia Drug Esketamine Impaired Neurobehavior in OffspringCellular and Molecular NeurobiologyAugust 1, 2023Ronghua Huang, Bingbiao Lin, Hongyan Tian, Qichen Luo, Yalan Lianti-rabbit 546 IgG (1:1000; Thermo Fisher, USA, A10040). DAPI (1:1000; Cell Signaling Technology, 4083) was used for staining the nucleus. The EdU-positive test was accomplished with the help of an EdU imaging

(192) ARPC5 deficiency leads to severe early-onset systemic inflammation and mortalityDisease models & mechanismsJuly 1, 2023Sindram E, Caballero-Oteyza A, Kogata N, Chor Mei Huang S, Alizadeh Z, Gámez-Díaz L, Fazlollhi MR, Peng X, Grimbacher B, Way M, et al.temperature. After three washes in 1× PBS, cells were incubated with DAPI (Cell Signaling Technology, 4083S, 300 nM) for 5 mins. Coverslips were then washed twice with 1× PBS and once with distilled water before

(193) Inherited ARPC5 mutations cause an actinopathy impairing cell motility and disrupting cytokine signalingNature communicationsJune 22, 2023Nunes-Santos CJ, Kuehn H, Boast B, Hwang S, Kuhns DB, Stoddard J, Niemela JE, Fink DL, Pittaluga S, Abu-Asab M, et al.A11072 and A21069). After washes, all samples were incubated with DAPI (Cell Signaling Technology, Cat. 4083) in PBS, for 10 min. Samples were washed twice in PBS and ready for imaging. Filopodia formation was

(194) Pseudoknot-targeting Cas13b combats SARS-CoV-2 infection by suppressing viral replicationMolecular therapy : the journal of the American Society of Gene TherapyJune 7, 2023Yu D, Han HJ, Yu J, Kim J, Lee GH, Yang JH, Song BM, Tark D, Choi BS, Kang SM, et al.Cell Signaling Technology) were used to detect viral proteins and 4′,6′-diamidino-2-phenylindole (DAPI) (no. 4083S; Cell Signaling Technology) stained the nucleus. Fluorescent images were analyzed using the CELENA S digital imaging system

(195) Tetrandrine citrate suppresses lung adenocarcinoma growth via SLC7A11/GPX4-mediated ferroptosisDiscover OncologyJune 2, 2023Xiaocong Mo, Di Hu, Kaisheng Yuan, Juyu Luo, Cheng Huang, Meng Xu(Rabbit: #4417; 1:600; CST; USA. Mouse: #4408; 1:600; CST; USA) for 60 min, and then stained with DAPI (#4083; CST; USA) for 15 min in the dark. Finally, a high-sensitivity confocal microscope (Nikon A1R/A1) was

(196) Synaptic scaling of corticostriatal circuits underlies hyperactivity in GABA Transporter-1 deficient miceiScienceApril 21, 2023Wu YJ, Yi X, Gu X, Wang Q, Jiang Q, Li Y, Zhu MX, Ding J, Li WG, Xu TL.proteins","Chemicals, peptides, and recombinant proteins" ,, DAPI,Cell Signaling Technology,Cat# 4083 NASPM,MedChemExpress,Cat# HY-12506 CDPPB,TOCRIS,Cat# 3235 "WIN 55,212-2",TOCRIS,Cat# 1038 PTX,Sigma,Cat#

(197) Ablation of Mea6/cTAGE5 in oligodendrocytes significantly impairs white matter structure and lipid contentLife MetabolismApril 1, 2023Tiantian Ma, Wei Mao, Shaohua Zhang, Yaqing Wang, Tao Wang, Jinghua Liu, Lei Shi, Xiang Yu, Rong Xue, Guanghou Shui, et al.Nuclei were counterstained with DAPI (4ʹ,6-diamidino-2-phenylindole) (Cell Signaling Technology, Cat: 4083s, 1:10000) when incubated with secondary antibody. Oligodendrocyte progenitor cell (OPC) isolation,

(198) SIRT7 Downregulation Promotes Breast Cancer Metastasis Via LAP2α-Induced Chromosomal InstabilityInternational Journal of Biological SciencesMarch 5, 2023Qin Huo, Siqi Chen, Jialang Zhuang, Chuntao Quan, Yue Wang, Ni Xiesecondary antibodies coupled to Alexa Fluor, and stained the nuclei by incubating with 1 μg/mL DAPI (CST, 4083S) for 5 min. The primary antibodies used were as follows: SIRT7 (1:500; Abcam, ab62748), γH2AX (1:400;

(199) Inhibition of Sphingosine Kinase 2 Results in PARK2-Mediated Mitophagy and Induces Apoptosis in Multiple MyelomaCurrent oncology (Toronto, Ont.)March 4, 2023Wu J, Fan S, Feinberg D, Wang X, Jabbar S, Kang Y.for 1 h. After washing them three times with PBS, the slides were stained with DAPI (Cell Signaling, 4083) for 5 min and mounted with an antifade mounting medium (Vector, H-1000). Images were acquired using

(200) High cell density and high-resolution 3D bioprinting for fabricating vascularized tissuesScience advancesFebruary 22, 2023You S, Xiang Y, Hwang HH, Berry DB, Kiratitanaporn W, Guan J, Yao E, Tang M, Zhong Z, Ma X, et al.Cytoskeleton and nuclei were labeled with Phalloidin eFluor 660 (50-6559-05, eBioscience) and DAPI (4083S, Cell Signaling Technology) per the manufacturer’s instruction before the slides were mounted with antifade

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