Abcam
Anti-NDUFS3 antibody [17D95]
ab14711
N/A
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Ndufs3 is a core subunit of mitochondrial respiratory complex I involved in the transfer of electrons. I study the effect of different stress conditions and neurodegenerative diseases on mitochondrial respiratory proteins.
Western blot
Human cell lines
1:1000 in 0.5% milk incubated overnight at 4C
5% Milk in Tris-buffered Saline Tween-20
1:2000 HRP-conjugated anti-Mouse IgG, 1 hour at RT
ECL detection developed on X-ray film
The antibody gives a clean single band just below 30 kDa mark for Ndufs3 protein using mitochondria enriched fraction as well as with whole cell lysate .
Do not freeze the antibody. Follow storage instructions carefully.
Good and clean signal. Can be reused 2-3 times.
None.
Recommended antibody.