DAPI from Cell Signaling Technology

Product Specs
ItemDAPI
CompanyCell Signaling Technology
Price $74.00 Inquire
Catalog Number4083S
Quantity1 mg
Research AreaRelated Products
ApplicationsIF-IC, FC-FP

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(81) Therapeutic potential of targeting the NEDD4L-eEF1A1 axis in cancer therapyActa Biochimica et Biophysica SinicaJune 25, 2025Yan Qin, Xinyue Wang, Minghui Zhao, Yuehui Liu, Huiyi Hou, Tingting Wang, Yuhe Pei, Jingxin Zhang, Zhou Shen, Feixiang Wu, et al.and mouse IgG were used. The nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI) (4083S; Cell Signaling Technology), and fluorescence images were captured via an Olympus FV3000 confocal microscope

(82) Viscoelastic N‑cadherin-like interactions maintain neural progenitor cell stemness within 3D matricesNat CommunJune 5, 2025Michelle S. Huang, Bauer L. LeSavage, Sadegh Ghorbani, Aidan E. Gilchrist, Julien G. Roth, Carla Huerta-López, Esther A. Mozipo, Renato S. Navarro, Sarah C. HeilshornFluor 647 (1:500, Invitrogen, A-21449). 4′,6-diamidino-2-phenylindole (DAPI; 1 μg/mL, Cell Signaling, 4083s) was included in the secondary antibody solution to stain nuclei. Samples were incubated overnight at

(83) Cleavage of CAD by caspase-3 determines the cancer cell fate during chemotherapyNat CommunMay 30, 2025Jingsong Ma, Jiabao Zhao, Chensong Zhang, Jinshui Tan, Ao Cheng, Zhuo Niu, Zeyang Lin, Guangchao Pan, Chao Chen, Yang Ding, et al.(BSA) in PBS at room temperature for 1 h before incubation with the primary antibodies against DAPI (#4083, CST) and Phospho-Histone H2A.X (Ser139) (#9718, CST) at 4 °C overnight. After dewaxing and receiving

(84) PML Regulated HIF1AN Ubiquitination and Activated PI3K/AKT Pathway to Promote Bone Marrow Mesenchymal Stem Cells Osteogenic DifferentiationInternational Journal of Stem CellsMay 30, 2025Xian-Pei Zhou, Qi-Wei Li, Zi-Zhen Shu, Yang Liuantibody (1：500, ab150077; Abcam) at 37℃ for 30 minutes. Finally, nuclei were counterstained with DAPI (4083; Cell Signaling Technology) for 10 minutes and images were obtained by a confocal microscope (Fluoview

(85) A retinoic acid:YAP1 signaling axis controls atrial lineage commitmentCell reportsMay 27, 2025Abraham E, Kostina A, Volmert B, Roule T, Huang L, Yu J, Williams AE, Megill E, Douglas A, Pericak OM, et al.6434;RRID:AB_11220428 CHCHD2,Abcam,Cat # ab220688;RRID:AB_369572 DAPI,Cell Signaling Technologies,Cat # 4083S;RRID:AB_3695726 GATA3,R & D Systems,Cat # AF2605-SP;RRID:AB_2108571 GAPDH,Protein Tech,Cat # 60004-1-Ig;RRID:AB_2107436 HAND1,Invitrogen,Cat

(86) LDHA-mediated YAP lactylation promotes the tumor progression of hepatocellular carcinoma by inducing YAP dephosphorylation and activationBiol DirectMay 26, 2025Xiaoyong Wei, Long Zou, Yanqing Huang, Chuan Qiu, Guang Cheng, Ye Chen, Jun RaoUSA, 7076 V) were added and incubated for 1 h. The cells were counterstained with DAPI (CST, MA, USA, 4083 S) and observed under a microscope. In vivo tumor xenograft study 24 five-week-old female nude mice

(87) BACE1 Inhibition Protects Against Type 2 Diabetes Mellitus by Restoring Insulin Receptor in MiceInternational Journal of Molecular SciencesMay 26, 2025Tingting Lin, Ting Liang, Yong Shen, Feng GaoAnti-rabbit Alexa Fluor® 555 secondary antibody (A-11004, Invitrogen, Carlsbad, CA, USA) containing DAPI (4083, Cell signaling technology, Danvers, MA, USA) for 1 h at room temperature and washed 5 times in PBS.

(88) Inhibition of xanthine oxidoreductase with febuxostat, but not allopurinol, prevents inflammasome assembly and IL-1β releaseLife Sci AllianceMay 21, 2025Lauar de Brito Monteiro, Anne-Sophie Archambault, Lucas F Starchuk, Armando Alcazar, Ju Hee Oh, Joshua A Dubland, Bojana Rakić, Annette E Patterson, C Bruce Verchere, Ramon I Klein Geltink647 Conjugate, #4414; Cell Signaling). Samples were rinsed with TBS+0.1% Tween, and DAPI (1 μg/ml; #4083; Cell Signaling) was added for 5 min. Samples were rinsed and resuspended with PBS for image acquisition

(89) Antisense oligonucleotides targeting IRF4 alleviate psoriasisActa Pharmaceutica Sinica BMay 14, 2025Yanxia Yu, Yirui Wang, Weiwei Chen, Chang Zhang, Zhuo li, Jing Yu, Minhao Wang, Can Song, Sihao Yan, Jiayi Lu, et al.dilution; BioLegend) at 4 °C away from light. The nuclei were stained with 4′,6-diamidino-2-phenylindole (#4083S; Cell Signaling Technology). The slides were sealed and images were captured at 40 × using a confocal

(90) Endothelial TIE2 Mutation Induced Contraction Deficiency of Vascular Smooth Muscle Cells via Phenotypic Transition Regulation in Venous MalformationsInt J Med SciMay 7, 2025Zhong Du, Fan Yu, Yuan He You, Zhi Yang Zhao, Zhuo Wei Tian, Meng Xiao, Yan An WangAffiniPure Donkey Anti-Mouse, Jackson, USA), overnight at 4 ℃, and nucleus was stained with DAPI (1:200, 4083, CST, USA). Images were finally scanned by an inverted fluorescence microscope (Nikon, Tokyo, Japan,

(91) Identification of potential anti aging drugs and targets in chronic kidney diseaseSci RepMay 3, 2025Qian Zhang, Bing Bai, Lidan Ran, Wei Zhangwere counterstained with 4′,6-diamidino-2-phenylindole (DAPI) (1:1000, Cell Signaling Technology, #4083) for 5 min. Fluorescence images were captured using a confocal laser scanning microscope (Olympus FV3000)

(92) Cardamonin suppresses mTORC1/SREBP1 through reducing Raptor and inhibits de novo lipogenesis in ovarian cancerPLoS OneMay 2, 2025Peiguang Niu, Danyun Li, Huajiao Chen, Yanting Zhu, Jintuo Zhou, Jinhua Zhang, Ying Liu(Alexa Fluor® 488 Conjugate) (#4408, CST) at room temperature for 60 min in dark, followed by DAPI (#4083, CST) staining at room temperature for 5 min. The slides were photographed under a fluorescence microscope. Raptor

(93) Soluble CD27 differentially predicts resistance to anti-PD1 alone but not with anti-CTLA-4 in melanomaEMBO molecular medicineMay 1, 2025Sam I, Benhamouda N, Biard L, Da Meda L, Desseaux K, Baroudjan B, Nakouri I, Renaud M, Sadoux A, Alkatrib M, et al.reagents" CF594,Biotium,92174 CF680R,Biotium,92196 CF430,Biotium,96053 CF555,Biotium,92214 DAPI,Cell Signaling Technology,4083S Software,Software,Software inForm image analysis software,Akoya, HALO,Indica Labs, RStudio,Posit

(94) Human MAPT knockin mouse models of frontotemporal dementia for the neurodegenerative research communityCell Reports MethodsApril 21, 2025Takahiro Morito, Mohan Qi, Naoko Kamano, Hiroki Sasaguri, Sumi Bez, Martha Foiani, Karen Duff, Seico Benner, Toshihiro Endo, Hiroshi Hama, et al.tyramide-rhodamine solution for 10 min. Finally, the sections were stained with DAPI (Cell Signaling Technology #4083S) diluted in TNB buffer before mounting with ProLong Gold Antifade Mountant (Invitrogen, P36934). The

(95) Drosophila SPARC Collagen IV chaperone-like activity essential for development is unique to the fat bodyiScienceApril 18, 2025Samuel Delage, Arya Zadhoosh, William You, Theodore Joseph Brown, Maurice Joseph Ringuette#7074; RRID: AB_2099233 "DAPI (4′,6-diamidino-2-phenylindole)",Cell Signaling Technology,Catalog #4083 Rhodamine Phalloidin,Thermofisher Scientific,Catalog #R415 "Donkey anti-Mouse IgG (H + L) Highly Cross-Adsorbed

(96) NID2 Affects Prognosis of Glioma via Activating the Akt Signaling PathwayInt J Mol SciApril 18, 2025Zhangzhang Lan, Yanlin Xiao, Youyou Liao, Xuan Li, Yi Zhang, Huajie Wang, Wenyong Zhang(Beyotime, Shanghai, China, C0075S). The cell nuclei were stained with DAPI (Cell Signaling Technology, #4083, 1:10,000) for 5 min. The proportion of cells that incorporated EdU was determined through fluorescence microscopy

(97) IL-6 Affects Liver Metabolic Abnormalities Caused by Silicon Exposure by Regulating the PKC/YY1 Signaling PathwayGenes (Basel)April 16, 2025Hui Zhao, Huihui Tao, Jian Gao, Jingjing Wang, Guangliang Hui, Ye Zhu, Jialin Wang, Xuansheng Ding, Yong Daicontinued for 1 h to form the immune complexes. Then, the cell nuclei were stained with DAPI (CST, #4083S) for 5 min. After the samples were mounted with 50% glycerol, images of the samples were observed and

(98) A patient-derived T cell lymphoma biorepository uncovers pathogenetic mechanisms and host-related therapeutic vulnerabilitiesCell Reports MedicineApril 15, 2025Danilo Fiore, Luca Vincenzo Cappelli, Liu Zhaoqi, Nikita Kotlov, Maria Sorokina, Jude Phillip, Paul Zumbo, Liron Yoffe, Paola Ghione, Anqi Wang, et al.AB_2223500 anti-CD20 (clone L26),Leica Biosystems,NCL-L-CD20-L26; RRID:AB_563521 DAPI,Cell Signaling Technology,4083S ,, Biological samples,Biological samples,Biological samples ,, PTCL Patient Samples,"Weill Cornell

(99) Organoid in droplet: Production of uniform pancreatic cancer organoids from single cellsMater Today BioApril 12, 2025Haitao Liu, Tingting Tao, Zhongqiao Gan, Yingying Xie, Yaqing Wang, Yizhao Yang, Xu Zhang, Xianliang Li, Jianhua Qindilution rate of 1:500 for 2 h at room temperature. The cell nuclei were stained with DAPI (1:4000, CST 4083) for 15 min at room temperature. All the images were acquired with the laser scanning confocal microscope. 2.13

(100) The Role of tRNA Fragments on Neurogenesis Alteration by H₂O₂-induced Oxidative StressJ Mol NeurosciApril 11, 2025Bilge Karacicek, Esra Katkat, Leman Binokay, Gunes Ozhan, Gökhan Karakülah, Sermin GencJackson Immunoresearch Laboratories, PA, United States). Nuclear staining was carried out using 4′,6-diamidino-2-phenylindole (DAPI; 4083S, Cell Signaling Technology, MA, United States). Larvae were imaged using a Zeiss LSM880 fluorescence

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DAPI

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