DAPI from Cell Signaling Technology

Product Specs
ItemDAPI
CompanyCell Signaling Technology
Price $74.00 Inquire
Catalog Number4083S
Quantity1 mg
Research AreaRelated Products
ApplicationsIF-IC, FC-FP

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(101) CRISPR-Cas9 genetic screens reveal regulation of TMPRSS2 by the Elongin BC-VHL complexSci RepApril 7, 2025Ildar Gabaev, Alexandra Rowland, Emilija Jovanovic, Christian M. Gawden-Bone, Thomas W. M. Crozier, Ana Teixeira-Silva, Edward J. D. Greenwood, Pehuén Pereyra Gerber, Niek Wit, James A. Nathan, et al.with secondary antibody for 30 min. Cell viability was assessed using DAPI (Cell Signaling, Cat. No. 4083S) or Zombie green Fixable Viability kit (Biolegend, Cat. No. 423112). Measurements were performed on

(102) Subtle behavioral alterations in the spontaneous behaviors of MPTP mouse model of Parkinson's diseaseTransl PsychiatryApril 3, 2025Hao Zhong, Kangrong Lu, Liping Wang, Wanshan Wang, Pengfei Wei, Xuemei LiuAlexa Fluor 488 (A28175, ThermoFisher; 1:500 in PBS buffer) for 1 h and DAPI (1:5000 in PBS buffer, 4083S, Cell Signaling Technology, USA) for 5 min in the absence of light at room temperature. Finally, the

(103) A novel senotherapeutic strategy with azithromycin for preventing endometriosis progressionReprod Biol EndocrinolMarch 26, 2025Reina Sonehara, Tomoko Nakamura, Takehiko Takeda, Satoshi Kaseki, Tomomi Seki, Hideaki Tanaka, Atsushi Yabuki, Natsuki Miyake, Ayako Muraoka, Satoko Osuka, et al.antibodies for 1 h at room temperature. Nuclear staining was performed using 4’,6-diamidino2-phenylindole (4083, 1:1000; Cell Signaling Technology, Danvers, MA, USA). Visualization was performed using a confocal

(104) Potentiating anti-tumor immunity by re-engaging immune synapse moleculesCell Reports MedicineMarch 18, 2025Xindi Zhou, Tian Xu, Changhe Li, Yufeng He, Yuanzhi Hu, Hao Gong, Jiahui Li, Haitao Jiang, Liang Wen, Yangxin Fu, et al.,Cell Signaling Technology,Cat# 9803S ACK Lysis Buffer,LEAGENE,Cat# CS0001 DAPI,Cell Signaling Technology,Cat# 4083 Fixable Viability Dye eFluor™ 506,Thermo Fisher Scientific,Cat# 65-0866-18 "Brefeldin A Solution (1,000X)",Biolegend,Cat#

(105) Exosome-mediated effects of BRCA1 on cardiovascular artery diseaseCell Biol ToxicolMarch 13, 2025Hairui Yu, Dong Wei, Weiqian Liao, Xiaoming Shang, Dandan Li, Chunzhao Liu, Qimei Deng, Haiquan Huangfuthe labeled Exo was fixed in 4% paraformaldehyde and mounted with an antifade medium containing DAPI (4083, CST). Imaging and observation were conducted using a laser scanning confocal microscope (LSM 980, ZEISS).

(106) Exploring vestibulocerebellum-vestibular nuclei-spinal trigeminal nucleus causals communication and TRPV2 ion channel in a mouse model of vestibular migraineJ Headache PainMarch 5, 2025Qingling Zhai, Qihui Chen, Ning Zhang, Hongyan Li, Qijun Yu, Yonghui PanNumber,Dilution CGRP,CST,"14,959",0.388889 c-Fos,CST,2250,0.736111 Neuron,Abcam,EPR2763,0.736111 DAPI,CST,4083 S,2.125 TRPV2,Abcam,ab272862,0.388889 IBA1,Proteintech,26177-1-AP,0.388889 NLRP3,Proteintech,19771-1-AP,0.73611 ASC,Bioss,bs-6741R,0.38889 cleaved

(107) Targeting Caveolin-1 for enhanced rotator cuff repair: findings from single-cell RNA sequencingCell death discoveryMarch 5, 2025Fang S, Wu S, Chen P.by three washes with PBS. Finally, the slides were mounted using a mounting medium containing DAPI (4083, CST) for fluorescence quenching [55]. For EdU staining, 3T3-L1 cells were seeded in a 24-well plate

(108) Artemisia annua L. leaf extracts suppress influenza virus infection by targeting the viral nucleoprotein and blocking mitochondria-mediated apoptosisVirologica SinicaMarch 3, 2025Xiwen Zhao, Xuan Dai, Fuyi Wang, Chenyang Li, Xun Song, Yingying Han, Chaowei Zhang, Lu Wang, Zhendan He, Rongping Zhang, et al.containing 0.3% Triton X-100. After staining the cells with 1 μg/mL DAPI (Cell Signaling Technology, 4083) for 20 min, they were observed under a confocal microscope and analyzed using ZEISS ZEN digital microscope

(109) The levels of the long non-coding RNA MALAT1 affect cell viability and modulate TDP-43 binding to mRNA in the nucleusJournal of Biological ChemistryMarch 1, 2025Adarsh Balaji, Aileen C. Button, Simone D. Hall, Jonathan Zhu, Lauren Ellis, Ellen Lavorando, Ethan L. Ashley, Raul Johnson, Einollah Sarikhani, Zeinab Jahed, et al.three washes with 1× PBS for 5 min. For visualization, cells were incubated in 1 μg/ml DAPI DNA stain (4083S, Cell Signaling Technology) for 1 min, rinsed with 1× PBS, and imaged using an Eclipse Ti Inverted Microscope

(110) Dietary nitrate supplementation mitigates age‐related changes at the neuromuscular junction in miceJ PhysiolFebruary 26, 2025Maira Rossi, Lucrezia Zuccarelli, Lorenza Brocca, Cristiana Sazzi, Clarissa Gissi, Paola Rossi, Bruno Grassi, Simone Porcelli, Roberto Bottinelli, Maria Antonietta Pellegrinocounterstained for 5 min with 4′,6‐diamidino‐2‐phenylindole (DAPI) (Cell Signaling Technology; catalog. no. #4083) diluted 1:5000 in PBS. Negative controls (without the primary antibody incubation) were performed in

(111) Rebastinib inhibits FoxO1 activity and reduces dexamethasone-induced atrophy and its-related gene expression in cultured myotubesJ Physiol SciFebruary 18, 2025Tomoki Sato, Akihito Morita, Yui Watanabe, Yumi Naito, Haruka Kawaji, Takumi Nakagawa, Hiroki Hamaguchi, Yasuko Manabe, Nobuharu L. Fujii, Naohisa Ogo, et al.568 secondary antibody (A21144; Invitrogen), for 1 h followed by nuclear counterstaining with DAPI (#4083; Cell Signaling Technology). Myotubes images were captured using a fluorescence microscope (Leica Microsystems,

(112) A Potent, Selective, Small-Molecule Inhibitor of DHX9 Abrogates Proliferation of Microsatellite Instable Cancers with Deficient Mismatch RepairCancer researchFebruary 17, 2025Castro J, Daniels MH, Brennan D, Johnston B, Gotur D, Lee YT, Knockenhauer KE, Lu C, Wu J, Nayak S, et al.Alexa Fluor 555 phalloidin (8953, Cell Signaling Technology) or 4′,6-diamidino-2-phenylindole (DAPI; 4083, Cell Signaling Technology) and imaged using a Nikon Eclipse Ti2 system. G-quadruplexes LS411N MSI-H/dMMR

(113) Klotho protects INS-1 pancreatic β-cells from senescence and enhances mitochondrial functionFront AgingFebruary 13, 2025Zhihong Wang, Yunzhi Ni, Yan-Ru Lou, Gerald J. Prud'homme, Qinghua Wangfollowed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488 (1:1000; ab150077, Abcam) and DAPI (1 μg/mL, 4083S, Cell Signaling Technology). All immunofluorescent images were captured by an Olympus upright BX50 fluorescence microscope

(114) NF2 is Essential for Human Endoderm DevelopmentAdvanced ScienceFebruary 8, 2025Minjin Jeong, Dongjun Han, Preetida Bhetariya, D. Bradley Welling, Miodrag Stojkovic, Konstantina M. StankovicSupplementary Table) incubations. Vectashield (Vector Laboratories, #H‐1000‐10) with 1x DAPI (Cell Signaling, #4083S) was used to mount the samples and visualize cellular nuclei. Negative control experiments without the

(115) PGC-1α activation to enhance macrophage immune function in mycobacterial infectionsPLoS OneFebruary 6, 2025Joel R. Frandsen, Zhihong Yuan, Brahmchetna Bedi, Zohra Prasla, Seoung-Ryoung Choi, Prabagaran Narayanasamy, Ruxana T. SadikotT668), MitoSOX Red (Invitrogen, M36007) 4’, 6-diamidino-2-phenylindole, dihydrochloride (DAPI) (CST, 4083), Hoechst 33342 (CST, 4082). Unless otherwise specified, reagents and chemicals were obtained through

(116) Brd7 loss reawakens dormant metastasis initiating cells in lung by forging an immunosuppressive nicheNature communicationsFebruary 5, 2025Mondal J, Zhang J, Qing F, Li S, Kumar D, Huse JT, Giancotti FG.PBS and incubated with appropriate secondary antibodies for 1 h at room temperature. DAPI (1:1000; 4083, Cell Signaling Technology) was used to mark the nuclei, and fluorescence images were captured by a

(117) TNBC response to paclitaxel phenocopies interferon response which reveals cell cycle-associated resistance mechanismsSci RepFebruary 4, 2025Nicholas L. Calistri, Tiera A. Liby, Zhi Hu, Hongmei Zhang, Mark A. Dane, Sean M. Gross, Laura M. HeiserAbcam) overnight at 4C. Each well was washed twice with room temp PBS then stained with 0.5ug/mL DAPI (4083S, Cell Signaling Technology) in PBS for 15 min at room temperature. Following DAPI staining, wells were

(118) Halodule uninervis extract facilitates the green synthesis of gold nanoparticles with anticancer activitySci RepFebruary 4, 2025Nadine Wehbe, Joelle Edward Mesmar, Riham El Kurdi, Ali Al-Sawalmih, Adnan Badran, Digambara Patra, Elias Baydoun×20, and ×40 magnifications. 4′, 6-diamidino-2-phenylindole, dihydrochloride (DAPI) (Cell signaling #4083) staining was used to determine changes in nuclear morphology. Cells were grown in 12-well plates in

(119) Targeting pseudoknots with Cas13b inhibits porcine epidemic diarrhoea virus replicationJ Gen VirolFebruary 4, 2025Hee-Jeong Han, Daseuli Yu, Jeonghye Yu, Jihye Kim, Won Do Heo, Dongseob Tark, Sang-Min Kangfragment conjugated with Alexa Fluor 488, Cell Signaling Technology). Nuclei were stained with DAPI (no. 4083S; Cell Signaling Technology), and fluorescent images were captured using the CELENA X digital imaging

(120) Neuronal cathepsin S increases neuroinflammation and causes cognitive decline via CX3CL1-CX3CR1 axis and JAK2-STAT3 pathway in aging and Alzheimer's diseaseAging cellFebruary 1, 2025Liu PP, Liu XH, Ren MJ, Liu XT, Shi XQ, Li ML, Li SA, Yang Y, Wang DD, Wu Y, et al.antibodies (Invitrogen, Carlsbad, California, USA). 4′, 6‐Diamidino‐2‐phenylindole (DAPI) (1:106, CST, #4083) was used to stain the nuclei. 2.7 Image acquisition and analysis The stained specimens were imaged

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