DAPI from Cell Signaling Technology

Product Specs
ItemDAPI
CompanyCell Signaling Technology
Price $74.00 Inquire
Catalog Number4083S
Quantity1 mg
Research AreaRelated Products
ApplicationsIF-IC, FC-FP

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

Website: Technical Support

(201) Lineage-specific regulatory changes in hypertrophic cardiomyopathy unraveled by single-nucleus RNA-seq and spatial transcriptomicsCell DiscoveryJanuary 17, 2023Xuanyu Liu, Kunlun Yin, Liang Chen, Wen Chen, Wenke Li, Taojun Zhang, Yang Sun, Meng Yuan, Hongyue Wang, Yunhu Song, et al.stained with primary antibodies targeting Collagen-I (ab138492, Abcam). Nuclei were stained with DAPI (4083S, Cell Signaling Technology). Fluorescence intensity was analyzed by ImageJ. siRNA transfection Human

(202) Effect of Hypoxia-Ischemia on the Expression of Iron-Related Proteins in Neonatal Rat BrainsNeural plasticityJanuary 1, 2023Lin Q, Hu DW, Hao XH, Zhang G, Lin L.ab150117) for 1 hour at room temperature. Sections were subsequently washed in PBST and DAPI (CST, 4083). The results were visualized and photographed under a confocal laser-scanning microscope (Leica, TCS

(203) Sex and diet-dependent gene alterations in human and rat brains with a history of nicotine exposureFrontiers in psychiatryJanuary 1, 2023Vargas-Medrano J, Carcoba LM, Vidal Martinez G, Mulla ZD, Diaz V, Ruiz-Velasco A, Alvarez-Primo F, Colina G, Iñiguez SD, Thompson PM, et al.4°C. Finally, the slides were washed twice with wash buffer. Cell nuclei were stained with DAPI (Cat# 4083S, Cell Signaling Technology Inc., Danvers, MA, USA), and mounted with Fluromount-G slide mounting medium

(204) miR‑146a‑5p protects against renal injury in MRL/lpr mice via improvement of the Treg/Th17 imbalance by targeting the TRAF6/NF‑κB axisExperimental and therapeutic medicineJanuary 1, 2023Teng J, Yang F, Li X.ab96899; Abcam) secondary antibodies, samples were incubated at room temperature for 2 h. DAPI (cat. no. 4083, Cell Signaling Technology, Inc) was used as a nuclear counterstain and samples were incubated at room

(205) Nucleoporin POM121 signals TFEB-mediated autophagy via activation of SIGMAR1/sigma-1 receptor chaperone by pridopidineAutophagyJanuary 1, 2023Wang SM, Wu HE, Yasui Y, Geva M, Hayden M, Maurice T, Cozzolino M, Su TP.times with PBS and mounted with Prolong gold antifade mountant with DAPI (Cell Signaling Technology, 4083S). Images of cells were captured by confocal microscopy (Perkin-Elmer Modular laser system 2.0 with Nikon

(206) Kir4.1 channel activation in NG2 glia contributes to remyelination in ischemic strokeEBioMedicineJanuary 1, 2023Hong X, Jian Y, Ding S, Zhou J, Zheng X, Zhang H, Zhou B, Zhuang C, Wan J, Tong X.secondary antibodies for 2 h at room temperature. Sections were incubated with DAPI (1:1000, Cell Signaling 4083S) for 15 min. The primary antibodies included Chicken antibody to GFP (Abcam Cat# ab13970, RRID:AB_300798),

(207) Inhibition of the LRRC8A channel promotes microglia/macrophage phagocytosis and improves outcomes after intracerebral hemorrhagic strokeiScienceDecember 22, 2022Liu J, Shen D, Wei C, Wu W, Luo Z, Hu L, Xiao Z, Hu T, Sun Q, Wang X, et al.TRIzol reagent,Invitrogen,15596018 DMEM/F12,Gibco,11039021 Penicillin/streptomycin,Sigma-Aldrich,V900929 DAPI,Cell Signaling Technology,4083S DCPIB,Tocris,1540/10 Hemin,Frontier Scientific,7084–24–4 ML385,Selleck,S8790 RIPA Lysis buffer,Boster,AR0101 30%

(208) Alpinetin Suppresses Zika Virus-Induced Interleukin-1β Production and Secretion in Human MacrophagesPharmaceuticsDecember 14, 2022Wikan N, Potikanond S, Hankittichai P, Thaklaewphan P, Monkaew S, Smith DR, Nimlamool W.secondary antibody (1:400), 1 μg/mL of 4′,6-diamidino-2-phenylindole, dihydrochloride (DAPI) (Cat No. 4083) (Cell Signaling Technology, Danvers, MA, USA) for nuclei staining, and with 1× of DyLight TM 594-Phalloidin

(209) Hepatic PLIN5 Deficiency Impairs Lipogenesis through Mitochondrial DysfunctionInternational journal of molecular sciencesDecember 9, 2022Zhang E.imaging. Nuclei were stained with DAPI (4′, 6-diamidino-2-phenylindole) (Cell Signaling, Danvers, MA, USA, 4083S) for 10 min followed by mounting onto slides for visualization. Images from 6 different fields per well

(210) Maternal rodent exposure to di-(2-ethylhexyl) phthalate decreases muscle mass in the offspring by increasing myostatinJournal of cachexia, sarcopenia and muscleDecember 1, 2022Li F, Luo T, Rong H, Lu L, Zhang L, Zheng C, Yi D, Peng Y, Lei E, Xiong X, et al.dilution, Invitrogen) 1 h at RT, followed by nuclear staining with 4′6‐diamidino‐2‐phenylindole (cat# 4083, CST). The TA muscle sections (5 μm) were stained with anti‐laminin antibody (cat# L0663, Sigma) for

(211) The NALCN channel regulates metastasis and nonmalignant cell disseminationNature geneticsDecember 1, 2022Rahrmann EP, Shorthouse D, Jassim A, Hu LP, Ortiz M, Mahler-Araujo B, Vogel P, Paez-Ribes M, Fatemi A, Hannon GJ, et al.Thermo Fisher Scientific, 1:500). Sections were counterstained (4,6-diamidino-2-phenylindole (DAPI); catalog number 4083, Cell Signaling, 1:10,000) and images captured using a Zeiss ImagerM2 and Apotome microscope or Zeiss

(212) Anti-TGF-β/PD-L1 bispecific antibody promotes T cell infiltration and exhibits enhanced antitumor activity in triple-negative breast cancerJournal for immunotherapy of cancerDecember 1, 2022Yi M, Wu Y, Niu M, Zhu S, Zhang J, Yan Y, Zhou P, Dai Z, Wu K.1:300) was incubated at room temperature for 1 hour. The cell nuclei were counterstained with DAPI (4083, CST, 1 µg/mL). Western blotting Western blotting was performed as previously described.26 The following

(213) Chemically defined and small molecules-based generation of sinoatrial node-like cellsStem Cell Research & TherapyDecember 1, 2022Xiaojie Hou et al.Additional file 2: Table S2. Wash with PBS three times before each step. Nuclei were stained with DAPI (4083, Cell Signaling Technology, USA) for 15 min at room temperature. Fluorescence images were captured by

(214) Albumin alleviated esketamine-induced neuronal apoptosis of rat retina through downregulation of Zn2+-dependent matrix metalloproteinase 9 during the early developmentBMC NeuroscienceNovember 16, 2022Kan Zhang, Ruijing Ma, Luping Feng, Peiwen Liu, Shuang Cai, Chaoyang Tong, Jijian ZhengAlexa Fluor 488 (1:500, Abcam ab150109). Sections were incubated with DAPI (1:1000, Cell Signaling 4083 S) for 15 min. Fluorescent images were visualized using a confocal microscope (Leica TCS SP8). Images

(215) PPAR agonists attenuate lenalidomide's anti-myeloma activity in vitro and in vivoCancer lettersOctober 1, 2022Sha Y, Wu J, Paul B, Zhao Y, Mathews P, Li Z, Norris J, Wang E, McDonnell DP, Kang Y.thrice with PBS, the slides were stained with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI; 4083; Cell Signaling) for 5 min and mounted with the antifade mounting medium (Vector, H-1000). Images were

(216) FBXO42 facilitates Notch signaling activation and global chromatin relaxation by promoting K63-linked polyubiquitination of RBPJScience AdvancesSeptember 23, 2022Hua Jiang, Weixiang Bian, Yue Sui, Huanle Li, Han Zhao, Wenqi Wang, Xu LiRRID:AB_2630356) at room temperature for 1 hour, and subjected to 4′,6-diamidino-2-phenylindole (DAPI) staining (4083S, CST). Coverslips were mounted using FluorSave reagent (345789, Millipore). The cells were viewed using

(217) Targeting the succinate receptor effectively inhibits periodontitisCell reportsSeptember 20, 2022Guo Y, Xu F, Thomas SC, Zhang Y, Paul B, Sakilam S, Chae S, Li P, Almeter C, Kamer AR, et al./14190-136 Succinate,Sigma,14160-100G EDTA,Fisher Chemical,E478-1 BlockAid™ Blocking Solution,Invitrogen,B10710 DAPI,Cell Signaling Technology,4083 ActinGreen™ 488,Invitrogen,R37110 Sodium acetate,Sigma,S2889 L-(+) Tartaric acid,Sigma,T6521 Glacial

(218) A positive mechanobiological feedback loop controls bistable switching of cardiac fibroblast phenotypeCell discoverySeptember 6, 2022Niu L, Cheng B, Huang G, Nan K, Han S, Ren H, Liu N, Li Y, Genin GM, Xu F.by Rhodamine phalloidin (1:500; Invitrogen, R415, USA). DAPI (0.5 μg/mL; Cell Signaling Technology, 4083, USA) was used to stain cell nuclei. Immunofluorescent staining for YAP/F-actin/DAPI was used to calculate

(219) AKR1C1 Protects Corneal Epithelial Cells Against Oxidative Stress-Mediated Ferroptosis in Dry EyeInvestigative Opthalmology & Visual ScienceSeptember 6, 2022Xin Zuo, Hao Zeng, Bowen Wang, Xue Yang, Dalian He, Li Wang, Hong Ouyang, Jin Yuananti–rabbit IgG (#4412), Alexa Fluor 488-labeled donkey anti-mouse IgG (#4409) antibodies, and DAPI (#4083) were purchased from Cell Signaling Technology (Danvers, MA, USA). The following antibodies were purchased

(220) The Biphasic Effect of Retinoic Acid Signaling Pathway on the Biased Differentiation of Atrial-like and Sinoatrial Node-like Cells from hiPSCInternational journal of stem cellsAugust 30, 2022Liu F, Long D, Huang W, Peng W, Lan H, Zhou Y, Dang X, Zhou R.Invitrogen, USA). The cells were washed 3 times with PBS and then counterstained using 0.5 μg/ml of DAPI (4083, Cell Signaling Technology, USA) for 15 min at RT. The chambers were mounted and visualized under fluorescence microscopy

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DAPI

DAPI from Cell Signaling Technology

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