Anti-NDUFS3 antibodies are used in the immunodetection of the protein NADH:ubiquinone oxidoreductase core subunit S3. In humans, the canonical protein has a reported length of 264 amino acid residues and a mass of 30.2 kDa. Its subcellular localization is in the mitochondria. Alternative splicing is reported to yield 2 different isoforms for this protein. A member of a the Complex I 30 kDa subunit protein family, NDUFS3 is reported to be a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Synonyms for this target antigen include MC1DN8, NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial, CI-30kD, NADH dehydrogenase (ubiquinone) Fe-S protein 3, 30kDa (NADH-coenzyme Q reductase), and CI-30.