Excellent Monoclonal CD3 Marker for Dogs and Cats

November 07, 2026

BlueskyReddit
Afyon Kocatepe University

Overall

Quality of Results

Ease-of-Optimization

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Company:

Agilent Technologies

Product Name:

CD3

Catalog Number:

M7254

Clone Number:

F7.2.38

Info:Supplier Page

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We routinely use CD3 to identify T lymphocytes in canine and feline tissues, particularly in suspected lymphoma cases. Although we have used polyclonal CD3 antibodies for many years, this monoclonal antibody provides cleaner, sharper, and more clearly defined staining.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Cat intestinal lymphoma ( T cell lymphoma)

Primary Incubation

1/25 dilution / 2 hours at RT

Blocking Agent

BSA

Secondary Incubation

Biotinylated Anti-Mouse IgG - 1/200 dilution

Tertiary Incubation

ABC-HRP (1/50 dilution)

Detection

Avidin–Biotin Complex (ABC) Method

Results Summary

This antibody works extremely well in both dog and cat tissues at a 1:25 dilution with antigen retrieval at pH 6.0. Compared with the polyclonal CD3 antibodies we used previously, the staining is sharper and easier to interpret. We are very impressed with its performance in routine diagnostic cases.

Additional Notes

DAB choromagen / Gill's III hematoxylin

Image Gallery

Summary

The Good

Strong and well-defined staining in both species. Better staining clarity than the polyclonal antibodies we used before.

The Bad

nope, maybe low dilution (1/25)

The Bottom Line

An excellent monoclonal CD3 antibody for veterinary pathology. Reliable staining in dogs and cats, even with pH 6.0 antigen retrieval. I highly recommend it.

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