DAPI from Cell Signaling Technology

Product Specs
ItemDAPI
CompanyCell Signaling Technology
Price $74.00 Inquire
Catalog Number4083S
Quantity1 mg
Research AreaRelated Products
ApplicationsIF-IC, FC-FP

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(101) A Potent, Selective, Small-Molecule Inhibitor of DHX9 Abrogates Proliferation of Microsatellite Instable Cancers with Deficient Mismatch RepairCancer researchFebruary 17, 2025Castro J, Daniels MH, Brennan D, Johnston B, Gotur D, Lee YT, Knockenhauer KE, Lu C, Wu J, Nayak S, et al.Alexa Fluor 555 phalloidin (8953, Cell Signaling Technology) or 4′,6-diamidino-2-phenylindole (DAPI; 4083, Cell Signaling Technology) and imaged using a Nikon Eclipse Ti2 system. G-quadruplexes LS411N MSI-H/dMMR

(102) Klotho protects INS-1 pancreatic β-cells from senescence and enhances mitochondrial functionFront AgingFebruary 13, 2025Zhihong Wang, Yunzhi Ni, Yan-Ru Lou, Gerald J. Prud'homme, Qinghua Wangfollowed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488 (1:1000; ab150077, Abcam) and DAPI (1 μg/mL, 4083S, Cell Signaling Technology). All immunofluorescent images were captured by an Olympus upright BX50 fluorescence microscope

(103) NF2 is Essential for Human Endoderm DevelopmentAdvanced ScienceFebruary 8, 2025Minjin Jeong, Dongjun Han, Preetida Bhetariya, D. Bradley Welling, Miodrag Stojkovic, Konstantina M. StankovicSupplementary Table) incubations. Vectashield (Vector Laboratories, #H‐1000‐10) with 1x DAPI (Cell Signaling, #4083S) was used to mount the samples and visualize cellular nuclei. Negative control experiments without the

(104) PGC-1α activation to enhance macrophage immune function in mycobacterial infectionsPLoS OneFebruary 6, 2025Joel R. Frandsen, Zhihong Yuan, Brahmchetna Bedi, Zohra Prasla, Seoung-Ryoung Choi, Prabagaran Narayanasamy, Ruxana T. SadikotT668), MitoSOX Red (Invitrogen, M36007) 4’, 6-diamidino-2-phenylindole, dihydrochloride (DAPI) (CST, 4083), Hoechst 33342 (CST, 4082). Unless otherwise specified, reagents and chemicals were obtained through

(105) Brd7 loss reawakens dormant metastasis initiating cells in lung by forging an immunosuppressive nicheNature communicationsFebruary 5, 2025Mondal J, Zhang J, Qing F, Li S, Kumar D, Huse JT, Giancotti FG.PBS and incubated with appropriate secondary antibodies for 1 h at room temperature. DAPI (1:1000; 4083, Cell Signaling Technology) was used to mark the nuclei, and fluorescence images were captured by a

(106) Targeting pseudoknots with Cas13b inhibits porcine epidemic diarrhoea virus replicationJ Gen VirolFebruary 4, 2025Hee-Jeong Han, Daseuli Yu, Jeonghye Yu, Jihye Kim, Won Do Heo, Dongseob Tark, Sang-Min Kangfragment conjugated with Alexa Fluor 488, Cell Signaling Technology). Nuclei were stained with DAPI (no. 4083S; Cell Signaling Technology), and fluorescent images were captured using the CELENA X digital imaging

(107) TNBC response to paclitaxel phenocopies interferon response which reveals cell cycle-associated resistance mechanismsSci RepFebruary 4, 2025Nicholas L. Calistri, Tiera A. Liby, Zhi Hu, Hongmei Zhang, Mark A. Dane, Sean M. Gross, Laura M. HeiserAbcam) overnight at 4C. Each well was washed twice with room temp PBS then stained with 0.5ug/mL DAPI (4083S, Cell Signaling Technology) in PBS for 15 min at room temperature. Following DAPI staining, wells were

(108) Halodule uninervis extract facilitates the green synthesis of gold nanoparticles with anticancer activitySci RepFebruary 4, 2025Nadine Wehbe, Joelle Edward Mesmar, Riham El Kurdi, Ali Al-Sawalmih, Adnan Badran, Digambara Patra, Elias Baydoun×20, and ×40 magnifications. 4′, 6-diamidino-2-phenylindole, dihydrochloride (DAPI) (Cell signaling #4083) staining was used to determine changes in nuclear morphology. Cells were grown in 12-well plates in

(109) Neuronal cathepsin S increases neuroinflammation and causes cognitive decline via CX3CL1-CX3CR1 axis and JAK2-STAT3 pathway in aging and Alzheimer's diseaseAging cellFebruary 1, 2025Liu PP, Liu XH, Ren MJ, Liu XT, Shi XQ, Li ML, Li SA, Yang Y, Wang DD, Wu Y, et al.antibodies (Invitrogen, Carlsbad, California, USA). 4′, 6‐Diamidino‐2‐phenylindole (DAPI) (1:106, CST, #4083) was used to stain the nuclei. 2.7 Image acquisition and analysis The stained specimens were imaged

(110) c-Rel drives pancreatic cancer metastasis through Fibronectin-Integrin signaling-induced isolation stress resistance and EMT activationbioRxiv : the preprint server for biologyFebruary 1, 2025Bakırdöğen D, Görgülü K, Xin J, Alcalá S, Ruiz-Cañas L, Frank K, Wu N, Diakopoulos KN, Dai C, Öztürk H, et al.PBS with 1:5000 Zombie-Red (BioLegend 423110), or 300μL FC buffer with 1:5000 DAPI (Cell Signaling, 4083S), depending on the panel. Before analyses, suspensions are passed through a 35μm strainer (Corning 352235).

(111) Innovative treatment of age-related hearing loss using MSCs and EVs with ApelinCell biology and toxicologyJanuary 17, 2025Xu S, Liu D, Zhang F, Tian Y.thorough wash with PBS before being affixed using an anti-fade mounting compound infused with DAPI (4083, CST). Subsequent observations and image capture were performed utilizing a laser scanning confocal

(112) Bacteria in hypertrophic scars promote scar formation through HSBP1-mediated autophagyWound Repair RegenJanuary 17, 2025Bo Yuan, Jiarong Yu, Jiaoyun Dong, Zhigang Mao, Xiqiao Wangincubated with 4′,6‐diamidino‐2‐phenylindole (DAPI, 1:1000, Cell Signalling Technology, catalogue number 4083) for an appropriate duration. Following three additional washes, the slides were mounted and observed

(113) AAVR Expression is Essential for AAV Vector Transduction in Sensory Hair CellsAdvanced ScienceJanuary 7, 2025Fan Wu, Guisheng Chen, Rui Hu, Peiwen Liu, Jintao Lou, Wenji Zhao, Zuhong He, Suhua Sha, Yiqing Zheng1:200 at 4 °C overnight. Nuclei were counterstained with DAPI (1:1000, Cell Signaling Technology, #4083) at room temperature for 10 min. Immunolabeled images were taken with a Zeiss LSM 880. Immuno‐Colocalization

(114) Bioengineered miR-7-5p modulates non–small cell lung cancer cell metabolism to improve therapyMolecular PharmacologyJanuary 1, 2025Gavin M. Traber, Mei-Juan Tu, Su Guan, Neelu Batra, Ai-Ming YuCat# A22286) and with 4ʹ,6-diamidino-2-phenylindole (DAPI; 1 μg/mL; Cell signaling Technology Cat# 4083S) nuclear stain prior to mounting with VECTASHIELD Antifade (20 μL/chamber slide well; Fisher Scientific

(115) In vivo hyperphosphorylation of tau is associated with synaptic loss and behavioral abnormalities in the absence of tau seedsNature NeuroscienceDecember 24, 2024Naoto Watamura, Martha S. Foiani, Sumi Bez, Mathieu Bourdenx, Alessia Santambrogio, Claire Frodsham, Elena Camporesi, Gunnar Brinkmalm, Henrik Zetterberg, Saisha Patel, et al.Finally, sections were stained with 4,6-diamidino-2-phenylindole (Cell Signaling Technology, cat. no. 4083S) diluted in TNB buffer before mounting with PermaFluor (Thermo Fisher Scientific, cat. no. TA-030-FM).

(116) BPDCN MYB fusions regulate cell cycle genes, impair differentiation, and induce myeloid-dendritic cell leukemiaJCI insightDecember 20, 2024Booth CAG, Bouyssou JM, Togami K, Armand O, Rivas HG, Yan K, Rice S, Cheng S, Lachtara EM, Bourquin JP, et al.washed twice in PBS + 2% FBS and then resuspended in PBS + 1 μg/mL DAPI (Cell Signaling Technology, 4083) + 0.1 μg/mL RNase A (Thermo Fisher Scientific, EN0531) and incubated for 1 hour at 22°C before flow cytometry analysis

(117) Protocol for isolating CD163+ Kupffer cells from human liver resectionsSTAR ProtocolsDecember 20, 2024Armando Andres Roca Suarez, Marie-Laure Plissonnier, Maud Michelet, Anaëlle Dubois, Sarah Heintz, Maria Saez-Palma, Marion Delphin, Isabelle Bordes, Jennifer Molle, Audrey Diederichs, et al.serum,Cytiva,SH30066.03 OptiPrep density gradient medium,BioVision,M1248-250 DAPI,Cell Signaling Technology,4083 Paraformaldehyde (PFA) 16% solution,Electron Microscopy Sciences,15710 Dako fluorescent mounting medium,Dako,S3023 Selgantolimod

(118) Optogenetically Induced Microtubule Acetylation Unveils the Molecular Dynamics of Actin-Microtubule Crosstalk in Directed Cell MigrationbioRxivDecember 8, 2024Abhijit Deb Deb Roy, Cristian Saez Saez Gonzalez, Farid Shahid, Eesha Yadav, Takanari Inoueincubated with secondary antibody, Phalloidin (ThermoFisher A22286) and DAPI (Cell Signaling Technology, 4083S). After that, they were washed three times in PBS and the images were captured by microscopy. For HeLa cells

(119) An In Vitro Oxidative Stress Model of the Human Inner Ear Using Human-Induced Pluripotent Stem Cell-Derived Otic Progenitor CellsAntioxidants (Basel)November 16, 2024Minjin Jeong, Sho Kurihara, Konstantina M. Stankovic1:1000). The nuclei were stained with 0.1 μg/mL DAPI (Cell Signaling Technology, Danvers, MA, USA, #4083) and Vectashield (Vector Laboratories, Burlingame, CA, USA, # H1000) was used to mount the samples.

(120) Inhibition of the Notch signal transducer CSL by Pkc53E-mediated phosphorylation to fend off parasitic immune challenge in DrosophilaeLifeNovember 6, 2024Deichsel S, Frankenreiter L, Fechner J, Gahr BM, Zimmermann M, Mastel H, Preis I, Preiss A, Nagel AC.or kit,Pap-pen,Kisker Biotech,Cat# MKP-1, "Chemical compound, drug",DAPI,Cell Signaling Techn.,Cat# 4083,(1 µg/ml) "Chemical compound, drug",DNase I,New England Biolabs GmbH,Cat# M0303,(2 U/µl) "Chemical

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DAPI

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