DAPI from Cell Signaling Technology

Product Specs
ItemDAPI
CompanyCell Signaling Technology
Price $74.00 Inquire
Catalog Number4083S
Quantity1 mg
Research AreaRelated Products
ApplicationsIF-IC, FC-FP

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(141) TET2–STAT3–CXCL5 nexus promotes neutrophil lipid transfer to fuel lung adeno-to-squamous transitionJournal of Experimental MedicineJuly 1, 2024Yun Xue, Yuting Chen, Sijia Sun, Xinyuan Tong, Yujia Chen, Shijie Tang, Xue Wang, Simin Bi, Yuqin Qiu, Qiqi Zhao, et al.pSTAT3 (9145; CST), MPO (AF3667; R&D system), BODIPY (GC42959; GLPBIO), EpCAM (2929; CST), and DAPI (4083; CST). Western blot Tissues or cell lysates were prepared for western blot analysis with the following

(142) Notoginsenoside R1 promotes Lgr5+ stem cell and epithelium renovation in colitis mice via activating Wnt/β-Catenin signalingActa Pharmacologica SinicaJuly 1, 2024Zhi-lun Yu, Rui-yang Gao, Cheng Lv, Xiao-long Geng, Yi-jing Ren, Jing Zhang, Jun-yu Ren, Hao Wang, Fang-bin Ai, Zi-yi Wang, et al.conjugated to fluorophores (9300039001, ABclonal, Wuhan, China). Nuclei were counterstained with DAPI (#4083, CST, MA, USA), and images were obtained using a fluorescence microscope (BX61VS, Olympus, Tokyo, Japan).

(143) ARID1A is involved in DNA double-strand break repair in gastric cancerJournal of Gastrointestinal OncologyJune 30, 2024Ying Zhang, He-Sheng Qian, Gengwei Hu, Lu Wang, Yiping Zhu(#4412s; Cell Signaling Technology) as the secondary antibody. 4’,6-diamidino-2-phenylindole (DAPI) (#4083s; Cell Signaling Technology) was used to stain cellular nuclei. Finally, fluorescence was visualized

(144) Protocol for the cryopreservation of Cryptosporidium isolates using enteroidsSTAR ProtocolsJune 21, 2024Miner Deng, Yanting Wei, Na Li, Lihua Xiao, Yaoyu Feng, Yaqiong Guo8.0",Beyotime,Cat#ST066 4% Paraformaldehyde,Leagene,Cat#DF0135 Triton X-100,Sigma,Cat#9002-93-1 "DAPI (1:2,000)",CST,Cat#4083S Bleach,Clorox,N/A Sodium taurocholate,Aladdin,Cat#145-42-6 RPMI 1640 medium,Gibco,Cat#11875093 Advanced DMEM/F-12

(145) Intrinsic and extrinsic determinants of conditional localization of Mms6 to magnetosome organelles in Magnetospirillum magneticum AMB-1Journal of BacteriologyJune 20, 2024Carson D. Bickley, Juan Wan, Arash Komeilimedium and stained with 1.4 µM 4′,6-diamidino-2-phenylindole (DAPI) from Cell Signaling Technology (Cat #4083S) for 15 min in the dark at 30°C in the microaerobic chamber. FM 1-43 (Life Technologies Corporation

(146) TNBC response to paclitaxel phenocopies interferon response which reveals cell cycle-associated resistance mechanismsbioRxivJune 11, 2024Nicholas L Calistri, Tiera A. Liby, Zhi Hu, Hongmei Zhang, Mark Dane, Sean M. Gross, Laura M. HeiserAbcam) overnight at 4C. Each well was washed twice with room temp PBS then stained with 0.5ug/mL DAPI (4083S, Cell Signaling Technology) in PBS for 15 minutes at room temperature. Following DAPI staining, wells

(147) Single‐Nucleus Multiomic Analyses Identifies Gene Regulatory Dynamics of Phenotypic Modulation in Human Aneurysmal Aortic RootAdvanced ScienceJune 1, 2024Xuanyu Liu, Qingyi Zeng, Hang Yang, Wenke Li, Qianlong Chen, Kunlun Yin, Zihang Pan, Kai Wang, Mingyao Luo, Chang Shu, et al.R415, Thermo Fisher Scientific) for 60 min at room temperature. The nuclei were stained with DAPI (4083S, Cell Signaling Technology). H&E and EVG Staining The sections of formaldehyde‐fixed paraffin‐embedded

(148) Effect of hypercholesterolemia on circulating and cardiomyocyte-derived extracellular vesiclesScientific reportsMay 26, 2024Kovácsházi C, Hambalkó S, Sayour NV, Gergely TG, Brenner GB, Pelyhe C, Kapui D, Weber BY, Hültenschmidt AL, Pállinger É, et al.were labeled with 1:1.000 dilution of DAPI (Cell Signaling Technologies, Danvers, MA, USA, Cat. No.: 4083S) for 1 min, and the samples were covered with Prolong Gold (Thermo Scientific, Waltham, MA, USA) mounting

(149) Drosophila Evi5 is a critical regulator of intracellular iron transport via transferrin and ferritin interactionsNature CommunicationsMay 14, 2024Sattar Soltani, Samuel M. Webb, Thomas Kroll, Kirst King-Jonesthen washed three additional times in PBST and mounted in VECTASHIELD mounting medium (Cell signaling #4083). All images were taken with A Nikon C2si Confocal Microscope. LysoTracker Blue staining For lysosome

(150) Pleurotus sajor-caju (Fr.) Singer β-1,3-Glucanoligosaccharide (Ps-GOS) Suppresses RANKL-Induced Osteoclast Differentiation and Function in Pre-Osteoclastic RAW 264.7 Cells by Inhibiting the RANK/NFκB/cFOS/NFATc1 Signalling PathwayMoleculesMay 2, 2024Purithat Rattajak, Aratee Aroonkesorn, Carl Smythe, Rapepun Wititsuwannakul, Thanawat PitakpornpreechaSanta Cruz Biotechnology) for 3 h and counterstained with 4′,6-diamidino-2-phenylindole (DAPI) (No. 4083, Cell Signaling Technology, Danvers, MA, USA) for 5 min. Finally, mounting medium was carefully added

(151) Histone H3K9 Lactylation Confers Temozolomide Resistance in Glioblastoma via LUC7L2‐Mediated MLH1 Intron RetentionAdvanced ScienceMay 1, 2024Qu Yue, Zhao Wang, Yixiong Shen, Yufei Lan, Xiangyang Zhong, Xin Luo, Tao Yang, Manqing Zhang, Boming Zuo, Tianci Zeng, et al.secondary antibodies. Nuclear counterstaining was carried out with DAPI (Cell Signaling Technology, #4083, USA). Phaloidin was used to stain intracellular actin (Cell Signaling Technology, #13 054, USA). The

(152) Mitophagy Regulates the Circadian Rhythms by Degrading NR1D1 in Simulated Microgravity and Isolation EnvironmentsInternational Journal of Molecular SciencesApril 29, 2024Sihai Zhou, Xiaopeng Li, Fengji Liang, Guohua Ji, Ke Lv, Yanhong Yuan, Yujie Zhao, Na Yan, Chuanjie Zhang, Shiou Cai, et al.another three washes with PBS, the cells were incubated with DAPI (1:4000; cell signaling Technology; 4083, Danvers, MA, USA) in PBS for 5 min, followed by a final wash with PBS. Cell images were acquired using

(153) Cultivation, cryopreservation, and transcriptomic studies of host-adapted Cryptosporidium parvum and Cryptosporidium hominis using enteroidsiScienceApril 19, 2024Miner Deng, Tianyi Hou, Jie Zhang, Xinjie Mao, Fuxian Yang, Yanting Wei, Yongping Tang, Wanting Zeng, Wanyi Huang, Na Li, et al.CS10,STEMCELL,Cat#07930 BSA,Biofroxx,Cat#4240GR100 4% paraformaldehyde,Leagene,Cat#DF0135 Triton X-100,Sigma,Cat#9002-93-1 DAPI,CST,Cat# 4083S Advanced DMEM/F-12,Gibco,Cat#12634-010 DMEM,Gibco,Cat#c11995500bt DMEM/F-12,Gibco,Cat#11320033 ,, Critical

(154) Identification of Novel FZD4 Mutations in Familial Exudative Vitreoretinopathy and Investigating the Pathogenic Mechanisms of FZD4 MutationsInvestigative Opthalmology & Visual ScienceApril 1, 2024Erkuan Dai, Min Liu, Shujin Li, Xiang Zhang, Shiyuan Wang, Rulian Zhao, Yunqi He, Li Peng, Liting Lv, Haodong Xiao, et al.washed with PBS three times and then incubated with fluorescently labeled secondary antibodies and DAPI (4083; Cell Signaling Technology, Danvers, MA, USA) for one hour at room temperature. Fluorescence was observed

(155) Amyloid β oligomer induces cerebral vasculopathy via pericyte-mediated endothelial dysfunctionAlzheimer's research & therapyMarch 12, 2024Chen S, Guo D, Zhu Y, Xiao S, Xie J, Zhang Z, Hu Y, Huang J, Ma X, Ning Z, et al.Appropriate secondary antibodies (1:1000, Jackson, USA), 4,6-diamidino-2-phenylindole (DAPI, 1:1000, CST, 4083S, USA) and Lectin with DyLight™ 649 Lycopersicon Esculentum (Tomato) (Vector, DL-1178–1) were used for

(156) Investigating the Impact of Dimer Interface Mutations on Norrin's Secretion and Norrin/β-Catenin Pathway ActivationInvestigative ophthalmology & visual scienceMarch 5, 2024Liu M, Dai E, Yang M, Li S, Fan L, Liu Y, Xiao H, Zhao P, Yang Z.coverslips were washed with PBS 3 times and incubated with fluorescent secondary antibodies and DAPI (4083; CST) for 1 hour at room temperature. Fluorescence was visualized by LSM900 confocal microscope (Zeiss)

(157) Hapln1 promotes dedifferentiation and proliferation of iPSC-derived cardiomyocytes by promoting versican-based GDF11 trappingJournal of pharmaceutical analysisMarch 1, 2024Hao DJ, Qin Y, Zhou SJ, Dong BH, Yang JS, Zou P, Wang LP, Zhao YT.antibodies. The cells were then counterstained with 0.5 μg/mL of 4′,6-diamidino-2-phenylindole (DAPI) (4083; Cell Signaling Technology) for 15 min at room temperature. After being washed with phosphate-buffered

(158) A mRNA Vaccine for Crimean–Congo Hemorrhagic Fever Virus Expressing Non-Fusion GnGc Using NSm Linker Elicits Unexpected Immune Responses in MiceVirusesFebruary 28, 2024Tong Chen, Zhe Ding, Xuejie Li, Yingwen Li, Jiaming Lan, Gary WongChina), diluted 1:1000 with PBS, were incubated for 1 h in darkness. After three washes with PBS, DAPI (4083, Cell Signaling Technology), diluted 1:10,000 with PBS, was incubated for 8 min in darkness. After two

(159) Tumor-derived extracellular vesicle drug delivery system for chemo-photothermal-immune combination cancer treatmentiScienceFebruary 16, 2024Yanghui Bi, Jieya Chen, Qing Li, Yan Li, Ling Zhang, Liu Zhida, Fajia Yuan, Ruiping Zhangproteins" ,, Albumin-bound paclitaxel,Hunan Kelun Pharmaceutical,H20203443 DAPI,Cell Signaling Technology,Cat#4083S Dil,Solarbio,Cat#D8700-10mg Dir,Solarbio,Cat#IC6110 Amiloride HCl dihydrate,Solarbio,Cat#A0080 Chlorpromazine

(160) Reduced miR‐513a‐5p expression in COPD may regulate airway mucous cell hyperplasia through TFR1‐dependent signalingThe Kaohsiung Journal of Medical SciencesFebruary 1, 2024Jia Zhou, Jun‐Yi Du, Rui Xu, Xiao‐Juan Wu, Guo‐Yue ZhangMA) at a 1:500 dilution. After washing with PBS, the cell nuclei were stained with 0.5 μg/mL DAPI (#4083, Cell Signaling, Danvers, MA). After washing three times, the slides were embedded in 50% glycerol.

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DAPI

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