Biorad
iScript™ cDNA Synthesis Ki
170-8891
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We extract RNA from large numbers of mouse retina samples and need to use reverse-transcription PCR to make cDNA from this RNA. The cDNA is then run on qPCR to detect the expression of our target genes.
Make pure cDNA from RNA samples.
RNA from mouse retina samples
RNA is first treated with DNAse and incubated at 37 degrees celcius for 20 minutes. Then a stopping buffer is added and the samples are spin for 2 minutes. The liquid is removed and added to the master mix made from the kit reagents (kit has recipe). Samples are run on thermo cycler according to the reaction protocol. Samples are then treated to RNAse H (1uL into 20uL of the sample) and incubated at 37 degrees celcius for 30 minutes.
Make all RNA into cDNA instead of splitting the same so there is no variability.
We made clean and stable cDNA from our RNA samples quickly and efficiently. The samples will not contain any RNA or genomic DNA contaminants.
N/A
Quick and easy to use
None
This kit works very well and efficiently to convert RNA into more stable cDNA for later qPCR.
Product Name: iScript cDNA Synthesis KitSupplier Page