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Author Profile
Rebecca G
Lab Technologist
Johns Hopkins University
Articles by Rebecca G
Antibody User Review
Great Mouse Macrophage Antibody
January 17, 2017
Our lab wants to image the macrophages in the mouse retina and we used this primary antibody with different secondary markers to ...
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Assay Kit/Reagent User Review
Great SYBR Green for qPCR
January 10, 2017
We isolate RNA from whole mouse retinas and then make cDNA to examine expression of various genes. We use this SYBR Green ...
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Assay Kit/Reagent User Review
Good Assay Kit To Visualize Phosphatase Tagged Molecules
December 29, 2016
In my studies, I needed to visualize genetically ß-galactosidase tagged molecules in mouse tissue with a fluorescent marker in ...
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Assay Kit/Reagent User Review
Great And Clear X-Gal Staining For ß-Galactosidase
December 28, 2016
Our lab has transgenic mice that express ß-galactosidase on specific cell types. We needed to use this X-gal substrate kit to ...
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Assay Kit/Reagent User Review
Great Taq Kit For Conventional PCR
December 27, 2016
Our lab has many genetically modified mouse lines and we need to use conventional PCR to genotype each liter of these animals. ...
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Antibody User Review
Okay CXCR4 Antibody For Cryosections
December 22, 2016
We are interested in the cells involved in the maintenance of normal vascular structure in the mouse retina, and want to see if ...
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Assay Kit/Reagent User Review
Great Beads For Separating Specific Cells
December 21, 2016
Our lab examines the interactions of various cell types in the mouse retina. We wanted to be able to separate specific cells from...
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Antibody User Review
Good Antibody For Immunofluorescence
December 20, 2016
Our lab uses this antibody to visualize CD45+ cells around the vascular structure in the mouse retina using whole mount staining.
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Antibody User Review
Secondary Antibody for IgG (H+L)
December 19, 2016
Our lab studies various cell types in the mouse retina and this antibody is weak with too much background fluorescent to use for ...
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Assay Kit/Reagent User Review
Good Mounting Medium For Cryosections
December 16, 2016
We take frozen sections of mouse whole eyes to examine the different layers of the eye. This vectashield allow us to protect our ...
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Assay Kit/Reagent User Review
Good And Simple Kit For Making cDNA
December 15, 2016
We extract RNA from large numbers of mouse retina samples and need to use reverse-transcription PCR to make cDNA from this RNA. ...
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Antibody User Review
Great Antibody For Staining Vessels
December 14, 2016
Our lab studied abnormal vessel growth in mouse retinas and this antibody is crucial for staining and imaging these vessels ex ...
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Antibody User Review
Okay Antibody To Use For Immunofluorescent Flat Mount Staining
December 13, 2016
We used various primary antibodies to do whole mount staining of fixed mouse retinas.
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Product Review
Efficient and Cost Friendly SYBR Green Mastermix for qPCR
Parijat Sarkar
I study the transcription of endogenous genes regulated by transcription factors, and it's crucial to quantify their expression levels accurately. To achieve ...
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