Phospho-AMPKalpha (Thr172) (40H9) Rabbit mAb 2535S from Cell Signaling Technology

Product Specs
ItemPhospho-AMPKalpha (Thr172) (40H9) Rabbit mAb
CompanyCell Signaling Technology
Price $401.00 Inquire
Catalog Number2535S
Quantity100 µl
ApplicationsW, IP, IHC-Bond, IHC-P
ReactivityH, M, R, Hm, Mk, Dm, Sc
ModificationsAMPK AMPK-alpha1 (Thr183) phosphate/AMPK-alpha2 (Thr172) phosphate
HostRabbit
ClonalityMonoclonal
TargetAMPK-alpha1 (Thr183) phosphate;AMPK-alpha2 (Thr172) phosphate
NCBI Full Gene Nameprotein kinase AMP-activated catalytic subunit alpha 2, protein kinase AMP-activated catalytic subunit alpha 1
NCBI Gene AliasesAMPK, AMPK alpha 1, PRKAA, AMPK2, AMPKa1, AMPKa2
Research AreaMetabolism
Clone40H9
Molecular Weight62

Questions and Answers

Can this antibody be used to study AMPK phosphorylation in various cell types?

Yes, the Phospho-AMPKalpha (Thr172) (40H9) Rabbit mAb has been shown to detect endogenous AMPKα only when phosphorylated at threonine 172 across multiple species, including human, mouse, rat, and non-human primates. This specificity allows for its application in various cell types and experimental conditions.

Are there any studies that demonstrate the use of this antibody in cancer research?

Yes, the antibody has been utilized in studies investigating the role of AMPK in cancer. For instance, it was referenced in research examining the regulation of Gasdermin D-mediated pyroptosis in tumor cells, highlighting AMPK's role in modulating cell death pathways ().

How has this antibody been applied in metabolic studies?

The Phospho-AMPKalpha (Thr172) antibody has been cited in studies exploring metabolic regulation, such as the investigation of metformin's effects on nickel-induced autophagy and apoptosis in lung epithelial cells, where AMPK activation was a key focus ().

Is this antibody suitable for use in immunohistochemistry applications?

Yes, the Phospho-AMPKalpha (Thr172) (40H9) Rabbit mAb is suitable for immunohistochemistry applications, as indicated in its product description. It has been effectively used in various studies to visualize AMPK phosphorylation in tissue samples, contributing to our understanding of metabolic signaling in different contexts.

Cell Signaling Technology

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) miR-17-5p inhibits classical swine fever virus replication by targeting the PKD2 -regulated AMPK/mTOR autophagy pathwayVirulenceDecember 31, 2026Xinxian Wang, Shurui Yang, Lichun Xie, Shanshan Qi, Libo Gao, Yongmei Li, Qian Li, Junlong Bi, Jianping Liu, Yongneng Li, et al.PolyAb,proteintech,"10,929–2-AP" Anti pmTOR mouse mAb,proteintech,"67,778–1-lg" Anti pAMPK rabbit mAb,Cell signaling,2535T Anti LC3B rabbit mAb,Nature,A27936 Anti P62rabbit PolyAb,proteintech,"18,420–1-AP" Anti CSFV E2 (IF)mouse

(2) Global and mitochondrial choline dehydrogenase contribute to goose fatty liver formation by differentially regulating energy metabolism, autophagy and oxidative stress related pathwaysPoultry ScienceOctober 1, 2026Xiaoyi Zhou, Mengqing Lv, Ya Xing, Xiangli Zhao, Meiyi Mao, Minmeng Zhao, Daoqing Gong, Chuanju Liu, Tuoyu Geng, Jing Ge(81115-1-RR, Proteintech, China), PAN-AMPKα (2532, Cell Signaling Technology, USA), pAMPKα1/2 (T172) (2535, Cell Signaling Technology, USA), Bax (50599-2-Ig, Proteintech, China), SOD2 (24127-1-AP, Proteintech,

(3) A bitter melon natural compound ameliorates the myotonic dystrophy type 1 skeletal muscle phenotype in a sex-specific mannerNeurotherapeuticsAugust 3, 2026Shatha A. Atieh, Chimène Fahmi, Jia Liu, Chunping Tang, Yang Ye, Aymeric Ravel-Chapuis, Bernard J. JasminTechnology (CST) Cat# 2532, RRID: AB_330331" Phospho-AMPK,WB: 1000,"Cell Signaling Technology Cat# 2535, RRID: AB_331250" CUGBP1-HRP-conjugated (CELF1),WB 1:500,"Santa Cruz Cat# sc-20003, RRID: AB_2292334" MBNL1,WB

(4) Mitochondrial control of amino acid catabolism by a fasting-inducible mitochondrial carrierSci AdvJuly 31, 2026Satoshi Oikawa, Tadashi Yamamuro, Hiroshi Nishida, Daisuke Katoh, Dandan Wang, Masanori Fujimoto, Shingo KajimuraProteintech), ATP5A (ab14748, Abcam), Total OXPHOS (ab110413, Abcam), AMPK alpha (#2532, CST), p-AMPK alpha (#2535, CST) and GAPDH (2118S, CST). Anti-Rabbit IgG, HRP-Linked antibody (ab6721, Abcam) and anti-mouse IgG

(5) Targeting Tex10 Overcomes Oxaliplatin Resistance by Competitively Disrupting the Non-Canonical BAF Complex in Colorectal CancerAdv Sci (Weinh)July 29, 2026Ping Xu, Jie Huang, Xiaojin Gao, Lin Deng, Yanheng Deng, Denglu Wang, Chunlei Yu, Yunxiang Zhang, Jingdong Li, Xiaocong Xiang(Santa Cruz, sc‐398204; 1:250), Anti‐AMPK (HuaAn, ET1608‐40; 1:2000), anti‐phospho‐AMPK (Thr172) (CST, 2535; 1:1000,), Anti‐mTOR (HuaAn, ET1608‐5; 1:1000), anti‐phospho‐mTOR (Ser2448) (HuaAn, HA600094; 1:1000),

(6) Effects of cold water immersion versus active recovery on oxidative stress, redox gene expression and mitochondrial adaptations after strength trainingExp PhysiolJuly 22, 2026Llion A. Roberts, Chantal A. Pileggi, Nina Zeng, Vandre C. Figueiredo, James F. Markworth, Truls Raastad, David Briskey, Katsuhiko Suzuki, Jeff S. Coombes, David Cameron‐Smith, et al.the following proteins: phosphorylated (p) adenosine monophosphate‐activated protein kinase (p‐AMPK; #2535; Cell Signaling, Beverly, MA, USA); total (t)‐AMPK (#2603; Cell Signaling); PGC‐1α (#Ab3242; Millipore,

(7) 2-O-Methylhonokiol Suppresses 3T3-L1 Adipogenesis Through Metabolic Stress Signaling and Early Cell Cycle RestrictionInt J Mol SciJuly 22, 2026Minghao Fu, Manish Kumar Singh, Gyuhui Kim, Kyung-Sik Yoon, Sung Soo Kim, Joohun Ha, Insug Kang, Wonchae Choe(Danvers, MA, USA): PPARγ (2443S), C/EBPα (2295S), XBP1s (83418S), AMPKα (2532S), phospho-AMPKα Thr172 (2535S), mTOR (2983S), phospho-mTOR Ser2448 (5536S), FAS/FASN (3180S), AKT (5298M), phospho-AKT Ser473 (4060S),

(8) Metformin dual-targets metabolism and survival pathways in BPDCNiScienceJuly 17, 2026Zineb Mekkaoui, Ludivine Dal Zuffo, Mathieu Vetter, Maxime Fredon, Margaux Poussard, Sabeha Biichle, Virginie Mougey, Patricia Mercier-Letondal, Gwenaël Rolin, Yann Godet, et al.Dye,Startious,Cat#4632 p-Akt,Invitrogen,Cat#44-621G; RRID: AB_2533699 p-AMPKα,Cell Signaling,Cat#2535 S; RRID: AB_331250 p-mTOR,Invitrogen,Cat#44-1125G; RRID: AB_2533580 p-NF-kB,Invitrogen,Cat#MAS-15160;

(9) SNF1/AMPK controls its own localization by phosphorylating its activating kinase Sak1iScienceJuly 17, 2026Hind Moukham, Marco Caligaris, Pauline Six, Francesco Padovani, Kurt M. Schmoller, Claudio De Virgilio, Raffaele Nicastro, Farida Tripodi, Paola Coccetti3661; RRID: AB_330337 Rabbit polyclonal anti-AMPK-pThr172 (1:1′000),Cell Signaling Technology,Cat# 2535; RRID: AB_331250 Rabbit polyclonal anti-Cdc34 (1:5′000),Coccetti lab,Coccetti et al.58 Goat anti-mouse

(10) Electron transport chain complex I and mitochondrial fusion regulate ROS for differentiation in Drosophila neural stem cellsStem Cell ReportsJuly 14, 2026Rahul Kumar Verma, Atharva Bhingare, Dnyanesh Dubal, Richa RikhyAB_638863 "Rabbit Monoclonal anti-AMPK-alpha, phospho (Thr172) (1:200)",Cell Signaling Technology,Cat# 2535; RRID: AB_331250 Rat monoclonal anti-Miranda (1:400),Abcam,Cat# ab197788; RRID: AB_2936368 Rabbit

(11) A loss of the cytosolic branched-chain aminotransferase, BCATc, enhances Th1 differentiation and skews Tregs to acquire a Th1-like phenotypeImmunometabolism (Cobham)July 13, 2026Tanner J. Wetzel, Christie M. Adam, Tanner J. Kelm, Anna E. Kotula, Madison E. Fagan, Taha Z. Khan, Anthony X. Diehl, Melissa A. Meyer, Jessica P. Sanchez, Brian H. Ladle, et al.(Ser240/244, Cat # 5364), the AMP-activated protein kinase α (AMPKα, Cat # 2603), P-AMPKα (Thr172, Cat # 2535), and β-tubulin (Cat # 2128), were purchased from Cell Signaling Technology (Denver, MA, USA). After

(12) Proteomic Analysis Reveals AMPKα-Mediated Antiandrogen Resistance via Ferroptosis Suppression in Prostate CancerCancer SciJuly 13, 2026Masaki Shiota, Tokiyoshi Tanegashima, Takahiko Hajime, Miho Ushijima, Shigehiro Tsukahara, Shohei Nagakawa, Satoshi Kobayashi, Takashi Matsumoto, Masatoshi EtoAnalysis Antibodies against FTH1 (#4393), GPX4 (#52455), xCT (#15083), Keap1 (#8047), AMPKα (#5832), p‐AMPKα (#2535), LKB1 (#3050), p‐LKB1 (#3482), CaMKK2 (#16810), and p‐CaMKK2 (#35392) were purchased from Cell Signaling Technology

(13) Tirzepatide Attenuates Wire Injury-Induced Arterial Remodeling in Non-Diabetic and Diabetic Mice: Comparison with SemaglutideBiomedicinesJuly 11, 2026Yusaku Mori, Naoya Osaka, Michishige Terasaki, Hironori Yashima, Tomomi Saito, Daiki Tanno, Madoka Ogino, Makoto Ohara, Sho-Ichi Yamagishiphosphorylated AMPKα at Thr 172 (p-AMPK; 1:2500; molecular weight 62; RRID: AB_331250; catalog no. 2535; Cell Signaling Technology Japan, Chiyoda, Tokyo, Japan); total AMPKα (1:5000; molecular weight 62;

(14) AMPK Suppresses Multiple Forms of Cell Death Including Disulfidptosis in Tumor-Associated Macrophages During Tumor ProgressionInt J Mol SciJuly 9, 2026Ruixuan Wang, Huan Wang, Dianyuan Zhao, Wenting Yang, Di Liu, Li TangMA, USA), GPX4 (1:1000, Cat#ab125066, Abcam Technology, MA, USA), Phospho-AMPKα (Thr172) (1:1000, Cat#2535, Cell Signaling Technology, Danvers, MA, USA), AMPKα (1:1000, Cat#2532, Cell Signaling Technology, Danvers,

(15) Molecular mechanisms of autophagy disorder in diabetic neuropathy: Focusing on signaling pathways and regulation of lipid metabolismPLoS OneJuly 9, 2026Li Song, Lin Zhou, Weiwei LiCell Signaling Technology, Cat#5536), total mTOR (1:1000, Cat#2983), phospho-AMPK (Thr172, 1:1000, Cat#2535), total AMPK (1:1000, Cat#5831), phospho-AKT (Ser473, 1:1000, Cat#4060), and total AKT (1:1000, Cat#4691).

(16) A novel small-molecule inhibitor TD6 targets prohibitin 1 (PHB1) and suppresses colorectal cancer pulmonary metastasis by destabilizing mitochondrial complex IRedox BiolJuly 8, 2026Kai Wang, Pengfei Xu, Yu Hu, Dengdeng Ji, Gui Shao, Mengxi Chen, Ziyan Yang, Yujing Yang, Yinshuang Chen, Xinyu Chen, et al.China). For the evaluation of kinase signaling pathways, primary antibodies against p-AMPKα (Thr172) (#2535, Cell signaling technology, USA), AMPKα (#2795, Cell signaling technology, USA), p-ACC (Ser80) (#3661,

(17) Inhalable ROS-Responsive Nanospray Activates PPAR-γ to Restore Macrophage Mitochondrial Homeostasis and Attenuate Radiation-Induced Lung InjuryAdv Sci (Weinh)July 8, 2026Mingquan Gao, Xudong Yu, Ziqian Shang, Mengyao Tan, Xie Huang, Zaizhi Du, Xiaojiao Wang, Xinrui Yang, Ximei Luo, Weidong Wang, et al.(56‐2061‐82) were provided by Proteintech (Rosemont, IL, USA). Antibodies targeting IL‐1β (12242S), p‐AMPK (2535S), mTOR (2972S), and TOMM20 (42406S) were purchased from Cell Signaling Technology (Danvers, MA, USA).

(18) MAM-Localized MANF Counteracts Microinflammatory Stress to Attenuate Mitochondrial Dysfunction and Cataractogenesis in High MyopiaAdv Sci (Weinh)July 8, 2026Xin Liu, Hao Li, Ching Kang, Dongling Guo, Shuyu Liu, Jiaqi Meng, Zhiqian Huang, Yu Du, Xiangjia Zhuanti‐PERK (1:500, Proteintech, 24390‐1‐AP), anti‐phospho‐AMPKα1 (1:1000, Cell Signaling Technology, 2535S), anti‐phospho‐eIF2α (1:1000, Cell Signaling Technology, 3398), anti‐p62/SQSTM1 (1:20 000, Abclonal,

(19) ZRSR1 protects against obesity-associated hepatic lipogenesis independent of minor intron splicingLife MetabJuly 8, 2026Yinkun Fu, Hongyong Song, Yinglin Han, Ding Chen, Wenyan Li, Ying Sun, Ming He, Xin Peng, Xu-Yun Zhao(20543-1-AP), LAMIN A/C(10298-1-AP), and NF-κB p65 (65107451-1-AP) were from Proteintech. Phospho-AMPK (2535S), AMPK (2532), and Phospho-NF-κB p65 (3033) were from Cell Signaling Technology. RNA-seq data analysis RNA-seq

(20) The yeast mitochondrial porin represses Snf1/AMP kinase signaling to attenuate viral replicationGENETICSJuly 6, 2026Sabrina Chau, Serena Marek, Aayushee Khanna, Janhavi Sathe, Sunil Laxman, Marc D MeneghiniAbcam), 1:2,000 anti-L-A Gag (obtained from Reed Wickner), and 1:1,000 anti-Phospho-AMPKα (Thr172) (2535; Cell Signaling Technology). To quantify the relative fold change of protein levels, band intensities

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(1)

12/27/2022p-AMPK Antibody Works Well Mikyoung YouWe measured the p-AMPK level in HFD-fed mouse liver. 20ug of liver lysate was applied and p-AMPK was incubated overnight.
(1)

6/14/2022Great pAMPKa Antibody for Western Blot Daniel HamiltonI seed the cells and I wait 24 hours leaving them at 37 °C, 5% CO2. I stimulated the cells for 24 hours and I harvested with lysis buffer and protease inhibitor cocktail 1:100 (v/v). The antibody was ... Read Review
(1)

10/28/2020P-AMPK for Western Blot Kumar SubramaniWhen heavy bleeding occurs, there’s not enough blood flow to the organs in your body. Blood carries oxygen and other essential substances to your organs and tissues. When heavy bleeding occurs, these ... Read Review
(1)

12/30/2019Extremely Reliable and Consistent Matt SunIf the Thr172 site is where you are interested in looking at for western, I believe this antibody is perfect for you.
(1)

1/15/2016Good Phospho-AMPK Antibody Franz RicklefsI am investigating the role of the AMPK-Pathway after extracellular vesicle treatment. I chose this product since I am also using the total AMPK antibody from Cell Signaling and it is showing good ... Read Review

Phospho-AMPKalpha (Thr172) (40H9) Rabbit mAb

Phospho-AMPKalpha (Thr172) (40H9) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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Phospho-AMPKalpha (Thr172) (40H9) Rabbit mAb from Cell Signaling Technology

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