DAPI from Cell Signaling Technology

Product Specs
ItemDAPI
CompanyCell Signaling Technology
Price $74.00 Inquire
Catalog Number4083S
Quantity1 mg
Research AreaRelated Products
ApplicationsIF-IC, FC-FP

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(181) Integration of xeno-free single-cell cloning in CRISPR-mediated DNA editing of human iPSCs improves homogeneity and methodological efficiency of cellular disease modelingStem Cell ReportsDecember 12, 2023Atefeh Namipashaki, Kealan Pugsley, Xiaodong Liu, Kirra Abrehart, Sue Mei Lim, Guizhi Sun, Marco J. Herold, Jose M. Polo, Mark A. Bellgrove, Ziarih Hawipenicillin-streptomycin (Gibco, cat. no. 15140122), 5 μL of DAPI (Cell Signaling Technology, cat. no. 4083S), and 2 μL of 0.5 M EDTA (Invitrogen, cat. no. 15575020) and passed through a Falcon 5 mL tube with

(182) Sorting nexin 3 exacerbates doxorubicin-induced cardiomyopathy via regulation of TFRC-dependent ferroptosisActa Pharmaceutica Sinica BDecember 1, 2023Wenjing Yu, Yuehuai Hu, Zhiping Liu, Kaiteng Guo, Dinghu Ma, Mingxia Peng, Yuemei Wang, Jing Zhang, Xiaolei Zhang, Panxia Wang, et al.with fluorescent secondary antibodies (4412S, 8890S; CST) for 1 h at room temperature. Finally, DAPI (4083; CST) was used to label the nuclei. Fluorescence was detected below the FV3000 fluorescent microscope

(183) KCNQ potassium channels modulate Wnt activity in gastro-oesophageal adenocarcinomasLife science allianceDecember 1, 2023Shorthouse D, Zhuang L, Rahrmann EP, Kosmidou C, Wickham Rahrmann K, Hall M, Greenwood BM, Devonshire G, Gilbertson RJ, Fitzgerald RC, et al.for non-specific staining of each antibody. Sections were then counterstained using DAPI (1:10,000, 4083; Cell Signaling) and mounted using ProLong Gold Antifade Mountant (P36930; Thermo Fisher Scientific).

(184) Epigenetically Downregulated Breast Cancer Gene 2 through Acetyltransferase Lysine Acetyltransferase 2B Increases the Sensitivity of Colorectal Cancer to OlaparibCancers (Basel)November 25, 2023Siche Chen, Heike Allgayerh at room temperature in the dark, followed by setting with DAPI (Cell Signaling, Danvers, MA, USA, 4083S) for 20 min. Coverslips were washed and mounted in an anti-fade mounting medium. The fluorescence images

(185) Activation of KrasG12D in Subset of Alveolar Type II Cells Enhances Cellular Plasticity in Lung AdenocarcinomaCancer Res CommunNovember 24, 2023Chaudhary P, Xu X, Wang G, Hoj JP, Rampersad RR, Asselin-Labat ML, Ting S, Kim W, Tamayo P, Pendergast AM, et al.Fluor-coupled secondary antibodies (Invitrogen) were used at 1:500 dilution. DAPI (Cell Signaling Technology, 4083) was used to stain nuclei at a concentration of 0.1 mmol/L. Images were captured on a Leica Sp2 laser

(186) 5′-Methylthioadenosine strongly suppresses RANKL-induced osteoclast differentiation and function via inhibition of RANK-NFATc1 signalling pathwaysHeliyonNovember 18, 2023Purithat Rattajak, Aratee Aroonkesorn, Carl Smythe, Rapepun Wititsuwannakul, Thanawat PitakpornpreechaSanta Cruz Biotechnology, Dallas, TX, USA) was added and incubated for 3 h and counterstained with DAPI (1 μg/mL) (No. 4083, Cell Signaling Technology, Danvers, MA) for 5 min. Lastly, a mounting medium was carefully added to

(187) Regulation of Drp1 and enhancement of mitochondrial fission by the deubiquitinating enzyme PSMD14 facilitates the proliferation of bladder cancer cellsOncology ReportsNovember 16, 2023Wei Song, Zhuo Li, Ming Xia, Wei XiaoCell Signaling Technology, Inc.), or anti-TOM20 (cat. no. 42406S; 1:200; Cell Signaling Technology, Inc.). DAPI (cat. no. 4083S; Cell Signaling Technology, Inc. 1:200) was used to counterstain the nuclei. The secondary antibodies

(188) Canonical Wnt and TGF-β/BMP signaling enhance melanocyte regeneration but suppress invasiveness, migration, and proliferation of melanoma cellsFrontiers in Cell and Developmental BiologyNovember 13, 2023Esra Katkat, Yeliz Demirci, Guillaume Heger, Doga Karagulle, Irene Papatheodorou, Alvis Brazma, Gunes OzhanJackson Immunoresearch Laboratories, PA, United States). Nuclear staining was carried out using 4′,6-diamidino-2-phenylindole (DAPI; 4083S, Cell Signaling Technology, MA, United States). Larvae were imaged using fluorescence confocal microscopy. Confocal

(189) FAM222A, part of the BET-regulated basal endothelial transcriptome, is a novel determinant of endothelial biology and angiogenesisArterioscler Thromb Vasc BiolNovember 9, 2023Aspasia Tzani, Stefan Haemmig, Henry S. Cheng, Daniel Perez-Cremades, Marina Augusto Heuschkel, Anurag Jamaiyar, Sasha Singh, Masanori Aikawa, Paul Yu, Tianxi Wang, et al.Thermo Fisher). Cell nuclei were stained with 4′,6-diamidino- 2-phenylindole (DAPI, 0.5 ug/ml, Cat#: 4083, Cell Signaling). Retinas were imaged using a Zeiss AxioObserver.Z1 microscope and AxioCam MRm camera.

(190) NUAK1 promotes tumor metastasis through upregulating slug transcription in esophageal squamous cell carcinomaCancer Cell InternationalNovember 2, 2023Huiru Yang, Zhen Wei, Yifan Song, Kexin Du, Nannan Yin, Hong Lu, Bingbing Li, Lili Hou, Panfei Xing, Liang Chen, et al.room temperature. After washing thrice with PBS, the cells were by incubation for 5 min with DAPI (#4083, 0.5 µg/mL, CST) to label the nuclei. Images were captured using a confocal scanning microscope (Leica

(191) Comparative membrane lipidomics of hepatocellular carcinoma cells reveals diacylglycerol and ceramide as key regulators of Wnt/β‐catenin signaling and tumor growthMolecular OncologyNovember 1, 2023Yagmur Azbazdar, Yeliz Demirci, Guillaume Heger, Dogac Ipekgil, Mustafa Karabicici, Gunes Ozhan5 min each, their nuclei were counterstained with 4′,6‐diamidino‐2‐phenylindole (DAPI, 0.5 μg·mL−1, 4083S, Cell Signaling Technology, MA, USA) and imaged by using an LSM 880 laser scanning confocal microscope

(192) Addition of exogenous diacylglycerol enhances Wnt/β-catenin signaling through stimulation of macropinocytosisiScienceOctober 20, 2023Azbazdar Y, Tejeda-Munoz N, Monka JC, Dayrit A, Binder G, Ozhan G, De Robertis EM.times and were mounted using mounting medium including 4′,6-diamidino-2-phenylindole (DAPI, 0.5 ug/ml, 4083S, Cell Signaling Technology, MA, USA). Immunostainings were imaged with a Carl Zeiss Axio Observer Z1

(193) Plasma 8-OHdG act as a biomarker for steroid-induced osteonecrosis of the femoral headBMC Musculoskeletal DisordersOctober 12, 2023Peng Peng, Mincong He, Weihua Fang, Mengqi Lai, Fangjun Xiao, Wei He, Huan Xiao, Qiushi Wei37°C for 2 h. The slices were stained with DAPI nuclear staining (Cell Signaling Technology, cat. #4083) for 1 min. Use the same antibody as for Immunoshistochemistry. Measurement of 8-OHdG concentrations Plasma

(194) Use of recombinant microRNAs as antimetabolites to inhibit human non-small cell lung cancerActa Pharmaceutica Sinica BOctober 1, 2023Yixin Chen, Mei-Juan Tu, Fangwei Han, Zhenzhen Liu, Neelu Batra, Primo N. Lara, Hong-Wu Chen, Huichang Bi, Ai-Ming YuAlexa Fluor 488-conjugated secondary antibody (Thermo Fisher Scientific Cat# A55058). DAPI (Catalog #4083, Cell Signaling Technology) was added to cells to stain the nuclei. All images were obtained by using

(195) HCN2 Channels in the Ventral Hippocampal CA1 Regulate Nociceptive Hypersensitivity in MiceInternational Journal of Molecular SciencesSeptember 7, 2023Yawen Zheng, Shan Shao, Yu Zhang, Shulu Yuan, Yuanwei Xing, Jiaxin Wang, Xuetao Qi, Kun Cui, Jifu Tong, Fengyu Liu, et al.anti-rabbit IgG (1:500, ab150075, Abcam, Cambridge, UK). The brain slices were stained with DAPI (1:1000, 4083S, Cell signaling technology, Danvers, MA, USA). Fluorescence images were captured using a laser scanning

(196) IRP1 mediated ferroptosis reverses temozolomide resistance in glioblastoma via affecting LCN2/FPN1 signaling axis depended on NFKB2iScienceAugust 18, 2023Lan Y, Yang T, Yue Q, Wang Z, Zhong X, Luo X, Zuo B, Zhang M, Zeng T, Liu B, et al.h at room temperature. Nuclear counterstaining was conducted with DAPI (Cell Signaling Technology, #4083, USA). Cells were stained with phalloidin (dilution 1:200; Cell Signaling Technology, #13054, USA) to

(197) Effect of thymosin β4 on lipopolysaccharide‑stimulated brain microvascular endothelial cell remodeling: A possible role in blood‑brain barrier injuryExperimental and Therapeutic MedicineAugust 17, 2023William Stewart, Christina Hejl, Rakeshwar S. Guleria, Sudhiranjan GuptaAnti-ZO3 (cat. no. 3704), anti-occludin (cat. no. 91131), anti-p65 (cat. no. 8242) and DAPI (cat. no. 4083) were purchased from Cell Signaling Technology, Inc. Immunofluorescence-positive cells were evaluated

(198) An original donor-dependent spheroid system for the prediction of idiosyncratic drug-induced liver injury riskIn vitro modelsAugust 15, 2023Sara Cherradi, Nicolas Taulet, Hong Tuan Duong#4413, Cell Signaling) was added for 3 h at room temperature followed by nuclei staining with DAPI (#4083, Cell Signaling). Spheroids were transferred into μ-Slide 8 Well (Ibidi, CliniSciences, Nanterre, France),

(199) RXR Agonists Enhance Lenalidomide Anti-Myeloma Activity and T Cell Functions while Retaining Glucose-Lowering EffectCellsAugust 3, 2023Wu J, Wang X, Zhang M, Mathews P, Kang Y.Thermo Fisher Scientific) for 1 h, labeled with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI; 4083; Cell Signaling) for 5 min, and mounted with the antifade mounting medium (Vector, H-1000). Images were

(200) Colitis Is Associated with Loss of the Histidine Phosphatase LHPP and Upregulation of Histidine Phosphorylation in Intestinal Epithelial CellsBiomedicinesAugust 1, 2023Linder M, Liko D, Kancherla V, Piscuoglio S, Hall MN.(Alexa Fluor 488 anti-rabbit and Alexa Fluor 568 anti-rat; Invitrogen, Waltham, MA, USA) and DAPI (4083; CST, Danvers, MA, USA). Finally, the stained sections were washed and mounted with a water-based mounting

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