DAPI from Cell Signaling Technology

Product Specs
ItemDAPI
CompanyCell Signaling Technology
Price $74.00 Inquire
Catalog Number4083S
Quantity1 mg
Research AreaRelated Products
ApplicationsIF-IC, FC-FP

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

Website: Technical Support

(141) Targeting PRMT3 impairs methylation and oligomerization of HSP60 to boost anti-tumor immunity by activating cGAS/STING signalingNature communicationsSeptember 10, 2024Shi Y, Wu Z, Liu S, Zuo D, Niu Y, Qiu Y, Qiao L, He W, Qiu J, Yuan Y, et al.antibodies and secondary antibodies. The slides were counter-stained with DAPI (Cell Signaling Technology, 4083) for 5 min and enclosed in Antifade Mounting Medium (NobleRyder, I0052). Axioscan7 (ZEISS, Germany)

(142) Antidepressant Effects of Ginsenoside Rc on L-Alpha-Aminoadipic Acid-Induced Astrocytic Ablation and Neuroinflammation in MiceInternational journal of molecular sciencesSeptember 6, 2024Kwon D, Kim Y, Cho SH.for 24 h. Samples were then treated with diluted secondary antibodies, washed, and treated with DAPI (4083S, Cell Signaling Technology, Danvers, MA, USA) for 1 min, followed by a rinse. The samples were then

(143) GABAergic neuronal lineage development determines clinically actionable targets in diffuse hemispheric glioma, H3G34-mutantCancer cellAugust 27, 2024Liu I, Alencastro Veiga Cruzeiro G, Bjerke L, Rogers RF, Grabovska Y, Beck A, Mackay A, Barron T, Hack OA, Quezada MA, et al.stained for Dylight 554 Phalloidin (cytoskeleton) (CST, Cat# 13054, 1:200) and DAPI (nucleus) (CST #4083, final concentration 0.5 μg/ml). Imaging was performed on a Nikon Eclipse Ni C2 Si laser scanning upright

(144) Effect of Mifepristone on Migration and Proliferation of Oral Cancer CellsInternational Journal of Molecular SciencesAugust 12, 2024Anem Iftikhar, Simon Shepherd, Sarah Jones, Ian Ellisone hour at room temperature. This was followed by 3x PBS washes and incubation with 1 µg/mL DAPI (#4083 Cell Signaling Technology, Danvers, MA, USA) for 5 min, to stain the nuclei. DAPI was then removed,

(145) Dedifferentiation-like reprogramming of degenerative nucleus pulposus cells into notochordal-like cells by defined factorsMolecular TherapyAugust 7, 2024Yuang Zhang, Chengzhen Liang, Haibin Xu, Yi Li, Kaishun Xia, Liyin Wang, Xianpeng Huang, Jiangjie Chen, Jiawei Shu, Feng Cheng, et al.with anti-α-tubulin-antibody (Abcam). After nuclei staining using DAPI (Cell Signaling Technology, 4083s), samples were imaged on a laser confocal fluorescence microscopy (Leica, STELL ARI S5, Germany). Cell

(146) The in vitro dynamics of pseudo-vascular network formationBritish journal of cancerAugust 1, 2024Oraiopoulou ME, Couturier DL, Bunce EV, Cannell IG, Sweeney PW, Naylor H, Golinska M, Hannon GJ, Sakkalis V, Bohndiek SE.(53-3249-82, Invitrogen, UK), phalloidin conjugated to Alexa Fluor 568 (A12380, Invitrogen, UK), and DAPI (4083, Cell Signalling Technology, UK), as per provider’s recommendations. Images were acquired using a 25x/0.95

(147) Modulation of naïve mesenchymal stromal cells by extracellular vesicles derived from insulin-producing cells: an in vitro studyScientific reportsAugust 1, 2024Gabr MM, El-Halawani SM, Refaie AF, Khater SM, Ismail AM, Karras MS, Magar RW, Sayed SE, Kloc M, Uosef A, et al.IgG (H + L) (Cat # 4413, Cell Signaling Technology). The nuclei were counterstained with DAPI (Cat # 4083, Cell Signaling Technology). The cells were plated on chamber slides, and fixed with 4% paraformaldehyde

(148) In vivo monitoring of active subretinal fibrosis in mice using collagen hybridizing peptidesLab Anim (NY)August 1, 2024Markus Linder, Lucas Bennink, Richard H. Foxton, Mike Kirkness, Peter D. Westenskowday, slides were washed with 1× PBS and incubated with 4′,6-diamidino-2-phenylindole (DAPI; 1:1,000, #4083; Cell Signaling Technology) or, for R-CHP and fibronectin co-staining, with DAPI and donkey anti-rabbit

(149) Promoting Glutathione Synthesis: A Possibility for Treating Cardiomyopathy Induced by a Maternal Western DietNutrientsAugust 1, 2024Jialing Zhang, Jiayu Wang, Da Xu, Yiting Gui, Fan Bai, Yu Huo, Li Cao, Yonghao Guithe sections were carefully rinsed and subsequently incubated with DAPI solution (1:1000 dilution, #4083, Cell Signaling Technology, Danvers, MA, USA) for an additional 5 min. Once thoroughly rinsed, the sections

(150) TGF-β1 promotes osteogenesis of mesenchymal stem cells via integrin mediated mechanical positive autoregulationiScienceJuly 19, 2024Wan W, Zhang H, Niu L, Zhang M, Xu F, Li A, Pei D, Lin M, Cheng B.,Cell Signaling Technology,Cat# 8890 Alexa Fluor 488,Abcam,ab150077 DAPI,Cell Signaling Technology,Cat# 4083 Lipofectamine® 2000 reagent,Life Technologies,11668019 Opti-MEM medium,Life Technologies,31985070 verteporfin,Sigma,SML0534 ,, Critical

(151) Gastrodin Improves the Activity of the Ubiquitin–Proteasome System and the Autophagy–Lysosome Pathway to Degrade Mutant HuntingtinInternational Journal of Molecular SciencesJuly 14, 2024He Sun, Miao Li, Yunling Li, Na Zheng, Jiaxin Li, Xiang Li, Yingying Liu, Qianyun Ji, Liping Zhou, Jingwen Su, et al.overnight and then incubated with secondary antibodies (Table 2) at 37 °C for 1 h and DAPI (1:10,000, 4083S, Cell Signaling Technology, Danvers, MA, USA) at 37 °C for 10 min. Finally, specimens were examined

(152) Dominant activities of fear engram cells in the dorsal dentate gyrus underlie fear generalization in micePLOS BiologyJuly 12, 2024Kun Cui, Xuetao Qi, Zilong Liu, Weiqi Sun, Peijie Jiao, Chang Liu, Jifu Tong, Xiaoyan Sun, Haojie Sun, Su Fu, et al.Technology,N/A AAV9-TRE3G-mCherry,BrainVTA,N/A AAV9-TRE3G-ChR2-mCherry,BrainVTA,N/A AAV2/9-TRE3G-NpHR-EYFP,BrainVTA,N/A AAV-cfos-tTA-TRE3G-mCherry,BrainVTA,N/A AAV-TRE3G-hM3D(Gq)-mCherry,BrainVTA,N/A AAV2/retro-hSyn-EGFP,BrainVTA,N/A AAV2/5-hSyn-DIO-hM3D(Gq)-EGFP,BrainVTA,N/A AAV2/5-hSyn-DIO-hM4D(Gi)-EGFP,BrainVTA,N/A Chemicals,Chemicals,Chemicals Clozapine-N-oxide,Tocris,CAT # 4936/50 DAPI,Cell Signaling Technology,CAT # 4083S 4-Hydroxytamoxifen (4-OHT),Sigma,CAT # H6278 Experimental models: Organisms/strains,Experimental models:

(153) TET2–STAT3–CXCL5 nexus promotes neutrophil lipid transfer to fuel lung adeno-to-squamous transitionJournal of Experimental MedicineJuly 1, 2024Yun Xue, Yuting Chen, Sijia Sun, Xinyuan Tong, Yujia Chen, Shijie Tang, Xue Wang, Simin Bi, Yuqin Qiu, Qiqi Zhao, et al.pSTAT3 (9145; CST), MPO (AF3667; R&D system), BODIPY (GC42959; GLPBIO), EpCAM (2929; CST), and DAPI (4083; CST). Western blot Tissues or cell lysates were prepared for western blot analysis with the following

(154) Notoginsenoside R1 promotes Lgr5+ stem cell and epithelium renovation in colitis mice via activating Wnt/β-Catenin signalingActa Pharmacologica SinicaJuly 1, 2024Zhi-lun Yu, Rui-yang Gao, Cheng Lv, Xiao-long Geng, Yi-jing Ren, Jing Zhang, Jun-yu Ren, Hao Wang, Fang-bin Ai, Zi-yi Wang, et al.conjugated to fluorophores (9300039001, ABclonal, Wuhan, China). Nuclei were counterstained with DAPI (#4083, CST, MA, USA), and images were obtained using a fluorescence microscope (BX61VS, Olympus, Tokyo, Japan).

(155) ARID1A is involved in DNA double-strand break repair in gastric cancerJournal of Gastrointestinal OncologyJune 30, 2024Ying Zhang, He-Sheng Qian, Gengwei Hu, Lu Wang, Yiping Zhu(#4412s; Cell Signaling Technology) as the secondary antibody. 4’,6-diamidino-2-phenylindole (DAPI) (#4083s; Cell Signaling Technology) was used to stain cellular nuclei. Finally, fluorescence was visualized

(156) Protocol for the cryopreservation of Cryptosporidium isolates using enteroidsSTAR ProtocolsJune 21, 2024Miner Deng, Yanting Wei, Na Li, Lihua Xiao, Yaoyu Feng, Yaqiong Guo8.0",Beyotime,Cat#ST066 4% Paraformaldehyde,Leagene,Cat#DF0135 Triton X-100,Sigma,Cat#9002-93-1 "DAPI (1:2,000)",CST,Cat#4083S Bleach,Clorox,N/A Sodium taurocholate,Aladdin,Cat#145-42-6 RPMI 1640 medium,Gibco,Cat#11875093 Advanced DMEM/F-12

(157) Intrinsic and extrinsic determinants of conditional localization of Mms6 to magnetosome organelles in Magnetospirillum magneticum AMB-1Journal of BacteriologyJune 20, 2024Carson D. Bickley, Juan Wan, Arash Komeilimedium and stained with 1.4 µM 4′,6-diamidino-2-phenylindole (DAPI) from Cell Signaling Technology (Cat #4083S) for 15 min in the dark at 30°C in the microaerobic chamber. FM 1-43 (Life Technologies Corporation

(158) TNBC response to paclitaxel phenocopies interferon response which reveals cell cycle-associated resistance mechanismsbioRxivJune 11, 2024Nicholas L Calistri, Tiera A. Liby, Zhi Hu, Hongmei Zhang, Mark Dane, Sean M. Gross, Laura M. HeiserAbcam) overnight at 4C. Each well was washed twice with room temp PBS then stained with 0.5ug/mL DAPI (4083S, Cell Signaling Technology) in PBS for 15 minutes at room temperature. Following DAPI staining, wells

(159) Single‐Nucleus Multiomic Analyses Identifies Gene Regulatory Dynamics of Phenotypic Modulation in Human Aneurysmal Aortic RootAdvanced ScienceJune 1, 2024Xuanyu Liu, Qingyi Zeng, Hang Yang, Wenke Li, Qianlong Chen, Kunlun Yin, Zihang Pan, Kai Wang, Mingyao Luo, Chang Shu, et al.R415, Thermo Fisher Scientific) for 60 min at room temperature. The nuclei were stained with DAPI (4083S, Cell Signaling Technology). H&E and EVG Staining The sections of formaldehyde‐fixed paraffin‐embedded

(160) Effect of hypercholesterolemia on circulating and cardiomyocyte-derived extracellular vesiclesScientific reportsMay 26, 2024Kovácsházi C, Hambalkó S, Sayour NV, Gergely TG, Brenner GB, Pelyhe C, Kapui D, Weber BY, Hültenschmidt AL, Pállinger É, et al.were labeled with 1:1.000 dilution of DAPI (Cell Signaling Technologies, Danvers, MA, USA, Cat. No.: 4083S) for 1 min, and the samples were covered with Prolong Gold (Thermo Scientific, Waltham, MA, USA) mounting

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DAPI

DAPI from Cell Signaling Technology

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DAPI from Cell Signaling Technology

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